LOCUS       CP073308                4096 bp    DNA     circular BCT 28-APR-2023
DEFINITION  Klebsiella michiganensis strain CZ598 plasmid pCZ598_Col, complete
            sequence.
ACCESSION   CP073308
VERSION     CP073308.1
DBLINK      BioProject: PRJNA722384
            BioSample: SAMN18753391
KEYWORDS    .
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Finianos,M., Kraftova,L., Papagiannitsis,C.C., Adamkova,V.,
            Hrabak,J. and Bitar,I.
  TITLE     Genomic characterisation of three GES-producing Enterobacterales
            isolated in the Czech Republic
  JOURNAL   J Glob Antimicrob Resist 29, 116-119 (2022)
   PUBMED   35231657
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 560X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2021 13:51:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,187
            CDSs (total)                      :: 6,072
            Genes (coding)                    :: 5,925
            CDSs (with protein)               :: 5,925
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 49 of 147
            Pseudo Genes (incomplete)         :: 101 of 147
            Pseudo Genes (internal stop)      :: 24 of 147
            Pseudo Genes (multiple problems)  :: 18 of 147
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="CZ598"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="pCZ598_Col"
                     /geo_loc_name="Czech Republic: Pilsen"
                     /lat_lon="49.73 N 13.37 E"
                     /collection_date="2019-12-02"
                     /collected_by="FNP"
     gene            join(3893..4096,1..6)
                     /locus_tag="KDU73_30825"
     CDS             join(3893..4096,1..6)
                     /locus_tag="KDU73_30825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QUG49848.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(536..763)
                     /locus_tag="KDU73_30830"
     CDS             complement(536..763)
                     /locus_tag="KDU73_30830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QUG49849.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(785..1018)
                     /locus_tag="KDU73_30835"
     CDS             complement(785..1018)
                     /locus_tag="KDU73_30835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="QUG49850.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1100..1369
                     /locus_tag="KDU73_30840"
     CDS             1100..1369
                     /locus_tag="KDU73_30840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUG49851.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            2403..2726
                     /locus_tag="KDU73_30845"
     CDS             2403..2726
                     /locus_tag="KDU73_30845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QUG49852.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2665..3429
                     /locus_tag="KDU73_30850"
                     /pseudo=""
     CDS             2665..3429
                     /locus_tag="KDU73_30850"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3404..3889
                     /locus_tag="KDU73_30855"
     CDS             3404..3889
                     /locus_tag="KDU73_30855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QUG49847.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          2217..2295
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          complement(1653..2141)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          2717..4095
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.913"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcgtaaggct gaacgacatc gaggcacacc gggagcagga gcgcgcagcg cagcaccaga
       61 aagcgctgga gctggaacga tcgcagcgtc agcaggagta tgacggtccg tcgctgtgat
      121 aaccggataa aacggggctt tcattgagga tctgcgccga aacccggtgc cattcacccg
      181 gctgcatggc tatgcagtcg ggtgagattt atcaggcgca gaatgcacgg tttgcggggt
      241 ggtttactgt cggttttgca ggactgacgc cgcattcgcg ctgaacccgt ccggagaggt
      301 gcgggcaagg gatgttatgg taaaagcatg atgcctggcg ggtacggaaa aattgtggcg
      361 accgagcctg cagacgcaaa aatccggccc atacttattc gtctccttca tcaggaatgc
      421 agtactatcc agccactgca ggtatttggc ccccagcggg ggcttttttt gcctgttgtt
      481 ggggcagata aagaaaaggg ccgcattagc ggcccttttc ggagagagcg atgcctcaat
      541 gcggaatttc agagacttct ttaacgtcgg ctttattgat ctgctgtttg acaccatttg
      601 cgtctttata gcccagcagg cctgagtctg attctgtagg cttgccgtcg ctgacaattg
      661 tacgcccgtc gttggtgtgg atggcatagc ttgtgcgcgt gcagccggtt agggctgaaa
      721 gcgcaactgc cgctgcgaac actgaaacaa aaatcttttt caaagccagc tccttttatc
      781 cgtatcagtt gtctagttca ctcatgttgg taattttgtc acggttgatc tgttcggttc
      841 ggcctgtatg ggcatctttg taggatacta atcccgtatc actgtcaact ttgggtttgc
      901 cgtctgtaac gatagtgcga tcatctgtgg tttttatttc ttggtttgaa gaacatccag
      961 cagcggcaaa ggtcaccaac gcgaccatca tggccgccaa cagcgttttt ttattcataa
     1021 taggttccct gcttaaaggt aaagtggata tggtcactaa ccatctaagt ataggtcgtt
     1081 ttttcatgcg aaaagaggat tgaatggaca agaatttcga ttattgctga cagagcatgg
     1141 agaaaaagag gctgttttac tgttcggaaa gaaatctttt gttgccgtgc cagatacgca
     1201 gaacctttat ggtgggtgtt attaccttta tttagaggat gctcacaaga acgagctttt
     1261 aattgagtta aacgaaagtg atttataccc cggtggcgca atagaacttc aacacaacgg
     1321 cggtcggaag attcattgcg agatactaaa agtaaggtgg agtcgttaac tcaagtaatg
     1381 tttcctaagt atgttgatag gcaaacatag tggtagttag tctctctgag taaatgatct
     1441 tcttgtgatc ctgtcgttac agtcgtaatc tcttgctctg aaaacgaaaa aaccacctgg
     1501 ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccagg taactgggta
     1561 tacgagaccg cagaaaccaa aactgttctt tcagtgtagc ctctctcagg cccatacttc
     1621 aagacctctt aatttgtctc tcgtatacct ccagttacta atggctgctg ccagtagcga
     1681 tttttcgtgt ctttccgggt tggactcaag acgatagtta ccggataagg cgcagcagtc
     1741 ggactgaacg gggggttcgt gcatacagtc aatcctggag cgaactgcct tcgcggaact
     1801 gagtgtcagg cgtggaatga gaaaccgcgg ccataacagc ggaatgacac cggtaaaccg
     1861 aaaggcagga atgcggggga gcacgaggga gccatcaggg ggaaacgcct ggtatcttta
     1921 agccgcatcg ggtttcgcca ccactgattt gagcgtcaga ttctgtgatg cttgtcaggg
     1981 gggcggagcc tatggaaaac cggcagcggc gcatcctttc ttcccctcct gcattcacgt
     2041 atttccccgc cacgtcttta tccgtgagtc cgtaccgctc gccgcagccg aacgaccgag
     2101 cgtagcgagt cagtgagcga ggaagcggaa tatccaccgg gacaggcatc agcctcttac
     2161 gcacctgagc ctcatctcac agttttcatg actgactatt tcatcccgat tcccgtcgtg
     2221 ccaccatagt aagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc
     2281 ccgaaacccg ctaaatgcca ctgacgcgcc tgcggcttgc ccaacaccgc gccgaccggg
     2341 aaaagatttc ccgcccgtcc cggcgttatc aggaggccgg attggcagac aaacgcagca
     2401 aaatgctcac gatgtgggta acagaagatg agcaccgccg tctgctggaa cgctgcgacg
     2461 gtaaacagct cgcggcctgg atgcggcaga cgtgcctgga cgagaagccc gcccgggccg
     2521 gcaaacttcc ctcgatctcg ccggcgctgc ttcgtcagct tgccggcatg gggaacaacc
     2581 tcaaccagat tgcccgccag gttaacgccg gtggcagtag cggtctcgac cgcgtgcagg
     2641 tcgtcgccgc gctgatggcc atcgatgccg gactcgagcg gctgcggcat gccgtactgg
     2701 aaaagggggc tgacgatgat cgttaagttt catccccggg gacgcggagg cggtgccggc
     2761 ccggttgatt atctgctggg gaaagaccgc cagcgcgacg gtgccagcgt tcttcagggg
     2821 aagccggagg aagtccggga gcttatcgac gcctcgccct acgccaaaaa gtacacctcc
     2881 ggggtgctgt cctttgccga acaggactta ccgcccgggc agcgtgaaac gctgatggcg
     2941 agcttcgagc gggttctgat gcccggactc gataaagacc agtacagcgt gctgtgggtt
     3001 gagcaccggg acaaggggcg gctggagctg aacttcctga tcccgaacac ggagctgctg
     3061 accggcaaac ggctccagcc atactatgac cgcgccgacc gtcctcgcat tgatgcctgg
     3121 cagaccatcg tgaacggcag tctggggctg cacgacccga acgcgccgga aaaccggcgg
     3181 gcactggtca cgccgtccgc gctgccggaa acgaagcagg aagccgctca ggcgattacg
     3241 cggggtttac tggccttagc ctcgtccggg gagctgaaaa cgcgtcagga cgtcactgag
     3301 gcgctggaaa gcgcaggttt tgaggtggtt cgcaccacaa aaagcagcat cagcattgcc
     3361 gacccggacg gggggcgaaa catccgactg aagggagcca tctatgaaca gtcttttaac
     3421 gctggcgaag gacttagagc agaaatcgaa agtgcagcag cagactaccg gcgagatgct
     3481 gaaagccgca ttcagcgagc acgagaagtc tgtcagagcg gaactgaacg aaagcgagaa
     3541 gagaatcagc gccgccatcc acgaccacga ccggatgctg tcctcagcca tgagccagcg
     3601 tacgaaaggg atgctgcgca tggtcagcca gacgtggctg accatcgtcc tggtctccgt
     3661 gctgctgata gcctcaagtg cgggcattct atggtggcag gggcagcaga tactcgacaa
     3721 ttacacgacc atccgggagc agaagagcac gcaggccatg ctgtccgaga ggaacagcgg
     3781 cgtgcagctc acgacctgcg gagaggaacg acgccgctgc gtgagggtga acccggacgc
     3841 gggacggttc ggagaggatt cgagctggat gatactggcg gggaaatagc acatgacgga
     3901 actggaaaaa cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct
     3961 ggacgagtgg gagagcgcct tcgcggaatg gcggacgatg tgtggtctta tgcaacggga
     4021 gaacgcagcg ctgagcgagc gcgtcacgga cttgagcacg caggtgctga gtttaagcga
     4081 gcagctgcgc cggttg
//
