LOCUS       CP077247                5631 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1376 plasmid unnamed4, complete
            sequence.
ACCESSION   CP077247
VERSION     CP077247.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357518
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 986.60x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:30:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,101
            Genes (coding)                    :: 4,894
            CDSs (with protein)               :: 4,894
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 63 of 207
            Pseudo Genes (incomplete)         :: 137 of 207
            Pseudo Genes (internal stop)      :: 39 of 207
            Pseudo Genes (multiple problems)  :: 26 of 207
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1376"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1376"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(5254..5631,1..489)
                     /locus_tag="I6L06_26100"
     CDS             join(5254..5631,1..489)
                     /locus_tag="I6L06_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="QXA33076.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(1692..1883)
                     /locus_tag="I6L06_26105"
     CDS             complement(1692..1883)
                     /locus_tag="I6L06_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA33077.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1948..2271
                     /locus_tag="I6L06_26110"
     CDS             1948..2271
                     /locus_tag="I6L06_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA33078.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2213..2971
                     /locus_tag="I6L06_26115"
                     /pseudo=""
     CDS             2213..2971
                     /locus_tag="I6L06_26115"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2949..3479
                     /locus_tag="I6L06_26120"
     CDS             2949..3479
                     /locus_tag="I6L06_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXA33073.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            3486..3719
                     /locus_tag="I6L06_26125"
     CDS             3486..3719
                     /locus_tag="I6L06_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA33074.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4200..5252
                     /locus_tag="I6L06_26130"
     CDS             4200..5252
                     /locus_tag="I6L06_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA33075.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     Typing          1517..1598
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          873..1060
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          2262..3823
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.808"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ataggtgcaa ttgttggcag tattttggta gccttgccag acatcagtaa tggatcgaat
       61 aatattgttg gatattgtct tgttgttgcc ggtgtgttat cggcagcatt ttatgttgtt
      121 atatcgtctc gccttattaa atcgtttgag cctatcacat tattgagcgg acagttgaca
      181 ttctccctta tactgtcatc gctctttatt tttatatcta aatcatcaat ttatcttccg
      241 ttggaatccg tgattattgt tttttcatct ggaatattac aatatttttt agctttttgt
      301 ttttacttga actccttgcg ttggctccca gttcatttgg cagggataat gttgtacttt
      361 attccgctag ttgcattatt tttgtcatgg ttgtttttgg atgaacaatt cacattctta
      421 cagggggtag gatttattgt aactgtgcta agtgtatatt ttcttaacag aaaatactca
      481 gaggagtaac attctgttca ggattgcccc ccggcctcca tcgataccgt gccaaatgca
      541 cgtaaaaaac gggagcgttt ctggtgtcgg aatgtaacca agggggtcag aggcgcgcta
      601 aaacgctctc aggggagtca aatagagacg tttgtaacaa aaagatcaaa ggatcctctt
      661 gagatccttt ttttctgcgc gtaatctgct gcctgtaaac gaaaaaaccg ccttgcaggg
      721 cggtttgatc gaaggttaag tcagcttgca gactgcttaa ccaggtaact gtcttatgcg
      781 agacgagtca ccaaaattgt tcttctagtg tagccgcact ccggcagcca cttcaagtac
      841 tctctaaata tctcgcacat gacttttacc aatggctgct gccagtggcg ctttgtggtg
      901 tcttttccgg gttggaccca agagaacagt taccggagaa ggcgcagcag tcgggctgaa
      961 cggggggttc ctgcatacag cccagcccgg agcgaactgc ctaagtcgcc ccgagatacc
     1021 agaagacgtg agctatgaga aagcgccacg tgtcccgtaa gggagaaagg cggaaaaggt
     1081 atccggtaat cgatatggcg ggaacgtggg ggcgcaggag ggagcgattc accggaaacg
     1141 gtgatgatct ttaagccacg tcgggtttcg ccaccgctgg cggatttcat ggttgagccg
     1201 caaggctccc acagatgcac cgaaaaagtg tctgttttgt gaagtccgtc aggggggcgg
     1261 agcctatgga aaaagcgcct ccggcgcggc ctttcccggg gtttcgtttt ttgcgcacat
     1321 gttctttcct ggtttatccc cgggttctgt ggataacagg gttagcgttt ttgagaaagg
     1381 agagtccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
     1441 agagcgcctg aactggcatt ttctccttac gcatctgtgc ggcatttcac accggacatg
     1501 gcgcactttt catacaatcc gcactgatgc cgaatggtta agccagtata cactccgcta
     1561 tcgctacgtg actgggtcag ggcttcgccc cgacacccca aaggtcaacg gcctgacgcg
     1621 ccctggcggg cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg
     1681 agctgcatgt gtcagcggtt ttcaccgtca tccccgaagc gtgcgaggca actgcggtaa
     1741 agctcatcgg cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagt
     1801 tcgttgagct tctccagcag cgttaatgtc tggcctctga tgaagcgggc catgttaagg
     1861 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggggttct gttcatgggg
     1921 gtaatgatac cgatgaaacg cgagaggatg ctcacgatac gggttactga tgatgaacat
     1981 gcccggttac tggaacgctg tgagggtaaa caactggcgg tatggatgcg gcgggtctgc
     2041 ctgggtgagc cggttgcccg ttccgggaag ctgccgacac tggcaccgcc gttactgcgt
     2101 cagctggccg ccatcgggaa taaccttaac cagacagcga ggaaggtgaa cagcgggcag
     2161 tggtcttccg gtgaccgggt tcaggtggtg gccgcactga tggccatcgg ggatgagctg
     2221 cgccggctgc gtctggcggt cagggaacag ggggcgcggg atgatagtta agtttcatgc
     2281 caggggaaaa ggtggtggca gtggtccggt tgattacctg ctgggaaggg agcgtaaccg
     2341 ggaaggggca acggtgcttc aggggaatcc ggaagaggtc cgggaactca tcgatgccac
     2401 gccatttgcg aagaaataca cgtcaggtgt tctgtcgttc gcggagaagg agctgccgcc
     2461 tggagggcgt gaaaaagtga tggcgagctt tgagcgtgta ctgatgcccg gtctcgagaa
     2521 aaatcagtac agcatcctgt gggtggagca ccaggacaag ggacggcttg agctgaattt
     2581 tgtcattccg aacatggagc tacagaccgg aaaacgcctc cagccgtact acgaccgggc
     2641 agacaggccg agaattgatg cctggcagac gctggtgaat caccattacg gtctgcatga
     2701 ccccaacgcc ctggagaacc gccggacgct gacccttcct gataacctgc cggaaacgaa
     2761 acaggcgctt gctgagggcg tcacacgcgg gatagacgcg ctttaccatg ccggagagat
     2821 aaaaggccgt caggatgtga ttcaggcgct cacagaggcg gggcttgaag tggtcagggt
     2881 gacgcgaagc agtatcagca ttgcagaccc gaacggcggg aggaatatca gactgaaagg
     2941 agcgttttat gagcaatctt ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga
     3001 gagccgaatc tacagagaaa atgctgaacg acgagttcag gaagctcgaa gaatctgtaa
     3061 acagggaact gacctcaaac gaatctttaa tcaggaacgc tatcaacgac catacaacag
     3121 ccctgaaaga actgctggag aggtatcaga aaacgaccgt cgatacgatg gacgcgcact
     3181 ggaaaaccgt actgaaaatg agcgtgaaac gctggctgtg gctgattatc gtctcagtac
     3241 tgatgtttgc cacgaccggg agtctgctgt ggtaccaggg catgaagata aacgcgaata
     3301 tgaatattct ccgcgagcag aaagagagtc tggagaagct gaacgcgaag acatggggcg
     3361 tgcgttatca cgaggacagc aacggacgtt ttctggtgtt gccgaagggg atgaaagccg
     3421 aaacgaactg gacgaaggac aacggcaaac tgaacgcagt gagactggtg caggagtgac
     3481 agagcatgac ggagctggaa agacatttgc tgaacgcatt agagcaactg caacaggact
     3541 atatgcagcg gctgaacgaa tgggagaacg ccttcgtgga attgcagaaa atgttttcgc
     3601 ttacgcaacg ggacaacgcg atgctgaacg ggcgagtcat gcagttgagt cagcaggtgc
     3661 agcacttgag cgagcggaca gaacgcttga gccagttata cagcgagaac aggagataag
     3721 ggatgaacgg ctgatgcagg aacgcgaaca tgagttatct ctggagcggg agcgtcagcc
     3781 ggaaatccag gagcggacac tggatggccc ttcgctggga tggtgaaacc atgaaaaatg
     3841 acgtgttcag tggattaagg cgggctaatg tggcctgtag catctggttg cataggtatt
     3901 catgcggtta aaatttatca ggtgcgatcg cggcagtttt tcgggtggtt tgttgccatt
     3961 tttacctgtc tgctgccgtg atcgcaccga acgcgttttg gcggtgcgta caatgcgacg
     4021 ttatggtaaa tggagcggat tttcaggaag gagtgatact aacaactcga acactgtgca
     4081 aataatgatt tttttaaaat atcggtaacg ataataaatt atttcgtttt acgccggtaa
     4141 taaatataat gtagtttcag tatataaaaa aatatatgag gtgtcttgtg ggagctttca
     4201 tgaatcttca acaacgtata aataaactac cacaattatc atccagtttt tcatttggaa
     4261 aagatattga taatattcat tcatttatct ttaacgaaac ttctaaagat aaaattgaag
     4321 atttactaag aaaatgggtg tcaggcaatc agccctgtgt atttggtaaa ttggcaagca
     4381 agaaaattaa agggcttgat ttccatttat ctattgtaaa tagcccccaa ctatataatg
     4441 atgatggtca tttgtttgat tttttgcgta atgaaagagt tcgttttaaa gaacgcgcaa
     4501 gaagaggaga ggtcagtgcg catctcattt attttattca tccacaactt gcctttgctc
     4561 gtccatcaga agagttagtg gacatacaaa aatatatttg ttcgctgcat atgccagagt
     4621 gctatccaat aaaagaggat gttatttata ctgaaagcgt accatttcag gataaagatg
     4681 ggctaaaaat atataaggct ggtgttaatg ttttttactc ttctgctcat agaacaagaa
     4741 atcatgatag aagaatacca gggggaatac ttatttctgt aaatgccccg gggcatttta
     4801 tgaggctggc gatagaaaaa ggtttctata aagatcaaga acaagcgtta gcggatattc
     4861 gtaacatgac tattcagtct gttggtaagg gtgggtattc acatccggaa gggatttcta
     4921 caacatggca ttcagaatct aatttggatc gttttggatg cccagtgcac accggtaata
     4981 gttcttatta cagcggtttt tatcatactg atgtattaat tccaggagaa ttaactaagg
     5041 atgaaagatt gctccatgaa atagaaaata gtgatccaat gatcttcaac tggaatgtat
     5101 tgttttatgt ttcattagaa gagtttccta tagatgatcc ttattatgga gagtttattg
     5161 gtattcctgt agatgatgca tctatgtttt ttaactcctt tcaaccaagg aaatttgaaa
     5221 acaaacccct ttatgagaaa gaggatgatt aatatgatta aaggaatgct ttttgctttt
     5281 ttagcagcgt tctcatgggg ggcagctatc gtaatgagca aaaaaggagt tgaagaacta
     5341 gatgccggag gggtgttttt ctggcagatt tgtagtgcag taatactatc ttggatagtt
     5401 ctttttataa aaagaaaaaa actcccatta aactcaaaag cgatacttgc ttatgcgaca
     5461 ggcgtttttg aaccattctt agcgtatact tttacactct acggacttta ttttatttca
     5521 gcaggaagcg catcaattat tttttcactt gaatcagtgt ttatattggt gttgtctgtt
     5581 cttatttgct cagttaaaat taattccccg caatatttta tattgttcct t
//
