LOCUS       CP074552                2495 bp    DNA     circular BCT 09-JAN-2023
DEFINITION  Enterobacter cloacae strain S36_CRE37 plasmid pCRE37_4, complete
            sequence.
ACCESSION   CP074552
VERSION     CP074552.1
DBLINK      BioProject: PRJNA667445
            BioSample: SAMN16387654
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 361x
            Sequencing Technology             :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/10/2021 19:39:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,878
            CDSs (total)                      :: 4,766
            Genes (coding)                    :: 4,668
            CDSs (with protein)               :: 4,668
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 34 of 98
            Pseudo Genes (incomplete)         :: 62 of 98
            Pseudo Genes (internal stop)      :: 12 of 98
            Pseudo Genes (multiple problems)  :: 10 of 98
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="S36_CRE37"
                     /isolation_source="urine cc"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="pCRE37_4"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-10-03"
                     /collected_by="Rachel Addison"
     gene            145..336
                     /locus_tag="KHW01_24380"
     CDS             145..336
                     /locus_tag="KHW01_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WBN67969.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(627..812)
                     /locus_tag="KHW01_24385"
     CDS             complement(627..812)
                     /locus_tag="KHW01_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WBN67970.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1178..1405)
                     /locus_tag="KHW01_24390"
     CDS             complement(1178..1405)
                     /locus_tag="KHW01_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WBN67971.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          493..580
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1821..2349)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 gaacccgcct gtaccggttc agcagccgtt ccggcctgac tgcaattttt tttttttcat
       61 ccctgcccgc taccctgtaa acctttcttc tgcgttgccg ttaacagtag gtaagccatg
      121 tttggttttt cttccggagc cctgatgact cttagtgatg atgagcgaca cctgcttgtg
      181 tccgttgttt cggtctggct acggcgtgcc ggaggggatg ccggagccat gatgctggat
      241 gcataccggc agattttgtc tgaaaccgaa ccggccgtaa ggactgtcat gctcgaattt
      301 cttgaatccg ttcgcattca ttacatttcg tcctgagcat tttctgtatt tctgtccata
      361 cttaaacgag gcggccgtca cggacggtgg tcgtcgttcc atgatgctgt tatggacgag
      421 tgagagtaat ctaatctaac ttgattgagt tccgggcgtg ccgcccggtt ttttttgctg
      481 tttcccggtg tccgttccgg gacaggatgt gcatttgtaa taccgcgcac gcgccggcat
      541 cacaaatacc ctggtcaggg ctgcgccccg acaccccgcc atcgcctcac ggccgcgcac
      601 tgaattgcgc acccgttcac cgattttcac gcattttcct cgcctatctg tgcgttcagc
      661 atggcgtaaa gcgcttcggc ctgatcgtgc aggtgctcac agcagccggc agcctcatcc
      721 agatccatct gctcgagccg ctcaaggagg agaagtgtct gtgcgcggat aaatttggcc
      781 atattgaggg cggcggattc ctgtctggtc actggtgatt ttccccgtct ggtgatgatt
      841 tttgcgtgaa aagagcatgg cgtcattctg gtgcatagtc atgcatgccg ttaaaattta
      901 acaggcgcgt ttctgacggg ttccggggtg gtttgttgtg gtttttgtca tggtttcgtc
      961 aggaacgcgc tgaacgcgtc tcaggcggtg cgggcaacgg atgttatggt aaattcttgg
     1021 gggcttgatt ataaaatacc gcacttcctc ttatgtcttg tgctttttag acaaccctga
     1081 aagcttttag ctttggtgat gcaatttgct gggattttgg cggagaacca caggtaaaga
     1141 aaaaggccac attagcggcc tttttcggag aagatgctca gtgcggaatt tcggagactt
     1201 ctttaacgtc ggttttattg atctgctgtt tgacaccatt cgcgtctgta tagccaagca
     1261 ggcctgagtc tgattcttta ggtttgccgt cgctgacaat agtgcgaccg tcattggtgt
     1321 gtatggcata gcttgtgcgc gtgcagccgg tgagggctga aagcgcaact gccgctgcga
     1381 acactgaaac aaaaatcttt ttcaaagcca gctcctttta tccatatcag ttttggtcac
     1441 taaccatcta actataggtc attttttatg tgaaaagagg cttgatggtg ggctgtatat
     1501 cgagttaggc gcgaggtgct atggtcaaag tgtggtgtca ggcggggagg aggcttatat
     1561 gagatgtaat ccggccgata catataccaa gatgatttaa gtattcctct ttgtgaggtt
     1621 ggatgtctaa tttataaagg atcttcttga gatccttttt ttccgcgcgt aatctcttgc
     1681 cctgtaaacg aaaaaaccac cctggcaggt ggtttttcga aggttaggta atcctggcag
     1741 atcccctaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatttgt cctttcagtg
     1801 tagcctcact aaggccgcca cttcaagaac tcttgagaca tctctcgcac atcctgtttg
     1861 ccaatggccg ttgccaatgg cgattagtcg tgtctttccg ggttggactc aagttgatag
     1921 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccatcctg
     1981 gagcgaactg ccttcccgga actgagtgtc aggcgtggaa tgagaaaccg cggccataac
     2041 agcggagtga caccggtaaa ccgaaaggca ggaatgcggg ggagcacgag ggagccacca
     2101 gggggaaacg cctggtatct ttaagccgca tcgggtttcg ccaccactga tttgagcgtc
     2161 agattctgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt ggcgcggcct
     2221 tatgctttct tcgttaagta tcttcctggc atcccccagg aaatttctga tccatccgta
     2281 agcccgtccc gctcgccgca gccgaacgac cgagcggagc gagtcagtga gcgaggaagc
     2341 ggaatatatt ctgtatcaca ttttctcctg acgcgttttc tttcactttc tgcgcctgtc
     2401 ttatgtggca ttaatgctat gtgttactgc catgctacat cttaagccag tatacactcc
     2461 gctagtgctc cgtgactggt ccggcgctgc gcccg
//
