LOCUS       CP077067                9982 bp    DNA     circular BCT 27-JUN-2021
DEFINITION  Escherichia coli strain Q4552 plasmid pECQ4552_IHU04, complete
            sequence.
ACCESSION   CP077067
VERSION     CP077067.1
DBLINK      BioProject: PRJNA738695
            BioSample: SAMN19756648
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9982)
  AUTHORS   Hamame,A., Davoust,B., Hamel,M., Rolain,J.-M. and Diene,S.M.
  TITLE     Genomic characterization of a mcr-1 and mcr-3-producing Escherichia
            coli strain isolated from pig in France
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9982)
  AUTHORS   Hamame,A., Davoust,B., Hamel,M., Rolain,J.-M. and Diene,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2021) Aix-Marseille University, MEPHI, IRD, APHM,
            IHU-Mediterranee Infection, Marseille, France, 19-21 Bd Jean Moulin,
            Marseille 13005, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2021
            Assembly Method                   :: Canu v. 1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 34.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/21/2021 12:56:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,833
            CDSs (total)                      :: 4,716
            Genes (coding)                    :: 3,694
            CDSs (with protein)               :: 3,694
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 1,022
            CDSs (without protein)            :: 1,022
            Pseudo Genes (ambiguous residues) :: 0 of 1,022
            Pseudo Genes (frameshifted)       :: 875 of 1,022
            Pseudo Genes (incomplete)         :: 170 of 1,022
            Pseudo Genes (internal stop)      :: 38 of 1,022
            Pseudo Genes (multiple problems)  :: 59 of 1,022
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from MEPHI, IHU-Mediterranee
            infection, 19-21 Bd Jean moulin 13005, Marseille.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Q4552"
                     /isolation_source="pig"
                     /db_xref="taxon:562"
                     /plasmid="pECQ4552_IHU04"
                     /geo_loc_name="France: Avignon"
                     /lat_lon="43.85274931 N 4.96206883 E"
                     /collection_date="2020-01"
                     /collected_by="Afaf Hamame"
     gene            complement(join(9816..9982,1))
                     /locus_tag="KRX18_23970"
                     /pseudo=""
     CDS             complement(join(9816..9982,1))
                     /locus_tag="KRX18_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /name="replication protein C"
     gene            complement(join(9982,1..1205))
                     /locus_tag="KRX18_23975"
                     /pseudo=""
     CDS             complement(join(9982,1..1205))
                     /locus_tag="KRX18_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            1404..1688
                     /locus_tag="KRX18_23980"
     CDS             1404..1688
                     /locus_tag="KRX18_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="QWZ14455.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            complement(2356..3209)
                     /gene="estX"
                     /locus_tag="KRX18_23985"
                     /pseudo=""
     CDS             complement(2356..3209)
                     /gene="estX"
                     /locus_tag="KRX18_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /name="estX"
     gene            complement(3359..4273)
                     /locus_tag="KRX18_23990"
     CDS             complement(3359..4273)
                     /locus_tag="KRX18_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C"
                     /protein_id="QWZ14457.1"
                     /translation="MTAGCSSPPCWRGSARARGCPVVKPKNKHSLSHVRHDPAHCLAPG
                     LFRALKRGERKRSKLDVTYDYGDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGP
                     EPKTEGGRQLRLFLEPKWEAVTADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERL
                     WKVSIIAQNGRKRQGFRLLSEYASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRA
                     LDSETARLLHQRLCGWIDPGKTGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELV
                     ALGWTVTEFAAGKYDITRPKAAG"
                     /name="replication protein C"
     gene            complement(4197..5036)
                     /locus_tag="KRX18_23995"
     CDS             complement(4197..5036)
                     /locus_tag="KRX18_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="QWZ14456.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(5066..5272)
                     /locus_tag="KRX18_24000"
     CDS             complement(5066..5272)
                     /locus_tag="KRX18_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWZ14451.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            complement(5274..5486)
                     /locus_tag="KRX18_24005"
     CDS             complement(5274..5486)
                     /locus_tag="KRX18_24005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QWZ14452.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(5547..7676)
                     /locus_tag="KRX18_24010"
     CDS             complement(5547..7676)
                     /locus_tag="KRX18_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="QWZ14453.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="MobA/MobL family protein"
     gene            7876..8160
                     /locus_tag="KRX18_24015"
     CDS             7876..8160
                     /locus_tag="KRX18_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="QWZ14454.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            complement(8813..9666)
                     /gene="estX"
                     /locus_tag="KRX18_24020"
                     /pseudo=""
     CDS             complement(8813..9666)
                     /gene="estX"
                     /locus_tag="KRX18_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /name="estX"
     Typing          complement(1295..1450)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(7767..7922)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(4198..5019)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(4..1205)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5551..7676)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccttgagct gtcggcctat ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc
       61 gcagccagat cgagccgtcc tcggttgtca gtggcgtcag gtcgagcaag agcaacgatg
      121 cgatcagcag caccaccgta ggcatcatgg aagccagcat cacggttagc catagcttcc
      181 agtgccaccc ccgcgacgcg ctccgggcgc tctgcgcggc gctgctcacc tcggcggcta
      241 cctcccgcaa ctctttggcc agctccaccc atgccgcccc tgtctggcgc tgggctttca
      301 gccactccgc cgcctgcgcc tcgctggcct gctgggtctg gctcatgacc tgccgggctt
      361 cgtcggccag tgtcgccatg ctctgggcca gcggttcgat ctgctccgct aactcgttga
      421 tgcctctgga tttcttcact ctgtcgattg cgttcatggt ctattgcctc ccggtattcc
      481 tgtaagtcga tgatctgggc gttggcggtg tcgatgttca gggccacgtc tgcccggtcg
      541 gtgcggatgc cccggccttc catctccacc acgttcggcc ccaggtgaac accgggcagg
      601 cgctcgatgc cctgcgcctc aagtgttctg tggtcaatgc gggcgtcgtg gccagcccgc
      661 tctaatgccc ggttggcatg gtcggcccat gcctcgcggg tctgctcaag ccatgccttg
      721 ggcttgagcg cttcggtctt ctgtgccccg cccttctccg gggtcttgcc gttgtaccgc
      781 ttgaaccact gagcggcggg ccgctcgatg ccgtcattga tccgctcgga gatcatcagg
      841 tggcagtgcg ggttctcgcc gccaccggca tggatggcca gcgtatacgg caggcgctcg
      901 gcaccagtca ggtgctgggc gaactcggac gccagcgcct tctgctggtc gagggtcagc
      961 tcgaccggca gggcaaattc gacctccttg aacagccgcc cattggcgcg ttcatacagg
     1021 tcggcagcat cccagtagtc ggcgggccgc tcgacgaact ccggcatgtg cccggattcg
     1081 gcgtgcaaga cttcatccat gtcgcgggca tacttgcctt cgcgctggat gtagtcggcc
     1141 ttggccctgg ccgattggcc gcccgacctg ctgccggttt tcgccgtaag gtgataaatc
     1201 gccatgctgc ctcgctgttg cttttgcttt tcggctccat gcaatggccc tcggagagcg
     1261 caccgcccga agggtggccg ttaggccagt ttctcgaaga gaaaccggta agtgcgccct
     1321 cccctacaaa gtagggtcgg gattgccgcc gctgtgcctc catgatagcc tacgagacag
     1381 cacattaaca atggggtgtc aagatggtta aggggagcaa caaggcggcg gatcggctgg
     1441 ccaagctcga agaacaacga gcgcgaatca atgccgaaat tcagcgggtg cgggcaaggg
     1501 aacagcagca agagcgcaag aacgaaacaa ggcgcaaggt gctggtgggg gccatgattt
     1561 tggccaaggt gaacagcagc gagtggccgg aggatcggct catggcggca atggatgcgt
     1621 accttgaacg cgaccacgac cgcgccttgt tcggtctgcc gccacgccag aaggatgagc
     1681 cgggctgaat gatcgaccga gacaggccct gcggggctgc acacgcgccc ccacccttcg
     1741 ggtaggggga aaggccgcta aagcggctaa aagcgctcca gcgtatttct gcggggtttg
     1801 gtgtggggtt tagcgggctt tgcccgcctt tcccctgccg cgcagcggtg gggcggtgtg
     1861 tagcctagcg cagcgaatag accagctatc cggcctctgg ccgggcatat tgggcaaggg
     1921 cagcagcgcc ccacaagggc gctgataacc gcgcctagtg gattattctt agataatcat
     1981 ggatggattt ttccaacacc ccgccagccc ccgcccctgc tgggtttgca ggtttggggg
     2041 cgtgacagtt attgcagggg ttcgtgacag ttattgcagg gggcgtgaca gttattgcag
     2101 gggttcgtga cagttagtac gggagtgacg ggcactggct ggcaatgtct agcaacggca
     2161 ggcatttcgg ctgagggtaa aagaactttc cgctaagcga tagactgtat gtaaacacag
     2221 tattgcaagg acgcggaaca tgcctcagcg atcaccgctt ccctcatgat gtttaacgcc
     2281 ttgctcaccg gaaaattgcg agcgcagcga gtaattttta cggtgcagca acttgttgta
     2341 cggtgcaacc gatagctatg acgtttgccc cttaatcgcc tggataatcc tcggccagtc
     2401 ttcatgatgc aactcatgtc ccgtgccttc gagtgtcagc atttttgaac cacgaatcgc
     2461 atctttcagt gccaacccat gcacataagg aagtacagga tcctccgtgc cgtgaatgat
     2521 caaagttggt acagctatct catttaatct cccgagccat ctctcgagcg atctctcgcc
     2581 accacctgaa gtagtgtggt tgaatgttgt caggatattc ggagtgcgat caaaatctaa
     2641 ctcagcgata ttttgtatct tctcagcgtc aaaagcatgc gcagtacctg agatgattcg
     2701 ccacgctccg acctgatacg cgacgacagc atctctatca gaccaatcca gcgattccgc
     2761 ccgttggtga tactcaatga tggcaggatc aaaagcgggc atatcaggat ctgcatctgc
     2821 aagccgttct gaagcaatca gcgtcaagct cttcacacgt ttcggatact tgagagctac
     2881 aagctgggaa agaaatcccc caaagacatg ccgactaaat gagcagcttc cagaccataa
     2941 ccatcaatga cgcgaaccac atcatctgct aattcttcaa cggaatatgg agcttgacct
     3001 ggctcatagc ttgttgattt cccggtatca cggtggtcgt accggatcac atagcgaccc
     3061 attttggcta gttgggagca aaactcatca ggccaccaca ccgcagacgc cattgccccc
     3121 atgatcagga taatgggttc atgggccggg tcgccgaatg attcggtatg gagtgaaatc
     3181 gctttatcaa caactacctt ttctttcata ctaaaattgc cttcgagttt tacgtacaac
     3241 attttattca taggcatgct cattttgcca gttttggctg cctgttaatt gcgctcaaac
     3301 atccccaacc aattgtttta taaagataaa aatgtcttgt ttacaataga gtggggggtc
     3361 agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc cgcgaactcg gttaccgtcc
     3421 agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg ctggcggcgc ttgcgcatgg
     3481 tcgaaccact ggcctctgac ggccagacat agccgcacaa ggtatctatg gaagccttgc
     3541 cggttttgcc ggggtcgatc cagccacaca gccgctggtg cagcaggcgg gcggtttcgc
     3601 tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg ctggccgcca cccatgacgg
     3661 cctgcgcgat caaggggttc agggccacgt acaggcgccc gtccgcctcg tcgctggcgt
     3721 actccgacag cagccgaaac ccctgccgct tgcggccatt ctgggcgatg atggatacct
     3781 tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc cccaccacta tcgacctctg
     3841 ccccgatttc ctttgccagc gcccgatagc tacctttgac caccatggca tcagcggtga
     3901 cggcctccca cttgggttcc aggaacagcc ggagctgccg tccgccttcg gtcttgggtt
     3961 ccgggccaag cactaggcca ttaggcccag ccatggccac cagcccttgc aggatgcgca
     4021 gatcatcagc gcccagcggc tccgggccgc tgaactcgat ccgcttgccg tcgccgtagt
     4081 catacgtcac gtccagcttg ctgcgcttgc gctcgccccg cttgagggca cggaacaggc
     4141 cgggggccag acagtgcgcc gggtcgtgcc ggacgtggct gaggctgtgc ttgttcttag
     4201 gcttcaccac ggggcacccc cttgctcttg cgctgcctct ccagcacggc gggcttgagc
     4261 accccgccgt catgccgcct gaaccaccga tcagcgaacg gtgcgccata gttggccttg
     4321 ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt ccacacccca ttcctcggcc
     4381 tcggcgctgg tcatgctcga caggtaggac tgccagcgga tgttatcgac cagtaccgag
     4441 ctgccccggc tggcctgctg ctggtcgcct gcgcccatca tggccgcgcc cttgctggca
     4501 tggtgcagga acacgataga gcacccggta tcggcggcga tggcctccat gcgaccgatg
     4561 acctgggcca tggggccgct ggcgttttct tcctcgatgt ggaaccggcg cagcgtgtcc
     4621 agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc cgtcgaacca ctccggggcc
     4681 atgatgttgg gcaggctgcc gatcagcggc tggatcagca ggccgtcagc cacggcttgc
     4741 cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc ggtgatgaat ggcggtgggc
     4801 gggtcttcgg cgggcaggta gatcaccggg ccggtgggca gttcgcccac ctccagcaga
     4861 tccggcccgc ctgcaatctg tgcggccagt tgcagggcca gcatggattt accggcacca
     4921 ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt tgggcaaaac gtagtccagc
     4981 ggtggcggcg ctgctgcgaa cgcctccaga atattgatag gcttatgggt agccattgat
     5041 tgcctccttt gcaggcagtt ggtggttagg cgctggcggg gtcactaccc ccgccctgcg
     5101 ccgctctgag ttcttccagg cactcgcgca gcgcctcgta ttcgtcgtcg gtcagccaga
     5161 acttgcgctg acgcatccct ttggccttca tgcgctcggc atatcgcgct tggcgtacag
     5221 cgtcagggct ggccagcagg tcgccggtct gcttgtcctt ttggtctttc atatcagtca
     5281 ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga acaaggacaa ggtgcagccg
     5341 tcaaggttaa ggctggccat atcagcgact gaaaagcggc cagcctcggc cttgtttgac
     5401 gtataaccaa agccaccggg caaccaatag cccttgtcac ttttgatcag gtagaccgac
     5461 cctgaagcgc ttttttcgta ttccataaaa cccccttctg tgcgtgagta ctcatagtat
     5521 aacaggcgtg agtaccaacg caagcactac atgctgaaat ctggcccgcc cctgtccatg
     5581 cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc gcgcaagctg gacgctgggc
     5641 agacccatga ccttgctgac ggtgcgctcg atgtaatccg cttcgtggcc gggcttgcgc
     5701 tctgccagcg ctgggctggc ctcggccatg gccttgccga tttcctcggc actgcggccc
     5761 cggctggcca gcttctgcgc ggcgataaag tcgcacttgc tgaggtcatc accgaagcgc
     5821 ttgaccagcc cggccatctc gctgcggtac tcgtccagcg ccgtgcgccg gtggcggcta
     5881 agctgccgct cgggcagttc gaggctggcc agcctgcggg ccttctcctg ctgccgctgg
     5941 gcctgctcga tctgctggcc agcctgctgc accagcgccg ggccagcggt ggcggtcttg
     6001 cccttggatt cacgcagcag cacccacggc tgataaccgg cgcgggtggt gtgcttgtcc
     6061 ttgcggttgg tgaagcccgc caagcggcca tagtggcggc tgtcggcgct ggccgggtcg
     6121 gcgtcgtact cgctggccag cgtccgggca atctgccccc gaagttcacc gcctgcggcg
     6181 tcggccacct tgacccatgc ctgatagttc ttcgggctgg tttccactac cagggcaggc
     6241 tcccggccct cggctttcat gtcatccagg tcaaactcgc tgaggtcgtc caccagcacc
     6301 agaccatgcc gctcctgctc ggcgggcctg atatacacgt cattgccctg ggcattcatc
     6361 cgcttgagcc atggcgtgtt ctggagcact tcggcggctg accattcccg gttcatcatc
     6421 tggccggtgg tggcgtccct gacgccgata tcgaagcgct cacagcccat ggccttgagc
     6481 tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg ggccaccaag cgcagccaga
     6541 tcgagccgtc ctcggttgtc agtggcgtca ggtcgagcaa gagcaacgat gcgatcagca
     6601 gcaccaccgt aggcatcatg gaagccagca tcacggttag ccatagcttc cagtgccacc
     6661 cccgcgacgc gctccgggcg ctctgcgcgg cgctgctcac ctcggcggct acctcccgca
     6721 actctttggc cagctccacc catgccgccc ctgtctggcg ctgggctttc agccactccg
     6781 ccgcctgcgc ctcgctggcc tgctgggtct ggctcatgac ctgccgggct tcgtcggcca
     6841 gtgtcgccat gctctgggcc agcggttcga tctgctccgc taactcgttg atgcctctgg
     6901 atttcttcac tctgtcgatt gcgttcatgg tctattgcct cccggtattc ctgtaagtcg
     6961 atgatctggg cgttggcggt gtcgatgttc agggccacgt ctgcccggtc ggtgcggatg
     7021 ccccggcctt ccatctccac cacgttcggc cccaggtgaa caccgggcag gcgctcgatg
     7081 ccctgcgcct caagtgttct gtggtcaatg cgggcgtcgt ggccagcccg ctctaatgcc
     7141 cggttggcat ggtcggccca tgcctcgcgg gtctgctcaa gccatgcctt gggcttgagc
     7201 gcttcggtct tctgtgcccc gcccttctcc ggggtcttgc cgttgtaccg cttgaaccac
     7261 tgagcggcgg gccgctcgat gccgtcattg atccgctcgg agatcatcag gtggcagtgc
     7321 gggttctcgc cgccaccggc atggatggcc agcgtatacg gcaggcgctc ggcaccagtc
     7381 aggtgctggg cgaactcgga cgccagcgcc ttctgctggt cgagggtcag ctcgaccggc
     7441 agggcaaatt cgacctcctt gaacagccgc ccattggcgc gttcatacag gtcggcagca
     7501 tcccagtagt cggcgggccg ctcgacgaac tccggcatgt gcccggattc ggcgtgcaag
     7561 acttcatcca tgtcgcgggc atacttgcct tcgcgctgga tgtagtcggc cttggccctg
     7621 gccgattggc cgcccgacct gctgccggtt ttcgccgtaa ggtgataaat cgccatgctg
     7681 cctcgctgtt gcttttgctt ttcggctcca tgcaatggcc tctcggagag cgcaccgccc
     7741 gaagggtggc cgttaggcca gtttctcgaa gagaaaccgg taagtgcgcc ctcccctaca
     7801 aagtagggtc gggattgccg ccgctgtgcc tccatgatag cctacgagac agcacattaa
     7861 caatggggtg tcaagatggt taaggggagc aacaaggcgg cggatcggct ggccaagctc
     7921 gaagaacaac gagcgcgaat caatgccgaa attcagcggg tgcgggcaag ggaacagcag
     7981 caagagcgca agaacgaaac aaggcgcaag gtgctggtgg gggccatgat tttggccaag
     8041 gtgaacagca gcgagtggcc ggaggatcgg ctcatggcgg caatggatgc gtaccttgaa
     8101 cgcgaccacg accgcgcctt gttcggtctg ccgccacgcc agaaggatga gccgggctga
     8161 atgatcgacc gagacaggcc ctgcggggct gcacacgccg ggtaggggaa aggccgctaa
     8221 agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt agcgggcttt
     8281 gcccgccttt ccctgccgcg cagcggtggg gcggtgtgta gcctagcgca gcgaatagac
     8341 cagctatccg gcctctggcc gggcatattg ggcaagggca gcagcgcccc acaagggcgc
     8401 tgataaccgc gcctagtgga ttattcttag ataatcatgg atggattttt ccaacacccc
     8461 gccagccccc gcccctgctg ggtttgcagg tttgggggcg tgacagttat tgcaggggtt
     8521 cgtgacagtt attgcagggg gcgtgacagt tattgcaggg gttcgtgaca gttagtacgg
     8581 gagtgacggg cactggctgg caatgtctag caacggcagg catttcggct gagggtaaaa
     8641 gaactttccg ctaagcgata gactgtatgt aaacacagta ttgcaaggac gcggaacatg
     8701 cctcagcgat caccgcttcc ctcatgatgt ttaacgcctt gctcaccgga aaattgcgag
     8761 cgcagcgata atttttacgg tgcagcaact tgttgtacgg tgcaaccgat agctatgacg
     8821 tttgcccctt aatcgcctgg ataatcctcg gccagtcttc atgatgcaac tcatgtcccg
     8881 tgccttcgag tgtcagcatt tttgaaccac gaatcgcatc tttcagtgcc aacccatgca
     8941 cataaggaag tacaggatcc tccgtgccgt gaatgatcaa agttggtaca gctatctcat
     9001 ttaatctccc gagccatctc tcgagcgatc tctcgccacc acctgaagta gtgtggttga
     9061 atgttgtcag gatattcgga gtgcgatcaa aatctaactc agcgatattt tgtatcttct
     9121 cagcgtcaaa agcatgcgca gtacctgaga tgattcgcca cgctccgacc tgatacgcga
     9181 cgacagcatc tctatcagac caatccagcg attccgcccg ttggtgatac tcaatgatgg
     9241 caggatcaaa agcgggcata tcaggatctg catctgcaag ccgttctgaa gcaatcagcg
     9301 tcaagctctt cacacgtttc ggatacttga gagctacaag ctgggaaaga aatcccccaa
     9361 agacatgccg actaaatgag cagcttccag accataacca tcaatgacgc gaaccacatc
     9421 atctgctaat tcttcaacgg aatatggagc ttgacctggc tcatagcttg ttgatttccc
     9481 ggtatcacgg tggtcgtacc ggatcacata gcgacccatt ttggctagtt gggagcaaaa
     9541 ctcatcaggc caccacaccg cagacgccat tgcccccatg atcaggataa tgggttcatg
     9601 ggccgggtcg ccgaatgatt cggtatggag tgaaatcgct ttatcaacaa ctaccttttc
     9661 tttcatacta aaattgcctt cgagttttac gtacaacatt ttattcatag gcatgctcat
     9721 tttgccagtt ttggctgcct gttaattgcg ctcaaacatc cccaaccaat tgttttataa
     9781 agataaaaat gtcttgttta caatagagtg gggggtcagc ctgccgcctt gggccgggtg
     9841 atgtcgtacc ccgccgcgaa ctcggttacc gtccagccca gcgcgaccag ctccggcaac
     9901 gcctcgcgca cccgctggcg gcgcttgcgc atggtcgaac cactggcctc tgacggccag
     9961 acatagccgc acaaggtatc ta
//
