LOCUS       CP075579               20721 bp    DNA     circular BCT 17-SEP-2021
DEFINITION  Staphylococcus aureus strain ER15890.3A plasmid pER15890_3A.1,
            complete sequence.
ACCESSION   CP075579
VERSION     CP075579.1
DBLINK      BioProject: PRJNA470993
            BioSample: SAMN19128535
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20721)
  AUTHORS   Gitman,M.R., Alburquerque,B., Chung,M., van de Guchte,A.,
            Sullivan,M.J., Obla,A., Polanco,J., Oussenko,I., Smith,M.L.,
            Samaroo,F., Barackman,D., Altman,D.R., Sordillo,E.M. and van
            Bakel,H.
  TITLE     Modified methicillin-resistant Staphylococcus aureus detected in
            neonatal intensive care patients
  JOURNAL   J Antimicrob Chemother (2021) In press
   PUBMED   34368846
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20721)
  AUTHORS   Gitman,M.R., Alburquerque,B., Sullivan,M.J. and van Bakel,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAY-2021) Genetics and Genomic Sciences, Icahn School
            of Medicine, One Gustave L. Levy Place Box 1498, New York, NY 10029,
            USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2.3.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 306.519466961x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/20/2021 12:33:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,776
            CDSs (total)                      :: 2,694
            Genes (coding)                    :: 2,628
            CDSs (with protein)               :: 2,628
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 37 of 66
            Pseudo Genes (incomplete)         :: 28 of 66
            Pseudo Genes (internal stop)      :: 16 of 66
            Pseudo Genes (multiple problems)  :: 15 of 66
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..20721
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="ER15890.3A"
                     /isolation_source="nares"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pER15890_3A.1"
                     /geo_loc_name="USA:New York City"
                     /lat_lon="40.7903114 N 73.9528738 W"
                     /collection_date="2019-01"
                     /collected_by="Mount Sinai Hospital"
     gene            1..975
                     /locus_tag="KIS27_13765"
     CDS             1..975
                     /locus_tag="KIS27_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="UAW85490.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKTTLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(1095..1910)
                     /locus_tag="KIS27_13770"
     CDS             complement(1095..1910)
                     /locus_tag="KIS27_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="UAW85491.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(1903..3346)
                     /locus_tag="KIS27_13775"
                     /pseudo=""
     CDS             complement(1903..3346)
                     /locus_tag="KIS27_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(3318..3911)
                     /locus_tag="KIS27_13780"
     CDS             complement(3318..3911)
                     /locus_tag="KIS27_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAW85492.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(4175..4555)
                     /gene="blaI"
                     /locus_tag="KIS27_13785"
     ARG             complement(4179..4555)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(4545..6302)
                     /gene="blaR1"
                     /locus_tag="KIS27_13790"
     ARG             complement(4549..6302)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            6409..7254
                     /gene="blaZ"
                     /locus_tag="KIS27_13795"
     ARG             6410..7251
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7623..8231
                     /locus_tag="KIS27_13800"
     CDS             7623..8231
                     /locus_tag="KIS27_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="UAW85498.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            8645..8938
                     /locus_tag="KIS27_13805"
     CDS             8645..8938
                     /locus_tag="KIS27_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="UAW85496.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            9013..10947
                     /locus_tag="KIS27_13810"
     CDS             9013..10947
                     /locus_tag="KIS27_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="UAW85497.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            10951..11262
                     /locus_tag="KIS27_13815"
     CDS             10951..11262
                     /locus_tag="KIS27_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="UAW85480.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            11274..11900
                     /locus_tag="KIS27_13820"
     CDS             11274..11900
                     /locus_tag="KIS27_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="UAW85481.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            12138..12528
                     /locus_tag="KIS27_13825"
                     /pseudo=""
     CDS             12138..12528
                     /locus_tag="KIS27_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /name="plasmid recombination protein"
     gene            12468..12767
                     /locus_tag="KIS27_13830"
     CDS             12468..12767
                     /locus_tag="KIS27_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UAW85482.1"
                     /translation="MEEEYGKENLLYATVHMDEITPHMHYGVVPITKDGRLSAKEVVGN
                     KKALTEFQDRFNTYINKQGYDLKRGISRQLTKEKHDQVSRYKQKTEYHKQMHMR"
                     /name="plasmid recombination protein"
     gene            12861..13397
                     /locus_tag="KIS27_13835"
     CDS             12861..13397
                     /locus_tag="KIS27_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="UAW85499.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDSYTL"
                     /name="recombinase"
     gene            13782..14642
                     /locus_tag="KIS27_13840"
     CDS             13782..14642
                     /locus_tag="KIS27_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UAW85483.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            14729..15070
                     /locus_tag="KIS27_13845"
     CDS             14729..15070
                     /locus_tag="KIS27_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UAW85484.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(15309..15380)
                     /locus_tag="KIS27_13850"
     CDS             complement(15309..15380)
                     /locus_tag="KIS27_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UAW85500.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            15529..15714
                     /locus_tag="KIS27_13855"
     CDS             15529..15714
                     /locus_tag="KIS27_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UAW85485.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(15933..16676)
                     /locus_tag="KIS27_13860"
     CDS             complement(15933..16676)
                     /locus_tag="KIS27_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="UAW85486.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEPKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            17087..17763
                     /locus_tag="KIS27_13865"
                     /pseudo=""
     CDS             17087..17763
                     /locus_tag="KIS27_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            19266..19883
                     /gene="cadD"
                     /locus_tag="KIS27_13870"
     ARG             19267..19880
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            19902..20249
                     /locus_tag="KIS27_13875"
     CDS             19902..20249
                     /locus_tag="KIS27_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UAW85488.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="helix-turn-helix domain-containing protein"
     gene            20418..20510
                     /locus_tag="KIS27_13880"
     CDS             20418..20510
                     /locus_tag="KIS27_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="UAW85489.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     ARG             6410..7254
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             3645..13938
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          18114..18261
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          13783..14642
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(15934..16676)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          12139..12413
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 atgagtaata ctaaaattaa atatcaaatt tttatttcat caacttattt agatttaata
       61 gaagagcgtc aagcagtagt agaagcagtt ttaaacagta ataatattcc agcaggtatg
      121 gaacttttta actcaagtag taaaagccaa tgggaaataa taacacaatg gatcaatgaa
      181 tcagatgcag tgatattatt gttaggttct agttatggaa caattgatag tgaagaaaat
      241 attagctata cagaaatgga gtacaatttt gctaaatctt taggtaaacc tatatatgtt
      301 tttgagatga ataaagcatc taccgaatcc gcggaaaagt ctaaaaaatt agatgagttt
      361 aagacaaaag ttaaagaaaa tagactaatt agtccaaata ttaatgatat ttcggaatta
      421 aaaagtaaag tatctcagac tttaatgcaa agtgtaccta gtgaaattaa agaaaatagt
      481 ggatggataa gagtacagga ttgtggtaaa aatgtaaaaa aattaaaaaa attggatgct
      541 atacaaagag atttagatac atatgatttt ttgaaagaag aactttataa aatattcatg
      601 tatgtaaaaa ctttttattt ccagcacaca ccatttgatg atagttatat tgaagatata
      661 gaaaactacc tgctaaatta tgaaaataat ccacataaat cattcatgaa aataaaagtt
      721 tatgattata aaaacttttt aaaacattta aaaaacatat tgattttaat taaacaagga
      781 aaaaagccat tagctcaagc cggtcatttt aaattaagct tttacgaaga ggatttaaaa
      841 ttgttacaaa aaaattataa gactacgcta gtcgatatta aaggattgaa taaacatttt
      901 tataaggaat gtgataggtg cttaaaagca tataatattt ttattgaggg tataaataaa
      961 catttatatg aatgatataa gtacacccta atcatacata tataattgtt gtcactttac
     1021 tcgatatatg agaatgactt tacctaataa atgagaaaag caacgcacta tattaaaatg
     1081 cgttgctttt tctattatcg aatacctata actaaactat ctctagctgt ttctacaact
     1141 tctgtactta ttttatctaa accatttata tccataattc tgtctatctg cgcaaataaa
     1201 cgatgaatca gcctaaaatt cccttttgta attttaataa tcgtcgttat tgcctcataa
     1261 tcagtaaagt cttcgagttt taaatcaaat cctaagtctt gccacttata ttctaatata
     1321 tgatgcgtct catctttact tagattatca aattcatgcg caaaacctat acgcgaatat
     1381 agttgaggat aacgagatag ttttttctct atacccggca taccaataaa tatcattgct
     1441 aaattatgtt catcataaat agctcttaat tgctctaaat gttgcacttt aaggcgatca
     1501 atctcatcta ctataattaa atcaatatat ttaaacatat cactataatg tttactatgt
     1561 tcatgtccat acttatttac aacatgtaat ttttgaccca aatcaatgat tgcagtaata
     1621 ctatataaat cattccttaa tttagtttgt ttttctgctg gtgcattata aaatatttta
     1681 tccaccgata gtatgtcatc ggtcgtttta ctagctaaat cttcccaacc tctatggtta
     1741 acttgttttt caatagtatc ccaatttgtg taatacctag acgataaagt ttttccgaca
     1801 ccaggttgac cataacatat accaatatac tgatatttaa tacaagcatc acaaaattca
     1861 gcaaagcgtt tatactcttt cgtttcaatg aaattttgat ttttagtcat tacgatatct
     1921 ctttaatttt ttgacgtttt tagtcgtttc ttgaggtgaa taaccacaat ttttttcttc
     1981 cttaattaat tccgtggtac ttggactagc aatattttgt tttaagtgtt ttcttctctg
     2041 actacgagcg tgctgtatgt cttttatatc tatggagtaa tcagctattt ctggacatat
     2101 agcagtacaa agaaattcat ctctataaaa cacacgtatt tcagccatat catttgggtt
     2161 ataacgaatc agtacatatt cacctacata agcttctaga ttagtattac tgtatcgaaa
     2221 accttgaaaa tgaatgccat ctgaatgaac ttttctagat ttaggtattt ccaacaataa
     2281 taaatcaagt tgttctaagc tacttggcat attagggaaa aagtgattac tattccaatg
     2341 actgataggc gtggtatgaa tagtactatg ttctttttga ttataatctt caattaaaaa
     2401 gtaacgtaat ttttcttcaa aattgtgaaa atttattaga ttactaggag tatcattatt
     2461 gtttatatat ccaggcagtt gttctaagaa agtttgatta actgtctgaa agaatcgttc
     2521 tatttttcca cgaccacgag gaacaccaac tttcgaaaac atcaagttaa ttttcaaatc
     2581 aatagcgact tgttccatat gatgtgaagt aaagtcactt ccatgatctg tgtagaattt
     2641 ttcgggtata ccacacactg gccaattggt atcattttta ttccatattg cttggtgtag
     2701 tgttaatgct gtgttttgag cattgggggg catcaaaact taagaaatag cctgcaattg
     2761 cacgactata gtcatccata ataatagtta gccatggtct attgatatta cctttttggt
     2821 ctaatataaa gatatctagc aaagtatggt ctgcttgcca tatttcattg ggtctcgagg
     2881 attctctaat ttgtattaaa tcgtacttat tttgatagta tttttcaccc tgatgagaga
     2941 aatcaataac agacttaggc atcgctttaa taacactata aatttgttta taacttggta
     3001 tatcaaaatt attttcttta cagtaattag tgattttcct atgaattgtg gctatactat
     3061 ttcttttatt cataagcgca aggcgttcaa tttcatcaat tatctttggt tctattttac
     3121 gtgttccttt atcagctctt gtcgcataga ttagtccttt aagtccttgt tctttatatg
     3181 acttattcca cctataaagg gtgcttaacg caatcccttt acttttagaa atacttgata
     3241 aagcttgttc gcctaggata aaaggtttta taatattgta cttttgagta gcttttttac
     3301 gtttggcttc agaaaaatta gttagatact tctccttgtt tttcatcttc cttgtcctcc
     3361 aatttattga ggtaacgata aattgttgta cgtgaaacat gccactgttc tgcaattgtc
     3421 ttaattggcg tgccattatc ataaagggtc ttaagtaagt ttaaatcttt ttgatttaat
     3481 ttttctggtc tgcctccata tcggcctcta gcacgtgctg caattcggcc agctgaagaa
     3541 cgctctaaaa ttaaattacg ttcaaattct gcaaatgctg cgaataaatg aaataataat
     3601 tgtccagttg aacttgattt atccattgtt atattttctt ctaaactatg gaaacttaca
     3661 cctcgttcgt taagctcatt aactaatgtg attaaatcct ccatattacg tcccaatcta
     3721 tctaatctcc aaacaacaat cgtatctcct gacctcgcaa attcaattgc tttatctaaa
     3781 ccaggtcttt tactttttga accactcata tggtcattaa atatcttttc acaaccatat
     3841 gtatttaatc gatcctcctg taaatttaaa ttttgtaatc cagttgaaac tcttgcataa
     3901 cctattttca aaatacaaac ctcctatata catcttcagt gtaccataac ttaattacaa
     3961 ggtcttttat gaacatagat tttttgttgg gtttttgaac acataatcct atgtaattct
     4021 tacttattta gacataaaag aagagtgtac agaaaatggt tattttctgt acactctcat
     4081 ctttaataat atattttaaa tgtcattaac aaaactaatt taataagagt caagcatagt
     4141 ttacatatgt attatgtttg aaacatacct ctaattactt tttactaata tcatttaaaa
     4201 tatctcgcaa ttcttcaatt tctttgttat ttaattcttc atttttcgca aaattcaaca
     4261 ctaaactttt catatcccct ccatacagtt tattaagaaa ggttttagca gttttcattt
     4321 taatatcgtc ttctttaata tttgatgagt aaaaataaat attttctgat ttgtatcgtt
     4381 ttataatctc ttttttatat agtcttgtga ttaatgttct aatcgtttta tcactaactt
     4441 ctttatattt ttgaatttca actacaattt cattagctga tactgatttt ttatcccata
     4501 ttatattcat aacatcccat tcagccatag atatttcaac ttgcttattg gccatttaaa
     4561 acacccattc cttttaatat tttttcactt attaattcag catttttccc agacgggttt
     4621 ccatctgata aatgtgtagc aaaataatac ttatcatgat ttgtaattac gtaacctaca
     4681 aaccacccat tattatactt cccgtttact atacctgtac ctgttttccc atacagttca
     4741 tacttttcat ttttcttaat caataatgaa gaagataatt gattctttgc ttttttacta
     4801 aaatggttat tttgttccat catattttta aaaactatta cttgttcaag attagatatt
     4861 ttcaaactat cttccatcca atagctttta taacttccca aattttcatt accataattt
     4921 aattgcttga gttgagccgc agtatagttc tttggtattt gattactaat acgttcgaag
     4981 taccaattaa ctgaattttg cattgctgta tttaaatctt gttccttatt ccaagactcg
     5041 aaaagataat gtttatgatt ccatgacata cgtgaatttt tatcacttat aatatgacgg
     5101 tcaagtccaa acagtgctaa ataaattttg taagttgaat caggagaata tctttttcta
     5161 ctttcttttt cattataaat atagtatttg tctttcttca tgctgtacat aacgaaagat
     5221 ccactatttg aaccaaaatt tttactttca tctaaaattt ggtaatcgct ttgtaagggc
     5281 tttttataat tataatcagt taaggattgc cccattaaaa actggctttg aattatcatg
     5341 ataaaaaaag taaaaataca aataaaaatt agaattaatt ttgactgctt tttcagatcg
     5401 gcttctttta tattaattaa tctatttttg agtaatgatt ttttaccatt aaatgaatgg
     5461 cttaatatat ttttgttgaa aaatgaggtt tttaatacgg agtccataat agattccgca
     5521 taagttttaa attcattttt gttaatatta ttaaggacaa atctatcggc ttctacttca
     5581 ttgtcatgaa ttatctttct ttttacaata tgtactaatg gattgtaact cataataata
     5641 gagaagatat taaagataat taaatgtaaa gtatctctat ttttagcatg agcatattca
     5701 tgcagtatta tatattttag tcttttatca attacacttt taaaatatga actaggaatc
     5761 aaaataatat atttcccata ccaaaaagtt attggagatt gaatagcctc tgcttttcga
     5821 atcacaatat tttttttata ttgatggttg aaaagtatcg tatctatttt atttttttca
     5881 ttttcgttta gataaagtga ctgtttcttt aaatatttaa gatataataa ggctttcaaa
     5941 aatttaaaac ttaaaataat aactaaaact atccaaataa ctgtgcagat attatcaatt
     6001 gaatcccaat taaacttatg gatatctgtt gcgaactctt gaacaggttt ggtggtaatt
     6061 atgttatggt tcaagtcgtg tgacttactt tcaactgtgg gcgcttgatt attcacatta
     6121 ttaaatttta taaaagagaa tttaattggg ataaaaggaa ttaatccagc aagcacagtt
     6181 agataccaaa ctttataatt taaactataa ttaaaatagc gttttaatat gtacctaaaa
     6241 aatactaaca atagaaatat aaaacaaaag cttactacac tcattattag taatttagcc
     6301 attttgacac cttctttcaa atatttataa taaacaattg acatcaatat tacaattgta
     6361 atattattgt tttgtaaaaa ttacaactgt aatatcggag ggtttatttt gaaaaagtta
     6421 atatttttaa ttgtaatggc tttagtttta agtgcatgta attcaaacag ttcacatgcc
     6481 aaagagttaa atgatttaga aaaaaaatat aatgctcata ttggtgttta tgctttagat
     6541 actaaaagtg gtaaggaagt aaaatttaat tcagataaga gatttgccta tgcttcgact
     6601 tcaaaagcga taaatagtgc tattttgtta gaacaagtac cttataataa gttaaataaa
     6661 aaagtacata ttaacaaaga tgatatagtt gcttattctc ctattttaga aaaatatgta
     6721 ggaaaagaca tcactttaaa agaacttatt gaggcttcaa tgacatatag tgataataca
     6781 gcaaacaata aaattataaa agaaatcggt ggaatcaaaa aagttaaaca acgtctaaaa
     6841 gaactaggag ataaagtaac aaatccagtt agatatgaga tagaattaaa ttactattcg
     6901 ccaaagagca aaaaagatac ttcaacgcct gctgctttcg gcaagacttt aaataaactt
     6961 atcgcaaatg gaaaattaag caaaaaaaat aaaaatttct tacttgattt aatgtttaat
     7021 aataaaaacg gagacacttt aattaaagat ggtgttccaa aagactataa ggttgctgat
     7081 aaaagtggtc aagcaataac atatgcttct agaaatgatg ttgcttttgt ttatcctaag
     7141 ggccaatctg aacctattgt tttagtcatt tttacgaata aagacaataa aagtgataag
     7201 ccaaatgata agttgataag tgaaaccgcc aagagtgtaa tgaaggaatt ttaatattct
     7261 aaatgcataa taaatactga taacatctta tagtttgtat tatattttct attatcgttg
     7321 acatgtataa ttttgatatc aaaacctgat tttcccttta ttattttcga gatttatttt
     7381 cttaattctc tttaacaaac tagaaatatt gtatatacaa aaaatcataa ataatagatg
     7441 aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac gtatcttatt taaagtgcgt
     7501 tgcttttttc tcatttataa ggttaaataa ttctcatata tcaagcaaag tgacataatt
     7561 gttgaattta tagtaactga aacgtatgat tagggtgtat attaattatg agatggaaat
     7621 caatgattgt tggctatgcg agagtatcat cgaacgatca aaatttagaa agacaacttg
     7681 aaaatttaaa gacgttttgt gctgaaaaaa tatttacaga aaaacagtct gggaaatcta
     7741 ttgaaaatag accagtactt caagaagcac ttaacttttt gaggatggga gatcggtttg
     7801 tagtagaatc cattgattgt ttaggtcgta attatgatga agtgattcat accgttaatt
     7861 atttaaaaga caaagaagta caattaatga ttactagctt acctatgatg aatgaagtgg
     7921 ttggcaatcc attactggat aagtttatga aagatttaat tattcaaata ttagcaatgg
     7981 tttcagaaca agagagaaat gaaagtaaac gtcgacaagc gcaaggaatt caagttgcga
     8041 aggaaaaagg cgtatataaa gggcgcccct tactttattc accaaatgct aaagatccac
     8101 agaaacgtat tatttatcat cgcgttgtag aaatgttaga agaaggccaa tcaattagta
     8161 aaattgcgaa agaagtaaat ataacaaggc agacgattta cagaattaaa aacgataatg
     8221 atttgattta ataaactttg tttcaatgca actgagagct tctcacagcg tctcaaaaca
     8281 gatgattata atctattatg agtttttaat aataagacaa gaaaagagag cgtaaatatg
     8341 ctctctcttt ttctcattaa cgaatgttta taacgcaatc atgatgagag tattaataca
     8401 gttaattatt tgaaatactt tagtttatat aatgttgaat tataggtagt gaaattgtat
     8461 ttattataca ttgtcatatc gttgtttaat gatagttaat ttaaggataa tagaattaat
     8521 attatatata gatgaacaaa acaagcaaca gagcagaaaa aacactttta gattattttt
     8581 actcattctc tattttattt atttttgcta taataaataa agaatattaa atggaggtgt
     8641 ttttatgaaa aagaaatttg tttctagttg tattgctagt acaatcttat tcggcacttt
     8701 attaggtgta acttataaag ctgaagctgc aacagtacat gtagctggtg gtgtttggag
     8761 ccatggtatt ggaaaacatt acgtatggtc ttattatagt cacaataaaa gaaatcatgg
     8821 ttcaacagca gtagggaaat attcatcttt tagtggtgtt gctcgacctg gtgttcaatc
     8881 taaggcatct gctccaaagg cttggggtgg aaacaaaaca ttttatagtc ttcactaatt
     8941 acatatatta attaaggaga atagacagaa ttaagtaaac ttaattctgt ctattctctt
     9001 gaaagaggca atatgaaaaa attcaaatta tttttagatt ttagtacttt attattaatt
     9061 tcagggctat tatttttatt tttcttcaaa gaaaatgaag aaattatacc tgaaagttcc
     9121 aatattttaa caatctctaa ttgggataaa tctaacagta aatcaaaagt gttagatgtc
     9181 attgaatcag gcgccaaaaa tcaaaatata caaataatta aatccgttaa agattttgat
     9241 aataaaaaag aattttttgt tttcaactca aaacgaaata attctgactt tatacgtaat
     9301 aaaacatcac ttttaactcc atcagattta ttaaacagag agataaaagg aaagtattat
     9361 attattggtg aacatttcaa tgtagaagag cttacgagtg aattaaaaaa agctggttta
     9421 acatctgaag ttgaatacat aaatagaaac atattatttt ttgaattagt catagataat
     9481 gatttattag tgcctttcat attcttaggc gtattatatc ttttgtactt cttatatgac
     9541 agaggatata atttgaaatt ttatgctatt caaaagctac atggcttttc aactacacgc
     9601 attatttttc ataatattca tataaaaatc atttattggc tagtacttac cgctattttc
     9661 tttattgcta atatattaat cattcatata gccaatttag aattggattt cttaatgttt
     9721 attcgtagat ttatcgtgtt gctttttgtt tttgcttgta ttactacatt gctgtggctg
     9781 atttcgtata gtcttctttt gaagttaagt attccattaa tcttaaaagg gaaaaaagga
     9841 tataagtttt caatatttat gggtaccttc accaaatgta tcatggtgct aatactatct
     9901 tttttactgg cacaaaattt aaatgcttat acgaagttaa agaatatata tgactctgaa
     9961 aaaatctggc aaaagttaga taattactat acattagaga tttctcctaa tattagaaat
    10021 tcagaagaaa aacaaatttt agaaactaat atatacaatt taattaagaa aagcgaacaa
    10081 cttggtggat tgcttttaaa gaataataat atagccaatc ccgataaaaa caattacatt
    10141 cctgaaaatg gcaatgtctt ttttatcaat aacaattttc tgaatttcta taaaaatata
    10201 aatcatgatt ttaaaatgat tgacaatcat tcggataaaa taaacgtgtt tattcctcca
    10261 aggttacaat atcaaaaatc tgaaattcaa aaaaatcacc aaggatggat tgactttcaa
    10321 aagcaacaaa acaaaaaagt agatgtgcaa gtactgtcaa aaaacgtatc tgttttttct
    10381 tttgatcgca caactaattt gaaatttggg tatctagatt ctccaatagt aatgttgttg
    10441 acacttgacg atttaacagg agatttttat ttagctgctg tttctcaagg tggatatcta
    10501 tttaaagatt tagatacact taaacagttg cttaaagata atcacctaga agaagaaatt
    10561 agcggtataa ctaattacaa ggattctgtg ttaaatgaat taaatgaaac gaaaacaaaa
    10621 atgataataa caatagttac tattattatt aacgttttca ttcttttgat agctactgta
    10681 tttgaaacaa ttcaatattt tagctggaat aaaaagcaac tattattaag aaaaattcat
    10741 ggttattcat tattcagcag taatgtacgt tatttaacaa tttctatctt actttctatt
    10801 atgttagctt acactacgca tattttattt ggttcaaaga tattactatt cataataatg
    10861 tctattgcta ttattcaaca tttacttcaa attgcttata ttaaatatct tgaaaaatac
    10921 tttaaagatt taatgaggga gatttaaaca atgaaaagaa ctttaattat tgtaacaatt
    10981 gtactattaa tcatcatact tagtctgtta ttggttagaa atgagcattt agatcgtttt
    11041 aacccactgt taaaagaaaa agtatcttat gccaaagttc caaaagatac ccaggactat
    11101 agaaatataa ctgtatactc taaaaatgga gaaaaaaaga gttataaatt ggatttttta
    11161 ggttatgatc ctacaaaaga atatgtgaaa gtaaatcata aagggaaata tgtgcgttcg
    11221 atagaataca ttacaaaaga tattcctagc tttttaaaat gaggtgaccg atagtgataa
    11281 aacttgaaaa tgtttttgta aaaaaaggta ataaaaacat tttagacgga tgtaacttta
    11341 attttgaaaa aggaaaatca tatgctttag ttggagaaag tggcgctgga aaatctactt
    11401 tacttaacat aatcgctggg ttcgaagatg tatcacaagg aagcatatat attgaagata
    11461 agttactaaa aaagaaagtc gatttttata gatatacgtt agggtatttg tttcaaaatt
    11521 ttgctttact tgaaaatcaa acaatcagtc aaaatttaga tttagcccta aagttcaaaa
    11581 agaataaaaa agataatatg aacttaaaga aagaagtatt aaaaaaggtc ggcctagatt
    11641 tagatatcaa gagaattgtt agttctctaa gcggtggtga acaacaaaga gtagcattgg
    11701 caagattaat attaaaagat ccgaaaatca ttttagctga tgaacctact gggtcactag
    11761 atacgaaaaa tggaaaaata gttattgatt tattattgaa gctacttgat gaaaataaaa
    11821 ctatgattgt cgtaacgcat gatttagagc ttgcgaaacg atttgatgtc atagtcaata
    11881 tttctgagct tagaagttaa gaaatattga taggtatagc catacataga gaaaaaacaa
    11941 tatgaaatat gattagtcat tacttttgag ttttttaatt taaggactgg attttccagt
    12001 ccttttttat gatgacaaaa aacaagcctc gcagagcaca cgtttttaat gacttatttg
    12061 gtttttaata agtctagtat gttaaaactt ttctgtgttt aggtgttata atcatatgca
    12121 gtggaagttg atttattatg tcttattcaa ttattcgagt tgttaaagtg aaatcaaaaa
    12181 caaatacaag aggtattcaa agacatattc aaagagaaaa taaaaattat gaaaatatag
    12241 atattgattt aagtaagtca tatttaaact atgatttagt caatgatact aagtttgatt
    12301 ttaataaaaa aattgatgag aaaatcgaaa aaaattataa agggaaacgc aaaattagaa
    12361 cagatgccat taaacatatt gatggattaa ttacatcaga taatgtgttt tttaatcaat
    12421 tatctgaaga agagacaaaa tagtttttta agcatgctaa agcgttcttg gaagaagaat
    12481 acggtaaaga aaatctttta tatgcgacag tacatatgga tgagataaca ccacatatgc
    12541 attatggcgt agttcctatt actaaagatg gacgtttaag tgcgaaagaa gttgtaggta
    12601 ataaaaaagc attaaccgaa ttccaagata gattcaatac gtatattaat aagcaaggct
    12661 atgatttaaa acgaggaatt tctagacaac ttacaaaaga aaaacatgat caagttagcc
    12721 gttataaaca aaaaacagaa tatcataaac aaatgcatat gcgctagaag caaatagagg
    12781 attatttaaa gcaagaaaat gataaattga tacaaaacta ccaaaaatca ttagatacgc
    12841 ttaaaaagct tctgagtgtc atgtatgaaa ttaaaactaa aaatgtaggt ggatggtttc
    12901 ataaagagaa gcaagaaaca ggcaatattg taattactaa aactgatttt gaaaaataca
    12961 ctaaacaaat taaagcagca cagatgatat tagatgatta tgaatgtatt aaaagtggga
    13021 agtcattaaa aaaagcagag aaacaaaatg aaagtttagt taatgaattg actagtgtcc
    13081 atatggaaaa tgaaaagctg gttgaagaat ttaatgattt agcacaacgt tataattatt
    13141 tgttgtcaga gaatgaaaag aaagataaag aactaaatta tacgcttaag ttattcaatc
    13201 aagtttttaa aatcattaaa agtatgatga aagaagagcg ttatcataca ctaataaatc
    13261 atattgataa tcatttagac aattctaaaa tacgtgaagt aatgacgata gataacaatg
    13321 atgaacagtt ctttaagaag aaatatcaag cacaagaacg agaaatcatt tttaaagaag
    13381 atagttatac attataaaaa tagatattta aatgcatttg tttgaggtta attatttttt
    13441 aggatgaaaa ataggtagcc cccctcgaac tttttaatat atttttataa atctttttaa
    13501 aaaccttttt aggggcttgt tcagccctag agcaacaagg gatttcaatg cctaaaggag
    13561 tgtttttgcc acataaaagg agtgtttttg ccacataaaa ggagtgtttt tgccacataa
    13621 aaggagtgtt tttgccacat aaaaggagtg ttttaaaaaa gttgtactcc ttttatggtt
    13681 tgacattgat aataaagtag tttattgtga ataaaagttt tcaaatggga gtgtatattc
    13741 aaacgatact cctttaggtt cgtaataaag ggggctgtat catgacagga gagacagttg
    13801 tatataaaaa tgaaatgaat ttagttccat taaggcgatt tacagctact gaaattaatt
    13861 tattttttgc gatgtgtaat aaattaaaag agcaagatac taatacatta cgtttgtctt
    13921 ttgatgaatt aaaaaaatta agtaattata gtccagaaac acgtaatatt aatagatttg
    13981 ctaatgattt agataatgtg tataaaaaga tgttgaattt aaccattaga tatgaagatg
    14041 atgatgtaat agaaagattc gttttattca atcattatag aatccataag cgagaacaat
    14101 atctagaaat atcaacttct tcaaatttaa aacacatatt gaattcaatt actaataatt
    14161 ttacaaaatt tgaattaaaa gaaatgacta ggcttaaatc tacatattct aaaaatatgt
    14221 ttagattact taaacaatac aaacatacag gctatttaaa aattcacatc gatgatttta
    14281 aaaatcgttt agatatacct aaatcttatc gtatgactga tattaacaaa aatgttttta
    14341 aacctattat tattgaatta ggttctatat ttaataatct tactatcaat aaaatcaaag
    14401 cgaaaaaagg gcgtaaaatt gaatggatag agttcacatt tgacgctgag aaacgcattc
    14461 ataacaaacg acaacctcaa atgtccaaga tagataaatc aagacaatat gtgaggcgtg
    14521 aaaagacgcc taaatggtta gaagaacggt catatgaaaa gcaacctcaa aaagattacg
    14581 acccacaatt agagaaagaa cgagaggatt ttttaaaaca acttgaactg aattgggaat
    14641 aacgcaactt ttcgttgcga gttggttaac taaaagtatc gtgacttttc aattatttta
    14701 attatagttt gagttaagga gtgattttat gaaattagct gaagctataa aagaacagag
    14761 agaattaaaa ggatggtctc aagaggaatt agccaatata ttaaaggttt ccagacaaag
    14821 tgtttctaaa tgggaaagtg ccaaaaacta tccttcctta gatattttga ttgctatgag
    14881 cgatttattc ggaatctctt tagagcattt aattaaagga gatgcacgtt ttaaaaaagt
    14941 tattttagag ggaaattata gagagagtaa cagggtacct tccatagttg attttcttta
    15001 tgatttttgg tggcttttct ttcctgttgc cggattttta gtttgggcca ttcattcgtt
    15061 catgggttga ttttaaaatc aaaatttaat gcacatcagg caatatgaaa aaggaaagta
    15121 tttttacgac accttgaaat gtattgataa gaatagaaga gtttacaaaa ggcaatatga
    15181 aatatatact aaaagtaaca agaatttcgt ttgtaaacgc acaaaacccc gaactattcg
    15241 ccgtagttcg gggttttttg gtgaattggc tttaatcgtc tatattattt tttatcacgt
    15301 ttatggagct aataaccaaa taacgtaacg agacaaccac tgattgccgt tgtcatgatg
    15361 tgaacaagaa tttcattcat aaatcgcacc tcctctctac gccgaattga catctgagag
    15421 atagacgacc ttcacattat aacaaatctc tccttccatg aaataacatg aatattaata
    15481 aatgttgtta tatattaaag ataaataaat ataagggtgg gattgataat gtttggcgat
    15541 caaagacaag aagctacaaa atatgtcatt aaagaaggtt atcaagatat ttatttcctc
    15601 aacaaaaacg gggaatggta ttatttcgaa gtaagaagtg tctggcgtgg caaacatatt
    15661 attagagtta aagatggttt gttaggttgg agaaaagaaa tagtaactga ataaagatac
    15721 aaaatacttg ccaatgcaaa agaatgaaaa cgcgtcaaat caacgatgta taaaagatgc
    15781 ataaaagata atgaaagtaa aaagccataa atgccgtcat ataggcattt atggcttttt
    15841 gtttggttaa cataatcgat gattatcaga agttcaattc acataaaaaa gcacggctaa
    15901 tttatgaact tgtcgtacct ttttaattaa aattagttac caaatagacg actccaaaac
    15961 ccctttttag gttccgtttc ttcactcttt tcttctatat cactatcatt gtcatttgga
    16021 ttagctgaaa cgtcttctct ttgtgcttct tcaccttgtg tactttcgtc gctttgactt
    16081 ccagatatat ccttatattg aatctctgtt tctttagttt cttcctgatc tcggttaata
    16141 tttacaggac tagtagtata tgttttttct tgtgcatcaa cgttttgtct agcgttagta
    16201 gatgtatcaa agggataatt aaggtgtttt tctttttcta attgattttc taatttttga
    16261 atctttttat tactctctaa tgccagaact tgctgattct caagcaattt tgatctttct
    16321 tgcaactctt ttctgtaaaa ttcaattgtt gtttcatgac gactttcttg tttttcaatt
    16381 tgattattta aagtattaat ctgattttta agtatttcta ctatttgagc atcgctttta
    16441 gattcaaaat taatattatc cgactctttt ttttgtttgt cttcagtagc actttcaaaa
    16501 ccatatttct ttttattttc atttactctt tgaatgattt tttctatatc ggtatcttct
    16561 ttaataaacg aagtgttttc ctgttttatt gtctctatat tcaatctctt aatattatta
    16621 aaaatcgttt gcttactaac ccctaattct tcactcaacc tttttacact tttcatatca
    16681 tcaacccttt ataaaatgtt tataaaccct tagtaaaata ttataagctt ataacgatta
    16741 ataataaagt aatcgacaaa taaaaatata aattcgccaa cctttttatc aagggtttgt
    16801 aaagggttta tggtgtttat caaaggttta taaacctata ttttcccttt tttaagttta
    16861 ttcaaagtgc tataataacc ttaatataac aaaaacccca actactggta atagtcgggg
    16921 tttggtaata aaacgtttag aaactgttta tttatataat tttcgatact tagattatat
    16981 aatgaataat atcttagtgc aagcacctag attattaaat aaacacaaaa aatgtaagcg
    17041 ttttattacc tcttaccaaa ctaaaattcg gaaagaggtt tttattatgt caattaaact
    17101 ttacaacata aatgaggaat acagagaaaa gttttatcaa ataccgaaag tattttttac
    17161 aaatcctaaa tatatcaagt tatccaatga tgcaaaaatg acatggggta ttcttagaga
    17221 tcgtttagat ttatcaatta aaaatggttg ggttgattct aaaacaggta atatttattt
    17281 ttattacaaa aatgaaaacc cacaaaatat attaaattgt aaaaaagata aagtaattaa
    17341 aataaagaaa gaacttaatt gaagccgaat tactactaca agaaagacaa ggtttaaata
    17401 agacaaataa actttattta gttaaaccag aagtaacaga agatgatatt tataaaattc
    17461 aaaagcaaga aaacactgtt gagagcagca atggcaagga agtcggaaaa accgacttca
    17521 agaagtcgat aaaacagaat ctaatgatac tgaacttagc tatactgaat ctaacaatac
    17581 tgaaataaat gatatgaatg acttgaatga tatagagtcc gaaaatgaaa ttagtaatca
    17641 ttcaagtcaa tgcaaggtta tttaatgcaa acaattttaa ctgaatttga agaaacacat
    17701 gcactttata tgcgccgtaa aaacatgaaa caatataata tctttaatca atcattcaaa
    17761 tgattttata acaatcaaag atacatcaaa atatttcgtt aaatcatctg actttttaac
    17821 tgatagcgat gaaaaaatca agaaattgtc agtgatctag aaaaagattt gtatcgaaaa
    17881 cgtatgttaa gttatggtgg attgcttaaa taaaaacata aaattttaaa cttagacgat
    17941 gctgaagatg gcaatttaat taatacaagt gatgaagata aaatagcaaa tgaagaagaa
    18001 aaggcacatt cgattacggc aattttgaaa agcaaaatta ttatttaagg cattagtgat
    18061 aactgatgtc tttttttatt acttttttgt aaaaaaagtg ctgtcttatt tttgtgacaa
    18121 atgctgtatg tagtgtcaca aaagtgtgac acttgagctt tttatgaccc cacacaaaaa
    18181 cattttcgaa gccttgaaat atctggcttt gccagatccc ccattcattg atggggacaa
    18241 atttccctta tgctcttacg gagattttag agttaaatta aaatttctaa gtttttgata
    18301 agaaaagccc tgcaggaatt tagaagaacg tggggaagtt ttaaagaatt ttatgctaat
    18361 ttttaatttg catgtaaatg ggcagtgtct aaaaaatagt tattgttttt gctcaaattt
    18421 ttgtttttga aaatagcata tatttatttg gctcatattt gcgttttaaa agcgtatata
    18481 agttttagat ataaaactat atgaatcacc cctaaatctt taaaatgccc tcttaaaatt
    18541 caaaataagg gcataaaaaa taaaagcccc ccagataaag tgaggtagct ttttctttcg
    18601 acgccgtatc gtcgatgtaa ttcaaaaaag ttatgggcta taaatctaca tcactttttc
    18661 aagaatgtag tagcaatatt caacgcaaat taatttttat aactgctcgg aatatctcaa
    18721 gccgtttctt taattttgaa ataaaaaaat cgacgaaggt cgattacgtt tttgcacatg
    18781 tcgatttatc cgacgtataa gtgcgccctt acgggattta actagattat aacgacgaat
    18841 tttagacctg taaagcaata acaattaggt ctaccaggta gataaagtgt acaaagtaga
    18901 accgaaaaag ttatttattt tgaagatttt tcgtagatat aatttgctaa taaacttcct
    18961 aaaactgaat aaagaaaaat agttaaactc atgtaatcac tccataattc tgaagtgtct
    19021 ccatgtctac gcatagacac gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac
    19081 ccttaactaa acttgcagac gaatgtcggc atagcgtgag ctattaagcc gaccattcga
    19141 caagttttgg gattgttaag ggttccgagg ctcaacgtca ataaagcaat tggaataaag
    19201 cacctatgat tatttaaaaa tataagttat aatacattca aaaatatttt tgaatgaggt
    19261 gtattatgat tcaaacggtt gttgctgctg ctgttcttta tattgctaca gcagttgatt
    19321 tattagtaat tttattaata ttttttgcta gagcaaagac tagaaaagaa tatagaaata
    19381 tttatgttgg tcaatattta gggtctatta ttttaatatt agttagtttg tttttagctt
    19441 ttgtattaaa ttatgttcca gaaaagtgga tattaggttt attgggttta ataccaattt
    19501 atcttggaat taaagtggct atttatgatg attgtgaagg agaaaagaga gctaaaaaag
    19561 aattgaatga aaaaggattg tctaaattag ttggtacggt tgcaattgtt acgatagcaa
    19621 gttgtggcgc cgataatatt ggtttatttg ttccgtattt tgtgacatta agtgttacta
    19681 atttattact tactctgttt gtctttttaa ttttaatttt cttcttggtg tttactgcac
    19741 aaaaattagc taatatccca ggaattggag aaattgttga gaaatttagt cgttggatta
    19801 tggctatcat ttatatagct ttaggtttat ttattattat tgaaaatgac actattcaaa
    19861 caattttagg atttatattt taatttaggg tgtgatttta tatgagttat gaaaatactt
    19921 gtgatgtgat ctgtgtacat gaggataaag ttaacaatgc tttaagtttt ttagaagatg
    19981 ataaatctaa gaaattactt aacattttag aaaaaatttg tgatgagaag aaattgaaaa
    20041 tcatattatc tttgattaaa gaagatgagt tgtgtgtttg tgatatttct ttgatattga
    20101 aaatgagtgt tgcttcaact tcacatcatt taaggctttt atataaaaat gaggtacttg
    20161 atttttataa agatggaaag atggcatatt attttattaa agacgatgaa ataagagagt
    20221 ttttctctaa aaatcaggag ggtttttgaa acgagtgaaa cgagtttctt cttgtcttga
    20281 tactataaaa aacaaagtta tataacataa gacgacttat cggaagttat ctatcatcgt
    20341 atattcggag atgatataat atacaggtcg cctatctctc aggcgtcaat tcagacgcag
    20401 agaggaggtg cattactttg ctaatcattt tcgttcacat catagcacca gtcatcagtg
    20461 gctgtgctgt tgcgtattat acttattggc ttagtaaacg caacaaataa ttaataggcg
    20521 actttataag ccagcatcga aaaaatcccc acactattgc agtagtgtgg ggattggtgc
    20581 attaattgtt aatcattttc gttatctaga ttataacact ggttagtaga ggaatgcaaa
    20641 aagataatat tttaagtagt ctttttggat attatcatat gatatattta tgtaaattga
    20701 ttttttatta aaggagcata t
//
