LOCUS       CP077229                5685 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1384 plasmid unnamed4, complete
            sequence.
ACCESSION   CP077229
VERSION     CP077229.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357526
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 657.97x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:56:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,845
            CDSs (with protein)               :: 4,845
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 142 of 230
            Pseudo Genes (internal stop)      :: 28 of 230
            Pseudo Genes (multiple problems)  :: 28 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1384"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1384"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            join(4600..5685,1..45)
                     /locus_tag="I6L14_25930"
     CDS             join(4600..5685,1..45)
                     /locus_tag="I6L14_25930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="QXA06195.1"
                     /translation="MTVKNNYLNRGYLVTSTTQAKKIAKLHLEKLDLADKFSFGLPEVD
                     DRYHVWRVPLVATGHRIGEIVINSKTSTIDYKKSSKSPFLIKKAMEVVHTPKKVKKEKK
                     IISKSPLNNMLLQGNCAETLKKLPDESVNLVFTSPPYYNAKPEYSEYHTYDEYLSLLRS
                     VIKECHRVLSEGRFFVINVSPVLIRRASRNEASKRIAVPFDLHRLFIEEGYEFIDDIHW
                     VKPEGAGWALGRGRRFAADRNPLQYKPVPVTEYILVYRKKTDKLIDWNIRNHHSKEDVF
                     DSKIGDDYEKTNLWKINPSRNRKHPATFPYGLAERVIKYYSFKNDVILDPFAGSGTTAK
                     AAIDLGRRFVMCEISKQYIDLIIEGTMLGGDLKIIN"
                     /name="site-specific DNA-methyltransferase"
     gene            complement(429..662)
                     /locus_tag="I6L14_25935"
     CDS             complement(429..662)
                     /locus_tag="I6L14_25935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA06196.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(669..1187)
                     /locus_tag="I6L14_25940"
     CDS             complement(669..1187)
                     /locus_tag="I6L14_25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXA06197.1"
                     /translation="MSNLLQTRAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(1168..1923)
                     /locus_tag="I6L14_25945"
                     /pseudo=""
     CDS             complement(1168..1923)
                     /locus_tag="I6L14_25945"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1865..2188)
                     /locus_tag="I6L14_25950"
     CDS             complement(1865..2188)
                     /locus_tag="I6L14_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA06199.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2253..2444
                     /locus_tag="I6L14_25955"
     CDS             2253..2444
                     /locus_tag="I6L14_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA06198.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3456..4523)
                     /locus_tag="I6L14_25960"
     CDS             complement(3456..4523)
                     /locus_tag="I6L14_25960"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfrBI family restriction endonuclease"
                     /protein_id="QXA06194.1"
                     /translation="MNFKDKNCFPNELIALAKISKNDVLDKFGTDVFKKVVYDVLTGKN
                     VREFTEILTRTRLLESNLSFFDFFVDKMKEGITPKQLYLYAKNALSNKSYVKSNQPVLE
                     WMVMMTNKQTQNVLRDEHGDGFDRLALRTQEEILKIKNGYEDKIGEISIGGQKVSLEDF
                     CYIILSLGSQTLTIRGSEKSLHGKYFEKLILGSLFTIMGFEYKEKIEEGLNAKCFTLST
                     RADDRESDATLVFNGKAIRVDIGFIGRGNTEISLDKVSRFRRMDDIGGVMHNISTMVIV
                     DVIGDRSRIVNMAEEIDGKVVAMSDPYWVAKVSSYISSKLNVDDLLEDKPQLKDIQSFI
                     SDALENVDLEKYIKL"
                     /name="CfrBI family restriction endonuclease"
     Typing          complement(2530..2617)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.506"
                     /coverage="100.0"
                     /evalue="2.4800000000000002e-33"
     Typing          2641..3182
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(326..1875)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.183"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atagaaggaa ccatgctagg tggtgattta aaaatcataa attaatcaaa ctttactgtg
       61 gtaaaagccg ccttaaggcg gcttatttac gcgtcaacat cagccacatt gtcgaattta
      121 ccataatccc ttaattgtac gcaccgccaa aacgcgttca gcgcgatcac agcagcagac
      181 aggtaaaaat ggcaacaaac cacccgaaaa actgccgcaa tcgcgcctga taaattttaa
      241 ccgcatgaat acctatgcaa ccagatggta cagaccacat tagcctgtct taatccactt
      301 aacctgccat ttttcatggt ttcaccatcc cagcgaaggg ccatccagcg tgcgttcctg
      361 tatttccggc tgacgctccc gttccaggga taacacatgt tcgcgctcct gtatcagccg
      421 ttcctctctt atctccagtt ctcgctgtat aactggctca agcgttctgt ctgctcgctc
      481 aagtgctgca cctgctgact caactgcatg acccgctcgt tcagcatcgc gttgtcccgt
      541 tgcgtaagcg aaaacatctt ctgcaattcc acgaaggcgc tctcccattc gctcagccgc
      601 tgcatatagt cctgttgcag ctgctctaag gcgttcagca aatgtgtttc cagctctgtc
      661 actctgtgtc actccttcag atgtacccac tctttcccct gaaagggaat cacctccgct
      721 gatttcccgt acggaaggac aaggaatttc ctgttcccgt cctgcacaaa ctccacgccc
      781 catgtcttcg cgttcagttt ctgcaatgtc tcttcctgct tcctgatttc ttccaggttc
      841 gcctgtatcc tccctccaag ataccagagc gtcccgccac tcgctgtaaa caggagaaag
      901 actatcccca gtaacatcat gcccgtattc cctgccagct ttaacacgtc cttcctgtgc
      961 tgcatcatcg cctctttcac cccttcccgg tgtttttcca gcgatttctc tgtcgaggct
     1021 gtgaacaggg ctatagcgtc tctgattttc gtctcgtttg aggtcacagc ctcgctgaca
     1081 gactcgccga gcctcctgaa ctcgttgttc agcattttct ctgtagattc ggctctctct
     1141 ttcagctttt tctcgaactc cgcgcgcgtc tgtaaaagat tgctcataaa atgctccttt
     1201 cagcctgata ttcctcccgc cgttcgggtc tgcaatgctg atactgcttc gcgtcaccct
     1261 gaccacttca atccctgcct ccgtgagcgc ctgaatcaca tcctgacggc cttttatctc
     1321 cccgacatga taaagagcgt ctatcccgcg tgtgacgctc tctgcaagcg cctgtttcgt
     1381 tttcggcagg ttatcaggga gagtcagtat ccggcggttc tccggggcgt tggggtcatg
     1441 cagcccgtaa tggtgattaa ccagcgtctg ccaggcatca attctcggcc tgtctgcccg
     1501 gtcgtagtac ggctggaggc gttttccgct ctgtagctcc atgttcggaa tgacaaaatt
     1561 cagctcaagc cgtcccttgt cctggtgctc cacccacagg atgctgtact gatttttttc
     1621 gagaccgggc atcagtacac gctcaaagct cgccatcact ttttcacgtc ctcccggcgg
     1681 cagctccttc tccgcgaacg acagaacacc tgacgtgtat ttcttcgcaa atggcgtggc
     1741 atcgatgagt tcccggactt cttccggatt accctgaagc accgttgccc cttcccggtt
     1801 acgctccctt cccagcaggt aatcaaccgg accactgcca ccaccttttc ccctggcatg
     1861 aaatttaact atcatcccgc gtcccctgtt ccctgacggc cagacgcagc cggcgcagct
     1921 catccccgat ggccatcagt gcggccacta cctgaacccg gtcaccggaa gaccactgtc
     1981 cgctgttcac cttcctcgct gtctgattca ggttattccc gatggcggcc agctgacgca
     2041 gtaacggcgg tgccagtgtc ggcagcttcc cggaacgggc aaccggctca cccaggcaga
     2101 cccgccgcat ccataccgcc agttgtttac cctcacagcg ttccagtaac cgggcatgtt
     2161 catcatcagt aacccgtatc gtgagcatcc tctcgcgttt catcggtatc attaccccca
     2221 tgaacagaaa tcccccttac acggaggcat cagtgactaa acaggaaaaa accgccctta
     2281 acatggcccg tttcatcaga ggccagacat taacgctgct ggagaaactt aacgaactgg
     2341 acgcagatga acaggcagat atctgtgaat cgcttcacga ccacgccgat gagctttacc
     2401 gcagctgcct cgcacgtttc ggggatgacg gtgaaaacct ctgacacatg cagctcccgg
     2461 aaacggtcac agcttgtctg tgagcggatg ccgggagccg acaagcccgt caggacgcgt
     2521 cagcgggttt tagcgggtgt cggggcgcag ccntgaccca gtcacgtagc gatagcggag
     2581 tgtatactgg cttaaccatg nggcatcagg gcgcattgta tgaaaagtgc accatgccgg
     2641 gtgtgaaatg ccgcacagat gcgtaaggag aaaatgcncg tccaggcgct tttccgcttc
     2701 ctcgctcact gactcgctgc gctcggtcgt tcgactgcgg cgagcggagt ctgctggctc
     2761 aaaaacggcg atacggttat ccacagaatc aggggataag gcagaaaaga acatgtgagc
     2821 aaaaaggcaa agggcaaaaa aagccgcgta gctggcgttt ttccataggc tccgcccccc
     2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3061 acgctgttgg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3181 ggaaagacac gtaaaaacgc cactggcagc agccattggt aaccgggtta gcagagcgag
     3241 atttataagt tggtaacaga gttcttgaag tatcagcctg attctggcta cactagaaga
     3301 acaagttttg gtggtctgcg ctctgcttaa gccagattac ctggttagat aatctgccag
     3361 gattagctaa cctttgaaaa accacctgcc agggtggttt ttctttatgt agagattatg
     3421 cttttaataa gcacgcagtc aaggattaca atctcttata atttaatgta tttttccaga
     3481 tctacattct ctaatgcatc agatataaaa gactgtatgt ctttaagttg aggtttatcc
     3541 tctaaaagat catctacatt cagtttcgaa ctaatatagg aagagacctt tgcaacccaa
     3601 tacggatcac tcatcgcaac aactttacca tcaatctctt cagccatatt aactattcta
     3661 cttctatcac caataacgtc aacaataacc attgtgctta tattatgcat cactccgcca
     3721 atatcatcca ttcgtctaaa tctagatact ttatccaaac ttatttctgt gttaccccta
     3781 ccaataaaac caatgtcaac cctaatcgcc ttcccattaa aaacaagagt agcatcagac
     3841 tccctgtcat cagctcttgt tgaaagagta aaacatttag catttaaccc ttcttcaatt
     3901 ttttctttat attcaaaacc cattattgta aataaagaac cgagtattag cttttcaaaa
     3961 tatttaccat gaagagattt ctcagatccc ctaatagtta aagtttgcga accaagagat
     4021 aaaataatat agcaaaaatc ttctaaagac accttttgcc caccaataga tatctctcca
     4081 attttatctt catacccgtt ttttatttta agtatttctt cttgcgtcct taaagcaagt
     4141 ctatcaaaac catccccatg ctcatctctt aaaacatttt gggtctgttt atttgtcatc
     4201 ataaccatcc actcgagaac aggttgatta gacttaacat aagacttgtt cgataatgca
     4261 ttttttgcat agagataaag ctgctttggc gttatccctt ctttcatttt atccacaaaa
     4321 aagtcaaaaa aagagagatt gctttctaac aatctagtac gagttagtat ttcagtgaat
     4381 tcgcgaacat ttttacctgt taaaacatca taaacaacct ttttaaaaac atccgttccg
     4441 aacttatcta aaacatcatt ttttgaaatt ttcgccaacg ctatgagttc gttagggaaa
     4501 caatttttat ccttgaaatt catatcctca ccatggacat agtaaaaatg agatgctaag
     4561 atagcaggtt gtatggtaac agatcgatag ttaggaaaaa tgacagttaa aaacaattac
     4621 ttaaacagag gttatttggt aacctctacg actcaggcga aaaaaatagc aaagttgcac
     4681 ctagagaaat tagacttagc tgataaattt tcctttgggt tacctgaagt cgatgatcgg
     4741 taccatgtat ggcgtgtacc attagtagcg acaggccaca ggattggtga aatagttata
     4801 aattcaaaaa cgtcaactat tgattataag aaaagttcga agtctccatt tcttattaag
     4861 aaagcaatgg aggttgtcca taccccaaaa aaggtaaaaa aagaaaaaaa aatcatatca
     4921 aagtcaccat taaataacat gttactacaa ggcaactgcg cggagacctt gaaaaaatta
     4981 cctgatgaat cagtgaactt agtattcaca tcaccgccat attacaatgc caaaccagag
     5041 tattccgaat atcatacata tgatgaatac ctctctctat tgcgttcagt aataaaagag
     5101 tgccatagag ttttaagcga aggtcgtttt ttcgtcatca atgtctcgcc cgttttaata
     5161 agaagggcgt cacggaatga agcatcaaaa agaatagctg ttccttttga cttgcacagg
     5221 ttatttatag aagaaggcta tgaattcata gatgatatac attgggttaa accagaagga
     5281 gctggctggg ctttaggtag aggccgtcga ttcgcagctg accgcaatcc gttgcaatat
     5341 aaaccagtac cagtaactga gtacattctt gtttacagaa aaaaaactga taaattaata
     5401 gattggaaca taagaaacca tcatagcaaa gaggatgttt ttgactcaaa aatcggcgac
     5461 gattacgaaa agacaaacct atggaaaata aatccatctc gtaacagaaa acatcctgct
     5521 acatttccat acggtctggc agaaagagtt ataaaatact attctttcaa aaatgatgtt
     5581 attttagatc cttttgctgg atcgggaaca acagcaaaag cagcaattga tttaggcaga
     5641 agatttgtga tgtgtgagat atcaaagcag tatattgatc tgatc
//
