LOCUS       CP077068                8913 bp    DNA     circular BCT 27-JUN-2021
DEFINITION  Escherichia coli strain Q4552 plasmid pECQ4552_IHU05, complete
            sequence.
ACCESSION   CP077068
VERSION     CP077068.1
DBLINK      BioProject: PRJNA738695
            BioSample: SAMN19756648
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Hamame,A., Davoust,B., Hamel,M., Rolain,J.-M. and Diene,S.M.
  TITLE     Genomic characterization of a mcr-1 and mcr-3-producing Escherichia
            coli strain isolated from pig in France
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8913)
  AUTHORS   Hamame,A., Davoust,B., Hamel,M., Rolain,J.-M. and Diene,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Aix-Marseille University, MEPHI, IRD, APHM,
            IHU-Mediterranee Infection, Marseille, France, 19-21 Bd Jean Moulin,
            Marseille 13005, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 34.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 12:56:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,716
            Genes (coding)                    :: 3,694
            CDSs (with protein)               :: 3,694
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 1,022
            CDSs (without protein)            :: 1,022
            Pseudo Genes (ambiguous residues) :: 0 of 1,022
            Pseudo Genes (frameshifted)       :: 875 of 1,022
            Pseudo Genes (incomplete)         :: 170 of 1,022
            Pseudo Genes (internal stop)      :: 38 of 1,022
            Pseudo Genes (multiple problems)  :: 59 of 1,022
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from MEPHI, IHU-Mediterranee
            infection, 19-21 Bd Jean moulin 13005, Marseille.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Q4552"
                     /isolation_source="pig"
                     /db_xref="taxon:562"
                     /plasmid="pECQ4552_IHU05"
                     /geo_loc_name="France: Avignon"
                     /lat_lon="43.85274931 N 4.96206883 E"
                     /collection_date="2020-01"
                     /collected_by="Afaf Hamame"
     gene            complement(767..1270)
                     /locus_tag="KRX18_24025"
     CDS             complement(767..1270)
                     /locus_tag="KRX18_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QWZ14461.1"
                     /translation="MGTQEVITETQIKQRLLDLEEQNRKLQQELLEERKNTNFTQTYPK
                     GWERIRNLIQSNPGAARLYSVLSEHIDGNCGAVVADQQFLADQLSVTTRTIRNWVSFLE
                     ENNCLVKIPIAGKICAYALDPAEVWKGYNTSKPYAAFVTKTLVNSEGVIKRRLQACFGS
                     SEPE"
                     /name="helix-turn-helix domain-containing protein"
     gene            1801..2816
                     /locus_tag="KRX18_24030"
                     /pseudo=""
     CDS             1801..2816
                     /locus_tag="KRX18_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            complement(2878..3312)
                     /locus_tag="KRX18_24035"
     CDS             complement(2878..3312)
                     /locus_tag="KRX18_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QWZ14462.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="MarR family transcriptional regulator"
     gene            complement(4169..4672)
                     /locus_tag="KRX18_24040"
     CDS             complement(4169..4672)
                     /locus_tag="KRX18_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QWZ14463.1"
                     /translation="MGTQEVITETQIKQRLLDLEEQNRKLQQELLEERKNTNFTQTYPK
                     GWERIRNLIQSNPGAARLYSVLSEHIDGNCGAVVADQQFLADQLSVTTRTIRNWVSFLE
                     ENNCLVKIPIAGKICAYALDPAEVWKGYNTSKPYAAFVTKTLVNSEGVIKRRLQACFGS
                     SEPE"
                     /name="helix-turn-helix domain-containing protein"
     gene            5203..6219
                     /locus_tag="KRX18_24045"
     CDS             5203..6219
                     /locus_tag="KRX18_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="QWZ14458.1"
                     /translation="MRSPDWPDGRRNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="plasmid recombination protein"
     gene            complement(6281..6715)
                     /locus_tag="KRX18_24050"
     CDS             complement(6281..6715)
                     /locus_tag="KRX18_24050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="QWZ14459.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="MarR family transcriptional regulator"
     gene            complement(7572..8075)
                     /locus_tag="KRX18_24055"
     CDS             complement(7572..8075)
                     /locus_tag="KRX18_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="QWZ14460.1"
                     /translation="MGTQEVITETQIKQRLLDLEEQNRKLQQELLEERKNTNFTQTYPK
                     GWERIRNLIQSNPGAARLYSVLSEHIDGNCGAVVADQQFLADQLSVTTRTIRNWVSFLE
                     ENNCLVKIPIAGKICAYALDPAEVWKGYNTSKPYAAFVTKTLVNSEGVIKRRLQACFGS
                     SEPE"
                     /name="helix-turn-helix domain-containing protein"
     gene            8655..8913
                     /locus_tag="KRX18_24060"
                     /pseudo=""
     CDS             8655..8913
                     /locus_tag="KRX18_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     Typing          1802..2364
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.809"
                     /coverage="100.0"
                     /evalue="1.5899999999999998e-170"
     Typing          2365..2813
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.0"
                     /coverage="100.0"
                     /evalue="1.5899999999999998e-170"
     Typing          5204..6216
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.45"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caagggctga gagaaggctc tcttctgtat cttatagcga gcgtagcgag tgccatacct
       61 aaagtacgat tagtacttga gatgagtacg attagtactt gaggtgagta cgattagtac
      121 ttgaggtgag tacgattagt acttgaggtg agtacgattc ttctctctag cccttggtgt
      181 gcgcgggttc ccgtatctat atctatatat taaggggaaa taaaggctga aaaacgccat
      241 ttttaaacat cgaaaaacga gcttttttga gtctggagcg tacggatttc tatgtccggg
      301 gagtggaaat tccgcttcct cgatcggaca gactcgcggc gttgccgctc cctaatccga
      361 tctgcggcga gcggttcctg ctctttcaaa agcgatatcc agatcctgat ttcgtcaagg
      421 gcgagcggag cgagtgcatt tcacccttga cgacgcacaa ataaaacagg cttggcagtg
      481 gtgggaggtg acgcggagcg acgccttcca cctgccagac cgccgttgat tttgaccttc
      541 tggcagcttg ctgcccgaac gattttgcga agcaaagtct agtgagtaca ccttctgaaa
      601 gatggtggcc tgtcagagac ggttttgatg ttcgggaagg tcaactgcaa gggcggctta
      661 acaggtggat ggctcacttt gttcaccacc cacctttgcc aagtgttggg gtcgttgcag
      721 cccgtcaagg gtacgcatac gccgctaacg cgcccctgac tggctttcac tccggctctg
      781 agcttccgaa gcaggcctgt agacgccttt tgattacacc ttcgctgttt actagcgttt
      841 tggtaacgaa ggctgcatat ggctttgatg tgttataccc cttccaaact tcggcgggat
      901 ctagggcgta tgcgcagatc ttcccagcaa tagggatttt cactaaacag ttgttttctt
      961 ccaagaaact aacccaattc cttatcgttc tggttgttac ggaaagctgg tccgctaaga
     1021 attgttggtc tgctacaact gctccgcagt tgccatcgat gtgttctgat aggacggaat
     1081 acaacctagc agctcctgga ttgctctgta tgaggtttct aatcctctcc catccctttg
     1141 ggtaggtttg agtgaaattc gtgttcttac gctcttctag gagctcctgt tgcagttttc
     1201 tgttttgctc ttccaagtct aagagtcttt gtttgatttg agtctctgta attacttctt
     1261 gtgttcccat cagctccccc tcgaatttcc acttaaccgg aatttatgtg tattttaagt
     1321 gttgtttcat ttcctgaata tcaaatataa cttcccatgt tcggaaagta taggaaatac
     1381 agggtttccg gttaaccgga aatacagggt ttcctatact atttctgaaa acacatataa
     1441 atcagtaagt tatagaatat tcccttctta tctaaaagat cccactgctc gcagcgggat
     1501 cttgaagtag ctcctccctt cggtcgggca tttttggggt caaggttcgc catcggcgac
     1561 cgcgttagcg gtttaacctt gacgctaaaa atgacctacc aaacttgtcg agggtgatgg
     1621 ttcgcatagc gagccatcgc cctgcaagac ggttttgatt ttgaggtttc ggggcgtagc
     1681 cctgaaccag cacagagacc cctctgtgta tgctggctta atgcagttac gagcgatttt
     1741 gcgtagcaaa gtctagtgag tacactatca tcctgatagt ggcccgccgg aggcatcgca
     1801 atgcgatccc ccgactggcc ggatggacgc cggaacgagg gggtaagcat ggcgaattac
     1861 gcgataatgc gctgcaaaaa gctgacggga atgggcagtg tggccagcgc gttgcagcac
     1921 tgctacaggg agcgtgagac gccgaatgct gacgctgaac ggacaccaga aaactactgc
     1981 tctgtgtctc agtcggcaga tgaggcgatg ggaaagctgc gggagctgtt gccggaaaaa
     2041 cgccgtaaag atgcggtgct ggctgttgaa tacgttatga cggcaagccc tgagtggtgg
     2101 aacgaggcca caccgcggca gcaggcggag tttttcgccc gctctgagca atggctggag
     2161 aagaaatacg ggaaggatcg tgttgtggcg gcggtggttc accgggacga ggccaccccg
     2221 cacctgtctg cgttcgtggt gccattgacg caggatggac ggctgtccgc gaaagagttt
     2281 attggcgggc ggtcaaagat gcgtgaagat cagagcacct acgctgaatc agtaaaaaaa
     2341 ctgggtcttg agcgcggcat cgaggaagcc gcgccacgca tcagacagtt cagcactact
     2401 acgaatcgat aaaccgtggc acccgtagcc aggtatcgat ctcgccggag gcgctggagc
     2461 cgcgtgtatt gcggaagggc attttcacca aagacgtgga agatcaggca gccatagcca
     2521 aacgtctaag ccacgcagtg aacgatggct ttgcaggaac catcgctatg gcctctcaga
     2581 gcgcccagaa cgcaaaaaga gcgcgtgagc tacagaagac tatggatgcg cagcagaaac
     2641 gcctacagag cgttacagag ccttttaagg ggctttccag agagcagatg accgaaatcc
     2701 tgatgatggc tcagcggttt aagcagcaaa accaggaaaa ggaaaaacag cagcgtattg
     2761 aacgtgaaaa acagcgacag acgcgatcgc gtggcatggg tggtatggag cgttagtttt
     2821 gatgcgatgc aggatagggc taacgccgca aagtgacgtc atttgcggca ttccagatta
     2881 ggttctgccg ccctgtataa cgctgaggcc gttttttagt gctttcttgt ggttgctgac
     2941 agtacctttc cagccaaact gagggttcag acggtaactg taactgcgcc ctacttttgg
     3001 gccttgaaga atcacggata ggtcaattag cgccttaacc gaacggctta cattggtttt
     3061 ctgcatacct aaagcgtcgg caatatcggc ttgggcaatt tggatgtaat tttcataatc
     3121 gagatcagct aacagcatca tcaagacttt gaattgctcc cctgtgaggg tcttagcaag
     3181 gatctttaaa gcgtcttggt tcatagtgaa atgcctctca aaagctgact tctgtttggg
     3241 tcggataact gcgacaaaac tacctacctc ttcccctgta gcagtgtcta tctgagtaat
     3301 tcggttactc atttaagagg ctctcccaaa aaaatagtta tcaataatga taacaacgtt
     3361 atcaataatg ataactatcg tcaagcgaga gccgcgcccc acaagggctg agagaaggct
     3421 ctcttctgta tcttatagcg agcgtagcga gtgccatacc taaagtacga ttagtacttg
     3481 agatgagtac gattagtact tgaggtgagt acgattagta cttgaggtga gtacgattag
     3541 tacttgaggt gagtacgatt cttctctcta gcccttggtg tgcgcgggtt cccgtatcta
     3601 tatctatata ttaaggggga aataaaggct gaaaaacgcc atttttaaac atcgaaaaac
     3661 gagctttttt gagtctggag cgtacggatt tctatgtccg gggagtggaa attccgcttc
     3721 ctcgatcgga cagactcgcg gcgttgccgc tccctaatcc gatctgcggc gagcggttcc
     3781 tgctctttca aaagcgatat ccagatcctg atttcgtcaa gggcgagcgg agcgagtgca
     3841 tttcaccctt gacgacgcac aaataaaaca ggcttggcag tggtgggagg tgacgcggag
     3901 cgacgccttc cacctgccag accgccgttg attttgacct tctggcagct tgctgcccga
     3961 acgattttgc gaagcaaagt ctagtgagta caccttctga aagatggtgg cctgtcagag
     4021 acggttttga tgttcgggaa ggtcaactgc aagggcggct taacaggtgg atggctcact
     4081 ttgttcacca cccacctttg ccaagtgttg gggtcgttgc agcccgtcaa gggtacgcat
     4141 acgccgctaa cgcgcccctg actggctttc actccggctc tgagcttccg aagcaggcct
     4201 gtagacgcct tttgattaca ccttcgctgt ttactagcgt tttggtaacg aaggctgcat
     4261 atggctttga tgtgttatac cccttccaaa cttcggcggg atctagggcg tatgcgcaga
     4321 tcttcccagc aatagggatt ttcactaaac agttgttttc ttccaagaaa ctaacccaat
     4381 tccttatcgt tctggttgtt acggaaagct ggtccgctaa gaattgttgg tctgctacaa
     4441 ctgctccgca gttgccatcg atgtgttctg ataggacgga atacaaccta gcagctcctg
     4501 gattgctctg tatgaggttt ctaatcctct cccatccctt tgggtaggtt tgagtgaaat
     4561 tcgtgttctt acgctcttct aggagctcct gttgcagttt tctgttttgc tcttccaagt
     4621 ctaagagtct ttgtttgatt tgagtctctg taattacttc ttgtgttccc atcagctccc
     4681 cctcgaattt ccacttaacc ggaatttatg tgtattttaa gtgttgtttc atttcctgaa
     4741 tatcaaatat aacttcccat gttcggaaag tataggaaat acagggtttc cggttaaccg
     4801 gaaatacagg gtttcctata ctatttctga aaacacatat aaatcagtaa gttatagaat
     4861 attcccttct tatctaaaag atcccactgc tcgcagcggg atcttgaagt agctcctccc
     4921 ttcggtcggg catttttggg gtcaaggttc gccatcggcg accgcgttag cggtttaacc
     4981 ttgacgctaa aaatgaccta ccaaacttgt cgagggtgat ggttcgcata gcgagccatc
     5041 gccctgcaag acggttttga ttttgaggtt tcggggcgta gccctgaacc agcacagaga
     5101 cccctctgtg tatgctggct taatgcagtt acgagcgatt ttgcgtagca aagtctagtg
     5161 agtacactat catcctgata gtggcccgcc ggaggcatcg caatgcgatc ccccgactgg
     5221 ccggatggac gccggaacga gggggtaagc atggcgaatt acgcgataat gcgctgcaaa
     5281 aagctgacgg gaatgggcag tgtggccagc gcgttgcagc actgctacag ggagcgtgag
     5341 acgccgaatg ctgacgctga acggacacca gaaaactact gctctgtgtc tcagtcggca
     5401 gatgaggcga tgggaaagct gcgggagctg ttgccggaaa aacgccgtaa agatgcggtg
     5461 ctggctgttg aatacgttat gacggcaagc cctgagtggt ggaacgaggc cacaccgcgg
     5521 cagcaggcgg agtttttcgc ccgctctgag caatggctgg agaagaaata cgggaaggat
     5581 cgtgttgtgg cggcggtggt tcaccgggac gaggccaccc cgcacctgtc tgcgttcgtg
     5641 gtgccattga cgcaggatgg acggctgtcc gcgaaagagt ttattggcgg gcggtcaaag
     5701 atgcgtgaag atcagagcac ctacgctgaa tcagtaaaaa aactgggtct tgagcgcggc
     5761 atcgagggaa gccgcgccac gcatcagaca gttcagcact actacgaatc gataaaccgt
     5821 ggcacccgta gccaggtatc gatctcgccg gaggcgctgg agccgcgtgt attgcggaag
     5881 ggcattttca ccaaagacgt ggaagatcag gcagccatag ccaaacgtct aagccacgca
     5941 gtgaacgatg gctttgcagg aaccatcgct atggcctctc agagcgccca gaacgcaaaa
     6001 agagcgcgtg agctacagaa gactatggat gcgcagcaga aacgcctaca gagcgttaca
     6061 gagcctttta aggggctttc cagagagcag atgaccgaaa tcctgatgat ggctcagcgg
     6121 tttaagcagc aaaaccagga aaaggaaaaa cagcagcgta ttgaacgtga aaaacagcga
     6181 cagacgcgat cgcgtggcat gggtggtatg gagcgttagt tttgatgcga tgcaggatag
     6241 ggctaacgcc gcaaagtgac gtcatttgcg gcattccaga ttaggttctg ccgccctgta
     6301 taacgctgag gccgtttttt agtgctttct tgtggttgct gacagtacct ttccagccaa
     6361 actgagggtt cagacggtaa ctgtaactgc gccctacttt tgggccttga agaatcacgg
     6421 ataggtcaat tagcgcctta accgaacggc ttacattggt tttctgcata cctaaagcgt
     6481 cggcaatatc ggcttgggca atttggatgt aattttcata atcgagatca gctaacagca
     6541 tcatcaagac tttgaattgc tcccctgtga gggtcttagc aaggatcttt aaagcgtctt
     6601 ggttcatagt gaaatgcctc tcaaaagctg acttctgttt gggtcggata actgcgacaa
     6661 aactacctac ctcttcccct gtagcagtgt ctatctgagt aattcggtta ctcatttaag
     6721 aggctctccc aaaaaaatag ttatcaataa tgataacaac gttatcaata atgataacta
     6781 tcgtcaagcg agagccgcgc cccacaaggg ctgagagaag gctctcttct gtatcttata
     6841 gcgagcgtag cgagtgccat acctaaagta cgattagtac ttgagatgag tacgattagt
     6901 acttgaggtg agtacgatta gtacttgagg tgagtacgat tagtacttga ggtgagtacg
     6961 attcttctct ctagcccttg gtgtgcgcgg gttcccgtat ctatatctat atattaaggg
     7021 ggaaataaag gctgaaaaac gccattttta aacatcgaaa aacgagcttt tttgagtctg
     7081 gagcgtacgg atttctatgt ccggggagtg gaaattccgc ttcctcgatc ggacagactc
     7141 gcggcgttgc cgctccctaa tccgatctgc ggcgagcggt tcctgctctt tcaaaagcga
     7201 tatccagatc ctgatttcgt caagggcgag cggagcgagt gcatttcacc cttgacgacg
     7261 cacaaataaa acaggcttgg cagtggtggg aggtgacgcg gagcgacgcc ttccacctgc
     7321 cagaccgccg ttgattttga ccttctggca gcttgctgcc cgaacgattt tgcgaagcaa
     7381 agtctagtga gtacaccttc tgaaagatgg tggcctgtca gagacggttt tgatgttcgg
     7441 gaaggtcaac tgcaagggcg gcttaacagg tggatggctc actttgttca ccacccacct
     7501 ttgccaagtg ttggggtcgt tgcagcccgt caagggtacg catacgccgc taacgcgccc
     7561 ctgactggct ttcactccgg ctctgagctt ccgaagcagg cctgtagacg ccttttgatt
     7621 acaccttcgc tgtttactag cgttttggta acgaaggctg catatggctt tgatgtgtta
     7681 taccccttcc aaacttcggc gggatctagg gcgtatgcgc agatcttccc agcaataggg
     7741 attttcacta aacagttgtt ttcttccaag aaactaaccc aattccttat cgttctggtt
     7801 gttacggaaa gctggtccgc taagaattgt tggtctgcta caactgctcc gcagttgcca
     7861 tcgatgtgtt ctgataggac ggaatacaac ctagcagctc ctggattgct ctgtatgagg
     7921 tttctaatcc tctcccatcc ctttgggtag gtttgagtga aattcgtgtt cttacgctct
     7981 tctaggagct cctgttgcag ttttctgttt tgctcttcca agtctaagag tctttgtttg
     8041 atttgagtct ctgtaattac ttcttgtgtt cccatcagct ccccctcgaa tttccactta
     8101 accggaattt atgtgtattt taagtgttgt ttcatttcct gaatatcaaa tataacttcc
     8161 catgttcgga aagtatagga aatacagggt ttccggttaa ccggaaatac agggtttcct
     8221 atactatttc tgaaaacaca tataaatcag taagttatag aatattccct tcttatctaa
     8281 aagatcccac tgctcgcagc gggatcttga agtagctccc tcccttcggt cgggcatttt
     8341 tggggtcaag gttcgccatc ggcgaccgcg ttagcggttt aaccttgacg ctaaaaatga
     8401 cctaccaaac ttgtcgaggg tgatggttcg catagcgagc catcgccctg caagacggtt
     8461 ttgattttga ggtttcgggg cgtagccctg aaccagcaca gagacccctc tgtgtatgct
     8521 ggcttaatgc agttacgagc gattttgcgt agcaaagtct agtgagtaca ctatcatcct
     8581 gatagtggcc cgccggaggc atcgcaatgc gatcccccga ctggccggat ggacgccgga
     8641 acgagggggt aagcatggcg aattacgcga taatgcgctg caaaaagctg acgggaatgg
     8701 gcagtgtggc cagcgcgttg cagcactgct acagggagcg tgagacgccg aatgctgacg
     8761 ctgaacggac accagaaaac tactgctctg tgtctcagtc ggcagatgag gcgatgggaa
     8821 ctgcgggagc tgttgccgga aaaacgccgt aaagatgcgg tgctggctgt tgaatacgtt
     8881 atgacggcaa gccctgagtg gtggaacgag gcc
//
