LOCUS       CP077231                6640 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1384 plasmid unnamed6.
ACCESSION   CP077231
VERSION     CP077231.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357526
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 657.97x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:56:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,845
            CDSs (with protein)               :: 4,845
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 142 of 230
            Pseudo Genes (internal stop)      :: 28 of 230
            Pseudo Genes (multiple problems)  :: 28 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1384"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1384"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(351..506)
                     /gene="csl"
                     /locus_tag="I6L14_25970"
     CDS             complement(351..506)
                     /gene="csl"
                     /locus_tag="I6L14_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071703583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 system lysis protein"
                     /protein_id="QXA06201.1"
                     /translation="MKKIIICAISLGIMLLAACQVNNVRDTGGGAVSPSSTVTGVSMGS
                     GGSGSL"
                     /name="csl"
     gene            536..1075
                     /gene="csi"
                     /locus_tag="I6L14_25975"
     CDS             536..1075
                     /gene="csi"
                     /locus_tag="I6L14_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin S4 immunity protein"
                     /protein_id="QXA06202.1"
                     /translation="MMIDEHSIDIDNRKANNLLYLFMAIGVIPLLCILAVYYTNPDNLF
                     LHTIATSTENIPSITSAYNPLMTKVMDIYCKTAPSLALILFILTFKTRKLIKKTNRNAV
                     LRSCLLSPFVCAVFLYLLCFRNLELTTAGRPARLMTNNDATLLLFYIGLYLIIFFISYF
                     TLFTPVTTFKLLKERQ"
                     /name="csi"
     gene            complement(1103..2602)
                     /gene="csa"
                     /locus_tag="I6L14_25980"
     CDS             complement(1103..2602)
                     /gene="csa"
                     /locus_tag="I6L14_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin S4"
                     /protein_id="QXA06203.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGSG
                     NGGREHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGLVKTPVYGVPGAGGNSDVQG
                     GYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLNDNGRAIGILRTGVRPYVGSEK
                     AKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQAEDAWDRLPPNVRKFDVDVEQF
                     HYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKVDHKTPEEIYEALGFNNEEPQRQ
                     DQAKKAAYDVFYSFSMNRDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIADDV
                     KNFQGKTIRSYDDAMASLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLGNISKA
                     FKFADVVMKVEKVREKSIEGYETGNWGPLMLEVESWVLSGIASAVALGVFSATLGAYAL
                     SLGAPAIAVGIVGILLAAVVGALIDDKFADALNKEIIKPAH"
                     /name="csa"
     gene            2841..3131
                     /locus_tag="I6L14_25985"
     CDS             2841..3131
                     /locus_tag="I6L14_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA06204.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPST"
                     /name="hypothetical protein"
     gene            complement(3123..3401)
                     /locus_tag="I6L14_25990"
     CDS             complement(3123..3401)
                     /locus_tag="I6L14_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA06205.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            3523..3945
                     /locus_tag="I6L14_25995"
     CDS             3523..3945
                     /locus_tag="I6L14_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QXA06206.1"
                     /translation="MRSAATRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRAKEFHSEKFFHSLQSTTT
                     FAGRKYTNSDMPSLKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4429..4662)
                     /locus_tag="I6L14_26000"
     CDS             complement(4429..4662)
                     /locus_tag="I6L14_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA06207.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4669..5229)
                     /locus_tag="I6L14_26005"
     CDS             complement(4669..5229)
                     /locus_tag="I6L14_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXA06208.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLIKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(5201..5944)
                     /locus_tag="I6L14_26010"
                     /pseudo=""
     CDS             complement(5201..5944)
                     /locus_tag="I6L14_26010"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5907..6230)
                     /locus_tag="I6L14_26015"
     CDS             complement(5907..6230)
                     /locus_tag="I6L14_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA06209.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6298..6489
                     /locus_tag="I6L14_26020"
     CDS             6298..6489
                     /locus_tag="I6L14_26020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA06210.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDVDDQADICEALHDHA
                     DELYRSCLAHFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2..91)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.802"
                     /coverage="83.0"
                     /evalue="1.52e-36"
     Typing          complement(4323..5917)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.248"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6290..6492)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.765"
                     /coverage="100.0"
                     /evalue="1.38e-10"
ORIGIN
        1 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
       61 tccggtaact atcgtctcga gtccaccccg gtaagacacg acaaagcgcc actggcagca
      121 gccattggta actgaaaagt ggatttagat acgcagaact cttgaagttg aagccttata
      181 gcggctacac tgaaaggaca gcatttggta tctgtgctcc acttaagcca gctacccggt
      241 taagcagtcc ccaactgact taaccttcga ctaaaccgcc tccccaggcg gttttttcgt
      301 ttacagggca agagactacg acgatctgta aaggatctca agaagatcct ttacaggcta
      361 ccactaccac cacttcccat actcactccg gtaacagtgg aagatggtga aacagcccca
      421 cctccagtat ctctgacatt gttgacttgg catgctgcaa gcagcattat tcctagtgaa
      481 atggcacaaa taataatttt tttcatatat acctttatta taatcctata ggtcaatgat
      541 gattgatgaa cattcaatag atatagacaa tagaaaagcc aataacttat tgtatttatt
      601 tatggcaatc ggagttatac ctttactctg tatactagcc gtttactata caaatccaga
      661 caatctgttc ttgcacacaa tagctaccag tactgaaaac atcccatcaa taacctccgc
      721 ctacaaccca ttaatgacaa aggttatgga tatttattgt aaaacagcgc cttcccttgc
      781 tttgatatta tttatcctga catttaaaac gagaaagcta ataaaaaaaa caaaccgaaa
      841 tgctgtactt agaagttgct tattaagtcc atttgtctgt gctgtttttt tgtatttact
      901 ctgtttccga aaccttgagc taacgacagc aggaaggcca gccaggctaa tgaccaacaa
      961 tgatgcaaca ctattgctat tttatattgg cctgtactta atcatttttt tcataagcta
     1021 tttcacgcta ttcacaccag ttaccacgtt taaattatta aaagaaaggc agtaaccctg
     1081 ccttcctttt acccggtatt tattaatgag cgggtttaat gatttcttta tttagtgcat
     1141 ctgcaaactt gtcatcaatt aatgcaccta caactgctgc gagtagaata cctacgatac
     1201 cgacagcaat agcaggagcc ccaagagata aggcatatgc ccctaatgtg gctgaaaaaa
     1261 cacccagggc cacagcagaa gcgatgccac tgagcaccca ggactccacc tccagcatca
     1321 gaggtcccca gttcccggtt tcatagccct cgatgctttt ctcccggacc ttctcaacct
     1381 tcatcaccac atcggcaaat ttgaaagcct tgctgatatt acccagttta tttgccatat
     1441 cctgagcatc aatacttcgc cagacgttcg ccagtgcatc actgtcggcc ctgttaaatt
     1501 taaaccccgg attagacaaa actttattta atgacgccat tgcatcatcg tagctacgga
     1561 tagttttccc ctggaaattt ttgacatcat cagcaatttc tcttgctaac gacttataag
     1621 catctccaag ataatcaccg accttattac ctatatcaga gataacttca gcggctttat
     1681 taagtacatc actctgaatc cggtcacggt tcatactgaa agaataaaac acatcataag
     1741 cagctttttt agcttgatcc tgtcgttgag gctcttcatt attaaatcct aatgcctcat
     1801 atatctcttc cggggtctta tggtcgactt tatccataat accagcctta gctttttcac
     1861 tccctacata aggacgaaca gctgttctag ttacagataa aacattacca taatcatcaa
     1921 gcaccactaa atagtggaac tgttcaacat caacatcaaa ttttctcaca ttaggaggca
     1981 aacgatccca cgcatcttca gcctgttgtt ttgctttttc ctgccgctga gattcatctt
     2041 tattaaaacc taacgcctca tatatctcct caggagtttt atgattgatt ttttccataa
     2101 tacccgcctt agctttttca ctccctacgt aaggacggac acctgttcga agaataccga
     2161 tagctctacc attatcatta agcgttacac gatatttaaa accatcgata ctcacatcaa
     2221 tctctcgcgg taagttgttt ttatcccact tacgagcaac ttcatcatta ggattttcag
     2281 ggatatagcc cccctgaaca tcactatttc cgccagcccc aggaactcca tatacaggcg
     2341 tttttaccag accatttgtg atggtaatgc gcacctgccc attatcatca acataaggcg
     2401 gcacatccgg attattggtt ttactccatc cccaccccgt ctgacttcca tgttcacgac
     2461 ctccattacc agagcctgaa ccaccacccc agtgaacccc actattacta ttattatttc
     2521 caccttgctg attcagattc gcccctgttc cccccataga ctcaccagca gttgggccat
     2581 atccacttag ttctttagcc ataaattcct ctttgataat taaaacaata aattaaaaac
     2641 agaacactgt acatataacc actggtttta tgtacagcaa aacctactac tcactattgt
     2701 ccatgtcaag agcatggatt ttcatttttg ctataaggat cacgctatgg ggaggcaggc
     2761 attgagaacg tcgaaacaga acaccggagc aaatcaggat gagatataaa actgttggat
     2821 catgaaaaac ggagaacgat gtgagcaaat caccccgcca taaactgaac aaaacagaca
     2881 aacgacttct cgacaccctt gttgctgccg gatatgagca tgacaaagcc cgtgacctca
     2941 tccagaaaca ggtttacacg ctgacactgg ctgatcagcg tcatgtggtc agtgaaatca
     3001 gtaatggtgt gaatcccacc caggcttact cggcggtata ccaggcaaga cgcattcgcc
     3061 tcgcccgtaa atatctgaac ggtaaaaagg ttatggaaga aaccggggaa aatacgcccc
     3121 catcaacgta aggatttctt ttgccgctcc agagactcca gttttttacg caaatcctct
     3181 cttttttggg catctctggt gccagccagc tctgctctca actcatcgat ctgaagttgt
     3241 atcttcagtc tgttactgaa cattttctgt ctggcattaa catccgcaac aatgccgttt
     3301 tttgtcttct cggccttttg ttgaaaaaca ttgctgtccg catgactggc aaccgaagca
     3361 gaaagaacac taaaaagcag gactggcaca cattttttca cgggattatt cctgactcat
     3421 tgaccatcaa atcacattgg gagtaaaccg acgtatgata agagatactc ttcggagata
     3481 taactccctg agtatcaaga ttaaaaacgc aaggagatgt ttatgagatc tgccgctacc
     3541 aggctgcttc tgatacctct gataacagca acaatagctc ttacaggatg caccccaaag
     3601 accagcctgg aacgacatac ccgacattat gtttatgctt cagatgatgg atttgatcct
     3661 aacttctata cccagaaagc agacaccata cgtatgatgc tcccgttctt tcagcagttc
     3721 cgggatatgg ggatgaaaga caaagcagcc ggagtatcag cagaaacggc acagcaacgt
     3781 gcaaaagaat tccactcaga aaaatttttt cactcactcc agagcacaac aacctttgct
     3841 ggcagaaaat acacaaacag cgatatgcct tcgctgaaaa aaatgaaact aatggcagac
     3901 accatttctg cggtttatct cgatggatac gagggcagac agtaagggat ttaccataat
     3961 cccttaattg tacgcaccgc tgaaatgcgt tcagcgcgat cacggctgct gacaggtaaa
     4021 aatggcaaca aaccacccga aaagctgccg cgatcgcacc tgataaattt taaccgtatg
     4081 catagctatt cagccatgtg aataacgctg gttttgcctg cgtaaacctc atgacactgt
     4141 tttttttcca tcttttcagt tgatgacata cgcagacatc gcgggatgag gctgaggaat
     4201 gagcgcgatc tggcaaagag gcaaaacaca gcaacaaaaa cgacacgcca gaatcgcgcc
     4261 cggatgcgtt tttaacgcgt tccggtacca tctggcaacc tcccggaaca actcaccgtc
     4321 acatccccat tgacgggcca cgccatacct gtgcttcccg ttcctgctct tcatgccagg
     4381 accgcgcacg ctcccgttcc aggcgtgcct gcctttcctg ttcatccctt atctgctgtt
     4441 cgtgataaat aaccgactca agcggtccac ctgcccggct aatctctgca cctgctgact
     4501 caagtcgtcg cactgttccc tcagttgccc gttctcctgt cgtgtcagct cgaacatatg
     4561 ctgcaaatcc gtgaaggcgc tctcccagtc tttcagccgc tgcgtatagt cctgctgcaa
     4621 ttgctctaag gcgttcagta agtgcatttc cagctctgtc atactcactt actccctgac
     4681 cagtcttact gcgttcttct tctccaccgt ccagttgttt tcccccttca ccccggacgg
     4741 cagcaccaga aatttcccgt tcctgccctc gtgatacgtc acaccccatg ttttttcccg
     4801 gagtttcgcc agcgtctctt cctggtccct gatagccagg atgttcgccg caatccggct
     4861 ttcctgccac tgaatcagcc cccatgacgc cagaaaaatc ccgcccgtga cgcaaagcgc
     4921 cgtcagcgac gggtacagta tccgcccttt gatcagcttc cagagcagct cttcctgccg
     4981 ccgggccagt tcgttctctg tggcgctgaa ctgcgcgttc acggcactgt tcagcgtctc
     5041 cagttgttct ttcaccgctg ctgtgtgtgc gctgatagcg tctctgattt tctgcccgtt
     5101 taagttcagt tccctgtcta cagacgcttc gagcttcctg aactcgctgt tcagcatgtt
     5161 ctctgtagag acggcacgct ctttcagttt cttctcgaag tctgtcccca tttgtaaaag
     5221 attgctcata cagcgcccct ttcagcctga gattacgccc gccctccggg tcggcgatac
     5281 tgatactgct ccgggttgtc ctcacaacct caaaacctgc cgctgtaagc gcctcagtga
     5341 catcctgacg cgtttttagc gctccggcat ggtaaagagc ctccagtccc ctcgtaatcg
     5401 cttctgcggc ctcctgtttc gctttcggca gattattcgg ggtgacaagt gtccgcctgt
     5461 tctccggtgc gttcgggtcg tgcagcccgt aatggtgatt caccagtgtc tgccaggcat
     5521 tgattcgcgg acggtccgcc cggtcgtaat agggctggag ccgttttccg ctcgccagct
     5581 ccatattcgg gatgacaaaa ttcagctcaa gacgcccctt gtcctggtgc tccacccaca
     5641 ggatgctgta ctgatttttt tcaagaccgg gcatcagtac ccgctcaaag ctctccatca
     5701 ccctttcacg ctctcccggt ggcagggtct gctctgcaaa agacagaacc cccgaggtgt
     5761 attttttcgc aaacggcgtg gcatcgatga gttcccgtac ctcttcggga gcaccccgca
     5821 gaactctcgc cccttcccgg ttacgctccc ggcccagcag gtaatcaacc ggaccactgc
     5881 caccgccttt tcccctggca tgaaacttaa ctatcatccc gttctccctg tttacggacc
     5941 tcatccctaa gctcactcag ttcacgtccg atggccatca gtgcagccac cacatgaacc
     6001 cggtcatgcc ccgaccactg tccgctgttt atcttcctgg ctatctgatt caggttattg
     6061 ccgaccgaag cgaactggcg caacagcggc ggtgccagtg tcggaagacc tgacgttttc
     6121 gatggcggtg cccccaggca gaccttacgc atccatgacg caagttgttt tccctcacaa
     6181 cgtgccagca gccgcgcatg ttcctcatcc gtgacccgta tcgtgagcat cctttcgcgt
     6241 ttcaccggta tcattaaaaa cctccgacag actccccaca catggagaaa cagaactgtg
     6301 actaaacagg aaaaaaccgc ccttaacatg gcccgcttca tcagaagcca gacgctgacc
     6361 ctgctggaaa aactgaatga actggacgtc gacgaccagg ctgacatctg cgaagcgctt
     6421 cacgaccacg ctgacgagct ttaccgcagc tgcctcgcac acttcgggga tgacggtgaa
     6481 aacctctgac acatgcagct cccggagacg gtcacagctg gcctgtgagc ggatgccggg
     6541 agcagacaag cccgtcaggg cgcgtcagcg ggttttagcg ggtgtcgggg cgcagccatg
     6601 acccagtcac gtagcgatag cggagtgtat actggcttaa
//
