LOCUS       CP077230                2744 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1384 plasmid unnamed5, complete
            sequence.
ACCESSION   CP077230
VERSION     CP077230.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357526
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 657.97x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:56:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,845
            CDSs (with protein)               :: 4,845
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 142 of 230
            Pseudo Genes (internal stop)      :: 28 of 230
            Pseudo Genes (multiple problems)  :: 28 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1384"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1384"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(2199..2744,1..453)
                     /locus_tag="I6L14_25965"
     CDS             join(2199..2744,1..453)
                     /locus_tag="I6L14_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QXA06200.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGER
                     VGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPKIV
                     TEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFKRLKERKEFRHVLGWVRTTEVTRGK
                     DGTAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRKPKDD
                     ESLPCVVAEQLRGAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQ
                     LERETNEDLIMADDMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
                     /name="protein rep"
     Typing          1085..1160
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.593"
                     /coverage="88.0"
                     /evalue="2.64e-23"
     Typing          complement(171..362)
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.482"
                     /coverage="100.0"
                     /evalue="1.32e-94"
ORIGIN
        1 tccggccgtg agtatgtgaa acatgcccgt tgggtggaac tgtggcgtga ttgtctgcgg
       61 gtggcgtatg acccgaatgt ggatatcaga gtggtgaaat cacggaaacc aaaagacgat
      121 gaatctttac cttgtgttgt tgcggaacag ctgcgcggcg cggtggttga aactctgaaa
      181 tattccacaa agcctgctga tatgctggct gatcctgaat ggttcctcga attaacacgg
      241 caggtacaca agcgtcgttt tgttgctacg gggggagcgc ttaagaacgt tctgcaactg
      301 gagcgggaaa cgaacgagga tttaatcatg gcggatgaca tggcagaggg tacagatgac
      361 ggttcccgtc tggcgttttc gtgggtgccg gttgtcaaaa aataccagcg cgatcgggcg
      421 aaagataaac gtcagctaag tggcgaggat tgagcgcgtt tcaggcgcga actattcatg
      481 cactgggaga atattcagaa acgcgctcag aagcgttcta acgtgatttt cagaaacgag
      541 caaggattta ctcgtttttt gttttgacgc actcagaagc gttttaagcg cgtttccggg
      601 tctctgctac ggcagtccac ggctgacagc ttttcgctca ccagaaacac gcatgtcatg
      661 tttagcgcgg tgtgattctg ctgtccagta gtcggagggg gggggcgtgt ttctacaggt
      721 cttcgtgggt tgatgatagt ggaaatgcga aagaaacacg ctcatccgtt gcttaacaca
      781 aaagatacaa agcaacaaca tttccgcaca tggacaggaa ggggaagtga gcgcatttga
      841 gggcggggtg taacaatccc catttgacgc acagtgaatt ttaacccctc agacgcgctc
      901 agacgcgttc taacgtgatt tttgcgggca ggcaaggttt tgttcgtttt ttcgcgtttg
      961 atgcgttcag gggcgtttta agcgcgtttc tgtcgcatcc cggcgctaat accatcccca
     1021 tggttaatgg gagcttcaga aacgcgcaga tgtcgtttta acgtgtcccg ggcaggttgt
     1081 cgagggtgtc ggggcgtagc cctgaccagg tggtaatcgt atcggcgtgc gcggttatac
     1141 gattacacat cctgtcccgt catcggagtt gtttttgcgg tgttcctgcg gcaaattgag
     1201 cgggcatagc aacacctcaa aacaactatc atcaggaaat tgactttgac ggccccctga
     1261 cacaggggga ttttttttgc ctgttatctg cggttttcac cgcgtcgcag accgcacaca
     1321 atcgaaacga agttgagatt gtataagtgc gcacttcgtg cacttcgtga gattcccccc
     1381 gactgccagt ttgtcgatcg cgcggaacgg ctgcggcacg aaaacggcaa aatgatttgc
     1441 cgtccggttg ccagatctgc gcgcttccgt ggagggcgac acggcgcaaa aactgccgat
     1501 attgcgccag cgttccggta acacaaactc gcagaaatcg ggacaggatg tgggaatgtc
     1561 ggaccgcgca cgcgcactca gacattcccc acctggtcag ggctacgccc cgacaccctg
     1621 ccaacgcttc gcggcacgtg aaaaaattcc gcgtactgct caccgttccc gaattcgtca
     1681 aacagaaaat cgtggggtcc cgcgcctccg gcgtgggttc cgattttgtg attgacgatg
     1741 gcacggaaaa aacactggag gaggtggttg gtgtcacgga gtgatgccaa ccacctgcaa
     1801 gacggttttt gttgttcaca aaattggtgg aaaagcctgt gggtgttttg tcgggaaaac
     1861 gctttcgtgg ttgttgtttt tgccctcaaa aaatcgaaca aaaaacaggc gattttgctg
     1921 ctgctttcgt gcgcgttcct tgtatttata cttaagggat aaatgcgatt ttttgaatca
     1981 gctctatccc ttgccatatc tggcctgagc gcggatttgc gtgtaaatag gtttgagagt
     2041 attttgtcgg gagtggttat aaattagccc ctgcgagtct tccaccactc caggggcgcg
     2101 accacctaga acatgaactg atttgacgac aattggtaca ggtgatacag gaatgataac
     2161 acgcaatttc gtctgtcgct cgcaagggga cggcagacat gaaaaatcct gaacttaaga
     2221 atctgacgga ttactctcca gcagataaac cgtgggatga acaccgaagc ttttccgacg
     2281 atgtgggcgg tatttatctg cgtgctgccg gatttgagca ctacggcgaa cgtgtcgggg
     2341 cttgtagtgg tattttgcgg tttgggtggt caacaaacac agagaccgga gaaacgcgcc
     2401 tgcggttgcg tgatgcacgg ttctgtcgcg tccggcactg tccgatctgt cagtggaggc
     2461 gttccatgat gtggcaagcc cgtttctatc agtccctgcc aaagattgtg acggaacatc
     2521 ccggcgcgcg ctggttgttt ctcacactga cagtgcgcaa ctgttccatt gacgcgcttg
     2581 gtgagacgct gaccgcgatg aatgcggggt ttaagcggtt gaaagagcgt aaggaatttc
     2641 gccatgtgtt gggttgggtt cgtaccacgg aagtaacgcg cggcaaggat ggtactgcac
     2701 atccccattt tcacactctg ctgatggttc ccccttcgat gttg
//
