LOCUS       CP077227                2089 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1384 plasmid unnamed2, complete
            sequence.
ACCESSION   CP077227
VERSION     CP077227.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357526
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 657.97x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:56:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,845
            CDSs (with protein)               :: 4,845
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 230
            CDSs (without protein)            :: 230
            Pseudo Genes (ambiguous residues) :: 0 of 230
            Pseudo Genes (frameshifted)       :: 92 of 230
            Pseudo Genes (incomplete)         :: 142 of 230
            Pseudo Genes (internal stop)      :: 28 of 230
            Pseudo Genes (multiple problems)  :: 28 of 230
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1384"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1384"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(join(1451..2089,1..327))
                     /locus_tag="I6L14_25905"
     CDS             complement(join(1451..2089,1..327))
                     /locus_tag="I6L14_25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QXA06190.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            complement(1181..1447)
                     /locus_tag="I6L14_25910"
     CDS             complement(1181..1447)
                     /locus_tag="I6L14_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA06189.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          complement(1792..2023)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tcgggaagac gggtaatcca caacgatttt cggtagagcc tgataaaaac gggcttgcca
       61 catgagggtt cttctccact ggcagacagg gcaatgacgg acacggcaaa attgcgcact
      121 acgaagccgt aggcgcgttt ctccggtttc catgatggta gaccaaccaa aacgtaacaa
      181 accgctacag gaggccatac ggagtgcata gcgctcgaat tcagcagcag tctggtagat
      241 acccccgaca gaatcggtaa gcgtgcgctg ggtatcccaa actgcatcac gggggctgta
      301 gtccgaaagg aatttatctt ctgacattaa gcctgctccg atctgggcag gctgtgattt
      361 gatgtggtat attcatcacg tacctgtgca ttgcgagcca gtacatgtca ccagagtttg
      421 ccgctgaggt gacacattcg aaatgttggg gagtgttgac gcactcccca acgccgatta
      481 aaaacgcctc aattctgctc cattttcttt gacctgtcaa aacccactca aagcctcgtc
      541 attactgggc taaaccacta tttcatatcc gccatttatc ccttaagtat aaatacaagg
      601 atcacgcgcg agaattgcgc aaaaaataac cacaatttta accccccaaa acaagcaccc
      661 ccgaacggct taccccaacc ttttccacac acccgatcac caaaacaggg aataaaaaca
      721 aaaccctgtc ttacaggtgg atggcctcac ttcgtgaaac catccacccc gcataagttt
      781 tttaatcggt ggtgtcgtca atcactaaat cggaacccca aaggggaccc ccacgattta
      841 gcatttgacg agattaactc tcggagatgg catcatgctt ttaacggact ttaaaaaacg
      901 gtgaacggat gcggatttta atccgtgtct gtgaggcgtt gagcaacgag caaagcgagg
      961 cggtagaaag agagccagcg gcaggctcag gacgataatc cggggaaaat gtaccactcg
     1021 cagagtgtta acatttgaca tcggattgtc tatcctgcat tggtgtcgag ttggcaaggc
     1081 tgaggataat gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc
     1141 gtacctatca cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa
     1201 atccaaactt gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg
     1261 ttttctgaat atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg
     1321 tgccaaaact taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg
     1381 cttcacttct taaaaaaaat aaaccaatag tcttatttcc attcaggtat tcaatattaa
     1441 tattcatacg ttaatccgat ttatccttct caggggcgcg tttgtaacgc cgtttacctg
     1501 aatcccagac aaacgccgtc cgcttcccgt catcagtgcc atcccctaca tcgtcggcaa
     1561 tgacaagatc ctcattggtt tctcgatcca actggaggac gttttttagc gccccaccgg
     1621 tcgagataaa acggcgcttg tgaagctgcc gcgtcagctc aagaaaccac tcaggatcgt
     1681 ttgccatatc ttccggttta acggagtatt tcagcgtttc agcaaccgcg ctttgcagtt
     1741 gctcggcaac gttggccaca acctcacctg tcttagtttt tactgcccga atatcgatat
     1801 tcggctcata gttcacccgc aagcaatcgc gccagagttc tacccaacgt tcgtgcttaa
     1861 cgtagttctt ccctttaaac caagaaggtt gcaccatcag cagacagtga aaatgcggat
     1921 gtgcgctgcc atccttacct cgcgtcacct ccgtagccct gatccacccc tgaacaggtg
     1981 atagctcctt tcgcttttcc attcgcttaa acgccgcatt cattgctgta aggactgttc
     2041 caagttcacc tatctcgcag ttcctgacag ttaacgtcag aaacaacca
//
