LOCUS       CP076400               10486 bp    DNA     circular BCT 21-FEB-2023
DEFINITION  Acinetobacter nosocomialis strain 2S8-227 plasmid p2S8-227-11k,
            complete sequence.
ACCESSION   CP076400
VERSION     CP076400.1
DBLINK      BioProject: PRJNA734730
            BioSample: SAMN19522711
KEYWORDS    .
SOURCE      Acinetobacter nosocomialis
  ORGANISM  Acinetobacter nosocomialis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Huang,T.-W. and Lauderdale,T.-L.Y.
  TITLE     Genome analysis of Colistin-resistant Acinetobacter nosocomialis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Huang,T.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2021) Department of Microbiology and Immunology,
            School of Medicine, College of Medicine, Taipei Medical University,
            No. 250, Wuxin Street, Taipei 11031, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2020
            Assembly Method                   :: Unicycler v. 2020-08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 440.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/04/2021 17:42:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,165
            Genes (coding)                    :: 3,283
            CDSs (with protein)               :: 3,283
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 882
            CDSs (without protein)            :: 882
            Pseudo Genes (ambiguous residues) :: 0 of 882
            Pseudo Genes (frameshifted)       :: 748 of 882
            Pseudo Genes (incomplete)         :: 115 of 882
            Pseudo Genes (internal stop)      :: 49 of 882
            Pseudo Genes (multiple problems)  :: 29 of 882
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Acinetobacter nosocomialis"
                     /mol_type="genomic DNA"
                     /strain="2S8-227"
                     /host="Homo sapiens"
                     /db_xref="taxon:106654"
                     /plasmid="p2S8-227-11k"
                     /geo_loc_name="Taiwan"
                     /collection_date="2012"
                     /collected_by="National Health Research Institutes"
     gene            complement(146..1095)
                     /locus_tag="KNV99_21230"
                     /pseudo=""
     CDS             complement(146..1095)
                     /locus_tag="KNV99_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002058606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /name="GGDEF domain-containing protein"
     gene            1354..1497
                     /locus_tag="KNV99_21235"
                     /pseudo=""
     CDS             1354..1497
                     /locus_tag="KNV99_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985693.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(2026..2571)
                     /locus_tag="KNV99_21240"
     CDS             complement(2026..2571)
                     /locus_tag="KNV99_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WDM41743.1"
                     /translation="MKALSVIELSQLYGMNRQSIYKRINKGDLSKNSDGKIDLSEAIRV
                     FGEPSNKNNNVTTLQSTTVQKDTEVDMLKQQVDILKKQLELAHEREVFQREQLEAKDNQ
                     IEAIQRLLEAPKTNMTTFTDQKPSQDIVTNPRPESEPKHDVLTTPGQTENKRIPVPEHI
                     EQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(2640..3575)
                     /gene="repM"
                     /locus_tag="KNV99_21245"
     CDS             complement(2640..3575)
                     /gene="repM"
                     /locus_tag="KNV99_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WDM41744.1"
                     /translation="MPTSELIVKDNALINASYNLDTVEQRLILLAILQARETRTGIDAN
                     TRLRIHASDYMSRFNVNKHAAYKALKTAVTNLFGRQFSYQTIDEKTGKSKKVISRWVQN
                     ISYIDDAATLEVTFTMDVVPLITRLEKQFTSYQLKQVTQLTGKYAIRLYELLIAWREVG
                     KTPIFEVSDFRSKLGLTSDDYPRLDTFKRRVLESAVKQINEHTDIIVKVEQHKEGRSIS
                     GFSFSFKQKRNPNKVIERKKDPNTLDIFSRITDAQRHLFANKLSELPEMGKYSQGTESY
                     QQFAIRIAEMLQDPAKFQELHPYLEKVGYR"
                     /name="repM"
     gene            complement(4140..5314)
                     /locus_tag="KNV99_21250"
                     /pseudo=""
     CDS             complement(4140..5314)
                     /locus_tag="KNV99_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            5618..6379
                     /locus_tag="KNV99_21255"
     CDS             5618..6379
                     /locus_tag="KNV99_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41733.1"
                     /translation="MTQEKAKKRGRPSQLLQMAELHAFVEFLLEKDPRSELQNQVIDAL
                     QAEDFNFEMLSEAQQILVKEALKPYREHLKLQLLFDELSQNFQHTEYEEKFMELYQGYK
                     QDGLDPVEMNILKMMCTRYLNFKAQKLEYSDLELYLGQIQKKNASEKRKAENKRKFELG
                     GAVLAAFKKLNLDISKETPEQITNRIVNNKVFHDEVKKSKIFSEVSKYKKEYFERNKLF
                     IDVLEGLHTWKKEGMTLTQIEIEKVLRKNNE"
                     /name="hypothetical protein"
     gene            complement(6546..6788)
                     /locus_tag="KNV99_21260"
     CDS             complement(6546..6788)
                     /locus_tag="KNV99_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41734.1"
                     /translation="MNYFTQPQLFISAKAKKLGLQLIQELYIQRSTRLAFYLDVNPSFD
                     GHEAVLFWTEHYLAEYPDANFSDIQQQFIQAVPQS"
                     /name="hypothetical protein"
     gene            6941..7186
                     /locus_tag="KNV99_21265"
     CDS             6941..7186
                     /locus_tag="KNV99_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WDM41735.1"
                     /translation="MPYEEFHRLIGKAGLSIKEFAELLGMRPNSITNYNKVGFVPSHLA
                     VIVSLISTMKDEGFDFYPILEKVQAYEKARIESQSK"
                     /name="helix-turn-helix domain-containing protein"
     gene            7494..8192
                     /locus_tag="KNV99_21270"
     CDS             7494..8192
                     /locus_tag="KNV99_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41736.1"
                     /translation="MERNLNLANSIFEGFNDKNGLMICGYEWGWSKEDQKLDESDSRPT
                     VDMSIECTFSNKSLRYGPSSNTWPYDKNIKKWFNFWGHPLNDENLGSDFDKCIIQTNWA
                     YTSNADIESYNRFLEKEAVDNFIHHIEVLRPKVILFMGSKLINFLRNIDVWDRFTAIVG
                     PEIEPLKMVQKTDFDGTRFKVYFDNFEQCQVVCLPHPSSSRGLSDEYIKLFEPELGTII
                     TEYKKQKGIL"
                     /name="hypothetical protein"
     gene            8201..8752
                     /locus_tag="KNV99_21275"
     CDS             8201..8752
                     /locus_tag="KNV99_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="WDM41737.1"
                     /translation="MKKVHLIQADITAFAVHAIVNSANKSLLGGGGLDYVIHKKAGPLM
                     KEECVRLNQEKGGCPTGQAEVTTAGNLPAKYLIHAVGPRWLDGEHNEPQLLCDAYSNAL
                     FKANEIHALTVSFPCISTGVYGFPPQKAAEIAIGTILSMLPQYDHVAEVFFICREDENY
                     LIYKNLLSNIDDPNIQILIS"
                     /name="macro domain-containing protein"
     gene            complement(8754..9140)
                     /locus_tag="KNV99_21280"
     CDS             complement(8754..9140)
                     /locus_tag="KNV99_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WDM41738.1"
                     /translation="MSLISSTFVIAVHDEILLQTGVGRIGCHIEKLESVLSRIDQQMYY
                     SNVSDIFEIAAWFGIAISKGHAFVDGNKRTGLAVMLTYLEIQGVTIQEQTGLDDLMVDI
                     VESQEEHELLAKIVAEFLYELSII"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(9137..9316)
                     /locus_tag="KNV99_21285"
     CDS             complement(9137..9316)
                     /locus_tag="KNV99_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41739.1"
                     /translation="MRKRLKDYSLDQISNREKVSGQVLPIQFSNDEATKRVVMHSAKRV
                     INQHKAEIQELAYK"
                     /name="hypothetical protein"
     gene            complement(9366..9701)
                     /locus_tag="KNV99_21290"
     CDS             complement(9366..9701)
                     /locus_tag="KNV99_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41740.1"
                     /translation="MKIGIFWFLQKQVIGIAHPFNLNDADSIGLIDSPYTHVDYWKNMQ
                     SVYPELRHYEYEQIPRGRVVFDANKEKAIVYMDKKLFNTVTATKIYDFFDIDSENAIPR
                     KDPHYRT"
                     /name="hypothetical protein"
     gene            complement(9784..10098)
                     /locus_tag="KNV99_21295"
     CDS             complement(9784..10098)
                     /locus_tag="KNV99_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WDM41741.1"
                     /translation="MSMVRYSRKELNEKFSDKQDAEIQRLLAKGTVPDDQLDLSDMPEI
                     TDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(10085..10372)
                     /locus_tag="KNV99_21300"
     CDS             complement(10085..10372)
                     /locus_tag="KNV99_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WDM41742.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDG
                     EERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERDRYEHG"
                     /name="BrnT family toxin"
     Typing          complement(2641..3575)
                     /name="rep_cluster_1233"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.893"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4827..5314)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.411"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaagccgac ttgtttccaa gtcggctttt agggtttgcg tccccgattt tttataacat
       61 aactttttga aatataaaac tatttttata tgcgtttcgt ataacgccca ttatgttaat
      121 tgtaggaaaa cttaatttta ttaccttatt gagacatatc tttgatttga cgtttatgtc
      181 tatacatttg cttatctgct tcaactactg tttgttgtaa atccgtccct ggttgacgtg
      241 ctgcaacacc aatagcagca ttaattccag cctgttttaa ggattctgaa attcgatttg
      301 ctagtttttg agtattttct aagttagttt caatatttaa aataccaaac tcatctcctc
      361 ctaaacgtgc aactaaatca ttagagcgca cagttctttt gataatttgg actgtttctt
      421 gaatcatctg atcacctttt aaatgaccaa atttatcatt tatatatttt aaatcattta
      481 aatctatgac aataattgtt gttgagtgtc cgtaaatttt acaccgactt tcttctaaat
      541 ctaaaaattt atcccaagca cgacgattga aagctcctgt taagtgatct gttagcgact
      601 ccatttcaag tttttcgcta attctttttt gttcatttat tttaatttcg ttctgtaaaa
      661 ttcgactgag taataaacta aataattcta ataaatcact atattgttct atacttttag
      721 gctgtactga tggatcaata gcacataatg taccgaaaac agagccatct tcattcaata
      781 aaggttgacc tatatatgca tttatatcca ctaccttatt aattccagcc tcttcatata
      841 atggaatttt tttgaatcat gtgctatacg tggccctttg cctagtacca tcttagaaca
      901 atatgaatca gcccaattaa aaacttgtcc tgcttttact ccatatccac tgtctttagc
      961 ttgaagaact atccaatcat taccttctgt acaagtaatc atccataaat taaaaactaa
     1021 gtgattatgt aaatactcta aaatggcttt accagcatct tcaaaatctc taaagttttc
     1081 ataaaaaaga ctcataacta ctcactatca atactttaaa aatttataca actttattta
     1141 aactattgta tttaaagatt aatttaaaaa tttcatagat ataatatatc gtaaaattta
     1201 tttttcttaa tattttttac gatatattct taaacgactc tatatagaaa atataacttt
     1261 ttagttaaca acgtaagcgt ccgctgatcc ccactttttc taactcatta ataccgcgat
     1321 agtgtccact aaaatttaag tggacaccta tctatggatt taaagacagt tatagacact
     1381 gcaccaacgc taaaaaagcc ccgtcgaacc ttcacggctg aatttaaaca tcaacttatt
     1441 cagcaatgcc agcagccaga tacatcggtg gctaaggtcg caatgcaaca tcagatcaag
     1501 agatggtttt aactatgtat atcgaaaaga tatagctaga tctaaatatg aagtaccatt
     1561 ggtatacttt gacttaggta acgatttcca agatttagcc aatgccattt atcgagatga
     1621 tagaaatgtc gatattggtt atgattttat tagaggcgct gatatcaact ttgatattca
     1681 tgaacagctc tattcttatt atttccttct tagatcaata cttaagggta tttttccaat
     1741 acaaaatctt ctgaattacg atgacattct aaatgttact actaagttag atctgcaaga
     1801 aatgtcagac agttatgtta atattttaca agtttaattt ttctaaaatt aacataatac
     1861 gccttatacg aaacgtgtta taatttacct tgaaattcat acacttattt caaagtttat
     1921 agcccgagca tcactgttcg ggcttttttt atgatttttc acgttccacg cctattcttt
     1981 gatcaatgtt ccacaacttt gttcatatca gttaaacaat cgtggttaat catagggtct
     2041 aaaaaaacgg ctccaaaagc ctcttttttc cggttcctgt tcaatgtgct ccggtactgg
     2101 aatccgctta ttttcagtct gccccggcgt agtcaataca tcatgttttg gctcagattc
     2161 tggccgagga ttagttacta tatcttggct aggtttctga tcggtaaacg tagtcatatt
     2221 agtctttggg gcttctagca gtcgttgtat tgcttctatt tgattgtctt ttgcttctag
     2281 ctgttctctt tggaaaactt ctcgctcatg tgcaagttct agctgttttt taagtatgtc
     2341 tacttgctgt tttagcatgt ctacttctgt atctttttgt accgtagtag actgtaacgt
     2401 agttacatta ttatttttat tagatggttc gccaaatact cgtatggctt cagataaatc
     2461 tattttccca tcactatttt ttgataaatc tcctttattt atacgcttat aaatactttg
     2521 tcgattcatc ccataaagtt gtgataactc aataacagat agtgctttca tcatgtaacc
     2581 caagtaaaaa tctgtatgct tgtagacaat aattaatcat gtaacgttac atcagtagtt
     2641 taacgatagc caactttttc aagataagga tgtaattctt gaaacttagc aggatcttga
     2701 agcatttcag caattcttat tgcaaactgt tgatagcttt cagtgccctg agagtatttc
     2761 cccatttcag gtaattctga aagcttgtta gcaaataggt gacgttgagc atctgttatt
     2821 ctggaaaaaa tatctaatgt attgggatct ttcttgcgtt ctattacttt gttcggattt
     2881 cttttttgtt tgaagctaaa agagaaccca gaaatagagc gaccttcttt atgctgttca
     2941 actttaacga tgatatctgt atgttcattt atctgtttga ctgctgattc aagtacacgt
     3001 cgtttaaatg tatctagtct tgggtaatca tctgaagtta agccaagctt cgaacgaaag
     3061 tcagaaactt caaatatagg agtcttaccg acttcacgcc atgctataag caactcatag
     3121 agtcggatgg catattttcc tgttaattgg gtaacttgtt ttaactgata actcgtaaat
     3181 tgtttttcta aacgtgtaat taagggtact acatccattg taaaagtaac ctctaaggtt
     3241 gcagcatcat caatataaga aatattttga acccatcgag aaattacttt ctttgatttt
     3301 cctgtttttt catcaattgt ttgatagcta aactgtctac caaaaaggtt agtaactgct
     3361 gtttttaggg ctttgtaggc ggcatgctta ttaacattaa atctgctcat atagtcactg
     3421 gcatggatcc gtagcctagt gtttgcatca atccctgttc ttgtttctct tgcttgtaag
     3481 atagccagta gaattaaccg ttgttctacg gtatctaaat tataactcgc attaattaaa
     3541 gcgttatctt ttactattaa ttcactggta ggcatttgct acacctttat tatacgtggt
     3601 gttattacac catcattaat cagtggtgta aataggtgac tttttggtgt tgtataggtg
     3661 actttttggt gttgtatagg tgactttttg gtgttgtata ggtgactttt tggtgttgta
     3721 tatcccttgt atacactata aataaaggct tacagcatgt ctaaaagcat ttaaaaactt
     3781 taaaaataat taaaagccca gtgcatgaaa aattgccctt ggttttcgct acgctcaaac
     3841 tctattgaat ctcgctttcg ctcgattcgt ggggcaattt ttggatcatc cagttgtgac
     3901 tttaagaaaa agcaaagcaa ctcggaattc gctacgctca taaagctttt ttactcgctt
     3961 cgctcggtct agaagcattt tcaagcagtt cgcgcccttc gggacgctct aatctgaccg
     4021 taattaatgg ataggaagct tgtaatcaag aaaaaaccag ccgaaatttt gatgtattca
     4081 gttcgctcgt agacactcgc tctttacata cctatgaggt atagaggaag gccttaattt
     4141 taaggaccac caaaatcata atcattattt tttggttttg gttgtgtcga tggtctaata
     4201 gtttcagata ctttgttctc ttgaataacc ggcttcgctt ccatctgtag ttttaagcct
     4261 attaatggct tgaaatcaac ttgtggatct gggtaacaaa gtgagttttg aagctggtta
     4321 tccattctgc tcaggttcca gtgctcatcc tgaagttttg gcttttgtgt tttaataatt
     4381 tcgttaaagt cttgttcgag ttcttttttg tttttggcct ttttggctaa atggttaagg
     4441 taatacatga cttcgagtga gttatagggc tcattcaagc atgaatagaa aggttcatgt
     4501 tttagtacct gctgcttttg ttccatttct cgctcgttgg cattgtgctg ccgaattttc
     4561 tggatctcaa ccaggtattc cacagtttca ttctgttttc cagcacctaa tttgggttcc
     4621 gggagataca aggtgccgtg gatctcattg tatttctcat agttgtcggc tgatacttgg
     4681 ttcttgatgg tgatatcttg gccgttgatc tggtaggtca cagtagggca gtgctcggcc
     4741 aaagcatcat tgaggtgatt ttcccaggct ttgcggatgt gcagtaagaa tgtggaatag
     4801 tctgcgtggc catcctgcag ctggattttt tggcaccgcc tttagccgga tcttgaggat
     4861 tgtagcgttt gaagtattgt tcggctgtgc gttcaatgcc gtcatcagct ttgagggaga
     4921 acatgaggtg gcaatggggc tgttcttcgc cactgagacg cgatttaacg ttgtgtatgg
     4981 cgtatgaatg cggatattgc tgtggagcta tatgtttgtc taaaaacgtc tctatgagcg
     5041 ttttacgctg gtctggcgtg aattcgttgg ggagggcaat ttcatactcc atatagactt
     5101 tggcattcgc tcgctcataa gtatcagcgg cttgccaaaa ctcaatcgga tctttcaccc
     5161 aggacggcat gcatttgcca agttcaacgg attctaaaag ttcatcactt tttttgcgga
     5221 cgttttcgta ttgcatcaat cgggcaatgt agaggtaatg gcccatggca taacctttat
     5281 ttttgctttt aacactgaaa tggtagctgg ccatgtttac attctctgcg gattttcatt
     5341 tttatgtagt gaaacggaat taaaaatgtc ctgattttgg aatgcctcaa tggctggaaa
     5401 aatcaggttg aaggggtcaa gggggaatcg ccccattgcg tactagtttc tctgatcaag
     5461 agaaactgtc aagtgcgaat tatttaaata aaaaataaaa attcttttca agaaatttta
     5521 ttttttattt aaataattct tttcagactg cccgattcaa atatggtaaa accatgtttg
     5581 aatcggtagc agttctgaaa atttggcggt agttgatatg actcaggaaa aagcaaaaaa
     5641 gcgtggtaga ccttctcaac ttttacaaat ggcagagtta catgctttcg ttgagtttct
     5701 attagagaag gatcctcgct cggaacttca aaatcaggtc attgatgcac tacaagctga
     5761 agattttaat tttgaaatgt tgtctgaagc tcagcagatt ttggtgaaag aagcgctaaa
     5821 accatatcgg gaacatttga agcttcagtt gttgtttgat gagctttctc aaaattttca
     5881 gcacacagag tatgaagaga aattcatgga gctgtaccaa ggttataaac aggatgggct
     5941 agatccagtt gagatgaata ttctgaaaat gatgtgtacg cgttatttga attttaaagc
     6001 acaaaagctt gaatatagtg acttagagct gtatttgggt cagattcaga agaagaatgc
     6061 tagtgaaaaa cgtaaagctg aaaataaacg gaagtttgaa ctcggaggtg ctgttttagc
     6121 tgcatttaag aaacttaatc ttgatattag caaagagact cctgagcaaa ttaccaatcg
     6181 aattgtgaat aataaggtat ttcatgatga ggtcaaaaaa tctaaaattt tttccgaggt
     6241 gagtaagtat aaaaaagaat attttgagag gaataaactc tttattgacg tgcttgaagg
     6301 cttgcatacc tggaaaaaag agggaatgac cttaacccaa attgaaattg agaaagtact
     6361 gcgtaaaaat aatgaataag ttgctgttaa gtctatgagc aaaacatcta taacgatctg
     6421 ccctggacgt tgaatgttct gattgagctt tagcttattg ggctgacaca gcattttgaa
     6481 tgctccctgt ttctgcattt ttagtcgcgt tactgcaatg ggaataagca caatcactaa
     6541 aaattttagg attgtgggac agcctgtata aattgctgct gaatatctga gaaatttgca
     6601 tctgggtatt cagctaaata atgttcagtc caaaataaaa cagcttcatg tccatcaaaa
     6661 gaaggattca catctaaata aaaagccaag cgggtactac gttgaatata aagttcctga
     6721 atcaactgta atccaagttt ttttgctttc gctgaaataa ataattgagg ttgggtgaag
     6781 taattcatat aaaatccgat gtagaagaag aggcttgata tgaataatta aacacataaa
     6841 aatatgtgtt gtaaacatat ttttatgtgt tttttagcat tttatgtaaa aaatagccta
     6901 tgttatactt gcactttatc atttgctaaa tttgaacctc atgccttacg aagaattcca
     6961 tagacttatc ggtaaagcag gtctttcaat taaagaattt gctgaactac ttggaatgag
     7021 acctaactcg attacgaatt ataataaagt tggatttgta ccctctcatt tggctgtgat
     7081 tgtatcgctc atatcaacga tgaaggatga aggttttgat ttttatccga tcttagaaaa
     7141 agtacaggcg tatgagaaag cacgtataga aagtcagtct aaatagaaaa gactaagctg
     7201 gattctttgg actattgttg atcagagagt actacaatca aatgggagct tatagccccc
     7261 atttttgatt tcgtataaga aatttcatgt taaatattaa ttttcatatc taaaataaaa
     7321 tatttatgta tatgatttat ataaattata ttttttgtta caaatgtgat gatacctaga
     7381 gtctcctaaa aaccgttaat gtaattacaa tagtacctat ttttaagtaa gtaattgatt
     7441 tatattgaat atatgtgtta acttaaggtt tgagttcatc tgagaaaaga ataatggaaa
     7501 gaaatctaaa tttagcaaat tcaattttcg aaggctttaa tgataaaaac ggattaatga
     7561 tttgtggcta tgaatgggga tggtcaaaag aagatcaaaa actggatgaa agcgatagcc
     7621 gtcccacagt agatatgagc attgaatgta catttagtaa taaatcccta aggtatggtc
     7681 cttcttccaa tacttggcct tatgataaaa atatcaaaaa atggtttaat ttttggggac
     7741 acccattaaa cgatgaaaat ttaggttcag actttgataa atgtattatt caaactaatt
     7801 gggcctatac gagtaatgca gatatagaaa gctataatcg tttcttagaa aaagaagcag
     7861 ttgataattt tattcatcat attgaagttt tgagacccaa agtaatttta tttatgggta
     7921 gtaagcttat taattttctg agaaatatag atgtttggga taggtttact gctattgttg
     7981 gtccagaaat agaaccctta aaaatggtac aaaaaacaga ttttgatggt actcgattta
     8041 aagtttactt tgataatttt gaacaatgcc aagttgtgtg cttaccacat ccatctagta
     8101 gtagaggtct atctgatgaa tatatcaaat tatttgaacc agaattagga acaatcatta
     8161 ctgaatataa gaaacaaaag ggtattctat aaaactaatt atgaaaaaag tccatctcat
     8221 ccaagcagat ataacagctt ttgctgtaca tgccatcgtt aactcagcaa ataaatcttt
     8281 gcttggtggt gggggacttg attatgtaat tcataaaaaa gctggaccat tgatgaaaga
     8341 agaatgtgtt cgtctaaatc aagaaaaagg tggatgccct acaggtcaag cagaagtaac
     8401 aacagcaggt aatctgcctg ctaaatacct tattcatgcg gttggaccac gatggttaga
     8461 tggcgaacac aatgagccac aattgctttg tgatgcttat agtaatgcac tattcaaagc
     8521 taatgaaatt catgcactca ctgtttcttt tccatgtatt agtacaggtg tttatggttt
     8581 tccacctcaa aaggctgctg aaattgcgat tggcactata ctctctatgt tgcctcaata
     8641 tgatcatgtt gcagaagtat tttttatctg ccgagaggat gaaaattatt taatttataa
     8701 gaatctttta tcgaatatcg atgatccaaa tattcagatt ctgatttctt agtttaaatt
     8761 atcgaaagct cgtataagaa ttcagccaca attttggcta ataattcatg ttcttcttgt
     8821 gattcaacaa tatcgaccat caaatcatct aaacctgttt gttcctgaat ggtaacacct
     8881 tgaatttcga gataagtcag cattacagct aaaccagttc ttttattacc atcaacaaaa
     8941 gcatgacctt ttgagatagc tataccaaac catgctgcga tctcaaaaat gtcgcttaca
     9001 ttactgtaat acatctgctg atctatacgg cttaatacgc tttctagctt ttcaatatga
     9061 cagcctattc gccctacgcc agtttgtaat aggatctcat catgcacagc aatgacgaat
     9121 gttgagctaa tcaaactcat ttataagcaa gctcttggat ttcagcttta tgctgattaa
     9181 tgactcgttt tgcactatgc atcacaacac gtttagtcgc ttcgtcattg ctaaattgga
     9241 ttggtaatac ttgacccgaa accttttcac gatttgaaat ttgatcaagg ctatagtctt
     9301 ttaaacgctt tctcatcttc atacccctat taaaagagaa tttcatttta gcctaaatta
     9361 aacaattaag tacgatagtg aggatctttt cttggaatag cattttctga atctatatca
     9421 aagaaatcat aaattttagt ggctgttact gtgttaaata attttttgtc catatacacg
     9481 attgcttttt ctttatttgc gtcgaaaact accctacccc ttggaatctg ttcatattca
     9541 taatgacgta gttctgggta tacactttgc atatttttcc aataatcgac atgtgtatat
     9601 ggactatcaa ttaagcctat tgaatctgca tcatttaaat tgaatggatg agctatgccg
     9661 ataacttgct tttgcaaaaa ccaaaaaatt cctattttca tttttcatca actgattaat
     9721 ttccctaaaa ttaacataat acaccttatg cgaagtcaaa aatgggagct taagctccca
     9781 tttttactga tgatttttta attctttgag catagcatcc cgcaaaattt cattcattcg
     9841 tgtttgatag cctttgcctt gagctttaaa ccatgcaagt acatcagcat ctaaacgaat
     9901 ggaagtctgt tgcttcactg ggcgatagaa ttgattatgg cgtacagcat tgctccagtc
     9961 ggtaatttca ggcatatctg agagatctag ctgatcgtca ggaaccgttc ccttagcaag
    10021 caagcgttga atttcagcat cttgcttgtc gctgaatttt tcattcagtt ctttgcgtga
    10081 gtatctaacc atgctcatat ctatctcgct ccgccttagt tacttgtctt gcactaatga
    10141 ttcgtatgat ttcacagtca tcttcatcaa agatggtgtg agctactaag agcattagta
    10201 cgccttttac ttttccaatg gtttgccaac gttcttcacc atcggtatgt ctgtcctgga
    10261 ttgagatcct cagtggatct tcaaaaacaa ggcttgctgt ttcgaaagag acatcgtgct
    10321 ttttctgatt ctttcgattc tttgcctcat cccattcgaa atactgttcc ataaaaaaca
    10381 ttcaaatttg tatatacaat aatgtatcac aaaattgtat cactcaagct gaaaagcgta
    10441 atttttagtc agatttattg gcaagaacta tcaaaactgc tcataa
//
