LOCUS       CP073309               12242 bp    DNA     circular BCT 28-APR-2023
DEFINITION  Klebsiella michiganensis strain CZ598 plasmid pCZ598_GES7, complete
            sequence.
ACCESSION   CP073309
VERSION     CP073309.1
DBLINK      BioProject: PRJNA722384
            BioSample: SAMN18753391
KEYWORDS    .
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12242)
  AUTHORS   Finianos,M., Kraftova,L., Papagiannitsis,C.C., Adamkova,V.,
            Hrabak,J. and Bitar,I.
  TITLE     Genomic characterisation of three GES-producing Enterobacterales
            isolated in the Czech Republic
  JOURNAL   J Glob Antimicrob Resist 29, 116-119 (2022)
   PUBMED   35231657
REFERENCE   2  (bases 1 to 12242)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 560X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2021 13:51:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,187
            CDSs (total)                      :: 6,072
            Genes (coding)                    :: 5,925
            CDSs (with protein)               :: 5,925
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 49 of 147
            Pseudo Genes (incomplete)         :: 101 of 147
            Pseudo Genes (internal stop)      :: 24 of 147
            Pseudo Genes (multiple problems)  :: 18 of 147
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12242
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="CZ598"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="pCZ598_GES7"
                     /geo_loc_name="Czech Republic: Pilsen"
                     /lat_lon="49.73 N 13.37 E"
                     /collection_date="2019-12-02"
                     /collected_by="FNP"
     gene            complement(join(11530..12242,1..88))
                     /gene="blaOXA"
                     /locus_tag="KDU73_30860"
                     /allele="blaOXA-1011"
     CDS             complement(join(11530..12242,1..88))
                     /gene="blaOXA"
                     /locus_tag="KDU73_30860"
                     /allele="blaOXA-1011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_034033256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase OXA-1011"
                     /protein_id="QUG49855.1"
                     /translation="MTIRIFAIFFSTLIFATFAHAQEGTLERSDWKKFFDDYQAKGTIV
                     VADERQADRILSVFDQARATKRYSPASTFKIPHTLFALDVGAVRDEFQVFRWDGIKRSF
                     AGHNQDQNLRSAMRNSTLWVYELFAREIGEEKAKRYLKQIGYGNADPSTSKGDYWIDGT
                     LEISAYEQISFLRKLYRNELPFRVEHQRLVKDLMITEAGRNWILRAKTGWEGRFGWWVG
                     WVEWPTGAVFFALNIDTPNRMDDLFKREAIVRAILHSIDALPPN"
                     /name="blaOXA"
     gene            246..1259
                     /gene="intI1"
                     /locus_tag="KDU73_30865"
     CDS             246..1259
                     /gene="intI1"
                     /locus_tag="KDU73_30865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="QUG49856.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLQTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     gene            1462..1812
                     /locus_tag="KDU73_30870"
     CDS             1462..1812
                     /locus_tag="KDU73_30870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="QUG49857.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVNQ
                     WRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATETS
                     VKPDACDSPPSG"
                     /name="DUF3330 domain-containing protein"
     gene            3328..3633
                     /locus_tag="KDU73_30875"
     CDS             3328..3633
                     /locus_tag="KDU73_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUG49858.1"
                     /translation="MKDARDDQTLDLLPSGKRRGRPPTGKALSPAAKQAAYRARQREKT
                     VTVTLNRPDCGELEIFLLNLRDGRTSTLAPEVVARLHDAVRSAWLGQLHSGNGDQK"
                     /name="hypothetical protein"
     gene            3630..3971
                     /locus_tag="KDU73_30880"
     CDS             3630..3971
                     /locus_tag="KDU73_30880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005322038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QUG49859.1"
                     /translation="MTPAKRERVIKIRVTDEEFARLKGLSGDERLAEWMRSQCLGHDKA
                     AARRRTPVPKADPALLRQIAGIGNNLNQVARRVNSGEWGAVERVQVIAVLMAMERALQA
                     LSVPSTEVT"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3968..4141
                     /locus_tag="KDU73_30885"
     CDS             3968..4141
                     /locus_tag="KDU73_30885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUG49860.1"
                     /translation="MMNEYGVAEYDYTLIRLPGEQGWSLRLLKDGQEVSGEVYQEHDEA
                     LSVATVWLCSES"
                     /name="hypothetical protein"
     gene            4138..4926
                     /locus_tag="KDU73_30890"
                     /pseudo=""
     CDS             4138..4926
                     /locus_tag="KDU73_30890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899683.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4826..5299
                     /locus_tag="KDU73_30895"
     CDS             4826..5299
                     /locus_tag="KDU73_30895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUG49861.1"
                     /translation="MSKILELAKTFEESSKQQASDIETSVKNAFEPHERAILAALESSA
                     RRLNTAIDAQNRRWGWLVLKGWVFPLIGVAFLLGISWVVVWYQGRVIAENWVEISRQNK
                     TLEQLAAKGGKLELSTCGKDQRLCVKMDLKELAYGREEESERGEYPWRIPEGY"
                     /name="hypothetical protein"
     gene            5300..5524
                     /locus_tag="KDU73_30900"
     CDS             5300..5524
                     /locus_tag="KDU73_30900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QUG49862.1"
                     /translation="MTELERQLLKALESLEGGYRAQHAAWESAYSDLRQMFEATSTENR
                     ALRESVGSLSKQVLHLSGQVETLSQKLKP"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5823..5924)
                     /locus_tag="KDU73_30905"
     CDS             complement(5823..5924)
                     /locus_tag="KDU73_30905"
                     /inference="COORDINATES: protein motif:HMM:NF013676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUG49863.1"
                     /translation="MLWNTIYMQAALTHLHDEGEVINEEDEADDWPL"
                     /name="hypothetical protein"
     gene            6042..6719
                     /locus_tag="KDU73_30910"
     CDS             6042..6719
                     /locus_tag="KDU73_30910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
                     /protein_id="QUG49864.1"
                     /translation="MKKTTLEYTPVDLSRWARKEHFEAFQSFAQSTFNQTVLLDITALL
                     KHTKEVGWKFYPTIIFLLSKIVNNHAEFRMTIKNNELVIWNDIHPSYTIFHNETETFSS
                     LWSHYDGNIHHFHDAYSADMARYGNNLSYSPKDESRENVFFVSAIPWVSFTSFNLNIAN
                     MQNFFAPIFTFGKYYKQDGKVLLPFAVQVHHSVCDGFHVARLVNELQEMSDDILRLSEE
                     IND"
                     /name="type A chloramphenicol O-acetyltransferase"
     gene            complement(7355..8359)
                     /locus_tag="KDU73_30915"
     CDS             complement(7355..8359)
                     /locus_tag="KDU73_30915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS4321 family transposase"
                     /protein_id="QUG49853.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDRRGKAVYRKKFTRPKLIEFLATC
                     PATTIAMEACGGSHFMARKLEELGHSPKLISPQFVRPFVKSNKNDFVDAEAICEAASRP
                     SMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLST
                     LLEDNSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLT
                     ASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCAR
                     VFIQKLEHQSGKLADWVRDLLCRKSNFVVTCALANKLARIAWALTARQQTYVA"
                     /name="IS110-like element IS4321 family transposase"
     gene            complement(8438..8899)
                     /locus_tag="KDU73_30920"
                     /pseudo=""
     CDS             complement(8438..8899)
                     /locus_tag="KDU73_30920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            9106..9729
                     /locus_tag="KDU73_30925"
     CDS             9106..9729
                     /locus_tag="KDU73_30925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="QUG49865.1"
                     /translation="MLIGYMRVSKADGSQSTNLQRDALIAAGVSLAHLYEDLASGRRDD
                     RPGLAACLKALREGDTLIVWKLDRLGRDLRHLINTVHDLTARSVGLKVLTGHGAAVDTT
                     TAAGKLVFGIFAALAEFERELISERTVAGLISARARGRKGGRPFKMTAAKLRLAMASMG
                     QPETKVGDLCEELGITRQTLYRHVSPKGELRPDGVKLLSLGSAA"
                     /name="recombinase family protein"
     gene            complement(9945..10808)
                     /locus_tag="KDU73_30930"
     CDS             complement(9945..10808)
                     /locus_tag="KDU73_30930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase GES-7"
                     /protein_id="QUG49854.1"
                     /translation="MRFIHALLLAGIAHSAYASEKLTFKTDLEKLEREKAAQIGVAIVD
                     PQGEIVAGHRMAQRFAMCSTFKFPLAALVFERIDSGTERGDRKLSYGPDMIVKWSPATE
                     RFLASGHMTVLEAAQAAVQLSDNGATNLLLREIGGPAAMTQYFRKIGDSVSRLDRKEPE
                     MGDNTPGDLRDTTTPIAMARTVAKVLYGGALTSTSTHTIERWLIGNQTGDATLRAGFPK
                     DWVVGEKTGTCANGGRNDIGFFKAQERDYAVAVYTTAPKLSAVERDELVASVGQVITQL
                     ILSTDK"
                     /name="class A extended-spectrum beta-lactamase GES-7"
     gene            complement(10912..11466)
                     /locus_tag="KDU73_30935"
     CDS             complement(10912..11466)
                     /locus_tag="KDU73_30935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ib'"
                     /protein_id="QUG49866.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLAD
                     VQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQSLA
                     NASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTP
                     DGPAVYMVQTRQAFERTRSVA"
                     /name="aminoglycoside N-acetyltransferase AAC(6')-Ib'"
     ARG             complement(9949..10808)
                     /name="blaGES-7"
                     /gene="blaGES-7"
                     /gene_name="extended-spectrum class A beta-lactamase GES-7"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(10916..11466)
                     /name="aac(6')-Ib'"
                     /gene="aac(6')-Ib'"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(6')-Ib'"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(11531..12240)
                     /name="OXA-732"
                     /gene="OXA-732"
                     /gene_name="OXA beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="99.58"
                     /coverage="88.72"
     Typing          4139..5604
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.025"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagaacgttc tagcgtgcct tcttgtgcat gcgcgaaagt ggcaaaaata agagtggaga
       61 aaaatattgc aaagattcgg attgtcatta acttttcctt gacgcccaac tttgttttag
      121 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
      181 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac
      241 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
      301 agttgcgtga gcgcatacgc tacttgcatt acagtttaca aaccgaacag gcttatgtcc
      361 actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca
      421 gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg gtctccacgc
      481 atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc acggatctgc
      541 cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc
      601 cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg ttcgcccagc
      661 ttctgtatgg aacgggcatg cggatcagtg agggtttgca actgcgggtc aaggatctgg
      721 atttcgatca cggcacgatc atcgtgcggg agggcaaggg ctccaaggat cgggccttga
      781 tgttacccga gagcttggca cccagcctgc gcgagcagct gtcgcgtgca cgggcatggt
      841 ggctgaagga ccaggccgag ggccgcagcg gcgttgcgct tcccgacgcc cttgagcgga
      901 agtatccgcg cgccgggcat tcctggccgt ggttctgggt ttttgcgcag cacacgcatt
      961 cgaccgatcc acggagcggt gtcgtgcgtc gccatcacat gtatgaccag acctttcagc
     1021 gcgccttcaa acgtgccgta gaacaagcag gcatcacgaa gcccgccaca ccgcacaccc
     1081 tccgccactc gttcgcgacg gccttgctcc gcagcggtta cgacattcga accgtgcagg
     1141 atctgctcgg ccattccgac gtctctacga cgatgattta cacgcatgtg ctgaaagttg
     1201 gcggtgccgg agtgcgctca ccgcttgatg cgctgccgcc cctcactagt gagaggtagg
     1261 gcagcgcaag tcaatcctgg cggattcact acccctgcgc gaaggccatc ggtgccgcat
     1321 cgaacggccg gttgcggaaa gtcctccctg cgtccgctga tggccggcag cagcccgtcg
     1381 ttgcctgatg gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     1441 agccgtcttc tgaaaacgac aatggaggtg gtagccgagg gtgtggaaac acccgactgc
     1501 cttgcgtggt tgcggcaggc gggttgcgac acggtgcagg gtttcctgtt cgccaggccg
     1561 atgccggcgg cggccttcgt cggcttcgtc aaccaatgga ggaacaccac catgaacgcc
     1621 aatgaaccga gcaccagttg ctgcgtgtgc tgcaaggaaa tcccgctcga tgccgccttc
     1681 acgccggaag gggccgagta cgtggagcat ttctgcgggc tggagtgcta tcagcgcttc
     1741 caggcgcggg ccagcactgc gaccgaaacc agcgtcaaac cggacgcttg tgattcgccg
     1801 ccgtcaggtt gaggcatagc ccaaccgggc gtcagacgaa gcggcgaaag agcgtcagag
     1861 tagggccttt tccggaattt cctgacgcct tcggcgcggc gtctatggcc tcaacctgac
     1921 acgccagccg ttagcgtgct ttattgtccg aattctgagg cgacccctgt aaggcttcgc
     1981 cgagaagaca cttcggcgat ttgggcacca aacacagagg cgtttatctg tgacgagcat
     2041 gcggcttgtg gttttaatgt cgatgttcag ctttctcttc gccaagatca tcagcttgag
     2101 acggctgtat cggctggggg gactgttgcg cggcgtagaa ctccgataac gaatatgccg
     2161 taatcaagac aggatccttt tccatctctt cggatccttc attaaaacgc gctatggcgc
     2221 gttttttgtt cctgctatgg aaatgctatg ggttaggtgg cgagtgagct ctacgctcgt
     2281 tctgtgaggt tcgtattcta aatgatcttg atcagatcct ctgatcaagc gtaacctgcc
     2341 caccagaaaa caaaaaaccc acctatctag gcgggttctt tgcccggtgt tcctcggctt
     2401 gccggccttg gaatccggtg ggtaacagct tcgagcagga agcggatacc gttaagtacc
     2461 ccctcagttt agccgcgtgc ggcccactgt tcaagtatca aacccctgct cgaaccctgt
     2521 taccaaaggc tttcaccttt ggcgctttgt cgtattcgct cggattggac gcaagtgatt
     2581 gttgccgtag cgggtgtgaa ggtcgggcag aacggggaaa tctcacatac tgcccaagcg
     2641 tgcaatcgcg caccacagcc tcccaggact cgcaaccctg gggcatctgc aagtagagcg
     2701 taacggggaa ccaaccacgg ccagctctta aaaaacggcg cggcagcccg accaacaggc
     2761 gggggctaaa gcagagtgca gcgggacggg gtgtgttctc tggagcgcgt gagagggagc
     2821 tgccgcaagc agtctcgata gtccggagca aggcgagctt atcctgtaaa agacctcaaa
     2881 tcgatatggg tctgtaggag ggctctccta tcttgaagcg cagcgaaaga taggagagcc
     2941 cggcaaactg tggcggcttg ccaacatcct tttctcccgt tttttgtttc cccagggcac
     3001 gtatcatcac cccctcgttg agctcttttt gcctcctacc ggttacctac cctcgaagcc
     3061 ctctaattat agtcactgtt actttaacta gtctcgcaaa caggtggtta caacctattc
     3121 cggctatgtc aaacttgact ctaagccagc atacacagag ggcccttagc cgcttcgcgt
     3181 cttcggttcc tctgtgaagc tggttcaggg gcgtgccgcc cccgaaaccc cctgcctcca
     3241 ccgccccgaa aaaccccgtt ccgggctttc ccaggtcggc ggacaggata ggtatatggt
     3301 cacagttacc gtaattgagg tgatcggatg aaggatgcac gagacgacca gacgctggac
     3361 ttgttgccga gtggcaagcg ccggggtcgg ccacctaccg gcaaggcgtt gagccctgcc
     3421 gccaagcaag ctgcttaccg ggcacggcaa cgggaaaaaa cggtcaccgt taccttaaat
     3481 cgtccggatt gtggcgagct ggagatcttc ctgctgaacc tacgggatgg ccgaacctcg
     3541 accttggccc ccgaggtggt ggcccggttg catgatgctg tgcggtcggc gtggctgggg
     3601 cagcttcatt ccggtaacgg tgaccagaaa tgaccccggc caaacgtgaa cgggtgatca
     3661 agatccgagt caccgacgag gaatttgccc ggctcaaggg cttgagtggc gatgaacggc
     3721 tggccgaatg gatgcggagc cagtgtcttg ggcacgacaa ggccgccgct cgacggcgga
     3781 caccggtacc caaagccgat ccggctctgc tgaggcagat agccgggatc ggcaacaacc
     3841 tcaaccaggt agcgcggcga gtgaatagcg gggagtgggg tgcggttgaa cgtgtccagg
     3901 tgatcgctgt gctgatggcg atggagcgtg ctttgcaggc cctgtctgtc ccctcaacgg
     3961 aggtaacgtg atgaacgaat acggcgtagc cgagtatgac tacaccctga tccggttgcc
     4021 cggcgaacag gggtggagct tgcggttgtt gaaggacggc caggaggtca gcggggaggt
     4081 ttaccaggag cacgatgaag cgctcagtgt ggccaccgtc tggctgtgca gcgagtcatg
     4141 atcgtcaaat ttcatgcccg tggtgtgggg cgcggtagcg gcccggtcga ttacctgctg
     4201 gggccgcagc gggatcggga gggggcgacc cttgaccgtg gcgatccggg tctcatcgag
     4261 gcgctgatcg acagcagccc ctacgccaaa aagtacacct ctggcgtgct gtcgttccag
     4321 gaagcggatt tgcccaggga ggacaaggac aagctgatgg acagcttcga gcgtgcgtta
     4381 ttgccggggc tggatcggga tcagtacgcc tgcctgtggg tggagcaccg ggacaaagaa
     4441 cggcttgagc tcaacttcgt gatcccgaat atcgaactgc tgagtggcaa gcggctgcaa
     4501 ccctatttcg accgggccga ccggccccgc atcgatgcgt ggaaggtggt ggtcaacgcc
     4561 cagttgggct tgcatgaccc ggatgatccc ttgaacaaac gggcattgat gacccccagc
     4621 aacttgccaa aagccaaaca ggacgcggca caagcgatca ccgacggtct gctttcactg
     4681 gcggcggccg gtgagctggc cgaccgggga gacgtggtgg ccacgttgaa gcaacacggc
     4741 tttgaggtgg tgcgggagac ccgcaagagt atcagcattg ccgacccgga aggggggcgg
     4801 aatatccggc taaaaggggc cctgtatgag caagatttta gagctggcca agaccttcga
     4861 agagagctcg aagcagcagg cgagcgatat cgaaacgagc gtgaagaacg cgttcgagcc
     4921 gcacgagaga gctattctcg cggcgttgga atcaagcgcg cggagattga acaccgctat
     4981 cgacgcccag aaccggaggt ggggctggct ggtgttgaag gggtgggtct ttccgttgat
     5041 cggggtggcg tttctgctgg ggatcagctg ggtcgtggtg tggtaccagg gcagggtcat
     5101 cgccgagaac tgggtagaga tctcacgcca gaacaagaca ttggagcagt tggcagcgaa
     5161 gggcggaaaa ttggaactgt cgacgtgcgg caaggaccag cggctgtgcg tgaagatgga
     5221 cttgaaggag ttggcctatg gccgcgaaga ggaatcagag agaggggaat atccctggag
     5281 gatcccggag gggtactgaa tgacggaact agagcggcag ttattgaaag cgttagagag
     5341 cttggaaggc gggtacagag cgcagcacgc ggcctgggag agcgcctaca gcgatttgcg
     5401 gcagatgttc gaggccacct ctaccgagaa ccgggcattg cgagagagcg tggggagctt
     5461 gagcaagcag gtgttgcact tgagcggtca agtcgagaca ttgagccaaa aattgaagcc
     5521 ctgaacattg agctggagcc gcggcgtagc tctatgagcc tcgggcggga gtatcgagag
     5581 tatggtgaca gggagtacgg tgattgagtg gggtcagtgg gccccacttc attgttgatg
     5641 cccctgttag gggttgtacg ccggaatccc aatttcggcg gttccggctg atccaagaat
     5701 cttggcgtga ccttacctcc gctctaccgc tgggtgcggg gtctgaagcc caaccgtacg
     5761 gaaacgtacg ctaagtatgt tttttaacca ctatgatgac gtaagcaaac cgcttcattt
     5821 cattataacg gccagtcatc cgcttcatct tcttcattaa taacttcgcc ctcatcatgc
     5881 aaatgtgtca gtgcggcctg catataaata gtattccaga gcacgacgga attccctctc
     5941 gcataaaaat gtgctattat aaaaataaat tggtttccat gtattattac taattcataa
     6001 aaaccgcagt ctcacatcta atatagactt tataattaaa tatgaaaaaa acaaccctag
     6061 aatatacacc tgttgactta tctcgttggg caaggaagga acactttgag gcctttcagt
     6121 cctttgctca atctacattt aaccaaactg tcctgttaga tattactgca ctactaaaac
     6181 acacaaagga agttggctgg aaattttacc ctactattat tttcctcctg tctaaaatcg
     6241 taaataatca tgcggaattt cgtatgacca taaagaacaa tgagctcgtc atatggaatg
     6301 acattcatcc aagctataca attttccata atgaaacgga gaccttttca tcattatgga
     6361 gtcactacga tggcaatatt catcacttcc atgacgctta ttcagcagac atggcacgct
     6421 atggtaataa cctttcttat tcacctaaag atgagtctcg ggaaaatgta tttttcgtat
     6481 cagctattcc ttgggtaagt tttaccagtt ttaatctcaa tatagctaat atgcagaact
     6541 tttttgcccc catttttact tttgggaaat actacaaaca ggatgggaaa gtattgttgc
     6601 ctttcgccgt tcaggtacat cattccgtgt gcgatggttt ccatgtggca agactggtca
     6661 atgagttaca agagatgagc gatgatatac tgcgtctttc agaggaaatc aatgattgat
     6721 gcgctatatg gtattgacgc gaaaatggga tttggggagc aacggaacaa taaacgccgt
     6781 taaatggtca ttttaaatag ccaaaaatca accggccaat ttacatcgct tattgccatt
     6841 tttagccgcc tcttttacga tagccaaatt aactatactc atcttggcag tttgcggagc
     6901 aaaagacgca attgatctca agagattgat ttacctccta ttgaccattc aatttaaccg
     6961 cttaaccgtc tttttggttc cactactccc caaaccccta tatccaatat gccccccttt
     7021 agggagttcg aagcccaaag gaacggaaac gtacgctaag agcgtaacct tctgtttggg
     7081 gtcgcttcag aaaacggaat ctatggtcac tcccgttttt gcaacaccga ttttgacgac
     7141 aagttggctt gcttgaatct atccggcgtc tgaatgggat tttattcccg cgccttgatg
     7201 agttccgcgc ctgatgaacc tccagaaaat atacggcttc aatgagcctt tccgttttac
     7261 aggttcctca acaggccggt gggccgttag tatcatcaat atcagtattc gcaaaaccag
     7321 atcagtaatt ctttaaaccg gtgtatttct gccgttatgc tacataagtt tgctgtcgtg
     7381 ccgttagggc ccaggctatt ctggccagct tgtttgccag agcacaagtg acgacaaagt
     7441 tgcttttccg gcacagtaaa tccctgaccc aatcggccaa tttgccagac tggtgttcca
     7501 gtttttgtat gaataccctg gcacattgaa ccaacaaagt tcggatcttt ttattacctc
     7561 gcttactaat tcccagcaat gtcgtcctac ctcccgtgct gtactgccga ggtacaagcc
     7621 ctgttgccgc cgcaaagtca cggctgctgg cgtactgctt cccgtcgcca atctcagttg
     7681 aaatagtact cgctgtcagt gttccgacgc agggaatgct cagcaagcgc tgtccaacct
     7741 catcttcgtc caactttcgt ttcaactggg attccaaatc tttaatctgc tcaacaagat
     7801 agtgataatg ctgttgtaat ttcagcaata actggctgag gtaaagaggc aaactattgt
     7861 cctcaagaag ggtactcagt cggctaataa cggcagctcc tcggggaacg ctaatgccaa
     7921 attccagcag aaaagcatgc atttgattgg ttgtttttac cttatcctga accagggatt
     7981 cacggacacg atgcagagcc cgcattgcct gctgagattc cgttctgggc tgcacaaaac
     8041 gcatagacgg acgcgatgca gcttcacaaa tagcttcggc gtcgacaaag tcgtttttat
     8101 tgcttttaac gaacgggcgg acaaattgtg gtgatatcag ctttggggaa tgccccaact
     8161 cttccaactt gcgtgccata aagtgagaac cgccacaggc ttccattgcg atggttgtag
     8221 cggggcatgt cgccaaaaat tcgatcaact ttggccgggt aaatttttta cggtaaacag
     8281 ccttcccgcg acgatcctgg caatgaatat ggaaagagtt tttacccaga tcgataccaa
     8341 tgagcgcaat gttttccatg atagttctcc gaatgaaagc ctgtcctcag cataataccg
     8401 ggaaggaggg agtgaccatc tcattaaaaa tcgtacgcta agacccggag aacggacgta
     8461 gtggcctgaa cttgcccgcg tctaccttgg cgctgctgcg ccagaggtaa tcgccggtca
     8521 agttgatgtg ttcccagccc agcggcgaca ggtactgcaa cagcgcctca tcgacggcat
     8581 ggccgttgcc acgcaaggca tgcgctgcgc gctccaggta gactgtgttc cacaacacga
     8641 cagccgccgt caccaggttg agtccactgg cccgatagcg ctgctgctcg aaactgcggt
     8701 cgcggatttc gcccagccgg ttgaagaaca ccgccctggc cagcgcgttg cgcgcttcgc
     8761 ccttgttcaa cccggcatgc acgcgacgac gcagttcgac gctttgcagc cagtccagga
     8821 tgaacagcgt gcgctcgatg cggcccagct cgcgcagtgc cacggccagg ccgttttggc
     8881 gtgggtagct gccgagcttg ctgtcatttt cagaagacga ctgcacccaa tgtcagaagt
     8941 cgattgcacc tctggaaatg acaatcgaat ttctgtcaca ttaggttcct gatacaccct
     9001 ttgcatgcct tagactatat tacgggttaa gtgacaaatt gtgatgcccg ggcctttctg
     9061 ccgcaggctg tcacttaatc gaacgtatac gtgacgggtg aaaaggtgct gatcggctac
     9121 atgcgggtat cgaaggcgga cggatcccag tccaccaatt tgcaacgcga tgcgctcatc
     9181 gccgctggtg tgagccttgc gcacctttac gaggatctgg cctcgggcag gcgcgatgat
     9241 cgcccagggt tggctgcttg cctgaaggcg cttcgtgaag gggacacgct gatcgtgtgg
     9301 aagctcgatc ggcttggccg tgatctgcgc cacctgatca acaccgtgca cgacctaact
     9361 gcgcgtagcg tgggcctgaa ggtcctgacc ggtcacggtg cggcggtcga cacgacgact
     9421 gccgccggca agcttgtgtt cggtattttt gccgcgctgg ccgagttcga gcgtgagttg
     9481 atttccgagc gaacagtcgc tggacttatc tcggcgcgcg ctcgcggcag gaaagggggg
     9541 cgccccttca agatgaccgc cgccaagcta cgcctggcga tggccagcat ggggcaaccg
     9601 gaaaccaagg tgggcgatct ctgcgaagaa ctcgggatta cccggcagac gctctaccgg
     9661 cacgtgtcgc ccaagggcga actgcggcca gacggcgtaa agctgctctc cctcggttca
     9721 gccgcataaa tggaggcgac ctggaacggg gcgctgttca gtgcggcaac gatccgatta
     9781 ccggtgtcga cccagagcag ccgtagagct tttgggaaag ctgtcgttca acgcctgagt
     9841 taagccgcgg tgcgcagtgg caagcggcgt gcgaagctta gcgtagcacg caagcgccgc
     9901 cacgaagcaa cgtcggcttg aacgaattgt tagacgggcg tcaactattt gtccgtgctc
     9961 aggatgagtt gtgtaataac ttgaccgaca gaggcaacta attcgtcacg ttctacggcc
    10021 gatagtttcg gggccgttgt atacaccgct acagcgtaat ctctctcctg ggctttaaaa
    10081 aaaccaatgt cgttccggcc cccgttggcg caggtaccag ttttctctcc aacaacccaa
    10141 tctttaggaa aacccgctcg tagtgtcgcg tctcccgttt ggtttccgat cagccacctc
    10201 tcaatggtgt gggtcgaggt ggacgtcagt gcgccgccat agaggacttt agccacagta
    10261 cgtgccatag caataggcgt agttgtatct ctgaggtcgc caggtgtgtt gtcgcccatc
    10321 tccggctctt tccggtctag ccgactcaca gagtcgccaa ttttacgaaa atactgcgtc
    10381 attgcagcag gtccgccaat ttctctcagt aagaggttag tagccccatt gtcgctaagc
    10441 tgcaccgcag cttgcgctgc ctcgagaacc gtcatgtgtc ccgatgctag aaaccgctcc
    10501 gtggcaggag accatttgac gatcatgtcc ggcccatatg aaagttttcg atccccccgc
    10561 tcggtgcctg agtcaattct ttcaaagacc agcgcggcta gcggaaactt gaacgttgag
    10621 cacattgcaa aacgctgcgc cattcggtgg cccgcgacga tctctccttg gggatcgacg
    10681 atcgcaacac cgatctgagc tgctttttcg cgctctagct tctcaagatc ggtcttgaag
    10741 gttaattttt ccgacgcata tgcagagtga gcgatccctg ccagtaatag tgcgtgaatg
    10801 aagcgcatgg tgatcccttg agatggaaat tatctttgta cgcccgtcta acgtttgaca
    10861 tgaggggcgg ccaagggcgc cagcccttgg acgtccccct cgatggaagg gttaggcaac
    10921 actgcgtgtt cgctcgaatg cctggcgtgt ttgaaccatg tacacggctg gaccatctgg
    10981 ggtggttacg gtaccttgcc tctcaaaccc cgctttctcg tagcatcgga tcgctcgcaa
    11041 gttgctcggc gacgggtccg tttggatctt ggtgacctcg ggatcattga acagcaactc
    11101 aaccagagct cgaaccagct tggttcccaa gcctttgccc agttgtgatg cattcgccag
    11161 tgactggtct attccgcgta ctcctggatc ggtttcttct tcccaccatc cgtccccgct
    11221 tccaagagca acgtacgact gggcataccc aatcggctct ccattcagca ttgcaatgta
    11281 tggagtgacg gactcttgcg ctaaaacgct tggcaagtac tgttcctgta cgtcagcaag
    11341 tgtcgggcgt gcttcttctc cgccccacca ctcgacgata tgagatcgat ttagccactc
    11401 atagagcatc gcaaggtcat gctcagtcat gaggcgcagt gtgacggaat cgttgctgtt
    11461 ggtcacgatg ctgtactttg tgatgcctaa cgttgaaatc agcggcgcca aaggcgtccg
    11521 ctggattgac tagttgggcg gcaacgcgtc gatagagtgg aggattgccc gcacgattgc
    11581 ctctcttttg aaaagatcat ccattctgtt tggcgtatcg atattcaatg cgaagaatac
    11641 ggcgccggta ggccactcaa cccatcccac ccaccagcca aacctacctt cccagccagt
    11701 ctttgcgcgc agaatccagt tgcgcccggc ttccgtaatc atgagatctt tgaccaagcg
    11761 ctgatgctct acccgaaatg gcaattcatt tcgatagagt tttctgagaa acgaaatctg
    11821 ttcgtacgca gaaatttcta gagtgccatc tatccaataa tcgcctttgc ttgtcgaagg
    11881 gtcggcgttg ccataaccta tttgctttaa atagcgtttg gctttttcct caccgatctc
    11941 tcttgcaaat agctcataaa cccagagcgt agagtttcgc atagctgatc gcaagttttg
    12001 gtcttgattg tgtcctgcaa agcttcgttt aataccgtcc catcggaaaa cctggaactc
    12061 atcacgaaca gcacctacat caagtgcaaa aagtgtgtgt ggaatcttga atgttgatgc
    12121 aggcgagtaa cgttttgtcg cccgtgcttg atcaaaaacc gataggatgc gatctgcttg
    12181 acgttcgtct gccacaacta tcgtgccttt ggcttggtaa tcgtcgaaga acttcttcca
    12241 gt
//
