LOCUS       CP077070                4229 bp    DNA     circular BCT 27-JUN-2021
DEFINITION  Escherichia coli strain Q4552 plasmid pECQ4552_IHU07, complete
            sequence.
ACCESSION   CP077070
VERSION     CP077070.1
DBLINK      BioProject: PRJNA738695
            BioSample: SAMN19756648
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Hamame,A., Davoust,B., Hamel,M., Rolain,J.-M. and Diene,S.M.
  TITLE     Genomic characterization of a mcr-1 and mcr-3-producing Escherichia
            coli strain isolated from pig in France
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Hamame,A., Davoust,B., Hamel,M., Rolain,J.-M. and Diene,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Aix-Marseille University, MEPHI, IRD, APHM,
            IHU-Mediterranee Infection, Marseille, France, 19-21 Bd Jean Moulin,
            Marseille 13005, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 34.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 12:56:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,716
            Genes (coding)                    :: 3,694
            CDSs (with protein)               :: 3,694
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 1,022
            CDSs (without protein)            :: 1,022
            Pseudo Genes (ambiguous residues) :: 0 of 1,022
            Pseudo Genes (frameshifted)       :: 875 of 1,022
            Pseudo Genes (incomplete)         :: 170 of 1,022
            Pseudo Genes (internal stop)      :: 38 of 1,022
            Pseudo Genes (multiple problems)  :: 59 of 1,022
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from MEPHI, IHU-Mediterranee
            infection, 19-21 Bd Jean moulin 13005, Marseille.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Q4552"
                     /isolation_source="pig"
                     /db_xref="taxon:562"
                     /plasmid="pECQ4552_IHU07"
                     /geo_loc_name="France: Avignon"
                     /lat_lon="43.85274931 N 4.96206883 E"
                     /collection_date="2020-01"
                     /collected_by="Afaf Hamame"
     gene            827..1822
                     /locus_tag="KRX18_24125"
     CDS             827..1822
                     /locus_tag="KRX18_24125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QWZ14472.1"
                     /translation="MQNPKLKNLTDYSPDDKPWDVHKSQSDDVGGIYLRAAEFEAYAAR
                     MRDCGGLLRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDARWMFLTLTVRNCAIEDLGDTLTAMNAAFQRMKVRKEFKSVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPGMLNGHDYVKHYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPDWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDPD"
                     /name="protein rep"
     gene            2961..3956
                     /locus_tag="KRX18_24130"
     CDS             2961..3956
                     /locus_tag="KRX18_24130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="QWZ14471.1"
                     /translation="MQNPKLKNLTDYSPDDKPWDVHKSQSDDVGGIYLRAAEFEAYAAR
                     MRDCGGLLRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDARWMFLTLTVRNCAIEDLGDTLTAMNAAFQRMKVRKEFKSVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPGMLNGHDYVKHYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPDWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDPD"
                     /name="protein rep"
     Typing          complement(1543..1733)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.375"
                     /coverage="98.0"
                     /evalue="1.4200000000000001e-44"
     Typing          complement(3677..3867)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.375"
                     /coverage="98.0"
                     /evalue="1.4200000000000001e-44"
ORIGIN
        1 ttgtacgact gcacccctca tacccttgtg atgggtgatg gttcacatgg tgagccatcg
       61 ccctgtaaag ccgcttttta cccccttcaa ggcgttgcct gtggggtagg tgcgtaagcc
      121 ctgccctaca ggtgatgccg tgggggtatt ttcgttttgc tcggtatgcc ttattcgttt
      181 aaatccgcca tcaggagtat gcggggtaaa gtcgtgcggc cgcgatgagg aacggggatt
      241 gcccccgcag ctctgcgagg aggcacacgc cgttcggaat cggggtccac tctgtttaat
      301 agtggactct tgttgcgatt tgcaacggaa acagccgcgt gcagtgaatg taatactgca
      361 cgtttgtctc aatctgacat ttctcagggg gcgatttaac gtggtttgct taatttttga
      421 gcttacgaat gcttgagtgt ttgagtgcct gaatgctgct tttgggggtt ggtttttcgg
      481 ccctgaaagg ggcgaaatcc ttgtatttat acttaaggga taactctagg attcaaattc
      541 gcttccagcc cttgtgccgc aaggcctgag agccaatttg ctcgaaaatg gttttgtgag
      601 tatgatttct gacaggtgat cgtgtccgcg aactaatttt tgattacttg gggtggttcc
      661 caagacctta cttactgaca cgatgatatg agaaacccct gcaagtgcta ccaacacttc
      721 aggggcgcga tcacctggct aaatacggtt tgaaatcgac tcgcaacagg tgatacggaa
      781 atgataacac acccttccag cgcctgccca tattggagca ggtgctatgc agaatcctaa
      841 gctaaagaac cttactgact acagtcccga cgataagccg tgggacgttc acaagtcgca
      901 atctgatgat gttggcggta tctatctgcg tgcagctgaa tttgaggcgt atgcggctcg
      961 tatgcgcgat tgtggcggct tgctgcgttt tggctggtct acccttaaag acaccggaga
     1021 aacgcgttta cggcttcgtg aggcgcattt ctgccgcgtt cgtcactgcc ctgtctgcca
     1081 gtggaggcgt tccctgatgt ggcaagcccg tttttatcag tctctgccga aaatcgtcgc
     1141 tgattaccct gatgcccgct ggatgttcct cacgctcacc gttcgcaact gtgcgatcga
     1201 ggatctcggg gacacgctta ctgccatgaa tgccgcattc cagcgcatga aggttcgcaa
     1261 ggaatttaaa tccgtacagg gatggattcg caccacagaa gttacccgcg gtcgtgatgg
     1321 ctctgcacac ccccactttc acaccctgat gatggttcct cctgggatgc tgaacggtca
     1381 tgactacgtt aagcactacc gctgggttga actctggcgt gactgcctgc gcgttgatta
     1441 cgatcctaat gttgatgttc gggcagtgaa accccgcaag cctaaagatg gccagactct
     1501 ggcatgtgcc actgctgaac tggtgcgcgg tgccgttgct gaaaccctga aatattcgac
     1561 aaagccggct gatatggttg ccgacccgga ctggttctta gagctgaccc ggcagacgca
     1621 taaacgccgt tttgttgcga ccggtggcgc tctgaaagac gttctgaaac tggagcagga
     1681 aacggatcag gacatggtga tcggggatga tatttccgat ggtgaagacg atggttcacg
     1741 cctcgccttt ggctgggatt cgacgccaag aaaatacgtc cgtttgcctg aacgcgataa
     1801 gtttaaatca gatcctgatt agggtgtcgg ggcctgccct gaccaggttg tttttggatg
     1861 ccgctgcgtg tggcggtact ccaaaaacac atcttgtccg gtacacagat ttcctttggc
     1921 attccttcgg gtttccctga gatttccact ctgcatacat acacgcatac acgaataatc
     1981 aacggcagaa ttcgggcagg atatgtaaag tgggaccact ttacgcctgc cttattcgca
     2041 ctctgctcaa tggaatatta aatctgacag cgttcgccgc cgtcgaatcg tcaacttcta
     2101 aatcggggat ccctttaggg ttccgattta gtggttgtac gactgcaccc ctcataccct
     2161 tgtgatgggt gatggttcac atggtgagcc atcgccctgt aaagccgctt tttaccccct
     2221 tcaaggcgtt gcctgtgggg taggtgcgta agccctgccc tacaggtgat gccgtggggg
     2281 tattttcgtt ttgctcggta tgccttattc gtttaaatcc gccatcagga gtatgcgggg
     2341 taaagtcgtg cggccgcgat gaggaacggg gattgccccc gcagctctgc gaggaggcac
     2401 acgccgttcg gaatcggggt ccactctgtt taatagtgga ctcttgttgc gatttgcaac
     2461 ggaaacagcc gcgtgcagtg aatgtaatac tgcacgtttg tctcaatctg acatttctca
     2521 ggggggcgat ttaacgtggt ttgcttaatt tttgagctta cgaatgcttg agtgtttgag
     2581 tgcctgaatg ctgcttttgg gggttggttt ttcggccctg aaaggggcga aatccttgta
     2641 tttatactta agggataact ctaggattca aattcgcttc cagcccttgt gccgcaaggc
     2701 ctgagagcca atttgctcga aaatggtttt gtgagtatga tttctgacag gtgatcgtgt
     2761 ccgcgaacta atttttgatt acttggggtg gttcccaaga ccttacttac tgacacgatg
     2821 atatgagaaa ccctgcaagt gctaccaaca cttcaggggc gcgatcacct ggctaaatac
     2881 ggtttgaaat cgactcgcaa caggtgatac ggaaatgata acacaccctt ccagcgcctg
     2941 cccatattgg agcaggtgct atgcagaatc ctaagctaaa gaaccttact gactacagtc
     3001 ccgacgataa gccgtgggac gttcacaagt cgcaatctga tgatgttggc ggtatctatc
     3061 tgcgtgcagc tgaatttgag gcgtatgcgg ctcgtatgcg cgattgtggc ggcttgctgc
     3121 gttttggctg gtctaccctt aaagacaccg gagaaacgcg tttacggctt cgtgaggcgc
     3181 atttctgccg cgttcgtcac tgccctgtct gccagtggag gcgttccctg atgtggcaag
     3241 cccgttttta tcagtctctg ccgaaaatcg tcgctgatta ccctgatgcc cgctggatgt
     3301 tcctcacgct caccgttcgc aactgtgcga tcgaggatct cggggacacg cttactgcca
     3361 tgaatgccgc attccagcgc atgaaggttc gcaaggaatt taaatccgta cagggatgga
     3421 ttcgcaccac agaagttacc cgcggtcgtg atggctctgc acacccccac tttcacaccc
     3481 tgatgatggt tcctcctggg atgctgaacg gtcatgacta cgttaagcac taccgctggg
     3541 ttgaactctg gcgtgactgc ctgcgcgttg attacgatcc taatgttgat gttcgggcag
     3601 tgaaaccccg caagcctaaa gatggccaga ctctggcatg tgccactgct gaactggtgc
     3661 gcggtgccgt tgctgaaacc ctgaaatatt cgacaaagcc ggctgatatg gttgccgacc
     3721 cggactggtt cttagagctg acccggcaga cgcataaacg ccgttttgtt gcgaccggtg
     3781 gcgctctgaa agacgttctg aaactggagc aggaaacgga tcaggacatg gtgatcgggg
     3841 atgatatttc cgatggtgaa gacgatggtt cacgcctcgc ctttggctgg gattcgacgc
     3901 caagaaaata cgtccgtttg cctgaacgcg ataagtttaa atcagatcct gattagggtg
     3961 tcggggcctg ccctgaccag gttgtttttg gatgccgctg cgtgtggcgg tactccaaaa
     4021 acacatcttg tccggtacac agatttcctt tggcattcct tcgggtttcc ctgagatttc
     4081 cactctgcat acatacacgc atacacgaat aatcaacggc agaattcggg caggatatgt
     4141 aaagtgggac cactttacgc ctgccttatt cgcactctgc tcaatggaat attaaatctg
     4201 acagcgttcg ccgccgtcga atcgtcaac
//
