LOCUS       CP077252                1551 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1381 plasmid unnamed2, complete
            sequence.
ACCESSION   CP077252
VERSION     CP077252.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357523
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 886.95x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:20:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,078
            CDSs (total)                      :: 4,960
            Genes (coding)                    :: 4,811
            CDSs (with protein)               :: 4,811
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 68 of 149
            Pseudo Genes (incomplete)         :: 88 of 149
            Pseudo Genes (internal stop)      :: 34 of 149
            Pseudo Genes (multiple problems)  :: 38 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1381"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1381"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(1292..1551,1..217)
                     /locus_tag="I6L11_25280"
     CDS             join(1292..1551,1..217)
                     /locus_tag="I6L11_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QXA42710.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(661..882)
                     /locus_tag="I6L11_25285"
     CDS             complement(661..882)
                     /locus_tag="I6L11_25285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA42711.1"
                     /translation="MCKCNTVTRDDIPFAVWSGLRPDTPHQCVRAQCGFRFSSASSPTR
                     SLRSVAGAAASGVTSRMLSVIPLLFFRG"
                     /name="hypothetical protein"
     Typing          822..902
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.122"
                     /coverage="87.0"
                     /evalue="9.59e-28"
     Typing          complement(1105..1213)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(450..708)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="1.09e-111"
ORIGIN
        1 cagaagcaca attattcggt ggctcaatta cttagaatca aaaaatgcat tagttagaat
       61 ccccgttgct ggtaaggttt gtgcgtatgc cctcgatcca catgaagtct ggaagggata
      121 caacactacg aaaaaccatg cagcgtttgt cactaaaaca ctggtcaaca aagacggtga
      181 tattcagcgc cgaatcatgg ccatgttttc aaattgagcc agcggcaggc ggacaatcag
      241 gggctacgtg ttaacgtttt gaccatgatt gtctatcctg cattgctctt ttgccgcctc
      301 aaaatccctt gcttgttttt gctccccgat ccccagaacg accgcgccgc cacggttccg
      361 gcagcgcctt gagcgcaacc ggaaaccgtc tggggagaac cccagacccc catcgctgta
      421 acaccgacgg tctacgacca ccaaccagcc gaatagctgc ctgtgccgtg caaataggct
      481 ttgcaggggg ctgacgcggt tcctagcgac ctgaaagccc ctggttaggg gtatcggcgc
      541 gcctgacctc ctgagagcct ctgtaagcgt ttttcgcgcc ttccatgccc tgacgggcat
      601 ttaaaacccg tttcgtgtgt ctgagagctt ctgagcgcgt ttgaggggct atctggcgac
      661 tcatccccga aagaacaaca acggaatcac cgacaacatg cgagaggtga caccgctcgc
      721 cgcagcccca gcgaccgaac gtagtgagcg agtgggcgag gaagcggaag agaaccggaa
      781 accacattga gcacgtacgc actgatgcgg ggtgtcgggg cgaagccctg accagacggc
      841 aaacggaata tcgtcgcggg tgacggtatt acatttgcac atcctgtccc gatttctgag
      901 gcgttttagc ggtgaacgga cggcaaaacc atgatggagc tgttgagcgt tgagagcgtg
      961 aaatctggca aaaatcacgg ctggcgaggc ttgtagcgga ataagccagt atacactccg
     1021 ctatcgctac gtgactgggt cagggcttcg ccccgacacc ccaaaagggc gttgcgttgc
     1081 gcgcaacacc cttgcctaga atagatcttt tacgccgatt tgtaagttgt tgttttttta
     1141 gtgtgttttt tgtttacctg tcgcatccag ggtgacacaa cgttgtcgca tcctggatgc
     1201 gacaacacgc aacaaggtgt tgcacagatg tgtcatgatg gcttagattt gaaccaacgt
     1261 tagtgcatgg gattttcaga gggaaaaaat catgtttatt gattcagaaa aacgactgaa
     1321 acaactttca gatgaggcaa agaaaaacac cgaggatctc gaagaagcaa agaagaattc
     1381 aaggtttaca caggtatccc caaaaggttg ggaacgcgtt cgagagctgc tgaaggatag
     1441 tcaaggcata tcagcactga agctgtactc atttttagcg gagcatatcg atcctacgtg
     1501 tggcgctgtc gttgcggatc agcaattcct agctgaaaaa cttggagtta g
//
