LOCUS       CP073294               10060 bp    DNA     circular BCT 26-APR-2021
DEFINITION  Klebsiella pneumoniae strain 135077 plasmid p2_135077, complete
            sequence.
ACCESSION   CP073294
VERSION     CP073294.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN18793521
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Zhao,Q., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 135077
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Zhao,Q., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2021) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/20/2021 11:29:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,740
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,479
            CDSs (with protein)               :: 5,479
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 51 of 140
            Pseudo Genes (incomplete)         :: 87 of 140
            Pseudo Genes (internal stop)      :: 20 of 140
            Pseudo Genes (multiple problems)  :: 18 of 140
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="135077"
                     /isolation_source="urine"
                     /db_xref="taxon:573"
                     /plasmid="p2_135077"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2020-06"
     gene            complement(join(8695..10060,1..599))
                     /locus_tag="KC089_28565"
     CDS             complement(join(8695..10060,1..599))
                     /locus_tag="KC089_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="QUF98494.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(2083..2232)
                     /locus_tag="KC089_28570"
     CDS             complement(2083..2232)
                     /locus_tag="KC089_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QUF98495.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(2317..2574)
                     /locus_tag="KC089_28575"
     CDS             complement(2317..2574)
                     /locus_tag="KC089_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QUF98496.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="cloacin"
     gene            complement(2584..4269)
                     /locus_tag="KC089_28580"
     CDS             complement(2584..4269)
                     /locus_tag="KC089_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="QUF98497.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            4596..4841
                     /locus_tag="KC089_28585"
     CDS             4596..4841
                     /locus_tag="KC089_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUF98498.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5115..5486
                     /gene="tolA"
                     /locus_tag="KC089_28590"
     CDS             5115..5486
                     /gene="tolA"
                     /locus_tag="KC089_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="QUF98487.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            5483..5689
                     /locus_tag="KC089_28595"
                     /pseudo=""
     CDS             5483..5689
                     /locus_tag="KC089_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /name="TonB family protein"
     gene            6023..6301
                     /locus_tag="KC089_28600"
     CDS             6023..6301
                     /locus_tag="KC089_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="QUF98488.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6289..6603
                     /locus_tag="KC089_28605"
     CDS             6289..6603
                     /locus_tag="KC089_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="QUF98489.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6767..7195
                     /locus_tag="KC089_28610"
     CDS             6767..7195
                     /locus_tag="KC089_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUF98490.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7221..7400
                     /locus_tag="KC089_28615"
     CDS             7221..7400
                     /locus_tag="KC089_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QUF98491.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7427..7957)
                     /locus_tag="KC089_28620"
     CDS             complement(7427..7957)
                     /locus_tag="KC089_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QUF98492.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7964..8695)
                     /locus_tag="KC089_28625"
     CDS             complement(7964..8695)
                     /locus_tag="KC089_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="QUF98493.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     BGC             1..9269
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1402..1980
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7968..8695)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg cctcgtcagc ggccgcaaaa
       61 tgaccatcaa gcagcaggaa cgccagcagc cgcgccagaa ggacgccggc gacaagccct
      121 ccggcacacc aggctatccc gaacaggtta gccggctgca gcaggtaata atctgcgttc
      181 gcatagaggt ccgggcgcag gacggtttct ccccagctct cgataaacac ggcgacaacc
      241 agccccccca gaagggccag accggcagcg ccggtcagcc agtacgcgta gttccagaca
      301 tgtcgccgtg ccctgaccat ccgcccgggt gaatccagcg aggccagcac cgaaaatggc
      361 acagtccggt tattatcgcg cttatagcga tcggcatact cccgccgcgc cttatggctc
      421 agccccacaa aacgcattcg gccggcccag agttcagcca gaccgaaaaa caggccgata
      481 accagaaaaa aaccggccag actgtaatac tcccggtaca gccagctgac ccacgggtaa
      541 atctgccggg ccagctccgg gatatgccac tgccaggcaa gcgaatccgt attaaacatg
      601 caatctctct ccttcagacg acgggtttac cggcgtgatg cgcagccccc gctgcacaag
      661 ccgcggacat gccccaaggc atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct
      721 ttcaccgcgg aggccagcct ctttccgccc catgcggaaa aggcgcagcc ccggaggaac
      781 aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc ccagtgcggt cttttccggt
      841 gttccggccc ccgtcagagt tcgccatagc gagcggctga ccacccgcaa aatcaggagc
      901 agctttgctg cttcgtcagg tgcgtccagc accgtggatt tttgtgggtg gtcaggcgtg
      961 agcctgacgg gggccgggtg aatttctggc gtggccggcg tccagccgga cagagccaga
     1021 aagaggggaa aagaccgtgc gtcggtggcg tccagccagc gtaagcaagg tcttttcccc
     1081 tgaagtcagc cacgccccag cggggatgac ttgcatttct ccgcaaggag aaaaaagcaa
     1141 cagagatagg ttaacgtaaa atgttcagcg aaaaaccgac atggttcacc tatcctgata
     1201 attgatcgtc agggaataga aagacttaat caggggacaa tatcccacat cagcgcgtcc
     1261 ggtgaccgtc ttgcaggtgc cggggaaatt gcgcctcctc gctcactcgg tcgctacgct
     1321 ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg
     1381 gataagcagg ggactaacat gtgaggcaaa acggcagggc cgcgccggtg gcgtttttcc
     1441 ataggctccg ccctcctgcc agagttcaca taaacagacg ctttttcggt gcatctgtgg
     1501 gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga
     1561 tccccaccgt ttccggtagg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt
     1621 accggatacc tgtccgccgt tctcccttgc gggaagtgtg gcgctttctc atagctcaca
     1681 cactggtatc tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc
     1741 cccgttcagc ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggga
     1801 aagcacgcaa aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt
     1861 ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac
     1921 agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga
     1981 cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta
     2041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc
     2101 cgtcagttca gaggaggacg atggtgccac cgttccgccc tgaacatccc ggatataatt
     2161 tgcctgacat gcggccagaa gaaagccagc aacaatcaat ataaaaagaa aaatagtctt
     2221 tacctttttc atatactttc cctgtattta aagactccgg cattcatccc tgaacaccgg
     2281 aagtcagtat ttcaaatcat ccttgataat tttttgttac cagttaccgt cccgataatc
     2341 aaaagctata taacaattat ccacatcagg ttttatttga tttttaaaaa atggctgtag
     2401 taaaggaacc cattctttct tcaaatcaaa ctcaccatta ttaatattat cctttaaagg
     2461 cattcccaag ctttcaatga cagaaccatc gtcaccaaag tctttagaat actcttcccc
     2521 tttaaactct tcagttttct tatcaaacca tgtcaaacgt aatttaagcc ccatatttac
     2581 ctctcaaaga tattttttaa tgttccgttt tggatctggc cctttaacct gcttacccgt
     2641 tttgggatca aatgcgccta ggtgttcgcc atcactggca cgataccctt caagctcacc
     2701 gtgctgggaa tcccattcat aaattttacg ttttttatcg ccataccatc gggcccgctt
     2761 accaccacca ccttgtttag gtgttttagg atcacctcgc tttaactctc caagtccctt
     2821 tatgtcttct gttttaggta ctggatgata atcatggccg tagtctttag tccccttgcg
     2881 aggcttgttc ttttcctcat ttaacttatt ttctgcatcc ttcgcttttt tctccttttg
     2941 tttccggctc tccatcgccc cggtaagtgc agccttatct ctggataagg attcattcat
     3001 tgcgtttaaa gatgcctgag ccgcttttaa tttctcattc tgattctgaa tatccttatc
     3061 aagatagcga acctgatcct gccacccgga gccatattca tgaggcgggt tataagtcac
     3121 aaaatcatct ttatttgcct ctgcctcttt taccttttta tcggcttcct gaacagcagc
     3181 ccttgcaccg gggagcgcct tctctgtcga tgcaatgcgg ctgtttaagg tcgcaatatt
     3241 tttatcttcg gcatccagct cagctttcgc tttatcatag tctcttttca gcccctcttc
     3301 cggatgagcg gcgtcccatg cctgctggcg acgcttttct tcctcctgac gctgttttac
     3361 ctgtgccggg gtaagaacat ctgtcaccga cacataaacc ggcttctgtc cggtctcttt
     3421 cgggaaacga ataacggcct cacgggagtt accaccggct gtaaaaccag ccggacgtga
     3481 atcacctttt tcagcaacaa tgccttttgg cggggctttc gctgtcggaa cacctttcgg
     3541 tacgctcacc tgcagagacg ggagacccgg aatcgacaca ctgaataccc ccggacgttt
     3601 tgtcggtttc gcatccacca cagggacact cattggccga cctgcgacaa ccgcaatatg
     3661 ctgccgctca tccttcacca catccacaac ccgttgtctg acacgaaccg tcgcctggtc
     3721 cagtggtaaa gtacttgccg gagtctccgt caccgcatcg gccggtaatg acgtcataat
     3781 ttttgacatc attttcggat catcttttgc aatctcagaa ggcagcacac cgtagatcgc
     3841 aatcccccac agaccaaact taaacggccc cttcagggca gccagcacat cagcaacggc
     3901 ggccgacaac gcatcgccgg aaactgataa agccagtcct tcagcccccg gagtagccag
     3961 ggcaggcaga ccgaaggcca tcgcagtggc cgaagactgt ccgccaccag agctaccgct
     4021 atgattgccg ctgttaccac cattaccacc gggtttactc ccgccgttac caaactcccc
     4081 cggattatag ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc ccggcccgct
     4141 gtttggatcc gtaccaccac ctgatgaagg gccaccttta ccagacgtac cgttcacatt
     4201 cccactggcc tgaccaccat tatgtcccag acctgaatta cccggacctc gaccgtctcc
     4261 accactcata ccgttttcct ctttgacatt taaaacaata aattaaagcc aacatactgt
     4321 acatatacac agtataatta ttaccttact cctcccgttt tcaggtgtca atatatgaga
     4381 ttagttctca tcttcaggca caaaaaagcg cccggagcca cctgtaagca gatcgcgtct
     4441 tttcccgaaa atttctcatg gcaacaaatt ttattattta agacattcat cacacttaca
     4501 tttcagatgg tatagacaca tctgaaagct aactggcatg aggagaggac ctgcggttaa
     4561 actgttcggg aaacctgaga cgcccggagg ccactatgac gcacctgaaa ctgactcccg
     4621 ctgaagaaaa gcgcctggcg gacgcctttg tcgctcaggc actggccatg ggcctgaaag
     4681 ggaacaaaac gctgccaaag gcggtaaaac aacaggtgcg ggaaagggtg cttcgtgcca
     4741 ttcaggacga acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa tacgaggcta
     4801 cggaaaccac cttcacctgg cagccacagc gccgccggta aggggtattt accataacgt
     4861 cgcattgccc gcaccgctca gacggtctga gggcgtttcc ggcggctacg ggataaaaca
     4921 ggcaacaaac cacccggaaa accgccggaa acgcgcccgg taaattgtaa cccgctgcat
     4981 gcctatgcac gcaactgcat ggcgggccgt ttggtgtaaa aacgatacga taagccgtgt
     5041 ttatgtaaat gaacgtggcc gtaaagagtg acaaatatac atgtaacatt ttttactgac
     5101 aggtcatcaa cccgatgcgt ttaataccct ttatcctgct ggcattgtgc agccccttac
     5161 tcctgacggc ctgtaagcat tccaccaccc agccgccgaa aatttatact ctggataagt
     5221 catctaaaat cagtagctac gctgcggcca tcaagaccgc tgttgaaatg cagatgtttg
     5281 aaccagaggc ctatgcggga aaaatgtgca ccgtgcggat atccatacaa cgtgatggca
     5341 ctctgaacag cgccattgct gaaggaggag atccgaaact ctgtaaagca gcaatatcag
     5401 ccgttaccca cgccaaaatt ccgccggcac cggatgaaaa tacctggcaa atattcaaaa
     5461 acgcccctct ggatttcaga ccatgaaagc ttttactctg acagtgctgg cggccatgtt
     5521 cctcactccg gtacatgcga taccactaaa acccgtaacc acactgcagc caacctaccc
     5581 ggtacaggcc gcagcaaaac gcatcgaagg tcaggtgacc gccacgtttg atattctgga
     5641 cgatggacga gtggacaacg tgtcgcttac cggctgtcac cttttttttc gggaaacgct
     5701 gacggcaatg aggcactggc tatatcccgc agggcacccg gcaatgcgac aggttataaa
     5761 ttttgaattc agtctgaaaa atgacggctt acaggatccg cgaccagtca ccacccaaaa
     5821 gctcaatgat gatggttgcc gccagtaaca ggtcctttaa cccgcaggct gcaatagtcc
     5881 tggatgatga gtgagcctgt agcagccctg attagtggaa gcgaagctga aaaatgggca
     5941 gcaaaagccg tatttctggc aaatagcgcc gccacggatt aaagtagtca tgtgtaatac
     6001 atatgactac aaaggggctt ttatgtcatc agcaacatcc atcaggctcg atgaagaact
     6061 taaggaccgg ctgaaaacgc tggccgatga ccggcaccgc tcagcgcacg cgctaatgct
     6121 ggaggcaatc accgaataca tcgaccggga agagaagcgc agccagtacc tgcgggacgg
     6181 acaggccgca tggcagcact atcaggaaac gggtctgcac cttactgcgg aagaagcgga
     6241 agcctggata agtacctggg gaactgagaa cgagcaggat gctccgccat gccacaggta
     6301 acgatttccg cgctggcaca gcgcgattta caacgccttc aggactttct caaaaccaaa
     6361 aatcggctgg cagccagaaa aggaggtgag gtgatcgtcc gggctatcca gcaactgaaa
     6421 acattgccag acattggccg cccggtgccg tttctgccgc tggaatataa ggaactggtg
     6481 atcgggtttg gcgacagtgg ctatgtgatg ctctaccgcc acgaccggga aatggaccag
     6541 attgtgatcg tcaccgtcag gcatcagaaa gaatccgggt atccgggggc agacagcctc
     6601 taataaccga tgacgggggc gcaattcatt gcgtggccgt cgccggtttg acaagcgcag
     6661 cgcgtcagaa aaccacatcc tgaacggaaa aacgggtaaa attcagcgat acatttaccg
     6721 tgagaaatta cacacctttc acacccgata aaatcagggg tatacagtgg cgaaaacatt
     6781 gctcaacctt tcacaggcag cacaagccgc gggcattacc agaagaacgc tatacaatca
     6841 tgtaaaacag ggaaaggtta ccgtgtcacg tgacgggaaa aacaaccctg tcgtggatgt
     6901 gtcagaactg atccgtgtat acggaaatgt gaatatccct gagaaacaaa ttcccggcat
     6961 ttcacaccgt gaaaatacac aaaaaaattt cccacaggaa cagcttctgg caatgcaaaa
     7021 ggaactggcc gatctccggc aggctgtcac cctgatgctg gaagataaaa catcccggga
     7081 agaagaacgc cgccagcacg atgatgagcg ccggaaactc caggccgaag tggacagatt
     7141 gacaacagaa ctgacacaga agaaaaaacg cttctggtcc ggctggttta gctgagaact
     7201 taccgaaggg aatggagaac atgacgaagc aggaaaaaac tgccctcaat atggctaaat
     7261 tcattaagga tcagtcgctg ctgttgctgg aaaagctgaa cgagctggac atggataccc
     7321 cggccgacat gtgcgaacgg cttcacgagg atgcggaaac actctggcag gtgatgcggg
     7381 ataagctcgg ggaagaataa cagcaccggc catgccggtg ctggcgttat tttttgccga
     7441 acggccacca ccggcgggcg ggtttgtcct ccggcagttc aggcaccggc acgttaaaat
     7501 agcggctgtt atattcccgc atcagctccg ctttcagctt ctcgttcacc gcgctttccc
     7561 cgtgccgctc gctgaccacc tcaatcatcg cgttaatctg ctccggtgag cacttcccgg
     7621 catcaaccac ctgcacaccg ttctcgatgg cgaaccgctc cacctttcga tagaggtaat
     7681 cacacagctt cattcctgtg ggtttgtccc gttcccattg cggattttca ccggtgaaaa
     7741 agtacgtggc tttttcgtcg ctgttgagcc gctcgtcata catcccgatg gatttcatca
     7801 tcgccagatg ctgggcccgg tcgacaaaac agaaaacata cgccccggtc tgccaggcga
     7861 gcaccaccat gtcttccaga tgctcgctgg ttggcataat gcagcgctcg ccgtctttgt
     7921 cttccacgtc ctcaataaat accatgtaca gcgccataat ccctcactcc gttgtcataa
     7981 atttaaagca cgcgaacagc ctctcccggt ccagctccac cgttttgcgg gcctcgccgg
     8041 tctgtggatt cacgatttca tccaccgcca gaatcgcccg ctgtacccgg acaagcgtcg
     8101 gccggtcagg gcagatgtac cagatttcat cgatgccgtt cgcccggcgg ttgaacagat
     8161 acccggacac cacttcctga taccgcctgc ggctcttgac ggtgcgctct acctcaaatg
     8221 ccaccgttct gccgtccagc tccgccagcg cgtccggcac tttcatccct ttcagggcca
     8281 tacggtgcag gtaccgccag ccggtggccc cccggccctc catggagagg cgcagggact
     8341 gcacggccag ccggtgcggc agctgcgccg ccgacaccct tgacggctga aacggaataa
     8401 cgtccgtcag cggctcgtcc tcgtcgaacg agaacgccac cccgtgcggc gtcagccccc
     8461 acacgctgac ctgacggccg tcaaccgggt atttcgcccg gaccagcagc cctttcgcca
     8521 ccagactgtc caggaagcgg taaaccggct gtaccgccgc aatccccagc cgtcgcgcaa
     8581 ccagctccgc cgtggaacac agctcttccc gcagccagcg cagggtcagc cgtgttttct
     8641 ccgcgttccg cgcctgccgc ttcgcgtggc tgacgttgta ctgctccagt gtcatcagta
     8701 aagccccgca aaatcatcat ccgcttttgg tccgccgccg tcggccggag gcaggtactc
     8761 cgccagcggg tccgttttcg ccagcaccgg gaaggttttc agcgaaattt cccggcgctc
     8821 aacccgcagc gggctgctga acgccagccg cgccacgccc agcccggtca tcaccgcgaa
     8881 cccgtcaggc aggtgttgca gggtgttcac gtcaaacagc ggccgcgcct ccttctggat
     8941 gaagctgtcg ccgctgatgt gctccacgtt ccccgcctcc cggctggccc ggcggcgctc
     9001 cacgtccacc cggatctccc cggtctggcc cgccgcccac tgcgcggact cctggctggc
     9061 cgcgcgatag aaccagcgga tcggcgtgtt atccagtacc gtggttttca caaaatcagc
     9121 gggcagatcc tgcccgcact gaccgaagtc gcccagcgac tggtgcgcga gcagcaggtt
     9181 gcagtcccgg ctgcgcagcg tccccagcgc gttcagcacg tacttcgaca gcagatattt
     9241 aatctcatcg agcatgatgc tcgcgtgcgg ccacctccgg aactcatccc gcgcaatgat
     9301 gatttgcgcg cagcgggcaa acagcatctt ctgcacccgg atcaccgcct catcgtccat
     9361 cgagccgatg acgtacaggc agccgccgcc gttgattatc cccgccacat cgataccgct
     9421 gtccgtctgc aggacgctca ggtcggccac gttctccagc ttggtgatca gccccttcac
     9481 gtcctttttc agggcctccg gcaacgcgta cgccgccgcc agcacctgct gaatgtttgc
     9541 cccctgcggg aactgctccg caatcagctt cgccgctttc tgctcgctga tgcggtagtg
     9601 gtccgccacg tcgccggtct cactcaggtt aaacccggca accagcagat tcttcagggc
     9661 gtactggtcg agatcgcgga acagattcag ctgcgccggt ttccccggcc gcagatccag
     9721 cagggtgaag tccgggcagt gcgcccggaa gacgtgcggc gcccacgcgt cccctttggg
     9781 gtcgaagtac accacagcgt ccccgaaatc gcgtacgcag cgaatgagcg cgttggtggc
     9841 catcgcgctc ttgccgctcc cgggcaggcc gaggatctga atattggtct tgcgccaggt
     9901 atcacgaggg accagcaccg gctggccctg ctcatcctta cccagaaaca ccgcctgctg
     9961 tttccgggcc tgctccgtgg aaaaatcggc cagcgcgtta accggtaccg gctccccgaa
    10021 atgcaggtgg cggacgtctg tcatttctcc ggtccgctgg
//
