LOCUS       CP077217                9844 bp    DNA     linear   BCT 28-JUN-2021
DEFINITION  Escherichia coli strain FDAARGOS_1379 plasmid unnamed2.
ACCESSION   CP077217
VERSION     CP077217.1
DBLINK      BioProject: PRJNA231221
            BioSample: SAMN16357521
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Walk,S., Carlson,P., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J.,
            Ott,S., Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J.,
            Nadendla,S. and Yan,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, White
            Oak, MD 20903, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 9.0.0, HGAP v. 4.0, SPAdes v. 3.14.1
            Genome Coverage                   :: 972.28x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 16:24:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,883
            CDSs (total)                      :: 4,761
            Genes (coding)                    :: 4,617
            CDSs (with protein)               :: 4,617
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 64 of 144
            Pseudo Genes (incomplete)         :: 71 of 144
            Pseudo Genes (internal stop)      :: 39 of 144
            Pseudo Genes (multiple problems)  :: 25 of 144
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1379"
                     /isolation_source="Seth Lab isolate"
                     /host="Seth Lab strain"
                     /culture_collection="FDA:FDAARGOS_1379"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: MT"
                     /collected_by="Montana State U"
     gene            complement(1..412)
                     /locus_tag="I6L09_24290"
                     /pseudo=""
     CDS             complement(1..412)
                     /locus_tag="I6L09_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370035.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /name="head completion/stabilization protein"
     gene            complement(601..1076)
                     /locus_tag="I6L09_24295"
                     /pseudo=""
     CDS             complement(601..1076)
                     /locus_tag="I6L09_24295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /name="terminase"
     gene            1271..2811
                     /gene="istA"
                     /locus_tag="I6L09_24300"
                     /pseudo=""
     CDS             1271..2811
                     /gene="istA"
                     /locus_tag="I6L09_24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /name="istA"
     gene            2826..3572
                     /gene="istB"
                     /locus_tag="I6L09_24305"
                     /pseudo=""
     CDS             2826..3572
                     /gene="istB"
                     /locus_tag="I6L09_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /name="istB"
     gene            3684..3887
                     /locus_tag="I6L09_24310"
     CDS             3684..3887
                     /locus_tag="I6L09_24310"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="QXA01350.1"
                     /translation="MSISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLD
                     AAAEQPESLAQRTRKPSVSAVR"
                     /name="transposase"
     gene            3966..4313
                     /gene="tnpB"
                     /locus_tag="I6L09_24315"
     CDS             3966..4313
                     /gene="tnpB"
                     /locus_tag="I6L09_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="QXA01349.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVFI
                     FRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDWRQ
                     PKRLLTSLTML"
                     /name="tnpB"
     gene            4362..5897
                     /locus_tag="I6L09_24320"
                     /pseudo=""
     CDS             4362..5897
                     /locus_tag="I6L09_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /name="IS66 family transposase"
     gene            complement(5934..6116)
                     /locus_tag="I6L09_24325"
                     /pseudo=""
     CDS             complement(5934..6116)
                     /locus_tag="I6L09_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /name="IS66 family transposase"
     gene            complement(6146..6494)
                     /gene="tnpB"
                     /locus_tag="I6L09_24330"
                     /pseudo=""
     CDS             complement(6146..6494)
                     /gene="tnpB"
                     /locus_tag="I6L09_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /name="tnpB"
     gene            6995..7510
                     /locus_tag="I6L09_24335"
                     /pseudo=""
     CDS             6995..7510
                     /locus_tag="I6L09_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
ORIGIN
        1 ctcacgatgc agcgcggctt gtcctggata cgcgccaccg cccagcgcat atcccgccac
       61 agctcatcaa tggtgctgtc aatgctgtca gccttcttgt cgcctttcgc actggcatcc
      121 acgccgcgat aacgctcata aagcgacgct ggtcgccatc gcacacacgg cgcgctcgta
      181 gtaaaaaact ttgatgcttt caccgtcgat atcgtccgcc gggacgtccg ccagacgcgt
      241 aaaaaccggc ggcaattttc tgttcgcggt actcgtacag ctccgcattc gtctccgcca
      301 tgcctgactt gatggcctca cgcagacgag cgggggctac ggtctgctca aggcgcatac
      361 gttcccggac gcgcttcggg tcgatatcgg ggaaaaagaa cgtgttttta atcaccggct
      421 cgtcgctgcc ggttgcggga tgaccaccgt accctcaccg gacacgggag cctcctttcg
      481 cggaataatc agcgtcatca tgactacctc tgaaaagtcg ggcggtggac gccggtgcag
      541 tgtcaggtga ttcaccctca ctgaccggcg tgccgccctg gcgcggggcg cattcggttg
      601 ttaactggct ttctttttcg ggcgtccacg ttttgccggt gtcacgtccg cggtcttacg
      661 gcggggtacg ggtggccgct tttggctgcg gctccggctt cggtttcagc tcccgctcca
      721 gtcgttcaat ctctttttga cgcctgcctg acagtcgagc tgtgtcgcac gttgcaggtg
      781 cgccagcgcc cctgcggcat caccagcgtc acgcagaaca gaccggtgat tttgtgcagc
      841 tttgcgcgca ttcatcaggc atgtctgccg tggcagtcag ttcaagtgtg tccgtcagca
      901 ggcgggggat ccacagactc accggcagcg cgtgagcgcg catggcccgc gagcgccacc
      961 tcctcggtga acatgtacgg cggggtgcgg cggtgtttta ccggcatggt cagaccgtac
     1021 ttcagggcgt aacgggcaat ctccagcgcg ccggcaatat caccggtatc cagacgctgc
     1081 gtattttcat gaaaggagat cactcaataa cttccatcga gatcgggtaa taacatttga
     1141 acagatcgct gaataacatc gatggagatc acttttgact cattttgtta ttcagtgatc
     1201 tccatcaatg ttattggaac ttcacaggtg tgttgatctg tatcttttgc cattccggta
     1261 aaggatacct atgccaacag ttccaatttc tatgagaaaa cttaaagaaa ttcttaggct
     1321 taaatacggt gttggactca gccatcgaca aattggtcgt agtcttgcaa tctccccttc
     1381 cgttgtatcc agatatgcta atcgggcggc tcaacttggc ataaagcagt ggcccttacc
     1441 tacaggatgg gatgatacaa aactaaaaca tgcgttcctt cagacccagg ttaagatgaa
     1501 gaagcactct ctgcctgact gggctacagt acaccgggaa ctgcgtaata aatgcgtgac
     1561 gctgcagcta ctctgggaag aatactgtga gcgtaatcca ggcggttttt acagctataa
     1621 ccattactgc cggatgtacc gtgaatggct caaaaccact tcaccatcaa tgcgtcaggt
     1681 acataaagct ggcgaaaaac ttttcgttga ttactgtgga cctaccgttg gcgttaccga
     1741 ccctgagacc ggagaaataa gaactgctca ggtcatcgta gctgttctcg gggcatcaag
     1801 ttacacatgg gcagaggcca cctggtctca gcagcttgaa gactgggtga tgagtcatgt
     1861 tcgctgcttc cagtggttgg gtggcgttcc tgaacttgtt gttccggaca atctgaaaag
     1921 cgccacatcc agggcatgta agtatgatcc tgacgttaac cctacctacc agcagatgct
     1981 tgagcattat aatgtcgcag tttgcctgcg cggccacgta aaccgaaaga taaagccaaa
     2041 gctgaagttg gcgttcaggt tgttgaacgc tggatcatgg cccgaatcag gcatgagatc
     2101 ttctacagcc ttgcatcgct taatcagcgc attcgggagt tgctggaaaa gactgaataa
     2161 caaaataatg cagaagttgg gttattcacg tgcagaactc ttcatccagc ttgataaacc
     2221 cgcactgaag cctcttcctg aagccagtta cagttacacc ctgtgaagaa agtcagagtt
     2281 catgccgatt accacgtgga aatcgacaaa cattactact cggttccatg ttcgctgtta
     2341 ggccagcaac tggaagcatg gatctccgga gaactggtaa gactcttcaa tcaggggcag
     2401 gaggttgctg tgcacccgcg caagcgtact tatggctaca gtacccgcaa cgagcacatg
     2461 cctgaagctc atcgacagca tgccacctgg acgccagagc gtcttctgga atgggcgggg
     2521 cacataggca gtgaaactca tagttatgtg cttcatatac tgaactctcg tccacatccg
     2581 gaacaaagct atcgcttctg ccttggactc ctgaaccttc ataaaaaata cagtaaagcc
     2641 agacttaatg cagcatgtgc aagagctctg aaaacaaagg tatggcgtct gtcaggtatt
     2701 aaatcgatcc tggaaaaagg tctggataaa caacctgttc aggatccaaa accagatctg
     2761 ttatccacga tggaacacga aaacgtacgc ggcagtgagt attaccactg atacgggatc
     2821 caatgatgaa tcatctttac gaacaactga ccgcacttaa actcaccggc ttccgtgatg
     2881 cgcttaaaaa gcaacttgct cagccgggca cataccagga gctgggcttc gaagaacgcc
     2941 tgtcattact gacagcagaa gaactaacct gccgtgaaaa caggaggcag agcgtctgat
     3001 caaacatgca cggttcagac ttaatgctga gttatcaaag ctggattatc gtaacaatag
     3061 agggctggac agggccctca tccgttcact cagtcaggga aactggttaa ccctgaaaca
     3121 aaaatatttt actgaccggg gccaccggca gcggtaaaac gttcctggca tgtgcacttg
     3181 gtcataatgc ctgccgacag ggatacaagg tctactatta tcgccttaaa gcgctgatgg
     3241 aacagtgcta tcaggggcat gctgatggaa gatacagcaa acttttgacc aggctgaata
     3301 atagcgatct gctgcttctg gatgactggg ggctggaacc tctctcatca gaacagcgta
     3361 gcgacctgct ggaaatagtg gatctgatgt accaacgagg ctcaatcatc gtagtgagcc
     3421 agttgccggt ggaaaactgg tacaaaatga tcggagactc cacacatgcg gatgccatcc
     3481 tagatcgact ggttcatggc agtatcaaga tcgaacttaa aggagaatca atgcggaaaa
     3541 tacaatctcc gttgaccgaa ggagatcagt gaaggtaatt aaaaacggtt ctgtgaaagt
     3601 gacacgaacc gatctccatc gatgttactc accgatctcc ttcacggtaa tacgcagctc
     3661 gttgctgcct cctgtgaacc gggatatcca tctcaaaact tgctcttgaa aatggcatta
     3721 acgccaatct gttgttcaaa tggcgacaac aatggcgcga gggaaagctg ctattacctt
     3781 cttcagagag cccccagcta cttcctgtga ctctcgatgc agctgccgaa cagccagaat
     3841 cgctcgcgca gaggacccga aaaccctcag tatcagctgt gaggtaacgt tccggcacgg
     3901 gacgctccgc ttcaatggca atgtcagcga aaagctcctg actctgtgat acaggaactg
     3961 aagcgatgat cccgttacct tccgggacca aaatttggct ggttgccggt atcaccgata
     4021 tgagaaatgg cttcaacggc ctggcggcaa aggtgcagac gacgctgaaa gacgatccga
     4081 tgtcaggtca cgtttttatc ttccgtgggc gtaatggcag tcaggtaaag ctcctctggt
     4141 ctaccggcga tggactgtgt ctgctgacca aacggctgga gcgcggccgc ttcgcctggc
     4201 cgtcagcccg ggatggcaaa gtgttcctca caccggcaca gctggcgatg ctccttgaag
     4261 gtatcgactg gcggcagcct aaaagactgc ttacgtccct gactatgttg taagcctctt
     4321 tatcctggtc gacgctgaat gagcctggta atatacccgg tatgagcagc tcacttcctg
     4381 acgatatcaa tgcactgaaa cgtctccttg ccgaacagga ggcgctgaac cgtgccctgc
     4441 tggaaaagct gaacgagcgt gaacgcgaaa tagaccatct gcaggcacag ctggataagc
     4501 tgcgccggat gaacttcggc agccgctccg aaaaagtctc ccgtcgtatc gcacagatgg
     4561 aagctgacct gaaggcactt cagaaagaaa gtgataccct taccggtcgg gttgacgacc
     4621 cggccgtgca gcgcccgctg cgtcaaaccc gcacccgcaa accgttcccc gaatcactcc
     4681 ccgcgatgaa aaacggctgc tgccggcagc gtcatgctgc ccggaatgtg gaggctcact
     4741 gagctatctg ggtgaggatg ccgccgaaca gctggagttg atgcgcagcg tcttccgggt
     4801 tatccggact gtacgtgaaa agcatgcctg tactcagtgc gatgccatcg tgcaggcccc
     4861 cgcgccttca cggcccatcg agtggggtat cgcaggaccg gggctgctgg cccgcgtgct
     4921 gatctcaaag tatgcagagc acaccccgct gtaccgccag tctgaaatgt acggccgcca
     4981 ggcgtggagc tgagtcgttc actgctgtcg ggctgggtgg atgcatgctg ccggctactg
     5041 tcaccgctgg aagaagcgct tcaggactat gtgctgactg acggtaagct ccatgctgat
     5101 gacacgcctg tcccgtgctg ttgccaggca ataagaaaac gaagaccggg cggttatgga
     5161 cctacgttcg tgacgaccgt aacgccgggt caacgctggc gccggcggtg tggttcgctt
     5221 acagcccgga cagaaaaggc atccatccgc agacccatct tgcggggttc agtggtgtac
     5281 tgcaggcgga tgcatacgcc gggttcaacg agctgtaccg ggatggccgg ataacggaag
     5341 ccgcctgttg ggctcacgcc cgccgtaaaa tccacgatgt gcacgttcgc accccgtcag
     5401 ccctgacgga ggaagcgctg aaacggatcg gcgaactgta cgccatcgag gcagagataa
     5461 ggggaatgac ggcggagcag cgccttgccg aacgtcagtt gaaaacgaaa ccgctgctga
     5521 aatccctgga aagctggctg cgtgaaaaga tgaaaaccct gtcgcgacac tcagaactgg
     5581 cgaaagcgtt cgcatacgcc ctgaaccagt ggccggcgct gacgtactat gcagatgatg
     5641 gctgggctga ggcggacaat aacatcgctg aaaatgcgtt gcggatggtc agtctgggcc
     5701 gcaaaaacta cctgttcttc ggttcggatc atggaggaga gcggggagcg ctgctgtaca
     5761 gcctgatcgg gacgtgcaaa ctgaacggag tggagccaga aagctacctc cgctatgtcc
     5821 ttgacgtcat agccgactgg ccgataaacc gggtcggcga actgctcccc tggcgcgtag
     5881 cactgccgac tgaataacac atccccgtca atacggttct tgctgcacgc ttacagatgt
     5941 tctatttccc gttcatcttc ttcgatcttt tcttcggcac gtgtcagtgc agagcgcagg
     6001 aaggcttccg tctcttcaac cagactcagt tgctggtctt tctgacggag ggcttcagcc
     6061 tgctcagaga gcaatctttc cagctctgcg atgcgaatga ggtatttctg actcatgacc
     6121 gttttataat gcggtcagga gttttttaca acattgtcag tgagttacgg ctggatgttt
     6181 ttggctgacg ccagtccagc ttatcgagga gcattgccag ttgcgagcgg gtaatggata
     6241 ccttgccgtc acgtaccgca ggccagataa actggccttc ctcaggcgtt tggtgaacag
     6301 gcacagacca tcagcatcag cccaaagaat ttgacggtgt caccccgtcg gccacggaag
     6361 ataaacaggt gaccggagaa gggattatca ttcagcacat gttgtacctg ttctcccagt
     6421 ccgttgaagg atttacgcat atcggtaacg ccggcaacga gccagatacg ggtacctgat
     6481 gggagtgaga tcatcttccc ctccggtcag ttcacggatc aacaccgtga gcagctctgg
     6541 cgatggattt tccagcgtca tgttaccgtg acggaattcc accttgcagg aactggcact
     6601 gactctggtc tgagtggaat ggataaagac ggcgcaatgg ccgccacagg tttttctgct
     6661 cacggtcgta tttctacagg taataattca acgcagtgtc agaagaggtc gttaccggaa
     6721 gacgccgcga aacacgcctt cgttctgcca gagcctgagc catttgaaaa taacattcat
     6781 tgacgccatt ttcacgtgca atctgtgcaa cacacaagct ccaggttgtg atgccagttc
     6841 acaccatacg aagtttgaat tcattcgaat agtttttacg aggttctttt cgccagtcct
     6901 gtaattccat acttagatgt cgtctatatc agatgggcgt ctaagttacc aattctcgtc
     6961 tgatggctac atacggcggt cagtttacgc ttacgataaa acccaccaga atgaacatct
     7021 gcattttact gttgaaaatc gtcgtcaggc aaagggaatt cgcttcctgc ggcagtacag
     7081 tacagcgata agtggatgac tgaatcctgg caggaaagct gatatggttg agtgcgcaga
     7141 tgaagcggac gagccagcgc tgagtgctga aagaactggc aaaggatatc tggagcagac
     7201 catggagcga ggaaaggtgc atggctgggc tggcgggtta acagtgacgt ctccatgatg
     7261 aagaaacgca gctaaaatga tagggaaaag gttgtacggt atccgaacgc ctggtgccat
     7321 ggtgtatcaa acggaaatgc agaagcactg aacagcaaga tcatgctgct gagaaaaaag
     7381 ccagcggata ccgcaatcgg gagcgcttta agctgggaga gtgctcaact attggaaact
     7441 gaatatggcg ttcagagtct tcctgttatg gtcggagaag accctgattt gcacatgtaa
     7501 taatatctag aacttctttc ttgaaatcag agagggaaaa ttttagtttc tgagttgcaa
     7561 agtgtttttt tttttgttca ttcaaatagc tttcatgcgg tattattcct ttcttgtgag
     7621 tagcgtaaaa agtctattgt atctatttct tagtctccaa tatcttgaaa tgtctatctc
     7681 tcctagatat cctgtcacaa ttagactctt atatccataa tcaaccatga tctcttttaa
     7741 ttgttgtgcg aaggcggggg tcttagaatg tattcctgaa ttacaggata atatcctgat
     7801 atcgacaaag ttttttggca gacgtcgttc aagtaataaa atagctagtt gtctaggagt
     7861 taatgttgga cctgtttttg aacctgtacc aataactgat gtattgccat gagctacaac
     7921 atataatgtt cttggttttt taaaaagtgt aacggagttt taatcagatg ctctgatacc
     7981 ataacattta ttaccttttt tattttgaaa ttcaacttca ctttcaagaa atagatcatt
     8041 ctcatccatt agaaacggga agaaaaaaat aatcataacc accctattta gaataaaacc
     8101 tatgttttat tgggaaatag aaataaattc tatgaaatta attgttaaaa aataattagt
     8161 tttccagttc aagtcttgtc atccgaatcc gatcgaacag tgtgtgtttc taaatacgat
     8221 tgggttgatc gcagaaatat gatcaagtgc ttcttgagtc tggcgttctt cctcaagcgc
     8281 gacttcaggt gcttaacctt ggacacctcc atactgccat attcttgtgc taggtgtaaa
     8341 agtggcatcg gaaatagcct gcttacggca gagctcacgg gcagaaatca caacttcggc
     8401 cttgcgagga tgatgatctg ttcgtcggaa aaacacgtct aatcaacgga gagcagtcag
     8461 ttcatatcaa ccgctctgtg tatacatcaa taattactga caacgatata tgtaaatata
     8521 ccgacatata tttttgtttt tttcctgtag atataatttt agagtattta catcttctga
     8581 tttagacaat ccttcgtttg gttcaaagat cctcaattcc acatttgtct ttatgtatgg
     8641 caattgcatg acagtcatgg acatatacaa cattaattat aaattcacca tcaggtaatg
     8701 atgccggatg aaattcatat ttgggcatgc ctcccaaaat aaatatgcat gctttctatc
     8761 cccaatccta atcttcattt ggagatagaa ctgcatcctg tatctttccg gcttcagctc
     8821 cataacttaa agctctttga taggatgcga acctataaag ttgactgaaa taaataaaat
     8881 tacatggtat ttttttcgcc gtctgtctat aaaatcaatg cacatagata aacacccccc
     8941 cttatgtaaa gagacatcta aaattcttga cccctaaaac ttcatttggt ttaaatgaaa
     9001 ctcgaaatca acattcataa ttttccaatt tttacaaaga aagttttcat atcaatacaa
     9061 caacatttct taaaacaaac tatatatacg tagcggtgtt ttaacaccaa aaaatagact
     9121 gaccttatat ttattaataa ccagtgtttc atttatttta tctgtggtcc tgtaaaatca
     9181 tatatactat aatttgaata cccaagcctc ccccaattga aatttaatgg atcttgtatt
     9241 gttattttta ctatgtttga tatttactga agtaattaaa aaacataatg attgaccccc
     9301 ctaaaaatcc aaagtcagcc atacctattt taagaggcta ataggcccat attttaagag
     9361 ggcgtatttg taatactcaa tatttctagg gagaaaaatt ctatgacaaa aactatcctc
     9421 aggggaaaaa ccataactta atcggctccg ttttgcctta tcctttaata aaggatagga
     9481 cagattgttt ttattacaat accagttcag aaagcatcct gaaaatacca accacagttc
     9541 agatcaacaa atccacctat ccccggacgc cactggtgtt tcatatctac tctatacata
     9601 aagtccataa attaccgcca cacattaaaa taactatatc tatattaaat tacagtatga
     9661 gactacatct ttatactgcg atttctaatt tttaattcac taatctcaca gttgaactta
     9721 ttaatatgat gctgtattat caatttcttt taatatcatt tttatccata ttcatattat
     9781 tatttaattt tgatattctt atattcaccg gagtttcatg ctcaaggaaa gtatttttca
     9841 tatc
//
