LOCUS       CP074551                5906 bp    DNA     circular BCT 09-JAN-2023
DEFINITION  Enterobacter cloacae strain S36_CRE37 plasmid pCRE37_3, complete
            sequence.
ACCESSION   CP074551
VERSION     CP074551.1
DBLINK      BioProject: PRJNA667445
            BioSample: SAMN16387654
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Characterization Of Recent Carbapenem-Resistant Enterobacteriaceae
            Isolates From A Large Tertiary Care US Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Elam,L.P., Chittur,K., Addison,R.M., Lewis,S., Smith,B.A.,
            Anderson,D.J. and Sysoeva,T.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2021) Biological Sciences, University of Alabama
            in Huntsville, 301 Sparkman Dr, Huntsville, AL 35899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 361x
            Sequencing Technology             :: Illumina NextSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/10/2021 19:39:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,878
            CDSs (total)                      :: 4,766
            Genes (coding)                    :: 4,668
            CDSs (with protein)               :: 4,668
            Genes (RNA)                       :: 112
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 34 of 98
            Pseudo Genes (incomplete)         :: 62 of 98
            Pseudo Genes (internal stop)      :: 12 of 98
            Pseudo Genes (multiple problems)  :: 10 of 98
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="S36_CRE37"
                     /isolation_source="urine cc"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="pCRE37_3"
                     /geo_loc_name="USA: Durham, NC"
                     /lat_lon="36.0075 N 78.9384 W"
                     /collection_date="2017-10-03"
                     /collected_by="Rachel Addison"
     gene            join(5782..5906,1..640)
                     /locus_tag="KHW01_24340"
                     /pseudo=""
     CDS             join(5782..5906,1..640)
                     /locus_tag="KHW01_24340"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            615..1106
                     /locus_tag="KHW01_24345"
     CDS             615..1106
                     /locus_tag="KHW01_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WBN67964.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQNMSEAMEKNRRSVLLTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1113..1328
                     /locus_tag="KHW01_24350"
     CDS             1113..1328
                     /locus_tag="KHW01_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WBN67965.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1886..2338)
                     /locus_tag="KHW01_24355"
     CDS             complement(1886..2338)
                     /locus_tag="KHW01_24355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WBN67966.1"
                     /translation="MPTFTQTGTGKYDYWLLDGGKTFSTIPADTLPSISTDMPIRLQIG
                     DGYFGSTHITARHGKWLERYQPDGCVATFVHKKLSTSGKILLLEEKNKIGLALTLTPNA
                     ALILRNIGDFFSITTLYYKKSGLDGEEVGRYTGYKWATSPYIERRR"
                     /name="hypothetical protein"
     gene            complement(2341..2775)
                     /locus_tag="KHW01_24360"
     CDS             complement(2341..2775)
                     /locus_tag="KHW01_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WBN67967.1"
                     /translation="MKAKTEPLEKKWGERSMLMGWTAIPTSFLFLQAELSITPLGFNIL
                     INLVSHWWETKEHPYPSQESLAKRMGVSKRSIQREVTNLVELGLITKQANKASSAKYKG
                     RNTYDLTGLVERLNEMAPEKITSMQRHKKAPEQPDESGEQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            3817..4062
                     /locus_tag="KHW01_24365"
     CDS             3817..4062
                     /locus_tag="KHW01_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WBN67962.1"
                     /translation="MKNVRMQFDLPEDRLQELDTLMSKCGISTRKELFNYALTMLEWAV
                     DESENGHDIAAIDRAKKEFYSLRMPILKRQVKTASQ"
                     /name="hypothetical protein"
     gene            4132..4536
                     /locus_tag="KHW01_24370"
     CDS             4132..4536
                     /locus_tag="KHW01_24370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WBN67963.1"
                     /translation="MSDPNDLDGFSDATGGKVLVVKDFVGPIMAAEERADRRHLRWSKT
                     GVTFAMTVVVALMNWMVIDMLGTVVQQEMHMISMGVLPAENRTVTTDVYLALIAGTVAE
                     VSALFFIIVKSMFKSSNEPKDTAVTESTST"
                     /name="hypothetical protein"
     gene            5520..5843
                     /locus_tag="KHW01_24375"
     CDS             5520..5843
                     /locus_tag="KHW01_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WBN67968.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          5235..5407
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          complement(4704..5310)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.77"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..1420
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.263"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          5521..5837
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.21e-61"
ORIGIN
        1 gcagcgtgaa ggcgccaccg tcctgcaggg gaagccggaa gaagtccggg agctgattga
       61 cgcgtcgcct tacgttaaga aatacacctc cggcgtcctg tcgtttgcgg aggccgatct
      121 ccctcccggc cagcgggaaa aactgatggc cagcttcgag cgagttctga tgcccggact
      181 cgataaagat cagtacagca ttctgtgggt ggagcacgcg gacaaggggc gtctggagct
      241 gaactttctg atccccaata ccgagctgct gaccggcagg cggctccagc cttactacga
      301 ccgcgccgac cggccgcgca tcgatgcctg gcagactatc gtgaacggca gactggggct
      361 gcacgacccg aacgcgccgg aaaaccgccg ggcgctggtc acgccgtccg ggctgccgaa
      421 aacgaagcag gaggccgcag aagccattac ccggggacta ctgagcttag cctcgtccgg
      481 ggagctgaaa agccgtcagg atgtcactga cactcttgaa ggtgcaggtt ttgaggtggt
      541 gcgcaccacg aaaagcagta tcagcattgc cgacccggac ggggggcgaa acatccgact
      601 gaagggagct atctatgagc aatcttttta cggcctcgag ggacttcgag gagaaattga
      661 aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc attcagcgag cacgagcagt
      721 ctgtcagagc ggaactgagc gcaagcgcga ggagaatcag cgacgccatc agcgcccacg
      781 agcagaatat gagcgaggcg atggagaaaa accggcggag cgtgctgctg acggccggga
      841 ggacatggct caccatcctg atggtgtcag cgctgctgat cggcacgagc gggagcattc
      901 tgtggtggca ggggcagcag ataaccgaca attacacgca tcttcgtcag caggaagata
      961 ccctggcgaa aatgaccgcc cggacgtggg gagtgcgcta tcaggagagc agcgacggcc
     1021 ggagattcct tatcctgccg ccggggatgc aaaccgaagc catcccttac gacgggacga
     1081 catggatccg cctgaaacag gagtagcgga aaatgacaga gctggaaaag cagttgctga
     1141 gcgcattaga gcagctacag caggactact cgcaaaggct ggacgaatgg gagagcgcct
     1201 tcgcggaatg gcggagcatg tgtggtctta tgcaacggga gaacgcgatg ctgaacgagc
     1261 gcgtgtcgga cttgagcacg caggtgctga gtttaagcga gcagctgcgc cgcttgtccg
     1321 ggaactgaat gccatcgagc tccggcagag ggagaatgag tatcagaaaa cactggagct
     1381 ggagcgccag ccgcagaaaa cctaccgcgg gccgacactt taaaccatcc aggcagctga
     1441 caacccgcac gaagaggggc tcttatttct cccgctaaat aggttcgtga tcaaaaaggt
     1501 cacgacttat tcaggagcgg gaaatgaagc tgttaaatct tattgaatca ttgcttaact
     1561 gcctatacct ggcggcggag ctgtgggact ggctggagaa gcatttgtcg gtttttaagg
     1621 cattggcgcg gattttagtg tgactttaac atccatgcat ctggctgcat gactatgcac
     1681 cagtgtaata ttcagcggct gcgtttctgg tgttttttcg ttatgtctgt cgccactttc
     1741 tcgcgtctgg caccggaaac gcagccaacc ccgtttcagt ggtgcgggca acggatgtta
     1801 tggtaaataa cgttgatata aagctggaag tgagtttatt cgatatcggc attatccagt
     1861 ctttatcgct gcctgatttt agaaactagc ggcgtctttc tatgtatggc gatgttgccc
     1921 atttgtaccc tgtatatctc ccaacctctt caccgtccaa tccgctcttc ttgtaataaa
     1981 gagttgttat ggagaagaaa tctccaatat ttcttaagat aagagccgcg ttgggggtta
     2041 acgttaaagc cagtcctatc ttgttttttt cctccagtaa aaggatcttc ccggaagtgc
     2101 ttaatttctt atgaacgaat gtagctacgc atccatcagg ctggtaacgc tcgagccact
     2161 tcccatgtct tgccgttatg tgtgtggacc caaaatagcc atccccaatc tgtagacgta
     2221 tgggcatgtc tgtagatatt gagggaagag tgtctgctgg tatggttgaa aacgtttttc
     2281 ctccatcgag cagccagtag tcatatttcc ctgtcccggt ctgggtaaaa gtgggcattt
     2341 ttattgttcc cctgactcat caggctgttc tggagctttt ttatgtcgtt gcatactcgt
     2401 tatcttttct ggtgccattt cattaaggcg ttcgactaag ccagtaagat cgtaagtgtt
     2461 tcttcccttg tacttagcgc tactggcttt gttagcctgc ttcgttataa gccccaactc
     2521 aactaagttc gttacctccc tctgaatcga gcgcttggat acacccattc ttttagctaa
     2581 tgactcttga gagggatatg gatgctcttt ggtttcccac caatgggaaa ctaggtttat
     2641 caaaatgtta aaccctaaag gtgtgattga aagttctgcc tgaagaaaca ggaaagacgt
     2701 aggaatagct gtccatccca taagcatgct tcgctcaccc cacttcttct ccaatggctc
     2761 agttttagct ttcatgatac ctcagttaat tttttttctt cagaagtgac aacaagcaca
     2821 gggcaacatc ttataaatat aagatgttgc cctgtgcttg tatatattta tatatgtata
     2881 ttcatatgta atgacaatat gtcaccttta aatagcgaca tattgtcacc atacttgcac
     2941 tgagtcttac agaagacgat ttgcccgtca tggcgatatg atggcgaggc gacatgcgct
     3001 gatagtggat cgccctgcga aagcagccac ttgggtgggc tccttcattg cgagggatga
     3061 tgaatctaga taactggaca tcacgtacgc cactcagact cataaaaaaa gctgccctag
     3121 gcagcttttt ttgttagtgc atcatcaatc ttcgtaagag cgacgttttt ttgaccccta
     3181 agtcgcttgt tcccgcaaaa gacaatccta caaaatcgct tcccgactgt ttttataatc
     3241 tattgttttt aaatgatttt attattagtt ttcacttaat gacaaaaaaa tacgcataaa
     3301 acaattgctg tagttgtttt tttggtcatc tctgtgcata ctcattttac cctccgattc
     3361 tactcgacat ttacacaaaa ttagagtagt cttaatatca gctactgaaa aagtttcagt
     3421 agatgtatgg agattctaat cgtgagcaag ttgatttcag ctagctcatc gtcctcggag
     3481 gacagcgagg attgctccga tttcgtagag gtagagtttc aactacctct caaaacgtgg
     3541 gtaatcgatc ctgtagagga agatgactga aactcacctt ggggaaagat gaccgccatc
     3601 gccttttccc caaggtgaga gctacatgct aatcaacgcg atagctggcg atttacacat
     3661 gaccaacgca ttagttagtc ataggtgcac ttgcacccaa aatacagaca gcaataaacc
     3721 aattagcaat taagttattg atataataac aataataatc tcacactgtt tcggctatca
     3781 cccatggacg tggagagata gttgcggaga ttcaacatga aaaacgtacg tatgcaattt
     3841 gaccttccag aagatcggct gcaagagctc gacactctga tgagtaagtg cggcataagt
     3901 actaggaaag agcttttcaa ttacgcctta actatgctcg agtgggctgt tgacgagtca
     3961 gaaaatggcc acgatatcgc tgctattgat agagcgaaaa aagagttcta ctccctacgt
     4021 atgccaatcc tgaaacgcca ggtgaaaact gcctctcagt aacgcagcaa acaacaaaaa
     4081 aggccagatc tatgatccgg ccttttttgt tatttaaagg atagtagttt aatgagtgac
     4141 cctaatgatt tagacggatt ttcagatgct accggcggaa aggtacttgt cgtaaaagac
     4201 tttgttggtc cgattatggc ggcagaagaa agggctgaca gaaggcatct ccgatggtct
     4261 aaaacgggcg taacgtttgc catgactgtg gtcgtcgcgc taatgaactg gatggtcatt
     4321 gatatgcttg gcacggtagt tcagcaagag atgcatatga tttctatggg agtgcttccg
     4381 gcggagaaca gaacagtaac tactgacgtg tatttggctc tgattgcggg tacggttgcc
     4441 gaagtatcag ctttgttttt catcatagtg aaatcaatgt ttaagagtag caatgagcca
     4501 aaagatacgg ccgtaacaga gagcacttcg acgtgatcat gacggacgta ttgagatttt
     4561 aagttagccg ctgtagtctc ttgtccataa acgaaaaaac cacctgggga ggtggttttt
     4621 cgagggttca gcaagttggg gaacttctga accgtggtaa cagggtgtac aagaccgctg
     4681 ccaccaaatt cgtccttcca gtttagccgt agttgggctg atacttcaag aactctgcat
     4741 cagtaatatc ttgtacaccc tcttaccagt ggctgctgcc agtggcgctt tgtcgtgtct
     4801 ttccgggttg gactcaagtt gaaagttacc ggaacaggcg cagcagtcgg gctgaacggg
     4861 gggttcgtgc atacagtcca gcttggagcg aactgccttc ccggaaccga gtgtcaggcg
     4921 tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     4981 caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttat agtcctgtcg
     5041 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     5101 tatggaaaaa cggcttcgcc gtggccttat cgcttccctg ttaagctttt tcctggcatc
     5161 tcccaggaaa tttccgcccc gcctgtaagc cggtaccgct cgccgcagcc gaacgaccga
     5221 gcgcagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     5281 ctccgctgct gcatttttct cctgccacat gaagcacttc actgacatcc gcattcgtgc
     5341 caacatagta agccagtata cactccgcta acgctacgtg actggttcag ggctgcgccc
     5401 cgaaacccgc caaaagcctg acgcgcctgc ggcttgtcca accccgcgcc gaccgggaaa
     5461 ggctttcccg cccgtcccgg cgttatcagg aggccggttt gtcagagaaa cggaacaaga
     5521 tgctcaccat gtgggtgacg gaggacgagc accggcggct gcttgagcgc tgcgacggca
     5581 ggcagctggc agcgtggatg cggcagacct gcctggacga aaagccggca cgttccggaa
     5641 agcttccctc aatctcgccg gcgctgctgc gccagcttgc cggcatgggc aataacctga
     5701 accagatagc ccgccgtgtt aacgccggcg ggggtaccgg ccatgacagg gtgcagattg
     5761 ttgcggcgct gatggccatc gatgccggac ttgagcggct gcggcacgcc gttcttgaga
     5821 aaggaacgga cgatgatcgt taaatttcat ccacgcggtc gcggcggtgg cgccgggccg
     5881 gtggactatc tgctgggtaa agaccg
//
