LOCUS       CP073306                3223 bp    DNA     circular BCT 28-APR-2023
DEFINITION  Klebsiella michiganensis strain CZ598 plasmid pCZ598_1, complete
            sequence.
ACCESSION   CP073306
VERSION     CP073306.1
DBLINK      BioProject: PRJNA722384
            BioSample: SAMN18753391
KEYWORDS    .
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Finianos,M., Kraftova,L., Papagiannitsis,C.C., Adamkova,V.,
            Hrabak,J. and Bitar,I.
  TITLE     Genomic characterisation of three GES-producing Enterobacterales
            isolated in the Czech Republic
  JOURNAL   J Glob Antimicrob Resist 29, 116-119 (2022)
   PUBMED   35231657
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 560X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/21/2021 13:51:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,187
            CDSs (total)                      :: 6,072
            Genes (coding)                    :: 5,925
            CDSs (with protein)               :: 5,925
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 49 of 147
            Pseudo Genes (incomplete)         :: 101 of 147
            Pseudo Genes (internal stop)      :: 24 of 147
            Pseudo Genes (multiple problems)  :: 18 of 147
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="CZ598"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="pCZ598_1"
                     /geo_loc_name="Czech Republic: Pilsen"
                     /lat_lon="49.73 N 13.37 E"
                     /collection_date="2019-12-02"
                     /collected_by="FNP"
     gene            join(3096..3223,1..358)
                     /locus_tag="KDU73_30790"
     CDS             join(3096..3223,1..358)
                     /locus_tag="KDU73_30790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QUG49841.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            362..577
                     /locus_tag="KDU73_30795"
     CDS             362..577
                     /locus_tag="KDU73_30795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QUG49842.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            745..1077
                     /locus_tag="KDU73_30800"
     CDS             745..1077
                     /locus_tag="KDU73_30800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="QUG49843.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(1286..1456)
                     /locus_tag="KDU73_30805"
     CDS             complement(1286..1456)
                     /locus_tag="KDU73_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QUG49844.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNWVHGAEIV
                     ENAYRVLEVKA"
                     /name="replication initiation protein"
     gene            2095..2418
                     /locus_tag="KDU73_30810"
     CDS             2095..2418
                     /locus_tag="KDU73_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QUG49845.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2357..3121
                     /locus_tag="KDU73_30815"
                     /pseudo=""
     CDS             2357..3121
                     /locus_tag="KDU73_30815"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             746..1077
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          1808..1999
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1345..1825)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          2..675
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.784"
                     /coverage="100.0"
                     /evalue="9.66e-119"
     Typing          2409..3223
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.324"
                     /coverage="100.0"
                     /evalue="8.23e-180"
ORIGIN
        1 aagcgagaag agaatcagcg ccgccatcct cgaccacgac cggaagctgt cctcagccat
       61 gagccagcgc acgaaaggga tgctgcgcat ggtcagccag acgtggctga ccatcgttct
      121 ggtctccgcg ctgctgatag cgtcgagcgc gggcattctg tggtggcagg ggcagcagat
      181 actcgagaac tatacgacca tccgggagca gaagagcacg caggccatgc tgtcagagag
      241 gaacagcggc gtacagctct cgacctgcgg cgagcagaga cgccgctgcg tgagggtgaa
      301 cccggaagcg ggacagttcg gagaggactc gagctggatg atactggcgg ggaaatagca
      361 catgacggag ctggaaaaac agttgctgag cgcattagag cagctacagc aggactactc
      421 gaaaaggctg gacgagtggg agaacgcctt cgcggaatgg cggacgatgt ctggtcttat
      481 tcaacgggag aacgcggcgc tgaacgagcg cgtcacggtc ttgagcaggc aggtgcagag
      541 tttgagcgag cagctgcgcc ggttgtcgaa aggctgaacg cgatagaaac tcaccggcag
      601 caggagcggg cggtgcagca ccagaaagcg ctggagctgg aacgatcgca gtggcagcat
      661 catggtcctt cgctatgata agccgggttc tattaattag ttgttatgta aagatattta
      721 aatttttcaa catgaggtat ttttatgaat acatatattt tcctgggagg ggctattgtg
      781 gctgaagtca taggcaccac ctttatgaaa tattcagaag gatttacacg cctgtggccg
      841 tctgtaggaa ctgcaataag ttattgcgct gccttttatc tgctttccca aacattggaa
      901 aatataccta ccggtattgc ttacgcaatt tggtctggtg cgggaattgt actaataagc
      961 ctagctggtt ggttggttag cggtcaaaaa cttgattggc ccgcaattgc aggaatgttc
     1021 cttatttgcg ttggagtttt ggttattaat attttttcaa aatcttctgc gcattaactt
     1081 tttaccgaag catattggcc aaagcaaaag gatttccttg agatcctttc tttctgcgcg
     1141 taatcttttt ccctgtaaac aaaaaaacca ccgctaccaa cggtggttcg tttgccggaa
     1201 ttcagagcta ccaactcttt gttccgatgg taactggctt tcacggagcg cagataccta
     1261 aactgtcctt tcagtgtagc cgcactcagg ctttcacttc aagaactctg taggcattct
     1321 ctacaatctc tgctccgtga acccagttac caatggctac cgccactggc gatttgtcgt
     1381 gtctttccgg gttggactca agttgatagt taccggaacg ggcgtggtag tcggactgaa
     1441 cggggggttc gtgcatacag tccatcctgg agcgaactgc ctacccggaa ccgagtttca
     1501 ggcgtggaat gagaaaccgc ggccataaca gcggaatgac accggtaaac cgaaaggcag
     1561 gaatgcgggg gagcacgagg gagccaccag ggggaaacgc ctggtatctt taagccgcat
     1621 cgggtttcgc caccgctgat ttgagcgtca ttttcgtgat actcgtcagg ggggcggagc
     1681 ctatggaaaa acggcaactc cgcatccttt tcttggcctt tcctgcttcg agcggcattt
     1741 gcttatcccg taatctgtga gctggtaacg cccgccgcag tctcacggca ggagcgtagc
     1801 gaccgagtga gcgaggaggc gtcatttcat caccacctgc attttgcact tacgcccggc
     1861 agccgcattt ttcccctgtc acatgaagca ctttcataaa ttgccactcc gtcataacat
     1921 gtttagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc gccccgaaac
     1981 ccgctaaaac cactgccgcg cctgcggctt gtccaacacc gtgccgaccg ggaaaagatt
     2041 tcccgtccgt cccggcgtta tcaggaggcc ggattggcag acaaacgcag caaaatgctc
     2101 accatgtggg tgactgagga cgagcaccgg cgtctgctgg agcgctgcga gggtaagcaa
     2161 ctcgcggcct ggatgcggca gacgtgcctg gacgagaagc ccgcacgcgc cggcaaactt
     2221 ccgtcgatct cgccggcgct gcttcgtcag cttgccggca tggggaacaa ccttaaccag
     2281 attgcccgcc aggttaacgc cggtggtgga agcggacacg accgcgtgca gattgtcgcc
     2341 gcgctgatgg ccatcgatgc cggactcgag cggttgcggc atgccgtact ggaaaagggt
     2401 gctgatgatg atcgttaagt ttcatcctcg ggggcgcggc ggcggcggcg ggccggtcga
     2461 ttatctgctg ggtaaagatc gccagcgcga cggcgccagc gttctgcagg ggaagccgga
     2521 tgaagtccgg gagcttatcg acgcctcgcc ctacgccaaa aagtacactt ccggcgttct
     2581 gtcttttgcc gaacaggatt taccgcccgg ccagcgcgaa aagctgatgg cgagtttcga
     2641 gcgggttctg atgcccggac tcgataaaga ccagtacagc gtgctgtggg ttgagcaccg
     2701 ggacaagggg cggctggagc tgaacttcct gatcccgaac acggagcttc tgaccggcaa
     2761 gcggctacag ccgtattacg accgcgccga ccgtccgcgc atcgatgcct ggcagaccat
     2821 agtgaacggc aggctggggc tgcatgaccc gaacgcgccg gagaaccggc gggtgctggt
     2881 ctcgccttcc gcgctgccgg aagcgaagca ggaagccgcc caggcgatta cgagcggcct
     2941 gcttgccctt gcctcatccg gggagctgaa aacgcgtcag gacgtcactg aggcgctgga
     3001 aagcgcaggt tttgaggtgg tgcgcaccac aaaaagcagc atcagcattg ccgacccgga
     3061 cggggggcga aacatccgac tgaagggagc catctatgaa cagtctttta acgctggcga
     3121 aggacttaga gcagaaatcg aaagcgcagc agcagactac cggcgagatg ctgaaagccg
     3181 cattcagcga gcacgagaag tctgtcagag cggaactgag cga
//
