LOCUS       CP077069                7870 bp    DNA     circular BCT 27-JUN-2021
DEFINITION  Escherichia coli strain Q4552 plasmid pECQ4552_IHU06, complete
            sequence.
ACCESSION   CP077069
VERSION     CP077069.1
DBLINK      BioProject: PRJNA738695
            BioSample: SAMN19756648
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Hamame,A., Davoust,B., Hamel,M., Rolain,J.-M. and Diene,S.M.
  TITLE     Genomic characterization of a mcr-1 and mcr-3-producing Escherichia
            coli strain isolated from pig in France
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Hamame,A., Davoust,B., Hamel,M., Rolain,J.-M. and Diene,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Aix-Marseille University, MEPHI, IRD, APHM,
            IHU-Mediterranee Infection, Marseille, France, 19-21 Bd Jean Moulin,
            Marseille 13005, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 34.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 12:56:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,716
            Genes (coding)                    :: 3,694
            CDSs (with protein)               :: 3,694
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 1,022
            CDSs (without protein)            :: 1,022
            Pseudo Genes (ambiguous residues) :: 0 of 1,022
            Pseudo Genes (frameshifted)       :: 875 of 1,022
            Pseudo Genes (incomplete)         :: 170 of 1,022
            Pseudo Genes (internal stop)      :: 38 of 1,022
            Pseudo Genes (multiple problems)  :: 59 of 1,022
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from MEPHI, IHU-Mediterranee
            infection, 19-21 Bd Jean moulin 13005, Marseille.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Q4552"
                     /isolation_source="pig"
                     /db_xref="taxon:562"
                     /plasmid="pECQ4552_IHU06"
                     /geo_loc_name="France: Avignon"
                     /lat_lon="43.85274931 N 4.96206883 E"
                     /collection_date="2020-01"
                     /collected_by="Afaf Hamame"
     gene            397..712
                     /locus_tag="KRX18_24065"
                     /pseudo=""
     CDS             397..712
                     /locus_tag="KRX18_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(906..1424)
                     /locus_tag="KRX18_24070"
     CDS             complement(906..1424)
                     /locus_tag="KRX18_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWZ14467.1"
                     /translation="MKCFWACSVSVVLVMSFTVASAENITYTKPSAGRDLYLTCKGYQV
                     KGASVINVEKPSYLEGTGEQVEFAIISLGDASDGLKVFFDSWGFSSFGLIESHVMHEHW
                     SLVHVGGRVSVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCINSDKRPSWVEKIYTRT
                     GVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(1553..1768)
                     /locus_tag="KRX18_24075"
     CDS             complement(1553..1768)
                     /locus_tag="KRX18_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QWZ14468.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQV"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1772..2257)
                     /locus_tag="KRX18_24080"
     CDS             complement(1772..2257)
                     /locus_tag="KRX18_24080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QWZ14469.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAILDHDRKLSSAMRQRTKGMVRMVSQTWLTIVLVSALLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(2270..2944)
                     /locus_tag="KRX18_24085"
     CDS             complement(2270..2944)
                     /locus_tag="KRX18_24085"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="QWZ14470.1"
                     /translation="MIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELID
                     ASPYAKKYTSGVLSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLE
                     LNFLIPNTELLTGKRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSAL
                     PEAKQEAAQAITRGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGET
                     SD"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2934..3256)
                     /locus_tag="KRX18_24090"
                     /pseudo=""
     CDS             complement(2934..3256)
                     /locus_tag="KRX18_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4438..4753
                     /locus_tag="KRX18_24095"
                     /pseudo=""
     CDS             4438..4753
                     /locus_tag="KRX18_24095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4947..5464)
                     /locus_tag="KRX18_24100"
                     /pseudo=""
     CDS             complement(4947..5464)
                     /locus_tag="KRX18_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246513.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5593..5808)
                     /locus_tag="KRX18_24105"
     CDS             complement(5593..5808)
                     /locus_tag="KRX18_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QWZ14464.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQV"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5812..6297)
                     /locus_tag="KRX18_24110"
     CDS             complement(5812..6297)
                     /locus_tag="KRX18_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QWZ14465.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAILDHDRKLSSAMRQRTKGMVRMVSQTWLTIVLVSALLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(6310..6948)
                     /locus_tag="KRX18_24115"
     CDS             complement(6310..6948)
                     /locus_tag="KRX18_24115"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="QWZ14466.1"
                     /translation="MAPVRWIICWGKTDSATAPPFCRGSRKKSGSLSTPRPTQKYTSGV
                     LSFAEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNTELLT
                     GKRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPEAKQEAAQAIT
                     RGLLALASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGETSD"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6972..7294)
                     /locus_tag="KRX18_24120"
                     /pseudo=""
     CDS             complement(6972..7294)
                     /locus_tag="KRX18_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3358..3543)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.895"
                     /coverage="87.0"
                     /evalue="4.47e-55"
     Typing          complement(7396..7581)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.895"
                     /coverage="87.0"
                     /evalue="4.47e-55"
     Typing          3519..4004
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.298"
                     /coverage="94.0"
                     /evalue="1.3e-153"
     Typing          7557..7870
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.663"
                     /coverage="94.0"
                     /evalue="1.13e-69"
     Typing          complement(1462..2297)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.738"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2535..2944)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.401"
                     /coverage="83.0"
                     /evalue="1.03e-69"
     Typing          complement(5502..6337)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.738"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6545..6948)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.222"
                     /coverage="80.0"
                     /evalue="1.11e-67"
     Typing          complement(6976..7193)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.667"
                     /coverage="100.0"
                     /evalue="1.27e-32"
ORIGIN
        1 gcgataaggc tacactgaaa ggacagtttt ggtgactgtg ctcctccaag ccagttacct
       61 tggttcaaag agttgctgct ctgagaacct tcgaaaaccg ccttgcaggg cggttttttc
      121 gtttcgtgga cacgatacgc gcgatcgaag agaaaggtca agtcatctta ctcaacccaa
      181 gaatagccta actggtcgtt aacctaccaa aattatgatc ccatacatgc acagcaacaa
      241 catctgaaag ccgtcccatt aggcggcttt ttagtgtctg ctattcaaac acatttacta
      301 taaaacgcac tctaggcatc catcactgcc gtatctttct ttatcagtca tttggttatt
      361 gggtaagcta taatttttgc aaaaaaagga ttaaagatgg ctgttacatg cccaaaatgt
      421 ggttcagata aaacacagaa aagaaacatc ggtacaaagg tagcaggtgg ggctggtgca
      481 accgctggtg gaattggcgc aatcgccggt gggagtgaag gagccaccgt aggagctgca
      541 attggttcag caatattccc tggtatcggg actgttgccg ggggttatcg gttttcttgg
      601 cggtctggca gcaggggcgg ctgtgggtgc tgctgcagga aaagcagtag atgaatacat
      661 cttagataag tacaaatgta atgcctgtaa aaataccttc agtgatgact gatttaccat
      721 aacgtcgcat tgcgcgcacc gctcagacgc gctcagggcg tttttctgtc ctgagccaca
      781 aaagagcccg tcttctcacc gaaaaccgcc cggaatcgcg ctgataagtt ttgcgtgtat
      841 gcatagccat gcacgaaaat gaactaacac tctgttcaat gaattttcat gctgtacgca
      901 gtattttagt cttctatgcc ccctcgaaac acgcctgttc ttgtgtagat tttttcgacc
      961 caagatggcc ttttatcaga gttaatgcag ccatacagga acacacctgt tttgggttta
     1021 tagctcccac cctcctgtgg ctggttatct acaaatgaat aatcaatctt ttcacccttg
     1081 actgaaacac gccccccaac atgaactaaa gaccagtgct catgcattac atgactttca
     1141 atcaatccaa aagaagaaaa accccatgaa tcaaaaaaaa ccttgagtcc atcagatgca
     1201 tcaccgagag aaatgattgc aaactcaacc tgctcacctg taccttcaag gtaagaaggt
     1261 ttctcgacgt taattacaga cgcccccttc acctgataac ctttacaggt aagatataag
     1321 tccctcccgg ctgacggttt tgtgtatgta atgttctccg cagaagcgac tgtaaatgac
     1381 ataaccaaaa ctactgagac tgagcaagcc cagaaacact tcataacgca tcccttttca
     1441 ttgattaact acattccaag ctcaaaacct ttctgccgct gcgatcgttc cagctccagc
     1501 gttttctgat gctgcgccgc ccactcctgc tgcctgtatg cttcaatcga gttcagactt
     1561 gtgacaaccg gcgcagctgc tcgctcaaac tctgcacctg cctgctcaag cccgtgacgc
     1621 gctcgctcag cgccgcgttc tcccgttgca taagaccaga catcgtccgc cattccgcga
     1681 aggcgctctc ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc
     1741 tcagcaactg tttttccagc tccgtcatgt gctatttccc cgccagtatc atccagctcg
     1801 aatcctctcc gaaccgtccc gcttccgggt tcaccctcac gcagcggcgt ctctgctctc
     1861 cgcaggtcga gagctgcacg ccgctgttcc tctcggacag catggcctgc gtgcgcttct
     1921 gctcccggat ggtcgtgtaa ttgtcgagta tctgctgccc ctgccaccac agaatgcttg
     1981 cgcccgatgc tgtcagcagt gcagacacca ggacgatggt cagccacgtc tggctgacca
     2041 tacgcaccat gcctttcgtg cgctgtctca tggctgagga cagcttccgg tcgtggtcga
     2101 ggatggcggc gctgattctc ttctcgcttt cgttcagttc cgctctgaca gacttctcgt
     2161 gctcgctgaa tgcggctttc agcatctcgc cggtattctg ctgctgcgct ttcgatttct
     2221 gctctaagtc cttcgccagc gttaaaagac tgttcataaa tggctccctt cagtcggatg
     2281 tttcgccccc gtccgggtcg gcaatgctga tactgctttt tgtggtgcgg accacctcaa
     2341 aacctgcgct ttccagcgcc tcagtgacgt cctgacgggt tttcagctcc ccggatgagg
     2401 caagggcaag caaaccgcgt gtaatcgctt gtgcggcctc ctgtttcgct tccggcagcg
     2461 cagatggcgt caccagcgcc cgtcggtttt ccggcgcgtt cgggtcatgc agccccagcc
     2521 tgccgttcac gatggtctgc caggcatcaa tgcgcggacg gtcggcgcgg tcgtaatacg
     2581 gctggagacg cttgccggtc agcagctccg tgttcgggat caggaagttc agctccagcc
     2641 gccccttatc ctggtgctca acccacagca cgctgtactg gtctttatcg agtccgggca
     2701 tcagaacccg ttcaaagctc gccatcagct tttcgcgctg cccgggcggt aaatcctttt
     2761 cggcaaagga caggacgccg gatgtgtact tttttgcgta gggcgaggcg tcgataagct
     2821 ccctgacttc ttccggcttc ccctgcagaa cggtggcgcc gtcgcgctgt cggtctttcc
     2881 ccagcagata atccaccgga ccggcgccac cgccgcgccc tcgcgggtga aacttaacga
     2941 tcatcgtctg cacccttttc cagcacggca tgccgcagcc gctcgagtcc ggcatctatg
     3001 gccatcagcg cggcgacgac ctgcacacgg tcgtgcccgc tgccaccacc ggcgttaacc
     3061 tgccgggcaa tctggttgag gttgttcccc atgccggcaa gctgacgaag cagcgccggc
     3121 gagatcgagg aagcttgccg gcgcgggcgg gtttctcgtc caggcacgtc tggcgcatcc
     3181 aggctgcaag ctgtttaccg tcgcagcgct ccagcagacg gcggtgctca tcttcggtca
     3241 cccacatcgt gagcattttg ctgcgtttgt ctgccaatcc ggcctcctga taacgccggg
     3301 acgggcggga aatcttttcc cggtcggcgc ggtgttggac aagccgcagg cgcgtcagtg
     3361 gttttagcgg gtttcgggga gcagccctga accagtcacg tagcgctagc ggagtgtata
     3421 ctggctgaat atgttagttc ggagtggtaa ttcagggaag ttattcatgt tgcaagggaa
     3481 aatgcggcta tagggcgtaa gtgcaaaatg caggtaacgg tgacatgacg cctcctcgct
     3541 cactcggtcg ctacgctcct gccgtgaggc tgcggcgggc gttaccttct cacgaatgac
     3601 aagataaaga gatggtactc aagtcaggaa aggccggaaa aaggatacgg cgctgccgtt
     3661 tttccatagg ctccgccccc tgacgagcat cacaaaatca gacgctcaaa tcagtggtgg
     3721 cgaaacccga tgcggcttag agataccagg cgtttccccc tgatggctcc ctcgtgctcc
     3781 cccgcattcc tgcccttcgg tttaccggtg tcattccgct gttatggccg cgtttgtctc
     3841 attccacgcc tgacactcag ttccgggtag gcagttcgct ccaggatgga ctgtatgcac
     3901 gaacccccgt tcagtccgac tgctgcgcct tgtccggtaa ctatcgtctt gagtccaacc
     3961 cggaaagaca tgcaaaagcg ccactggcag cagccactgg taacaggttt agaggaggag
     4021 agtcttgaaa tttcgggtgc gataaggcta cactgaaagg acagttttgg tgactgtgct
     4081 cctccaagcc agttaccttg gttcaaagag ttgctgctct gagaaccttc gaaaaaccgc
     4141 cttgcagggc ggttttttcg tttcgtggac acgatacgcg cgatcgaaga gaaaggtcaa
     4201 gtcatcttac tcaacccaag aatagcctaa ctggtcgtta acctaccaaa attatgatcc
     4261 catacatgca cagcaacaac atctgaaagc cgtcccatta ggcggctttt tagtgtctgc
     4321 tattcaaaca catttactat aaaacgcact ctaggcatcc atcactgccg tatctttctt
     4381 tatcagtcat ttggttattg ggtaagctat aatttttgca agaaaaaagg attaaagatg
     4441 gctgttacat gcccaaaatg tggttcagat aaaacacaga aaagaaacat cggtacaaag
     4501 gtagcaggtg gggctggtgc aaccgctggt ggaattggcg caatcgccgg tgggagtgaa
     4561 ggagccaccg taggagctgc aattggttca gcaatattcc ctggtatcgg gactgttgcc
     4621 gggggttatc ggttttcttg gcggtctggc agcaggggcg gctgtgggtg ctgctgcagg
     4681 aaaagcagta gatgaataca tcttagataa gtacaaatgt aatgcctgta aaaatacctt
     4741 cagtgatgac tgatttacca taacgtcgca ttgcgcgcac cgctcagacg cgctcagggc
     4801 gtttttctgt cctgagccac aaaagagccc gtcttctcac cgaaaaccgc ccggaatcgc
     4861 gctgataagt tttgcgtgta tgcatagcca tgcacgaaaa tgaactaaca ctctgttcaa
     4921 tgaattttca tgctgtacgc agtattttag tcttctatgc cccctcgaaa cacgcctgtt
     4981 cttgtgtaga tttttcgacc caagatggcc ttttatcaga gttaatgcag ccatacagga
     5041 acacacctgt tttgggttta tagctcccac cctcctgtgg ctggttatct acaaatgaat
     5101 aatcaatctt ttcacccttg actgaaacac gccccccaac atgaactaaa gaccagtgct
     5161 catgcattac atgactttca atcaatccaa aagaagaaaa accccatgaa tcaaaaaaaa
     5221 ccttgagtcc atcagatgca tcaccgagag aaatgattgc aaactcaacc tgctcacctg
     5281 taccttcaag gtaagaaggt ttctcgacgt taattacaga cgcccccttc acctgataac
     5341 ctttacaggt aagatataag tccctcccgg ctgacggttt tgtgtatgta atgttctccg
     5401 cagaagcgac tgtaaatgac ataaccaaaa ctactgagac tgagcaagcc cagaaacact
     5461 tcataacgca tcccttttca ttgattaact acattccaag ctcaaaacct ttctgccgct
     5521 gcgatcgttc cagctccagc gttttctgat gctgcgccgc ccactcctgc tgcctgtatg
     5581 cttcaatcga gttcagactt gtgacaaccg gcgcagctgc tcgctcaaac tctgcacctg
     5641 cctgctcaag cccgtgacgc gctcgctcag cgccgcgttc tcccgttgca taagaccaga
     5701 catcgtccgc cattccgcga aggcgctctc ccactcgtcc agccttttcg agtagtcctg
     5761 ctgtagctgc tctaatgcgc tcagcaactg tttttccagc tccgtcatgt gctatttccc
     5821 cgccagtatc atccagctcg aatcctctcc gaaccgtccc gcttccgggt tcaccctcac
     5881 gcagcggcgt ctctgctctc cgcaggtcga gagctgcacg ccgctgttcc tctcggacag
     5941 catggcctgc gtgcgcttct gctcccggat ggtcgtgtaa ttgtcgagta tctgctgccc
     6001 ctgccaccac agaatgcttg cgcccgatgc tgtcagcagt gcagacacca ggacgatggt
     6061 cagccacgtc tggctgacca tacgcaccat gcctttcgtg cgctgtctca tggctgagga
     6121 cagcttccgg tcgtggtcga ggatggcggc gctgattctc ttctcgcttt cgttcagttc
     6181 cgctctgaca gacttctcgt gctcgctgaa tgcggctttc agcatctcgc cggtattctg
     6241 ctgctgcgct ttcgatttct gctctaagtc cttcgccagc gttaaaagac tgttcataaa
     6301 tggctccctt cagtcggatg tttcgccccc gtccgggtcg gcaatgctga tactgctttt
     6361 tgtggtgcgg accacctcaa aacctgcgct ttccagcgcc tcagtgacgt cctgacgggt
     6421 tttcagctcc ccggatgagg caagggcaag caaaccgcgt gtaatcgctt gtgcggcctc
     6481 ctgtttcgct tccggcagcg cagatggcgt caccagcgcc cgtcggtttt ccggcgcgtt
     6541 cgggtcatgc agccccagcc tgccgttcac gatggtctgc caggcatcaa tgcgcggacg
     6601 gtcggcgcgg tcgtaatacg gctggagacg cttgccggtc agcagctccg tgttcgggat
     6661 caggaagttc agctccagcc gccccttatc ctggtgctca acccacagca cgctgtactg
     6721 gtctttatcg agtccgggca tcagaacccg ttcaaagctc gccatcagct tttcgcgctg
     6781 cccgggcggt aaatcctttt cggcaaagga caggacgccg gatgtgtact tttgcgtagg
     6841 gcgaggcgtc gataagctcc ctgacttctt ccggcttccc ctgcagaacg gtggcgccgt
     6901 cgcgctgtcg gtctttcccc agcagataat ccaccggacc ggcgccaccg ccgcgccctc
     6961 gcgggtgaaa cttaacgatc atcgtctgca cccttttcca gcacggcatg ccgcagccgc
     7021 tcgagtccgg catctatggc catcagcgcg gcgacgacct gcacacggtc gtgcccgctg
     7081 ccaccaccgg cgttaacctg ccgggcaatc tggttgaggt tgttccccat gccggcaagc
     7141 tgacgaagca gcgccggcga gatcgaggaa gcttgccggc gcgggcgggt ttctcgtcca
     7201 ggcacgtctg gcgcatccag gctgcaagct gtttaccgtc gcagcgctcc agcagacggc
     7261 ggtgctcatc ttcggtcacc cacatcgtga gcattttgct gcgtttgtct gccaatccgg
     7321 cctcctgata acgccgggac gggcgggaaa tcttttcccg gtcggcgcgg tgttggacaa
     7381 gccgcaggcg cgtcagtggt tttagcgggt ttcggggagc agccctgaac cagtcacgta
     7441 gcgctagcgg agtgtatact ggctgaatat gttagttcgg agtggtaatt cagggaagtt
     7501 attcatgttg caagggaaaa tgcggctata gggcgtaagt gcaaaatgca ggtaacggtg
     7561 acatgacgcc tcctcgctca ctcggtcgct acgctcctgc cgtgaggctg cggcgggcgt
     7621 taccttctca cgaatgacaa gataaagaga tggtactcaa gtcaggaaag gccggaaaaa
     7681 ggatacggcg ctgccgtttt tccataggct ccgccccctg acgagcatca caaaatcaga
     7741 cgctcaaatc agtggtggcg aaacccgatg cggcttagag ataccaggcg tttccccctg
     7801 atggctccct cgtgctcccc cgcattcctg cccttcggtt taccggtgtc attccgctgt
     7861 tatggccgcg
//
