LOCUS       CP076399               24013 bp    DNA     circular BCT 21-FEB-2023
DEFINITION  Acinetobacter nosocomialis strain 2S8-227 plasmid p2S8-227-mcr4.3,
            complete sequence.
ACCESSION   CP076399
VERSION     CP076399.1
DBLINK      BioProject: PRJNA734730
            BioSample: SAMN19522711
KEYWORDS    .
SOURCE      Acinetobacter nosocomialis
  ORGANISM  Acinetobacter nosocomialis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 24013)
  AUTHORS   Huang,T.-W. and Lauderdale,T.-L.Y.
  TITLE     Genome analysis of Colistin-resistant Acinetobacter nosocomialis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24013)
  AUTHORS   Huang,T.-W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2021) Department of Microbiology and Immunology,
            School of Medicine, College of Medicine, Taipei Medical University,
            No. 250, Wuxin Street, Taipei 11031, Taiwan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2020
            Assembly Method                   :: Unicycler v. 2020-08
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 440.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/04/2021 17:42:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,260
            CDSs (total)                      :: 4,165
            Genes (coding)                    :: 3,283
            CDSs (with protein)               :: 3,283
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 882
            CDSs (without protein)            :: 882
            Pseudo Genes (ambiguous residues) :: 0 of 882
            Pseudo Genes (frameshifted)       :: 748 of 882
            Pseudo Genes (incomplete)         :: 115 of 882
            Pseudo Genes (internal stop)      :: 49 of 882
            Pseudo Genes (multiple problems)  :: 29 of 882
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..24013
                     /organism="Acinetobacter nosocomialis"
                     /mol_type="genomic DNA"
                     /strain="2S8-227"
                     /host="Homo sapiens"
                     /db_xref="taxon:106654"
                     /plasmid="p2S8-227-mcr4.3"
                     /geo_loc_name="Taiwan"
                     /collection_date="2012"
                     /collected_by="National Health Research Institutes"
     gene            join(22257..24013,1..1330)
                     /locus_tag="KNV99_21115"
     CDS             join(22257..24013,1..1330)
                     /locus_tag="KNV99_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WDM41723.1"
                     /translation="MKNDVRRLSILSTDEIDELFGLPHFSDDDRRLYFDLSAKERELFD
                     NTRTFSVAAHLVLQLGYFKAKRQFFSYEQESSVLNDLDYIAALYFPTKTLSRLKSPSRP
                     IRAEQQRAILDLFQYKQCDNEVKVDLEDKAQRVAMLSTQPIFIFRELTQYLALHRIVMP
                     SYRYMQEMIGRVVAYERTRIARLLSTCMTSLIDQQLAALLRADSGVFRVSALKHEAKDF
                     SYKELRHEVARRQFFQPLHEFAKQFLITAGISNESGKYYASMVKFYTTYKLQRMKKETA
                     QLYLLFFAFHRFQQINDNLIEALLHWVDQYEKQAKRAAEEAMNNAVTNAAKNLQAAGHV
                     LSLFTDDTITDDTPFSIINEKAYALLEQERFPLVADYLRNIAFDKTAFEWLHYTKLSAT
                     FKRNLRQLFTDLDFAGRVEDSPLLEAIGFLQNLLRTEKSPRQTDPNAFPTEIIPKGLRR
                     YLFSKEGKTFKTLDVDRYEFLVYRLLRNSLEAGDVYVKHSNEFRRFEDDLISDLRWQDK
                     ERVLQDIGAPILLAPIQDTLAVFHTMLAARYSAINQHISDGVNKHIKVIGAAEKRRWKL
                     LYPSSDESVNSDFYSQLPGIGIADLLWFVAGNTGFLNAFTHVLDRYVKHEADPREIFAC
                     IVAMGTNMGLAKMAEVSGLSSASMAGTSRNYLRLETLRAANDAISNATSQLPAFHLYDI
                     QDSLHSSSDGQRMETQINTLNARYSPKYFGLQKGVSAYTLVANHVPINAKIIGTHEHES
                     HYVFDLLHNNTSDIKPERHSTDTHGTNQVNFWILHAFGYHFAPRYRDLHKKMDTLVGSQ
                     HPNECGDWLIKPARKTNDELIEREWPNIQRIMASLAQKDVTQATIVRKLSSYSRQNQTK
                     KALWELENICRTLYILDFVDDVGLRQCVQKALNRGEAYHRLRRAVAFVNGGKFRVKTEE
                     EQQIWNECSRLITNAVIYYNTVLLSRVYEQKQTVGDQNALAQLHSISPVAWQHINMYGN
                     FEFSPSTSKIDIDALVARYAEPEYWKQALIENENSSDEF"
                     /name="Tn3 family transposase"
     gene            complement(1372..1635)
                     /locus_tag="KNV99_21120"
     CDS             complement(1372..1635)
                     /locus_tag="KNV99_21120"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41724.1"
                     /translation="MLTTEKVFESLIKLGFKEPSEQPNIKVERLNHDDLTYSVYVKPLQ
                     EKNLKTKYILAVHPHYLLANFESLATDIRNSQLKAWKEKLSG"
                     /name="hypothetical protein"
     gene            2883..3551
                     /locus_tag="KNV99_21125"
     CDS             2883..3551
                     /locus_tag="KNV99_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WDM41725.1"
                     /translation="MIEMTTRIYLRASTKDQDAQRALQVLQDLNQHLNLGETIVYVENY
                     SGTRLDRPELNKLLSEANDGDALLVESVDRLSRLSQADFQELKRRIQEKGLRLIVADLP
                     TTHQLIQTNDSITSSILDLINNMLIDLLATMARLDNEKRIERIKQGLERSGYKPSGKKP
                     NEAKHKRIKELLATNNMTKQEIAKAVGCGVATVYRVMNQGEDAELNQLSNLVHNKAGFE
                     "
                     /name="recombinase family protein"
     gene            3750..4205
                     /locus_tag="KNV99_21130"
     CDS             3750..4205
                     /locus_tag="KNV99_21130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41726.1"
                     /translation="MKISTQIKLTQADILEAIEQYINHHSVNTESMDVEFVGALPDVLT
                     VIVSETEDDNDDQPTTSSMIQPKIADYSQEEDPFTILRSDVDVAEDETVEQRSSSSRPF
                     NPFVDEVKNDSSQVAKEQRKPVSRIDFITGATATQSVKRLPGRGIFD"
                     /name="hypothetical protein"
     gene            complement(5179..6398)
                     /locus_tag="KNV99_21135"
     CDS             complement(join(5179..6122,6122..6398))
                     /locus_tag="KNV99_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612090.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISAba19 family transposase"
                     /protein_id="WDM41727.1"
                     /translation="MTKPNYTPEIRERAVQLLIESEKDYPSTWAAITAIAPKIGCTPET
                     LRVWYLKHLDQLNPAKVQQISDQEKMKQMEREIKELKRANEILRKAAGFFRPGGARPPT
                     QIMVDFIHNNKDRYGVEAICRILPIAPSTYYRTLDLTDNPEHRAKRDLHDEYHAEQIKR
                     IWKESSGRYGVRKVWQKLKREGYVIARCTVARLMQKLGIQGVWRGKNKQTTRNRDDQKR
                     ADDLVKRNFNADHPNQLWVGDFTYIQTHSGWVYTAFVIDVFSRAIVGWKVSTRMNTDMV
                     LDALEQALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAET
                     VNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKKRVHSALGYVSPFEFEAMYYDKI
                     NPLGQVA"
                     /name="IS3-like element ISAba19 family transposase"
     misc_feature    complement(6007..6123)
                     /locus_tag="KNV99_21135"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            6575..7381
                     /locus_tag="KNV99_21140"
     CDS             6575..7381
                     /locus_tag="KNV99_21140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41728.1"
                     /translation="MDYSHLQHIALEVCQQADKFNIPYKVYWYSPDSRKANPKTKIGHY
                     VQPNGKCSFTHGCKCIILFKREHFQQIQVIFNLYRGQVPRIKELENFKCTDDIGFDVYQ
                     RVNELHLFIEPDSISSKKFTIEGFKKKIDYWKKCQLQVLENIQPPMTQRDIDSYMNSLT
                     HVFDQAIAYLQAKNNPNAKLRVRRNSGVRHRMVRVDLADRKSKKEGFSDLVFVFSARRN
                     FSHVQLPKEPSPTRKPRSDSIKAQYYANPLAFKYYDNFGIFEIYDR"
                     /name="hypothetical protein"
     gene            7725..9272
                     /locus_tag="KNV99_21145"
     CDS             7725..9272
                     /locus_tag="KNV99_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /protein_id="WDM41729.1"
                     /translation="MARAPKQTKQEPLEVVLWKAADKLRKNIDAAEYKHVVLGLIFLKY
                     ISDSFEAHYNLLVLGEGEFAGADPEDRDEYTAYNVFFVPENARWSFLLNQAKQPNIGKL
                     VDDAMEAIENDNPQLKGVLPKVYARQNLDPTSLGELIDLIGNIALGDAKSRSADVLGHV
                     FEYFLGEFALAEGKQGGQFYTPKSIVSLLVEMLEPYKGRVFDPCCGSGGMFVQSEKFVE
                     SHQGLVDDISIYGQESNQTTWRLAKMNLAIRGINSEHVKWNSEGSFLNDAHKDLKADYI
                     IANPPFNVSDWSGEQLVGDARWSLGTPPAGNANFAWMQHFLYHMSPKGQAGVVLAKGAL
                     TSKTSGEGEIRKALVSEANVIDCIVNLPAKLFLNTQIPAALWFMRRDRVGSTKYKDRSN
                     EILFIDARNLGHLINRRTKVLSDEDIKLITDTYHNWRNQDGEYEDVAGFCASVPLEKVA
                     ELDYVLTPGRYVGLADDEDEFDFKERFTSLKAEFEAQLAEEAKLNQAIAENLAKVIV"
                     /name="type I restriction-modification system subunit M"
     gene            9269..9790
                     /locus_tag="KNV99_21150"
     CDS             9269..9790
                     /locus_tag="KNV99_21150"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="WDM41730.1"
                     /translation="MKHQTEFGEIPIEWQVKQVIDIKNEAKRAIAMGPFGSNIKADNFV
                     ESGVPVIRGTNMNFAKFVDGEFVFLTEEKADELLGSNCQPNDLVFTHRGTIGQVALIPE
                     NKYPRYVISQSGMKLTVNHEIMDSEFLFYFFKSEYGQYQILKYESQVGVPSISNPLTSL
                     KEMCVPVPPP"
                     /name="restriction endonuclease subunit S"
     gene            9780..9932
                     /locus_tag="KNV99_21155"
                     /pseudo=""
     CDS             9780..9932
                     /locus_tag="KNV99_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034130.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /name="restriction endonuclease subunit S"
     gene            10109..10519
                     /locus_tag="KNV99_21160"
     CDS             10109..10519
                     /locus_tag="KNV99_21160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41731.1"
                     /translation="MSKLNPRVPRIWLVSNVEDNYVCSTEFQNLKPKVTAHLMFLYSLF
                     NSRDVIDELTMSASGTSGSHQRVKPEDMLNITFRTPCLDYLEQYSSLIAPSMQKVASNK
                     QQIQTLENLRDTLLPKLMSGEVRVQYQTEEVA"
                     /name="hypothetical protein"
     gene            10573..11110
                     /locus_tag="KNV99_21165"
                     /pseudo=""
     CDS             10573..11110
                     /locus_tag="KNV99_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871208.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /name="DUF2939 domain-containing protein"
     gene            11128..11655
                     /locus_tag="KNV99_21170"
     CDS             11128..11655
                     /locus_tag="KNV99_21170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41732.1"
                     /translation="MFDNQNMQNDGNKTIKLILAIGLVVAALVASAGVFYYYVVFVPNL
                     EQQKLDLEKQKLKEKQDAEAKALQKKEQETEQRAQAYQNCVDNAEIAYTSNWSSACQKL
                     AERNQAALDNCLNNPSIINNQFMGKSYCEKVYGATEFDPECTLPGNTAEGIEKARSERK
                     DACMVEAQQALY"
                     /name="hypothetical protein"
     gene            11703..14944
                     /locus_tag="KNV99_21175"
                     /pseudo=""
     CDS             11703..14944
                     /locus_tag="KNV99_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /name="type I restriction endonuclease subunit R"
     gene            complement(15015..15929)
                     /gene="repM"
                     /locus_tag="KNV99_21180"
     CDS             complement(15015..15929)
                     /gene="repM"
                     /locus_tag="KNV99_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WDM41714.1"
                     /translation="MKNDLIVKDNALINASYNLDTTEQRLILLAIVQAREVSKDVDANS
                     TLEVHAHHYMKQFNVDKHAAYEGLKNAASNLFERKFSYKSIHEGTQQEKIVKSRWVSKI
                     AYVDSAGIVELTFAPDVIPLITQLEKSFTAYELKQISSLTSKYAIRLYELLIQWRSVGK
                     TPMFDIDDFRFKLGLAEGEYAKMANFKVRVLDIALNQINELTDITASYEQHKVGRTISG
                     FSFTFTAKHQPKEVTHTKPKKLSDKQIQFFANKLAHHDPFASQKAARGESYVDLEKRLL
                     IELQDAEVVRKYADVLKELGLDV"
                     /name="repM"
     gene            16378..17007
                     /locus_tag="KNV99_21185"
     CDS             16378..17007
                     /locus_tag="KNV99_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WDM41715.1"
                     /translation="MILLIGSQKGGCGKSTIAINIAAYLAKHNKDIVLVDADPQQSSAN
                     WVKDRDDTNLPKVHCVQRYGDIKNTLKDLDSRYDYVIVDVAGHDSKELRTAMLTAHYLV
                     VPFRPSQFDLDTLPHLSEVIDQAKSFNEGLKPFGLLTLAPTNPSVSEVQQANDYLADFP
                     LFTALSSVIHDRKVYRDVTAEGKGVVESNNPKAIEEFESFMKELLA"
                     /name="AAA family ATPase"
     gene            17004..17240
                     /locus_tag="KNV99_21190"
     CDS             17004..17240
                     /locus_tag="KNV99_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41716.1"
                     /translation="MIKKREIAKPSSPDMEAFINGANEETTEKLDPKAPRKFKTHTIPM
                     NEYEYQLLTELAEKYGQTHNGIIRFALKKLSEE"
                     /name="hypothetical protein"
     gene            complement(17345..17635)
                     /locus_tag="KNV99_21195"
     CDS             complement(17345..17635)
                     /locus_tag="KNV99_21195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41717.1"
                     /translation="MQNLVEFSQIDSDISEEEIIERAVIAAIELYAKRLLPTSKRTKST
                     VYLSPELDSHLNSIKEFLGISRQDLVEEAVMFYTRWKIYARHINRSILDKI"
                     /name="hypothetical protein"
     gene            complement(17854..18057)
                     /locus_tag="KNV99_21200"
     CDS             complement(17854..18057)
                     /locus_tag="KNV99_21200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDM41718.1"
                     /translation="MANQTPYQTTLIEITETNIIGTKHLTVKMDEHRYKQLRLNALDKD
                     MTHRDIMLEAFDLWMKVHGTTF"
                     /name="hypothetical protein"
     gene            complement(18254..18904)
                     /locus_tag="KNV99_21205"
     CDS             complement(18254..18904)
                     /locus_tag="KNV99_21205"
                     /inference="COORDINATES: protein motif:HMM:NF024783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="WDM41719.1"
                     /translation="MGKISLSAHGIYLNENIESKTSFAGFPPNPKNSGQAITFNFTDHS
                     AVQAFCKLVLKINASTSNEIIPYLRQDSDVSDKAVISEEEALLPSTELNALVKVRLGQS
                     NFRRELLKYWQGCSVSDCTFEKLLIASHIIPWSEQEDSRLNPYNGLLLSPNLDAVFDKG
                     YISFNDDGSIMISSELSPADLSALNLSSSLKLRKLDAQHLPFLAWHRENLFKA"
                     /name="HNH endonuclease"
     gene            complement(19106..20731)
                     /locus_tag="KNV99_21210"
     CDS             complement(19106..20731)
                     /locus_tag="KNV99_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006081694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-4.3"
                     /protein_id="WDM41720.1"
                     /translation="MISRFKTLSVNQFTFITALFYVAIFNLPLFGIVRKGIEKQPEVDP
                     LFIASMPLFLTFALSFLFSIFTVKYLLKPFFIVLTLLSSSVFFAAYQYNVVFDYGMIEN
                     TFQTHPAEALMYVNLASITNLLLTGLLPSYLIYKADIHYQPFFKELLHKLAFMLLMFVG
                     IGIVAFFYYQDYAAFGRNNSELRRYIVPTYFVSSASKYLNEHYLQTPMEYQQLGLDAKN
                     ASRNPNTKPNLLVFVVGETARSMSYQYYGYNKPTNAHTQNQGLIAFNDTSSCGTATAVS
                     LPCMFSRMGRADYDPRRANAQDTVIDVLSHSGIKVQWFDNDSGCKGVCDQVENLTIDLK
                     SDPKLCSGQYCFDQVLLNKLDKILAVAPSQDTVIFLHIIGSHGPTYYLRYPPEHRKFIP
                     DCPRSDIQNCSQEELINTYDNTILYTDFILSEVVNKLKGKQDMFDTAMLYLSDHGESLG
                     EKGMYLHGAPYSIAPKEQTSVPMLAWVSNDFSQDNQLNMTCVAQRAEQGGFSHDNLFDS
                     LLGLMNVKTTVYQSQLDIFAPCRY"
                     /name="phosphoethanolamine--lipid A transferase MCR-4.3"
     gene            complement(20904..21493)
                     /locus_tag="KNV99_21215"
                     /pseudo=""
     CDS             complement(20904..21493)
                     /locus_tag="KNV99_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            21663..21923
                     /locus_tag="KNV99_21220"
     CDS             21663..21923
                     /locus_tag="KNV99_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WDM41721.1"
                     /translation="MNTLSANEAKIHFGDLLLKAQQAPIQINKNGKPVAVVISADAYQS
                     IETLKLHLLQSKAVKAMTDIKMGNLVDGNTFFDELAAGQYD"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            21923..22234
                     /locus_tag="KNV99_21225"
     CDS             21923..22234
                     /locus_tag="KNV99_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WDM41722.1"
                     /translation="MPVTYHLTPDAQSDLIGIHRFTLAQWGTTQSKTYLSGLRQTIQLL
                     AETPTLGKNRPEVRVNVFSFPYSSHVIYYIQHEHQFVVFGILHKSMVPLAHLAEREII"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     ARG             complement(19110..20731)
                     /name="mcr-4.3"
                     /gene="mcr-4.3"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-4.3"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(5183..6081)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.667"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagcgatttt tacagccagt taccaggaat tggtattgcg gatctactgt ggtttgttgc
       61 gggtaatact ggatttttaa atgcatttac ccatgtatta gatcgttatg taaagcatga
      121 agctgatccc cgtgaaattt tcgcctgtat tgttgcgatg ggaacaaaca tggggttagc
      181 aaaaatggct gaggtttcag gccttagctc agcatcaatg gcggggacgt ctagaaatta
      241 tctacgcttg gagacgttgc gagcggctaa tgatgcgatt agtaatgcaa ccagccagct
      301 acccgcattt catctctatg atattcagga ctcgctccat tcaagtagcg atggccagcg
      361 gatggaaacg caaattaaca cccttaacgc tcgatattct cctaagtatt ttggtttaca
      421 gaaaggtgtc agcgcctaca cgttggtcgc aaatcatgtt cctataaatg caaagattat
      481 tgggactcac gaacatgaaa gccattacgt tttcgattta ttgcataaca atacctctga
      541 tataaagcca gaacggcatt ccactgacac ccatggcacc aatcaggtta atttttggat
      601 tttgcatgca tttggatatc attttgcacc tcgttatcgt gatttgcata agaaaatgga
      661 tacgttagtc ggctcacaac atcccaatga gtgtggtgac tggctaataa agcctgcccg
      721 aaaaaccaac gatgaattga ttgaacgtga atggcccaat attcaacgta tcatggcatc
      781 gctagctcaa aaagatgtta ctcaagccac gattgttcgc aaactctcga gctattcgcg
      841 tcagaatcag accaagaaag cattatggga gttggaaaac atatgccgaa cgctatacat
      901 tctcgacttt gtcgacgatg ttggcttacg tcaatgtgta caaaaagcat taaatcgtgg
      961 tgaagcctat catcgtctca ggcgagcagt tgcatttgtt aacggtggaa aattcagagt
     1021 aaaaacggaa gaggagcaac aaatttggaa tgaatgctct cggctcatca ccaatgcggt
     1081 catttactac aacacagtgc tgctatctcg tgtctatgaa cagaagcaaa cagtaggaga
     1141 tcaaaacgca ctggctcagc ttcacagtat ttcgcccgtt gcatggcagc acatcaatat
     1201 gtatggcaat tttgagttta gtccatcaac ctcaaaaata gatatcgatg cattggttgc
     1261 tcgatatgct gaaccagaat attggaagca agcgctgatt gagaacgaaa attcaagtga
     1321 tgaattttag agcttaaagc taactttaca tttttggcgc gttgggcaaa tttagccaga
     1381 aagcttttcc ttccatgctt taagctgcga gtttctaatg tctgtagcta ggctttcaaa
     1441 gttagctagc aaataatgag gatgaacagc caaaatatac tttgttttaa ggttcttttc
     1501 ttgtaatggt tttacatata cagaataagt taaatcatca tgattaagcc gttctacttt
     1561 aatgttgggt tgttcacttg gttctttaaa gcccaatttg attaatgatt caaatacttt
     1621 ttctgtggtc aacatagttg agtttcccaa ttttagtttc tctttactta tgggacacat
     1681 tccgccctca tcaattactt tgacttgaaa aggtgaaaaa caaggttcat tgactctaca
     1741 tgtgtcaggt agctaaggcc cgattatgat aaatgtggtt aggaattcaa taggttacat
     1801 aggctaaatt gaaaatttcc aaacttctct cagttatggc agtagtccaa attaactgac
     1861 gaaactctgg tttgaatgat aagaatgaag cttctagaag gcgaaacccg tagagataac
     1921 tgtctcaaat tgagatagtt tgaaatcatc tatctcaaat tgagacactt agaccagtac
     1981 agagtaccta tttgcctcaa ttggtatcac agagatacca attggtaatt ttgagtttgg
     2041 cacctccggt gccttttgtg agtcgcgcaa tgataagaag aatagtgacg attcctttgt
     2101 aaaaccaaag tgctctacca atcagcctag aaagctaatg acatggccag tttgccctat
     2161 gggagtaagg caagcgacta ggtttgattg gtagaggatt tatcgtagag catgattttg
     2221 gtgtggttaa ctaacttatg cctcagatta ctgttgatct gatattgctc tattggataa
     2281 aacaatacga ttgtacatga atgaacgcgc aggattaccc atccaattgg atttcatgca
     2341 agcagagaga tcggataggt gtctgtgtga taccaattac ccctgaactg tctcaatttg
     2401 agatagttgg acgtcaatta gtatcactgt gagactgatt gaacacactg cgaagctcag
     2461 cacttaccat gcctatgagc caacacccgt caaggtgatc ggtgaagtag gcaatggtaa
     2521 gtggctaaac gactaatcgc attggaatag taactccccg tttgaatgaa ttgccttacc
     2581 atcctcaaat ctaccaatta tctgtgctgt tgttcgatta tcagttgtga agattagatc
     2641 ctcatcaatt cgaaatggta gcatctcacc agtcgatata ctgtagacaa actccccgtc
     2701 aaaatctcca attaagttca tatctttgtc ataagcatcc attatcaatc gttcctttga
     2761 gttatgtatc aatagactat acctgaatga taaagaccca aacagcattt aatgtctatc
     2821 aatagactta attttgcaat taaatgatag tattatcatt gtactattta agccaagtac
     2881 aaatgataga aatgacaaca cgtatctacc tcagagcatc aaccaaagat caagacgcgc
     2941 aaagagcact acaggttctg caggatctaa accagcacct taacttgggt gaaaccattg
     3001 tgtatgtaga aaactacagt ggtacgaggt tagaccgtcc tgaattgaat aagctactgt
     3061 ctgaggcaaa tgatggagat gcactattag ttgaaagtgt ggatcgtctg tcacgtctaa
     3121 gccaggcaga cttccaagaa ctcaaacgaa gaatccaaga gaaaggtttg cgactcatag
     3181 tggcagatct accgactacc catcaactaa tccaaaccaa tgacagcatc actagctcga
     3241 tcctagatct catcaacaac atgctgattg atctacttgc tactatggct cgtttggata
     3301 acgagaaacg tattgaacga atcaaacaag gcttagagcg ttcagggtac aaaccatcag
     3361 gtaagaaacc aaatgaagcc aagcacaaaa gaatcaagga gttgcttgct acaaataaca
     3421 tgactaagca ggaaattgcg aaagctgttg gttgtggggt agctactgtg tatcgagtaa
     3481 tgaaccaagg agaagatgca gagctgaatc agctttcgaa tctcgtgcac aacaaggcag
     3541 gattcgagta gccattgaag acctttagtt gaaatgttag ccactttttt ggtttctatc
     3601 gtcactgccc tatttcacca ctccgctgtc gtttgtggta catacccata aatgtttaaa
     3661 accaagagtt aagtgtaagt gccgcagtcc ctgcggtggg agtgagcttt acagctcaat
     3721 caataactct cacttaagga aaacaccaca tgaaaatttc aacccaaatc aaattaaccc
     3781 aagcagatat tctagaagca attgaacaat acattaatca ccatagtgta aatacagaga
     3841 gtatggatgt agagtttgta ggagctttac ctgatgtgct tacagtgata gttagtgaaa
     3901 ctgaagacga taatgatgat cagcccacca cctcatctat gatccaacca aaaattgctg
     3961 actactcaca agaggaggat ccattcacta tactacgttc ggatgttgat gttgctgaag
     4021 atgagactgt tgaacagcga tccagttcgt caagaccgtt taatccattt gttgatgaag
     4081 tgaagaatga ttcaagccaa gttgcaaaag agcagcgaaa accagtttca agaattgact
     4141 tcatcacagg tgctacagcc actcaatcag tcaaacgatt gccagggagg gggatatttg
     4201 attgactcag gattagtgtc aaaagttttg agaaaattgc tcacattagt acgacgatag
     4261 ggttcttaca tttcgtacac ccatgggggg attcaccccc ggggtacaac tcagaaaaag
     4321 gattcttacc caacacaaaa cacatcagta ttttgagaca gttttcaaga taggataaat
     4381 tgtctgatct agttctgatg ctattacttc aattcatgaa atgctcaact cactgttgta
     4441 cttcaatgtg gtgatcttca cagatgaagc catgagtgtt aggtgcagtt gatctagcca
     4501 ctggatagga taaaccaaga taggataaac caagatagga taaaacaaga taggataaaa
     4561 caagatagga taaaacaaga taggataaaa caagatagga gtaatcctcc gatgagcccc
     4621 actttttcta attcattaat accgcgatag tgtccactac attttaagtg ggcacctatt
     4681 tatggattta acgacggtta tagagacagc acgccagtga tgtgctgaga aggttgccga
     4741 cacataaagt gaccaagatt gaggaagtac tgccacatcg ctgcatatcc aacccaagtt
     4801 aaaaaatagc gatggggttt agcggatgct tcaagatagg ttactctaag ataggataaa
     4861 acatgccgca aagtccctag atctatgtcg taacctctca cagagttctt ctatgttgag
     4921 tctatgtggc ataggggtga aaagtcataa gtttagtagt ggtaagttcc tagctatctg
     4981 gtctgtacta tgtcgggtgg atagccattg agtgaaaaac tactatgtta tcactatgat
     5041 ttgatcggat ttttgattgg ttataaagta gctaaacgat tgattgttat gttgaacata
     5101 tgaatagttt gtatataata taagtatact attaattaat gaaccgtacc gggtttgtcg
     5161 gagacttttt tatttaagtt aagccacctg acctaacggg ttaatcttat catagtacat
     5221 tgcttcaaac tcaaaaggcg atacataacc cagtgcactg tgtacacgct ttttattgaa
     5281 ccaatctacc cagtttagtg tcgcaagttg tacatctgct aaaccttgcc aatctgcttt
     5341 taaatattca atcacctctg ttttgtataa gccattcacc gtttcagcca gagcattatc
     5401 gtatgaatca cccgttgtac cgactgatgc tcgtaaattt gctgcttcta aacgattggt
     5461 atagcgaatg gaaagatatt gcacacctct gtcggaatga tgaatcacat tctttggcat
     5521 gcctcgatca tgcaatgctt gctctaatgc atcaagcacc atatctgtat tcatccgtgt
     5581 agatactttc catccaacaa ttgctcgtga gaacacatca ataacaaatg cggtatatac
     5641 ccagcctgaa tgagtttgaa tatacgtaaa gtcacccacc catagttggt ttggatgatc
     5701 agcattaaaa ttacgtttca ctaaatcatc tgcccgtttt tggtcatctc ggttacgggt
     5761 ggtttgttta ttcttaccac gccaaacacc ttgtatacct agcttttgca tcaatctagc
     5821 aactgtacaa cgtgcaataa cataaccctc acgtttcaat ttttgccaaa ctttacgtac
     5881 accatatcga cctgaacttt ctttccaaat tcgtttaatt tgttcagcat gatactcatc
     5941 atgtagatct cgtttcgctc gatgttctgg attgtcagtg agatctaaag ttcggtaata
     6001 ggttgaaggt gcaatcggta aaattctaca aatcgcttca acaccatatc gatctttatt
     6061 gttatggata aaatccacca ttatttgtgt gggcggtcga gctccgcctg ggcgaaaaaa
     6121 gcggctgctt tgcgtagaat ttcattggca cgttttaatt ctttaatttc acgttccatt
     6181 tgcttcattt tttcttggtc agatatctgt tgtactttgg caggatttag ttgatccaga
     6241 tgctttaaat accaaacacg caatgtttca ggagtacaac cgattttagg agcaatagct
     6301 gtgattgctg cccaagtaga aggataatct ttttcagatt caattagtaa ttgaaccgct
     6361 ctttctctaa tttcgggggt atagtttggt tttgtcatcg ggatagtctc tcagaatatt
     6421 gactctccga caaacccggt acggttcatt aatggtaggg gtggctatat tctaaatata
     6481 ggtatttaga acaactctta taaaaatgat agatatagta taagaagaac aattctctta
     6541 aaatccatga aatttgagta ttctggtttc atttatggat tattcacatc ttcaacatat
     6601 tgctctagaa gtttgtcagc aagcagacaa attcaatatt ccttataaag tgtattggta
     6661 ttcacccgat tcaagaaaag ctaaccctaa gacaaagata ggtcactatg tacaaccaaa
     6721 cggaaagtgt tcgtttactc atggttgtaa atgcatcatt ttgtttaaaa gggaacactt
     6781 tcagcagatt caagtcattt tcaacctata tcgaggacag gttcctagaa tcaaagagct
     6841 tgagaatttc aaatgtacag atgatatagg ctttgatgtc tatcaaagag taaatgagct
     6901 tcatctgttt attgagcctg acagtatttc ttctaaaaaa tttacaatcg aagggtttaa
     6961 gaagaagata gattattgga agaaatgtca gcttcaagtg ctggagaaca ttcagcctcc
     7021 tatgacccaa agagacattg atagctatat gaatagtctc acgcatgttt ttgatcaggc
     7081 aatcgcttac ttacaagcca agaataatcc caatgcaaaa ctcagagtaa gacgaaatag
     7141 tggcgttaga cacagaatgg ttagagttga tctagctgat agaaagtcaa aaaaagaggg
     7201 attctctgat ttggtttttg tatttagtgc aagacgtaac ttctctcatg tccaattacc
     7261 taaagaacca agcccgacaa gaaaaccaag atcagacagt attaaggctc agtattatgc
     7321 aaatccattg gctttcaaat attacgataa ttttggcatc tttgagattt atgatcgcta
     7381 ggtaactagc atatcaatgt gcttcgtatc tataactcgt cgcatcttcg cacgctcgat
     7441 gctcctcgtt gatacgaagc acgcttcgca cctcttttct tggtttattg gtctttcatt
     7501 cgctggcctt cacttaagca tatactctcg tcacaaatac tgcgtatttg ctcctcgcta
     7561 tatgcttaag tgaagggagc agagcatcct caatcttcat tcattggttt caaggaataa
     7621 agagcacgtc aaatcatgtc acttacgtac ttacacacgc acgcataatt ggttactatt
     7681 caattaacaa atattagata agctattcat tgcaggttga ttttatggct cgtgctccaa
     7741 agcaaaccaa acaagaacca ttagaggttg tactgtggaa agcagcagac aaacttcgca
     7801 agaacattga tgctgctgag tacaagcatg ttgttcttgg tttgatcttc ttgaagtaca
     7861 tctctgactc atttgaagcc cactacaact tactggtttt aggtgaagga gagtttgcag
     7921 gagctgaccc tgaagaccgt gatgaataca ctgcatacaa cgtcttcttc gttcctgaga
     7981 atgcacgttg gtcattcctg ttaaaccaag ccaaacaacc taacattggc aagttagtcg
     8041 atgatgcaat ggaagctatt gagaatgaca accctcaatt gaagggtgta cttcctaagg
     8101 tctatgcacg tcagaatctc gatcctacga gcttaggaga gctgattgac ctgattggta
     8161 acattgcact aggcgatgcg aaatcgcgct ctgccgacgt tctaggccat gtattcgagt
     8221 acttccttgg tgagtttgct ttggctgaag gtaagcaagg cgggcagttc tacacgccta
     8281 aatccattgt gagcttattg gttgaaatgc ttgagccata caaaggacgt gtattcgatc
     8341 cttgctgtgg ttcgggcggt atgtttgtac agtcagagaa gttcgtggaa tcccatcagg
     8401 gcttggtaga tgacatctct atctatggtc aagagagtaa ccaaaccaca tggcgacttg
     8461 ctaagatgaa cttagccatt cgtggcatta actcagagca tgtgaaatgg aactctgagg
     8521 gttcattcct caatgatgca cacaaagacc tcaaagcgga ttatatcatt gcgaatccac
     8581 cattcaacgt ctctgattgg agtggtgaac agttggtagg tgatgctcgt tggtcattgg
     8641 gaacaccacc tgcaggtaat gctaactttg catggatgca acacttcttg tatcacatga
     8701 gtcctaaagg tcaggcaggc gttgtcttag ctaaaggtgc gcttacttct aagaccagtg
     8761 gtgagggtga gattcgtaaa gccttggttt ctgaagccaa tgtcattgac tgtattgtga
     8821 acctacctgc caagctgttc ttgaatactc agattcctgc ggctttatgg tttatgcgtc
     8881 gagaccgtgt tggttcaacc aagtacaaag accgtagcaa tgagatcctg ttcatagatg
     8941 ctcgtaacct agggcatctg attaaccgca gaaccaaagt gttgtctgat gaagacatca
     9001 agctgattac tgacacttac cacaattgga gaaaccaaga tggtgagtat gaagacgttg
     9061 ctggcttctg tgcttctgta ccgcttgaaa aagtggctga gcttgattat gtactcaccc
     9121 caggtcgtta tgttggttta gcggacgatg aagatgagtt cgactttaag gaacgcttta
     9181 caagcctgaa ggctgagttt gaagcacagc ttgcagagga agcgaagctc aatcaagcca
     9241 ttgctgagaa cttggcaaag gtgattgtat gaagcatcag accgaatttg gtgaaattcc
     9301 tattgaatgg caagtgaagc aagtcattga tatcaaaaat gaagcaaaac gagcaatagc
     9361 catgggaccg tttggttcca atattaaagc agataacttt gtagaaagtg gtgtccccgt
     9421 tattcgaggc actaatatga actttgctaa atttgtagat ggtgagtttg tctttcttac
     9481 agaggaaaaa gcagacgagc tattggggag taactgccaa cctaatgacc tagtattcac
     9541 acatcgtggg actataggac aggtagctct aattcctgag aacaaatacc ctcgttatgt
     9601 aatttcacaa tcaggtatga agcttacagt taaccatgaa atcatggata gtgagtttct
     9661 gttttacttc tttaagtctg aatatggaca gtaccaaatt ctcaagtatg aatcccaagt
     9721 tggtgtccct tcgataagta acccattaac ttcactgaag gaaatgtgtg taccagttcc
     9781 ccctccttga acaaaaagcc attgcctcag tcctatcatc attggatgac aagattgact
     9841 tactgcatcg tcagaacaag accttggaag ccatggctga aacactgttt cgtcaatggt
     9901 ttattgagga agcgaaagag gattgggagg agctttcagt aagtgatatt gctattcatg
     9961 ttaaagacaa tgtgaatcca tcaagggatc ctcttgagac cttttatcat tttagtttgc
    10021 ctgcttttga ctcggacaaa aacctactag tgaattaggc tcggagattc ttagtaacaa
    10081 atatcgagtt tctaagaact caattcttgt gtcaaagctg aacccaagag ttcctcgtat
    10141 atggctcgta tctaatgttg aagataacta tgtgtgttca actgagtttc agaatttgaa
    10201 acccaaagta acagctcatt taatgttctt atatagcttg tttaactcta gagacgtaat
    10261 tgatgaatta actatgagtg cttcaggtac gagtgggagt catcagcgag tgaagccaga
    10321 agatatgttg aatatcacat ttaggacacc atgtttagac tatcttgagc aatactcatc
    10381 attgattgcc ccttctatgc aaaaggtggc gagcaataag caacagatcc aaacactaga
    10441 aaacctaaga gacacgcttc taccaaagct gatgagtggt gaagtgagag tccaatacca
    10501 aacagaagaa gttgcataag attgaggtga agtcatttga tggcttcacc ttttttacat
    10561 caaggggtaa acatgaataa gaagttgata ggggttttaa tagcaattgt agtcgtgatc
    10621 tgtggttacc tgtatgcaac gccatacctt gtactcaata acatcaagaa agcagcacag
    10681 gcaggtgata gcgacacagt atcgaagtac atcgactatc caagtgttcg ccaaagcttc
    10741 aaagaccaaa tgaatgccat gatggctaaa gagctaatga atcaagacac tgatggtttt
    10801 gctgcattag gagcaatgct tgcttcaacc atggtcgata agatgattga tggctttgtc
    10861 acacctgaag ggatgactct aatgttacaa ggcaaagacc taaagcaatc agcacagcag
    10921 tcaatgggta gtcaagattc atcaaaccaa gaaaatgaag aacctaagcc agaatatgaa
    10981 gcaggctata cctcaatgaa tgactttgag gtggtcatta aagatcaaga catccaaaga
    11041 agtcaaagta ctcatggttc gtgatggctt aatttggaag attcacaaga tcgctgtgcc
    11101 aatggaatag taagagttag taagtaaatg tttgataatc aaaatatgca aaatgatgga
    11161 aacaagacaa ttaagttgat cttggcaatt ggtttggtag tggctgcatt ggtcgctagt
    11221 gcaggggttt tttactacta tgtagtcttt gttccaaatc ttgaacaaca gaagcttgat
    11281 cttgaaaaac aaaagctgaa agagaagcaa gatgctgaag ctaaagctct tcagaaaaag
    11341 gagcaagaaa cagagcagcg agcgcaagca tatcaaaatt gtgttgataa cgcagagata
    11401 gcatacacaa gcaactggag tagtgcatgt caaaagctcg ctgaacgtaa tcaggcagct
    11461 ttagataatt gtttgaataa tccaagcatt atcaataatc aatttatggg taaatcatat
    11521 tgtgagaagg tttatggtgc gacagagttt gatcctgaat gcacgctacc aggaaatact
    11581 gcagaaggta ttgaaaaagc aagatcagag cgtaaagacg cttgtatggt tgaagcacaa
    11641 caagctttgt attaagtaag tatgacataa gaatcaaacg agaaaatagg ttggggtaga
    11701 tagtgactaa gatgaccgaa tcagacattg agctgatggc gatagagcaa ctggagggaa
    11761 tgggctatac ctatgtgtat ggacctgaaa ttgaacccaa aggaagtaac cctctgcgaa
    11821 gctataagca ggtcatcttg gaagccaaag tgctagaagc cttgcagcgt ttgaatccac
    11881 atctcagtga agacaaatgt attgaagcac tgaagcaagt caccaatctg tattccccgg
    11941 atatgctcag caacaaccag gcattccatc gcttactcac tgaaggcatc aacattgaag
    12001 tgagcaagga tggtaatact caaggtgaat atgcttggct tgttgacttc aataacccaa
    12061 ccaataacga atttctggtc atcaaccaag tcaccatcaa agaagaccgt tggaccagac
    12121 gaccagatct gatcctgtat gtgaatggct tgcctttggt agtcattgag ctgaagaatg
    12181 ccactgatga gaatgctact gtagatggcg cttacaggca gattcagacc taccaaagcc
    12241 aaatccctag tctctttacc tacaacgcct ttaacgttat ttctgatggt ttagaggcga
    12301 aagcagggac ggtctcagca gacctcagtc ggtacatggc atggaagacg actaatgggc
    12361 aaaccaaagc caagagtact caggcacagc ttgaagtctt actgcatggt ttgctaaatc
    12421 cagtcacatt actggacatg atcaggcact tcattgtgtt tgaatccaat aaacaagaag
    12481 atgctaatgg cttgatcacc atcaagacca tcaagaagat ggctgctacc accagtacta
    12541 tgcggtgaat gcagcagtac tctctaccat tcgtgcatct gcaatgtctt cggactcgaa
    12601 atcagcagaa cttgctatgc aacaccaagg cagaagtaag ctcgaacttg tgcaacaaca
    12661 agcagtaggt gacaagaaag caggtgtagt atggcatacc caaggttcag gtaaatccct
    12721 atctatggtg ttctatacag gcaagattgt cttggctttg gataacccaa cagtcgtagt
    12781 catcacagat cgtaacgact tagatgacca actgtttgga acctttgctg catccagcca
    12841 attactgcgc cagacaccaa agcaagcaga gaatcgagaa gaactcaaag agctactcaa
    12901 ggttggttca ggtggggtga tcttcaccac catccagaag ttccaacctg aagatggtgg
    12961 cagtgtctat gagcagctct cagaccgaac caatattgtc gtaatagcag acgaagcaca
    13021 tcgttcacaa tatggcttca atgcaaaaga ggttgatgtt aaagatgatg atggcaaggt
    13081 tgtcggtaag cggactgtct atggttttgc caagtacatg cgtgatgctt taccgaatgc
    13141 cacttatctt ggcttcacag ggactccgat tgagaagaca gatgtcaaca cgcctgcagt
    13201 ctttggtaac tatgtcgaca tctatgacat ctctcaggct gttgaagatg gtgcaaccgt
    13261 tcggatcttc tatgaaagcc gactcgccaa gattgccatc agtgaggaag gcaaagaact
    13321 gattgaagac tttgatgacc agttcaccga agacgatcta aaccagacac aaaaagagcg
    13381 agcaaagtgg gcaagagtcg aaggactcat tggtagtaaa gagcgtatta aagcgattgc
    13441 caaagacatc gtagaacact ttgaacaacg tcttaaggcc aatgcaaacc aaggtaaagg
    13501 catgattgtc agcatgtccc gcaggattgc tgtcgacctc tacaacgaaa ttgtagcaat
    13561 caaaccagaa tggcacagcg atgacttgca tgatggtgta atcaaggtgg tgatgacctc
    13621 atctgcttcg gatggtgaag aaattgccaa acaccatacg accaagcagc agcgtcaaat
    13681 tctggctaat cgtatgaagg acaatgatga caagctgaag ctcgtgattg ttcgtgatat
    13741 gtggctgact ggtttcgatg caccaagcat gcacacgctc tacatcgaca agccaatgaa
    13801 aggtcataac ctcatgcaag cgattgcacg agtaaaccgc gtctataaag acaagctcgg
    13861 tggcttggtg gtggattacc tcggtattgc atctgatctt aaagaagctc tgtcattcta
    13921 ctctgaagca ggtggtaagg gtgatcctgc actggttcag gacgaagctg cgactatcct
    13981 gcaagagaag ctcgaagtcc ttgatggcat tatgcatggc ttcaactatc aagaatactt
    14041 cacagcagat acttctcgta agctaaacat cattctggaa gctgaggaac acatcctcgg
    14101 tgtagatcag ggcgaaggta agacccgatt cctcaatgcg gtatccgcta tgtcgcaggc
    14161 atttgcatta gctgtacctc atccaagtgc gatggaagca gcaccagtgg ttgcattctt
    14221 ccaggctgta aaagcacgac tggccaagtt tagtgatggt tcaggaaaaa tctcaggcct
    14281 gagtaatgct gaactggaag ccaaggtcaa acagaccatt gaccaggcgc ttgtaactga
    14341 acaggtggtg gacatctttg atgcagcagg aatccagaaa ccagacatct ccattctctc
    14401 tgatgagttc atgcaggaga tgaaaggata caagcacaag aacattgcac tggaaaccct
    14461 gaagaaattg ctcagtgatg aaatcaaggt aagagaacag cgcagcgtta cccagggtaa
    14521 gaagctgatg gatatgctga gctctgccat taagggctat cagaacaagg tgctgactgc
    14581 agccgaagta attgatgaac tcatcaagtt ggctaaggca atccaagagt cagacaaaat
    14641 ggcagaggaa ctcaacctga ctgaatatga atatgcgttc tactctgctg ttgctgataa
    14701 cgagagtgcc agagagttga tgcagaagga cattctcaga gagcttgcag tagtcctcac
    14761 tgagtccatt cggaagaatg tcactctgga ttggaccatc aaagaagctg ctagagcgaa
    14821 gctgagagtc attgtgaagc gtctactcaa gaagtatggc tatccacctg atatggcttt
    14881 actcgcaaca gagactgtct tggaacaggc agagcagttg gcagatgaat tatctgctgc
    14941 ttaatttttt gcaatcaaat cacaattatt acagaactag attagttgta tacaacaact
    15001 aatctagttg taatttatac atcaagacct aactctttga ggacatcagc atactttctg
    15061 acaacctcag catcttgtag ctcaatcaga agccttttct ctagatcaac atagctttcc
    15121 cctcttgcag ctttttggct tgcaaatgga tcatggtgag ctagcttatt ggcaaagaac
    15181 tgaatttgct tatctgagag cttcttaggc ttcgtatgag taacttcctt aggttggtgc
    15241 ttagcagtga atgtaaatga gaaaccactg atcgtacgcc ccactttatg ttgttcgtaa
    15301 gaagctgtga tatctgtcag ttcattgatt tggtttaaag ctatgtctaa tactcgaacc
    15361 ttaaagttcg ccatctttgc atattcacct tcagctaacc caagtttgaa tcgaaagtca
    15421 tcaatatcaa acataggtgt ctttccaaca ctacgccatt ggatcagtaa ctcgtaaagg
    15481 cgaatcgcat acttacttgt aagagagcta atttgcttca gttcgtaagc agtgaatgac
    15541 ttctcgagct gggtaatcag tggaataacg tcaggcgcaa aagtgagttc tacgatacct
    15601 gcagaatcaa cataggctat tttggaaacc caacgtgatt tcacgatctt ttcctgttga
    15661 gttccctcat gaatactctt gtaactgaat ttgcgctcaa agaggttact agcagcgttt
    15721 ttaagccctt cataggctgc atgcttatca acgttaaatt gcttcatgta atgatgagca
    15781 tgaacctcta acgtgctatt agcgtccaca tccttgctta cttctcttgc ctgcacaatc
    15841 gccaagagaa tcaatcgttg ttctgttgtg tctaaattat agctagcatt gattaacgca
    15901 ttatccttca ctatgaggtc atttttcact gaggtttccc catgtaatta tctaattagc
    15961 atacatcaac aaacagtgta ggcacaaggt gggaaaatgt agacttaaag gtgggaaaac
    16021 gtagacttat gggggaaaac gtagacttat gggtgggaaa acgtagactt aaaggtggga
    16081 aaacgtagac ttatcacctc tgaaacgctt gctatataag gcttacagag gtcttaaaaa
    16141 taaaaagatt aaaagtattt aaaaataaaa agattactgc ttcaaaattt aagagaattt
    16201 cttgttttac gagtatcaat ttacagtgat gaatcagtat gaagaataac catcatttag
    16261 gtgggaaaat gtagactaat gctgaatcgg tagataacag aatttctcaa ctaatcattt
    16321 acaccactta aaaatgcaac tatattgcat tcaaattagt tacaaattac aagtaaaatg
    16381 attctattaa ttggaagcca aaaaggtgga tgtggtaaat caacaatagc catcaacatt
    16441 gcagcatatc ttgctaaaca taacaaagac attgtgttag ttgatgctga tcctcagcaa
    16501 agttcagcta attgggttaa agacagagat gacactaact tacctaaagt gcattgtgtt
    16561 caacgctatg gcgacatcaa gaacacattg aaagacctag atagccgtta tgattatgtg
    16621 attgtcgatg ttgctggaca tgacagcaaa gagcttagaa cagcaatgtt aacggctcat
    16681 tacctggtag taccatttag accgtcacaa ttcgatcttg ataccttacc tcacctatct
    16741 gaagtcatag atcaagccaa gtcatttaat gaaggcttga aaccatttgg cttactaaca
    16801 ttggcaccca caaatccatc agtgtctgaa gtacaacaag ccaacgatta tcttgctgac
    16861 ttcccattgt tcacagcctt gtcatcagta atccatgatc gcaaggttta ccgtgatgtc
    16921 actgctgaag gtaagggtgt tgttgaatcc aataatccaa aggcaataga agagtttgag
    16981 tcattcatga aggagctttt agcatgatta agaaaagaga aattgcgaag ccttcatctc
    17041 ctgacatgga agccttcatc aatggtgcta acgaggaaac tactgaaaaa ctagatccga
    17101 aagcacctag aaaatttaag acgcatacga ttccaatgaa cgaatatgag tatcagttgc
    17161 tcactgagtt ggctgaaaag tatggccaaa cccataatgg aattatcaga tttgctttga
    17221 agaaacttag tgaagaatag caactaattt agaaccaaat taaaaccata atagtttcaa
    17281 gttagctgag cgttgaatgt aaaaaagccc ttaatcatta agggcttttt tgtatctgtt
    17341 agacctatat tttgtctaga atactgcgat tgatgtgtct agcatagatc ttccatctgg
    17401 tataaaacat cacagcttct tccactaaat cttgacggct tatacccaga aattccttga
    17461 tagaattaag atgactatca agctcagggc taagatacac agtagacttt gttcgcttac
    17521 tggttggaag taagcgtttt gcatatagct ctatagctgc gataacagcc cgttctatta
    17581 tctcctcttc tgatatgtca gagtctattt gagagaattc aactaaattt tgcataacaa
    17641 attccttttg ggtttgaggc aatccttgcc taataattac gtttatcgag catcattgct
    17701 tcgacggata caagtataga agaactcaaa taaccatacc aaatttcatt tgataggtag
    17761 ttgattaggc gaaaaggtga gagcaataga tcggcagtaa gcagtaaatg atggaaccaa
    17821 gtgccaaggc ctaatcccct tatctagatg cagttagaaa gtagtcccat gtactttcat
    17881 ccaaagatca aaggcttcta gcatgatgtc tctgtgagtc atgtccttgt ccaaagcatt
    17941 tagcctaagt tgtttatagc gatgttcatc catttttact gttaagtgct ttgttccaat
    18001 gatattggtc tcagtgatct ctatcaatgt tgtctggtaa ggtgtttgat tagccatttt
    18061 gtgctctgta cttactgaaa atagagcgca ttaatactat taaacatcct tttctgtaaa
    18121 gctgaatttt gttttaagta attgataagt aagtacttac gtgcgtacgc acatgatttt
    18181 tagctaatct ttatcatcca aatagggcaa cccaataggt gccctacact gctattttct
    18241 tctattggct cagttaagct ttgaagagat tctctcgatg ccaagcaaga aaaggtaaat
    18301 gctgagcatc cagttttctc agcttgagcg atgatgaaag gttgagtgct gataaatcag
    18361 cagggcttaa ttctgaacta atcatgattg aaccatcatc attaaagcta atgtaccctt
    18421 tatcaaatac tgcatctaaa tttggtgaaa gtaataagcc gttgtaagga tttaacctgg
    18481 aatcctcttg ttcactccac gggataatat gtgaagcgat gagaagtttt tcaaatgtgc
    18541 agtccgatac tgaacaccct tgccagtatt tcaaaagctc ccgtctaaag ttactttgac
    18601 ctaatctaac ctttaccaat gcattaagct cagtcgaagg caaaagagct tcttcttcag
    18661 aaattacagc tttatcactc acatccgaat cttgcctaag atatggaatg atctcattag
    18721 atgtactggc attgattttg agtactaact tacagaatgc ttgcactgct gaatgatctg
    18781 taaagttgaa tgtaatggcc tgaccagaat tcttcggatt aggtggaaat cctgcaaaac
    18841 ttgtttttga ctcaatattc tcattaaggt agatgccatg agcagaaagg ctaattttgc
    18901 ccaacgcgcc aaaaatgtaa agttagcatt tacagcttgt aagccattgt tctagtttca
    18961 caacggcaaa ttgatgttaa gtctaaaaga ccgtgataac tcattgtgga agattgagag
    19021 ctaaatgtaa caatagagga cattgttagt ccaagattta aggggtgaat atgtcacccc
    19081 taagataaaa catttacagc catatctaat acctgcaagg tgcaaaaata tcgagttggc
    19141 tctgatagac ggtggttttt acattcataa gtcctagcaa actgtcgaac aaattgtcgt
    19201 gggaaaagcc gccctgttct gctcgctgtg caacacaagt catgttcaac tgattatctt
    19261 ggctaaagtc attagatacc caagccagca ttggtacgct agtttgttct ttcggtgcaa
    19321 tactataggg cgcaccatgt aaatacatgc ccttttcacc caaagactca ccatggtcag
    19381 agagatacag cattgcagta tcgaacatat cctgcttacc ttttaattta ttcaccactt
    19441 cactgagaat aaaatccgta tatagaatag tgttgtcgta ggtgttaatc agttcttctt
    19501 gactgcaatt ttgaatatca ctgcgcggac aatccggtat aaatttacga tgctctggcg
    19561 ggtatctaag ataataagtt ggtccatgac taccaatgat atgcaaaaaa attactgtat
    19621 cttgacttgg tgctactgcc agaattttat ctaatttgtt gagcaatact tggtcaaaac
    19681 aatattggcc agaacacagc ttcggatcac tcttcaaatc tatcgtgaga ttttcaacct
    19741 gatcacacac acctttacag ccagaatcat tatcaaacca ctgtactttt ataccactat
    19801 gacttaacac atcaatcact gtgtcttgag cattagcacg gcgaggatca tagtctgccc
    19861 gccccattcg tgaaaacata cagggtagag acaccgccgt ggccgtgccg catgagctag
    19921 tatcgttaaa cgcaatcagc ccctgatttt gggtatgagc attggttggc ttgttatatc
    19981 cataatattg atagctcatt gagcgcgcag tttcacccac aacaaacact aataagttag
    20041 gtttagtgtt cgggttacga ctggcattct tcgcatctag gccaagttgt tggtattcca
    20101 tgggcgtctg caaatagtgc tcattgagat atttagatgc actactgaca aaataggtag
    20161 ggacaatgta acgccttaac tcactgttgt ttcgaccaaa tgcagcataa tcttgatagt
    20221 aaaaaaaggc gactatccca atgccaacga acattagcag cataaaggct aatttatgca
    20281 ataactcctt aaaaaagggc tgataatgaa tatcggcctt ataaataaga tatgacggta
    20341 atagcccagt cagcagtaga ttggtaattg atgcaagatt tacatacatc aatgcttcag
    20401 caggatgtgt ttgaaacgtg ttttctatca tgccgtagtc aaacacgaca ttgtattgat
    20461 aggctgcaaa aaatacactt gaggaaagta acgtcaatac gataaaaaag ggcttcagca
    20521 ggtatttgac ggtaaaaatt gaaaacaaaa aactcagcgc aaatgttaaa aatagcggca
    20581 tagatgcgat gaaaagggga tcaacttctg gttgtttttc aattcctttt cgcactatac
    20641 caaagagcgg tagattgaaa atggcaacat aaaacaacgc agtgatgaaa gtgaattggt
    20701 taaccgataa cgtcttaaat ctagaaatca caaaaatccc ttttttgcta aaaaaacaat
    20761 actagcttga cctcattaaa ataaccttat gattaaaagg ttattttaat gagtcaccaa
    20821 caaacttaac agataagacc gttttaggtg aattgagccc aattcaccta aaagttacat
    20881 gtaaacaatt aagtaaataa acttcaatta cttataagtt tacttgtaat ttcttgtgtg
    20941 ctcttcacag aattcagtgc ccgataaata gaggcttctc caaggtgata ctccttggca
    21001 agctgcccaa tggtggcccc ctcagctctt ttatcataaa tagcctgttt taaggtatcc
    21061 gtgagtttta caggtctacc aaatttaacc ccattttcat gtgcttttgc gataccttcg
    21121 gcctgtctct ctgtccgcaa gtcattttca aactcagcaa tcgcagccaa catattgaac
    21181 atgagtctcc cagtcgaagt gctcgtatca atgttctgat cgataacgag taaatcaatg
    21241 ccttcacttt gaaaacgcga agcaatctgg gctagtgtac gactgagcga gcaagacgat
    21301 cgagtcgtgt taccactagg gtatcccctt cacgtaaata actcatgcaa gattgaaatt
    21361 cagaccgttc agccgtcctc ccactgcgtt tttcttgata tatcttattg cactcagctc
    21421 gatgtaactt gcccagttgc acttctaagc tttgacccgt agaactcact cgagcataac
    21481 caactttgga cataaattac catatctctt taaaggtttt gataatgcac ttatgatagt
    21541 agttttgata ggcaataacc agagctgtca aatagtatac attttgataa ggtgcatttt
    21601 tgacttttat cgcccaactg gtgtaaattg agcgaatcga acgattcgtt taaaggtgat
    21661 aaatgaacac tctttcagcc aatgaagcaa aaattcactt tggagatttg ttgctcaaag
    21721 ctcaacaagc tccaatccaa attaataaaa atggcaagcc tgtggccgtt gttatttcag
    21781 ctgatgcgta ccaaagtatc gagacactaa aattacattt gcttcaatcc aaagcggtga
    21841 aagccatgac tgacatcaaa atgggcaatt tggttgatgg taataccttt tttgatgagc
    21901 tggccgcagg gcagtatgac taatgccggt aacttatcat ttaacgcccg atgcacaatc
    21961 tgatctgata ggtattcacc gctttacgtt agcccaatgg ggaacgactc agtcgaagac
    22021 atatttatca ggacttagac aaacgattca gctgctggct gaaacaccca cccttgggaa
    22081 aaatagaccc gaggtacgcg tgaatgtgtt tagcttccct tattcaagtc atgtcatcta
    22141 ttacatccaa catgagcatc aattcgttgt atttgggatc ttacataaaa gtatggttcc
    22201 acttgcacat ctcgcggagc gggaaattat ttgatgtatt gaaatagggg ggcaccatga
    22261 aaaatgacgt tcggcggcta tctattttgt caactgatga aatagatgaa ctgtttggat
    22321 tacctcattt ctcagatgat gatcgtcgat tatattttga cttaagtgct aaagagcgcg
    22381 agctattcga caatacccga acgttttctg tggcagccca tttagtattg caactgggct
    22441 atttcaaagc caaacggcag tttttcagtt atgaacaaga atcgtcagta ctaaatgact
    22501 tggattacat tgccgcgctg tactttccca ccaaaacgct ttcaaggctg aaaagtccct
    22561 caagaccgat tagggctgaa caacaacgag caattcttga tttattccag tataaacaat
    22621 gtgataatga ggtaaaagtt gatttggaag ataaagcaca gcgggttgcg atgctatcga
    22681 cacaacctat ctttatcttt cgtgaattaa cccaatactt agcattacac cgtattgtca
    22741 tgcccagtta tcggtatatg caagagatga ttggtagagt ggtggcttat gaacgcaccc
    22801 gtattgctcg attacttagc acttgcatga cttcacttat cgatcagcaa ttagcggctt
    22861 tgcttcgggc tgattcagga gtatttcgcg tcagcgcatt aaaacatgaa gctaaagatt
    22921 tcagttataa agaattgcga catgaagtcg cacggcggca gtttttccag cctctacatg
    22981 agtttgccaa gcagttcctc ataacagcgg ggatctccaa tgaaagcggc aagtattatg
    23041 cttctatggt taagttttat accacttata aacttcaacg catgaaaaaa gagactgctc
    23101 agttatatct gctgtttttt gcttttcatc ggtttcagca aattaatgac aacttaattg
    23161 aagcattgct ccattgggtc gatcaatacg agaaacaggc caagcgtgcc gctgaagaag
    23221 caatgaataa tgcggttacc aatgcagcga aaaatttaca ggctgcgggt catgtattga
    23281 gcctgtttac ggatgacacc atcaccgatg acacaccttt ttccattatt aatgaaaaag
    23341 cctatgcatt gcttgaacaa gagagattcc cattagttgc tgattactta cgcaatattg
    23401 ctttcgacaa aacggcattt gaatggttac attacacaaa attatccgcc acattcaaac
    23461 gtaacttaag gcaacttttt actgatctgg attttgccgg acgtgtagaa gactctcctt
    23521 tgcttgaagc tatcggtttt ttacaaaact tgttacgcac agaaaaatca ccaaggcaaa
    23581 ctgatcctaa tgcatttccg actgagatta ttcctaaagg tttacgccga tatttgttta
    23641 gtaaagaggg taaaacattt aaaacgctag atgtagatcg ctatgagttt ttggtctatc
    23701 gcctactacg caactcactg gaagcgggtg atgtgtacgt taaacatagt aatgaatttc
    23761 gccgctttga agatgactta ataagcgatc ttagatggca ggataaagaa cgagtattgc
    23821 aagatattgg tgcacccatt ttattggccc ctatccaaga tactctggct gtatttcata
    23881 ctatgctagc agcacgttat agtgcgatta accaacatat ttctgatggg gtcaacaaac
    23941 atattaaagt aattggggct gccgaaaagc gtcgttggaa gctgctttat ccgagcagtg
    24001 atgaatccgt taa
//
