LOCUS       CP077218                7938 bp    DNA     circular BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1379 plasmid unnamed3, complete
            sequence.
ACCESSION   CP077218
VERSION     CP077218.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357521
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 972.28x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:24:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,883
            CDSs (total)                      :: 4,761
            Genes (coding)                    :: 4,617
            CDSs (with protein)               :: 4,617
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 64 of 144
            Pseudo Genes (incomplete)         :: 71 of 144
            Pseudo Genes (internal stop)      :: 39 of 144
            Pseudo Genes (multiple problems)  :: 25 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1379"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1379"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            10..210
                     /locus_tag="I6L09_24340"
     CDS             10..210
                     /locus_tag="I6L09_24340"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA01355.1"
                     /translation="MRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAF
                     IENWIKVNGGWKMQFISYEKI"
                     /name="hypothetical protein"
     gene            complement(235..1128)
                     /locus_tag="I6L09_24345"
     CDS             complement(235..1128)
                     /locus_tag="I6L09_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="QXA01356.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(1131..2723)
                     /locus_tag="I6L09_24350"
     CDS             complement(1131..2723)
                     /locus_tag="I6L09_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="QXA01357.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein/permease"
     gene            complement(2762..3460)
                     /locus_tag="I6L09_24355"
     CDS             complement(2762..3460)
                     /locus_tag="I6L09_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QXA01358.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEE
                     ADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAY
                     LHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVR
                     YSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKG
                     CGAGCSCNSR"
                     /name="hypothetical protein"
     gene            complement(4825..5016)
                     /locus_tag="I6L09_24360"
     CDS             complement(4825..5016)
                     /locus_tag="I6L09_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QXA01351.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5081..5404
                     /locus_tag="I6L09_24365"
     CDS             5081..5404
                     /locus_tag="I6L09_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QXA01359.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVVRSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5394..6104
                     /locus_tag="I6L09_24370"
                     /pseudo=""
     CDS             5394..6104
                     /locus_tag="I6L09_24370"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6082..6600
                     /locus_tag="I6L09_24375"
     CDS             6082..6600
                     /locus_tag="I6L09_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QXA01352.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            6607..6840
                     /locus_tag="I6L09_24380"
     CDS             6607..6840
                     /locus_tag="I6L09_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QXA01353.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            7551..7790
                     /locus_tag="I6L09_24385"
     CDS             7551..7790
                     /locus_tag="I6L09_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="QXA01354.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 domain-containing protein"
     Typing          4670..4749
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.78"
                     /coverage="92.0"
                     /evalue="8.94e-33"
     Typing          complement(4095..4637)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.679"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          5052..5398
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="98.0"
                     /evalue="3.77e-66"
     Typing          5395..6944
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagattgata tgcgtccagc gaggatttta cctagcatag aaagatatga atatgatgtc
       61 gtaatgcgtg agtttaaagg cattgcttgt gtttcaggga aagctgaaat aaagcaaaat
      121 gatggaaata tattaatggc ttttattgag aattggataa aggttaatgg tggatggaaa
      181 atgcagttta tatcgtatga aaaaatctaa ggggaagccc ccttagatta atggttatat
      241 atgttcaagt tttatttttt tatagcgata agttctgctt tgagctttaa gcatgttttc
      301 tgcaaactga atatcaaaac tatttatagc attagttatt atagttttta ttttttcttt
      361 atcagataat gaatcaccat gctctatagg ttcccgcata tatggattgt tttttaattc
      421 atcaaataat gttttaaata gttctaatcg acgaggtctt ataactgtaa aagatatatg
      481 aagagagtgt ttgctctgag tcgtaacatt gtgccagtat ccagcaggaa gatatatggc
      541 ctgacctggg gttatatcaa actcataatc tggggaggta ttaggtggta gataatcgaa
      601 gcttctttca tcttcgagag ggtatgaata ggtagggttg taaactttcc atttttttat
      661 tccctcaatt tgaacagcta ttacatcatg attgtcaaaa tgaagcccaa agcttggtgt
      721 tgctgtaaat gcagcatata gattagcact acatgtacat tctaactctt tagataacca
      781 tagtctgctt tcatctacag attcaaagaa tgactggcat cgatctacta taagggttgc
      841 gccatcacgt aagcatttat atagctgatg gcgatttata tgtggtgttc tatcgcctgt
      901 ttggctatat gtgtatctta taaatccttt atacccccta atttcgggaa agttgtcatt
      961 tgccattcta actctaggat agtgcaatat atctttttca agcaatttat taagatcatt
     1021 ccagttgatt aagtttttat aattaacatc cgtgaataca aacggatatt tctggaaatt
     1081 atatttctta aatttttcta tgatttcctt ttttataaga ttattcattt ttaaacctca
     1141 tgttttgtga tatctataat ctgtgcttta ggtatattat ttataagttg ttcatcatga
     1201 gatataacaa tgatatgctt ttccttgttt tcctctttta tataatcact gattattttt
     1261 ataccaagtg aatcaaaccc ttttgtcggt tcatcgaaaa taatcaagtc tacgtttttt
     1321 ccagatgtaa gagatactcc taatttttgt ttttgtccac cagataaatt atgtccgtaa
     1381 cctttgatct cataatctag tggtttgtcg aacttaaatc tgtgtagcag ctttttaatt
     1441 gtatttaaat tattaatgcc ggaataatgt aatatatttt cctctatagt tccaggcatg
     1501 agatctagat cttgaggaac gtataagaca ttaacaaaag gagaatttgc tttttcttgt
     1561 tgtatcccat tgatttttat tgtgttttct tctgttgtta tattgccaga gagaattttt
     1621 gcaatagttg tttttcccga accattctca ccggttataa aggtaacaac gccttttgtt
     1681 aatattaatt tatggttgaa tgatttgttg tatatttcaa tattgcaaat attgtctttt
     1741 acatgaagat attgcttgtc tgtgtaattt gtttttttta tgtcgtttat ttcggcacag
     1801 tataaatcaa tttctgttct tgccattttt atttgtctaa atagataacc aatgcgattt
     1861 aatgggagaa aaacttggaa taaaaaagtg gcaagcataa taatactacc acggcttgct
     1921 ctaccatgaa gaaacatcca gtatgctgat aggataatca gactactaaa aatgactgaa
     1981 agaaaagcgg agctgatacc aaacaggaaa aattcattat ttacttttct ttgtttttct
     2041 gaatgtgtat ttacagcttg aattactcgt ttacgacttt tatccgtggt atggaaagct
     2101 ttatccatag gatacatgtg catcatatca aacattcttc cagatgcgaa tttggagcta
     2161 agtgcaactg atttcatcat tggaaatcta cgttgtgtca tttttatgac aaatattata
     2221 tagattgcca ttattattat aaactcaatg gctattaatg tattaacact tatacaaatt
     2281 gttatgatta ttccaatcat ttgtattaat aatggtacta catcagaaca tattgtataa
     2341 aataatgatg atgcagaaga aagcatgctt tctataacct tatttagttc tcctttattt
     2401 ttctttaaaa aatgctcttg gtgagaattt tgtatattat caataacttg ctctctaagc
     2461 tttacagata attctatatt tgttttttga gtcaagtatt ccgatatgct atatccagca
     2521 ggcataagga atctaattga taagaaacat gctataaata ttaataatga gtttatttct
     2581 cctattgaat ctttattgtc aataactgtt tttattaaat atccgaatag aacgaaaaga
     2641 aaactactta tagttgataa taatataata aaaaacaaat tacatttatt aaatgtgatg
     2701 atgtactttt caataaaatt caatttataa tccttaaagc ccgtatatta cgggctttat
     2761 gttatctaga attacatgag cacccagcgc cacatccttt accgctgcaa ccacaaacag
     2821 aaccattatc attccaaaca tcttttcgca ttattatttt tacaaattct gcggctggaa
     2881 ggtgagtgat actctttact ttttcgatgt aagaatcact ggatatggat tcttttggaa
     2941 ttcttatagt attaatgttc tttatgctgt atcttacagc ataatcacca taccactttg
     3001 cgacagtttc atgacctagg taaaaagcga ttttttcaat ttgattttta tattcgttaa
     3061 aacgatattc ttggaatgtt ataccatgct cattaacatg ccttatctca ttttttagca
     3121 tgtcatttgt atttggataa tggtggaggt aagcaccaag atatttttca caaaagctgg
     3181 tataagcatc tgtaaattga ataaacgtat gccacatttc atctataatt tgtaattgct
     3241 cgtgaacttt tatattgtag cattccgtta ggcatgttga ggctagccag atgaatttct
     3301 ttgtttcatt aaagatatca tcagcttctt ctggtgttac attccattct ttacgaaatt
     3361 ttagaagaac atcatcattg tgataactca tgatatcttc taatgagcta acattatgaa
     3421 cttttgcata agaactgctg ttcatcttat gttgtagcat aacaccccca attatatatt
     3481 tgaatgtaga tgagaatagt tatcatttgt tatggtattg ttaaatttgt gcttgtgtca
     3541 atatgcctaa ttctataaat gagatagtaa tcaagttttt taatggttat aggatccatt
     3601 agtaatacaa ggtttttgca gccagttttt acgggtaggg gctgcaagga aaacaaaatt
     3661 ttcccactct aagagaagag aattgagttg gttgaatgag tggatgcatc tagggccgct
     3721 aattcaccga gtgtaaaggc tgtacgaaat gtgttgcagt ccgataatag ccccctccat
     3781 tgacgtttta cacatgtgtt gtaggttcca ggagtgggcg gatctcaaaa agatcaaagg
     3841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc cagtaaacga aaaaaccgcc
     3901 tggggaggcg gtttagtcga aggttaagtc agttggggac tgcttaaccg ggtaactggc
     3961 ttggtggagc acagatacca aatactgtcc ttctagtgta gccgcagtta ggccaccact
     4021 tcaagaactc ttaatatctc aatccacctt gtccagttac cagtggctgc tgccagtggc
     4081 gctttgtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4141 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgag
     4201 ccgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4261 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4321 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4381 tttttatgat gctcgtcagg ggggcggagc ctatggaaaa acgccaacgg cgcagccttt
     4441 tcctggttct cgttttttgc tcacatgttt cttttggcgt tatcccctga ttctgtggat
     4501 aaccgcatct ccgcttttga gtgagcagac accgctcgcc gcagccgaac gaccgagtgt
     4561 agcgagtcag tgagcgagga agcggaagag cgccggaacg tgcattttct ccttacgcat
     4621 ctgtgcggca tttcacatcg gacatggtgc gctttccata caattcgtac tgatgccgca
     4681 tagttaagcc agtatacact ccgctatcgc tacgtgactg gttcagggct tcgccccgaa
     4741 accccctgac gcgccctgag gggcttgtct gctcccggca tccgctcaca gacaagctgt
     4801 taccgtctcc gggagctgta tgtgtcagag gttttcaccg tcatccccga agcgtgcgag
     4861 gcagctgcgg taaagctcgt cggcgtggtc gtgaagtgat tcacaaatat cggcctgttc
     4921 atctgcgtcc agttcgttga gtttctccag cagcgttaat gtctggcttc tgataaaacg
     4981 ggccatgtta agggcggttt tttcctgttt agtcactgat gcctccgtgt aagggggatt
     5041 tctgttttat ggggtaatga taccgatgaa acgcgagagg atgctcacca tacgggttac
     5101 tgatgatgaa catgcccggt tactggaacg ctgtgagggt aaacagctgg cggtatggat
     5161 gcggcgggtc tgcctgggtg agccggttgt acgctccggg aagctgccga cactggcacc
     5221 gccgttactg cgtcagctgg ccgccatcgg gaataacctg aatcagacag cgaggaaggt
     5281 gaacagcggg cagtggtctt ccggtgatcg ggttcaggtg ttggctgcgc tgatggccat
     5341 cggggaggag ctgcgtcggc tgcgtctggc cgtcagggaa cagggcgcgc gggatgattg
     5401 ttaaatttca tgccagggga aaaggcggtg gcagtggtcc ggttgattac ctgctgggaa
     5461 gggagcgaaa ccgggaaggg gcaacggtgc ttcagggtaa tccggaagaa gtccgggaac
     5521 tcatcgatgc cacgccattt gcgaagaaat acacgtcagg tgttctgtcg ttcgcggaga
     5581 aggagctgcc gccgggagga cgtgaaaagg tgatggcgag ctttgagcgt gtactgatgc
     5641 ccggtctcga aaagaatcag tacagcatcc tgtgggtgga gcaccaggac aagggacggc
     5701 ttgagctgaa ttttgtcatt ccgaacatgg agctacagac cggaaaacgc ctccagccgt
     5761 actacgaccg ggcagacagg ccgagaattg atgcctggca gacgctggtg aatcaccatt
     5821 acgggctgca tgaccccaac gccccggaga accgccggat actgactctc cctgataacc
     5881 tgccggaaac gaaacaggcg cttgcagaga gcgtcacacg cgggatagac gctctttatc
     5941 atgtcgggga gataaaaggc cgtcaggatg tgattcaggc gctcacagag gcagggcttg
     6001 aagtggtcag ggtgatgcga agcagtatca gcattgcaga cccgaacggt gggaagaata
     6061 tcaggctgaa aggagcattt tatgagcaat cttttacaga cgggcgcgga gttcgagaaa
     6121 aagctgaaag agagagccga atctacagag acaatgctga acgacgagtt cagcaagctc
     6181 gaaaaatctg taagcaaggc tgtgacatca aacgagacga aaatcaaaga cgctatagcc
     6241 ctgttcacag cttcgacaga ggaatcactg aaaaaacacc gggaaggggt gaaagaggcg
     6301 atgatgcagc acaggaagga cgtgttaaag ctggcaggga atacgggcat gatgttactg
     6361 gggatggtcc ttttcctgtt taccgtgagt ggcgggacgc tctggtatct tggcgggatg
     6421 atacaggcga acctggaaga aatcaggata caggaagaaa cattgcagaa actgaacgcg
     6481 aagacatggg gcgtggagtt tgtgcaggac gggagaagga aatttctggt tatccctcag
     6541 gggaaatcgg caacggtgat tccctatcag gggaaggact gggtacaact gacggagtga
     6601 cacagagtga cggagctgga aagacatttg ctgaacgcct tagagcaact gcaacaggac
     6661 tatatgcagc ggctgaacga atgggagagc gccttcgtgg aattgcagaa aatgttttcg
     6721 cttacgcaac gggacaacgc gatgctgaac gagcgggtca tgcagttgag tcagcaggtg
     6781 cagcacttga gcgagcagac agaacgcttg agccagttat acagcgagaa caggagataa
     6841 gggatgaacg gctgatacag gaacgcgaac atgagttatc cctggagcgt gagcgccagc
     6901 tggaaataca ggaacggaca caggatggcc cttcgctggg atggtaatca gagacaggct
     6961 aaaatgttgt cagcgcgttt ccggtgcatt ttttgggata atggttggtt cgggggcacc
     7021 gggaacgcgc ttcagtcaga ccttgctgaa aacacaggaa cgcgttaaaa cgcatacggg
     7081 cgcgattctg gtgtgtcttt tgttgctgtg ttttgcctct attgccagat cgcgctcatt
     7141 cccctgaact tctgttttcc cgctccggtc cccgtcttct tcgcttccga aagcatgaaa
     7201 aaaatccgtt tcagctgtct ctggctggca attcctgcgt gattcacatg gctgcatagc
     7261 tatgcatgtg gttaaaattt accgggtgcg atcgcggcag tttttcgggt ggtttgttgc
     7321 catttttacc tgtctgctgc cgtgatcgcg ccgaacgcgt ttcagcggtg cgtacaataa
     7381 gggattatgg taaattatct tgttccttag agaatgattt gaggtggtat cctgcactta
     7441 tttaaatatg cttgcttggg aaattccagg ttgcagtatg gttgagttgt aattttccta
     7501 tgataggaaa aatgttatat tattaattta tttgtgaggc ttcataaaga atgaaaattt
     7561 ctgatattga aaaaaacata tataaaggac tagctgaatt tatttcacaa aaaatcagag
     7621 atgaacatga tatatgcctt ggacagtttg aatcagagga attacttgat gagattctta
     7681 aaagagtaat cccagtcata tataatgtgg ctattgatga tgctataaaa tcagtaagga
     7741 atgttgggga tagattagag gaggaattag atatgaggaa ggtagtgtga ctaattttaa
     7801 atcagactta aatctaataa gaaaagaatg gtataatgca ttttattcag gtaatactga
     7861 aatgctagat tacttggaag ctgattggtt tatttcaaca aatggtcaaa aagttatcta
     7921 taaaaaacat cagttacg
//
