LOCUS       CP073295                5596 bp    DNA     circular BCT 26-APR-2021
DEFINITION  Klebsiella pneumoniae strain 135077 plasmid p3_135077, complete
            sequence.
ACCESSION   CP073295
VERSION     CP073295.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN18793521
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zhao,Q., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 135077
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zhao,Q., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2021) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/20/2021 11:29:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,740
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,479
            CDSs (with protein)               :: 5,479
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 51 of 140
            Pseudo Genes (incomplete)         :: 87 of 140
            Pseudo Genes (internal stop)      :: 20 of 140
            Pseudo Genes (multiple problems)  :: 18 of 140
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="135077"
                     /isolation_source="urine"
                     /db_xref="taxon:573"
                     /plasmid="p3_135077"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2020-06"
     gene            join(5494..5596,1..242)
                     /locus_tag="KC089_28630"
     CDS             join(5494..5596,1..242)
                     /locus_tag="KC089_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QUF98502.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            181..945
                     /locus_tag="KC089_28635"
                     /pseudo=""
     CDS             181..945
                     /locus_tag="KC089_28635"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            920..1429
                     /locus_tag="KC089_28640"
     CDS             920..1429
                     /locus_tag="KC089_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QUF98503.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1436..1648
                     /locus_tag="KC089_28645"
     CDS             1436..1648
                     /locus_tag="KC089_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QUF98504.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2315..2539
                     /locus_tag="KC089_28650"
     CDS             2315..2539
                     /locus_tag="KC089_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="QUF98505.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="TraY domain-containing protein"
     gene            2523..2789
                     /locus_tag="KC089_28655"
     CDS             2523..2789
                     /locus_tag="KC089_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QUF98506.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2859..3209)
                     /locus_tag="KC089_28660"
     CDS             complement(2859..3209)
                     /locus_tag="KC089_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUF98499.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3275..3820)
                     /locus_tag="KC089_28665"
     CDS             complement(3275..3820)
                     /locus_tag="KC089_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUF98500.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3846..4225)
                     /locus_tag="KC089_28670"
                     /pseudo=""
     CDS             complement(3846..4225)
                     /locus_tag="KC089_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5328..5504
                     /locus_tag="KC089_28675"
     CDS             5328..5504
                     /locus_tag="KC089_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUF98501.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          5163..5242
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(4580..4977)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          233..1764
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggaaacctg tctcgatacg cgcccggcgc ggtcgttgcg gctgccgtcc atcgatccgg
       61 ttttgctgcg ccagcttgcc gggatgggga acaacctcaa ccagatcgcc cggaaaatta
      121 acggcggcca gtggtcaggc gctgaccggg ttcaggtggt ggccgcgctg atggccattg
      181 atgccgaact cgagcggctg cggcatgccg tgctggaaaa gggggcagac gatgatcgtt
      241 aagtttcatc cgcgagggcg aggcggcggt gccgggccgg tggattatct gctggggaaa
      301 gaccgccagc gcgacggggc catcgtcctg cagggaaagc cggaggaggt ccgggagctt
      361 atcgacgcct cgccctacgc caaaaagtac acctccggcg tcctgtcttt tgctgaacag
      421 gatttaccgc ccggccagcg tgaaaagctg atggcgagct tcgagcgggt tctgatgccc
      481 ggactcgata aagaccagta cagcgtgctg tgggttgaac accgggacaa ggggcggctg
      541 gaactgaatt ttctggtacc gaacacggaa ctgctgaccg gcaggcgtct ccagccgtac
      601 tacgaccggg cagaccgtcc gcgcatcgat gcgtggcaga ccgtggtgaa cgggcgactg
      661 gggctgcacg acccgaacgc accggagaac cggcgcgcgc tggtgacgcc atcttcgtta
      721 cccaaaacga agcaggaagc cgcagaggct attacgcggg gcttactggc cctcgcctcg
      781 tcaggggagc ttaaaacgcg tcaggacgtc actgaggcgc tggaaagcgc aggttttgag
      841 gtcgtgcgca ccacaaagag cagcatcagc attgccgacc cggacggagg gcgaaacatc
      901 cgacttaagg gagccatcta tgaacagtct tttaacgctg gcgaaggact tagagcagaa
      961 atcgaaagcg cagcagcaga gtaccggcga aatgctgaaa gccgcattca gcgagcacga
     1021 gaagtctgtc agagcggaac tgagcgaaag cgggaagaga atcagcgccg ccatccgcgc
     1081 ccacgagcag ggtatgagcg cggccatgca gtcgaaccgc ctgagcgtga tgcgtatggt
     1141 cgggcggaca tggctgacca tcacgatggt gtcaggactg ctgttcgcca gcctgagcgg
     1201 ggtgttgtgg tatcagggga gcttgatagc gtcaaatctg gccgaaatcg accggcagaa
     1261 cgcagcgctg tcgaagctga acgcgaagac ctggggcgtg acgtacctgg aagacagcaa
     1321 cggacgtttt ctggtgctgc cgaaggggac ggccgtagac aggacgcaga gctggacggt
     1381 cgggaacggg cggtcgaagc agaacgcgct gagactggtg aaggagtaag tgccgatgac
     1441 cgagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag
     1501 gctggacgag tgggagagcg ccttcgggga atggcggaaa atgtcagggc ttatgcaacg
     1561 ggagaacgcg gcgctgagcg agcgcgtgac gcgcttgagt cagcaggtgg aacgcttgag
     1621 cgggcaactg cagcgtttga gccggtagtg cagcgtcatg agatggccgt ggccgcagaa
     1681 cgggcgcacg agcaacggca gcacgaaaag gaactgacgg aagcgcgttc ccggcagcga
     1741 agctatgacg gtccgtcgct gggctgaaat gcaggaaaac agggttctca tcgggcattt
     1801 acgctgaaac cggatgtcat tcacctggct gcatggctat gcagccaggt aaaatttatc
     1861 tgtcgcgaac ggggcggttt atcgggtggt ttgttgccgg ttttacctga ctgccacccc
     1921 gttcgcggcg aacccgtccg gggcggtgcg ggcaacgggt gttatgttta atcagcgtga
     1981 acggcagata caaaaaagcc ccggagggct tttaactact gacttatttt tttgaagatt
     2041 tgctgggttt caatttgtca gtatttcccg gcgttttttt attgtcacgc tggcgtttat
     2101 caggttcccc tgttgaatcg gcaattcgtt tcggacccgg cttaagaccc ataatcgtta
     2161 tcctttcaat agttaagagg aaagccctca agctagatat attgaccgcg ttacggatta
     2221 tcaagagggg ctaacaagat tattttttgt tcttttaggt tgattgtggt actgttgata
     2281 tacatgtata gcaaatggtg ttcgggaggt ttatatgctt gctatccggt tatctgatga
     2341 gattgagtcc cgtctggact cgctggcgaa gcaaaccggc agaacaaaga cgttttatgc
     2401 gcgggaagca atactggcgc atctggaaga cctggaggat tattatcttt cagcagaaac
     2461 tgctgcacgc gttcgccgtg gtgatgaagc agtgcattcg tctgaagacg tgaggaagtc
     2521 acttggtctg gacgattaac tattccgatc gggcgctcaa atcgttacgc aagatggaca
     2581 aacagaacgc acgacggatt gtggatttta tgagtttacg cattgcagtt gctgccgatc
     2641 ctcgccagtc agggaagccg ctcaaaggtg agctgggcga gttctggcgc tatcgggtgg
     2701 gagattatcg cgttctgtgt gagatccgag atgacgagct tgttatcctt gccgccacga
     2761 ttggacatcg ccgcgaagtt tacgactgaa gcccgctatg cgggcttttt tgatgccgac
     2821 aacaggtatt atggtaaata ctcgactaac tacgtgttct actgtttgtc ggttttagtt
     2881 aaccagcagg ttgtcttaac gataatgtag ccgttatttt taactaataa ggtcatattg
     2941 ttaacgtcag gcataataaa agacattatg gcctcagagt tatcatcaga agcagctctc
     3001 tgcattacta cttcgattcc cttttctttg aatctataag tatccagttg cctattaaac
     3061 ttagaaagac ctacaaccat acttagtttg ttatctttaa cagtaagaga tgcttcctga
     3121 gtaccattac atcggtagta agcggtgtaa ccgtcagcaa aagatcgacc ggggaatata
     3181 aatgctagga acgcaattaa ccaatacata gttgagaatc ttggtattac acatttcact
     3241 atcctctctt tcatttttcg ttcaccatac tttgttatct gatttttaat ttttctattt
     3301 tataaaccaa gtctctacca aaaaggcgaa aattatcctc aaataataat aattgtttaa
     3361 gaacatccat gaaagcagct gcttcaccct cagttatggg agctactcta tataacatat
     3421 aacctgagtt aagggtttct tttatacttt cttcaatgct cattataaat attagtggat
     3481 tatcattttt cttcccccat gggtttctaa ttaatccttc gatgtactca ggtcctttgc
     3541 gtcctattgc ctgcgcaata ttttttattt catctgcgtg agggctttgt tgtacagctt
     3601 tgtttatgcc tgcttgagtg attttcagag actctacacc gtgtatgttg tatgagataa
     3661 aatgtaataa attaagatgc aatgcaatta atccattttt gtttacatct gagtatttgg
     3721 ataaaaaacg taatgaagtg ttaactctcc cttgaagcct aacaattggg agtgctgcct
     3781 tgtatatttc cttctccgtt aaataccgtt taaaaaacat aattctccgc ctccgtatag
     3841 cgtgcctatt ttatttcgtc gtaaaactta gctctgaaat attcctttag aaccatcatg
     3901 tcattaataa ttgaatctat ctcatccatt ttttcttggt attcttttaa tggtgaaggg
     3961 agcggtaact caatatttga aaagtgattg gcgagatagg gatcaaggtt tctcatccat
     4021 tcaggctttt cgccaatgcg ttcttcaact gaaagtgaac ctgataatct ctggttaata
     4081 agatcactta gactcatttt ggttttattc gaaagccgtt gtaaggattg ctttaatcct
     4141 atatctagca tgatatttgt tctttctctc attacccccc cttgctattg cgttttttgt
     4201 tgcgcacaaa gctaccattg cgcattaaga aacgcaataa aaaaggaggg agggagtggt
     4261 ttgtacatga tgtgcagtgc gctgagggca tcagtggcgg gtgtttttcc gtgggatgga
     4321 tggagctccg gggataacag aactgacgat catttaaagg atcttcttga gatccttttt
     4381 ttctgcgcgt aatctcttgc actgtaaacg aaaaaaccgc ctggggaggc ggtttgatcg
     4441 aaggttaagt cagttgggga actgcttaac cgggtaactg ggtatacgag accgcagata
     4501 ccaaaactgt tctttcagtg tagccgcagt taggcaccca cttcaagacc tctcaatatc
     4561 tctctcgtat atatccagtt accaatggct gctgccagtg gcgtttttgc gtgtccgtcc
     4621 gggttggact caagatgata gttaccggaa agggcgcagc agtcgggctg aacggggggt
     4681 tcgtgcatac agcccagctt ggagcgaacg acctaaaccg agccgagata ccagcagcgt
     4741 gagctatgag aaagcgccac gcttcccgaa agggaaaaag gcggaacagg tatccggtaa
     4801 acggcagggt cggaacagga gagcgcacga gggagccgcc ggagagaaat caccggcatc
     4861 tttaagtcct gtcgggtttc gtcctcctct gacttgagcg tccatttctg tgatgctcgt
     4921 caggggaggc ggagcctatg gaaaaacggc aacgccgcat ccttttctcc ggccctttct
     4981 gatttgagcg cctttgcttt atcccgttat ccgtgagtcg gtcacgcccg ccgcagccga
     5041 acggcaggag cgcagctacc gagtgagcga ggaggcgtca tttcatctgt ccgtgcatgg
     5101 tattgcggac gctcctgagc cacatatcac acctgccaca tgaggcactt tcatgaatcg
     5161 tgctctcatg ccaacatagt aagccagtat acacagcgta aacgctgtga ctggttcagg
     5221 gctgcgcccc gaaacccgct aacaccactg acgcgctggc gcttgtccaa cgccggagcg
     5281 acccggaaaa cttttccgtg ccgtccggcg tttgcgaagg agggataatg cagaagccga
     5341 agaagaagcc gctcgtcgta aaaaaaagat cctggcatcc ggcagtgatg gcaaatctga
     5401 tgcgtcaggc acaggaggaa gattaccgcc gggagcagat ggcagagctt gagcgttcag
     5461 cccggtcaca ttttgcggat atcggaggag aggatggcgg ttaagcgtac ccgtttttta
     5521 ggtatccggg tgaccgatgg ggagtaccag cagctgctgg agcgctgcaa tggaaggcaa
     5581 ctggcggtgt ggatgc
//
