LOCUS       CP077219                1549 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1379 plasmid unnamed4, complete
            sequence.
ACCESSION   CP077219
VERSION     CP077219.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357521
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 972.28x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:24:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,883
            CDSs (total)                      :: 4,761
            Genes (coding)                    :: 4,617
            CDSs (with protein)               :: 4,617
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 64 of 144
            Pseudo Genes (incomplete)         :: 71 of 144
            Pseudo Genes (internal stop)      :: 39 of 144
            Pseudo Genes (multiple problems)  :: 25 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1379"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1379"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            363..488
                     /locus_tag="I6L09_24390"
     CDS             363..488
                     /locus_tag="I6L09_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="QXA01361.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
                     /name="replication protein"
     gene            complement(933..1409)
                     /locus_tag="I6L09_24395"
     CDS             complement(933..1409)
                     /locus_tag="I6L09_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QXA01360.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(244..342)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          442..702
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="8.62e-98"
ORIGIN
        1 aaaaaacaca ctaaaaaaac aaccacttac aaatcggcgt aaaagatcta ttctaggcaa
       61 gggtgttgcg tgcaacgcaa cgccctttgg gggtgtcggg gcgaagccct gacccagtca
      121 cgtagcgata gcggagtgta tactggctta ctcctatgca agcctcgcca gccgtgattt
      181 ttgccagatt tcgtggtttt cacgttcaac agctccatcg tgtttttgcc gtccgttcac
      241 cgttaaaacg cctcagaaat cgggacagga tgtgtaatcg tataaccgcg catgcacgcc
      301 gatacgatta ccacttggtc agggcttcac cccgacaccc cgcatcagtg cgtaagtgct
      361 caatgtggct tccggttctc ttccgcttcc tcgtcgattc gctcgctacg ctcggtcatc
      421 gacttgcggc gagcgggatc acctctcgca tgttgtcggt gatcccggtg ttgttctttt
      481 ggggatgagt cgccagatag cccctcaaac gcgctcaaaa gctctcagac gcacgaaacg
      541 ggttttagat gcccgccagg gcatggaagg cgcgaaaaac gcttacagag gctctcagga
      601 ggtcaagcgc gccgataccc ctaaccaggg gctttcaggt cgccaggagc cgcgccagcc
      661 ccctgcaaag cccatttgca cggaacaggc agccatgcgg ttggtaggtg gtcgtagacc
      721 gtcggtgttg cagcgatggg ggtctggggt tctccccaga cggtttccgg tagcgctcaa
      781 ggcgctgccg gaaccgtggc ggctcggttt tttctggaga acggggagca aaaacacgca
      841 aaggattttg aggcggcaaa agagcaatgc aggatagaca atcatggtca gaacgttaac
      901 acgtagcccc tgattgtccg cctgccgcta gctcaatttg aaaacatggc catgattcgg
      961 cgctgaatat caccgtcttt gttgaccagt gttttagtga caaacgctgc atggtttttc
     1021 gtagtgttgt atcccttcca gacttcatgt ggatcgaggg catacgcaca aaccttacca
     1081 gcaacgggga ttctaactaa tgcatttttt gattctaagt aattgagcca ccgaataatt
     1141 gtgcttctgc taactccaag tttttcagct aggaattgct gatccgcaac gacagcgcca
     1201 cacgtaggat cgatatgctc cgctaaaaat gagtacagct tcagtgctga tatgccttgg
     1261 ctatccttca gcagctctcg aacacgttcc caaccttttg gggatacctg tgtaaacctt
     1321 gaatttttct ttgcttcttc gagatcctcg gtgtttttct ttgcctcatc tgaaagttgt
     1381 ttcagtcgtt tttctgaatc aataaacatg attttttccc tctgaaaatc ccatgcacta
     1441 acgttggttc aaatctaagc catcatgaca catctgtgca acaccttgtt gcgtgttgtc
     1501 gcatcctgga tgcgacaacg ttgtgtcatc caggatgcga caggtaaat
//
