LOCUS       CP073291                4844 bp    DNA     circular BCT 26-APR-2021
DEFINITION  Klebsiella pneumoniae strain 135077 plasmid pCTXM65_135077, complete
            sequence.
ACCESSION   CP073291
VERSION     CP073291.1
DBLINK      BioProject: PRJNA353728
            BioSample: SAMN18793521
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Zhao,Q., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain 135077
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Zhao,Q., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2021) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/20/2021 11:29:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,740
            CDSs (total)                      :: 5,619
            Genes (coding)                    :: 5,479
            CDSs (with protein)               :: 5,479
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 51 of 140
            Pseudo Genes (incomplete)         :: 87 of 140
            Pseudo Genes (internal stop)      :: 20 of 140
            Pseudo Genes (multiple problems)  :: 18 of 140
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="135077"
                     /isolation_source="urine"
                     /db_xref="taxon:573"
                     /plasmid="pCTXM65_135077"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2020-06"
     gene            join(4829..4844,1..1082)
                     /locus_tag="KC089_26770"
                     /pseudo=""
     CDS             join(4829..4844,1..1082)
                     /locus_tag="KC089_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965681.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /name="TonB-dependent receptor"
     gene            complement(1150..1599)
                     /locus_tag="KC089_26775"
                     /pseudo=""
     CDS             complement(1150..1599)
                     /locus_tag="KC089_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /name="PAS domain-containing protein"
     gene            complement(1679..2383)
                     /locus_tag="KC089_26780"
     CDS             complement(1679..2383)
                     /locus_tag="KC089_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="QUF98179.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            2453..2644
                     /locus_tag="KC089_26785"
                     /pseudo=""
     CDS             2453..2644
                     /locus_tag="KC089_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            2894..3769
                     /locus_tag="KC089_26790"
     CDS             2894..3769
                     /locus_tag="KC089_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-65"
                     /protein_id="QUF98177.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSSG
                     GRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAVAAVLKQSETQKQLLNQPVEIKPAD
                     LVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGDETF
                     RLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNTTGAA
                     SIRAGLPTSWTVGDKTGSGDYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAERRRDVLA
                     SAARIIAEGL"
                     /name="class A extended-spectrum beta-lactamase CTX-M-65"
     gene            3804..4772
                     /locus_tag="KC089_26795"
     CDS             3804..4772
                     /locus_tag="KC089_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="QUF98178.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5-like element IS903B family transposase"
     ARG             2895..3766
                     /name="blaCTX-M-65"
                     /gene="blaCTX-M-65"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-65"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 acgatgacag cggttttatc ccttacgcca tcaccaccgg ccttgattac gaggcgatga
       61 ccgaacagcg caaaggctat gagaactttg tttatcagaa cggcgtttac gatctcggca
      121 ccaaaggtga tatgcgacgt aacgaacgta acctgatgtg gaacctcgat ccctacattc
      181 aaacgcactg gcagctcacc tcagccctgg ggctggacgc cggggcgcgc tatagctcgg
      241 tatggtttga ttccaacgat cgttatgttg taggtaaaaa tggtgatgat agcggcgaag
      301 cgagctatca caagtggctt ccggcggcag cgctgaagta tgccatgacg ccgcagtgga
      361 acgtttacct ctctgccggg aggggatttg aaacgccgac cattaacgag ctgtcctatc
      421 gtcccgatgg tcagtccggg cttaactttg ctctgcaacc ggccactaac accaccgttg
      481 aagcaggcag taaatggcag gtgggcaccg ggatggcgag cctgagcgtc ttcaatacgg
      541 acacgaaaaa tgaaattgtc gttgccgcca gcgacaatgg ccgtacgtcc tatcagaacg
      601 cgggcaagac gcgccgccgc ggggtcgaac tgggatggga tcagcaattt gcgcccgcct
      661 ggcgcgccaa actggcgtgg acgctgctgg atgcgaccta ccgtgaaaac gccggcgatg
      721 ctattcagag cggcaatcgg atcccgggga ttgcacgtaa tagcgcctat gcctcctttg
      781 gctgggtgcc ggaagaggga tggtacgctg gcgctgaagt ccgctatatg agcgatattc
      841 aggtcaacga tgccaatagc gagcaggctc cagcctatac ggtgacggca ctgaataccg
      901 gctataaata tgtgctagat aactggaccg tcgacctcta tacgcgcgtt gataacctgt
      961 ttgataagcg ctacgtcggc tctgttatcg tcaacgagag caacggacgc tattatgaac
     1021 cggcgccggg ccgtaactat ggcgttgggc tatcggtcag ctatcagttt gattcgctgt
     1081 aacaatacgg ctgccggaga gtgctattga ggtgaggctc cggcagcggg taaaggcgtt
     1141 gattcctggt aacaataagc tgcttattga tgtatcagta agcatgccgg cagccattgc
     1201 gcttgatata atactacccc atatgagcaa gagtattttc acggaaaagc tagcagccaa
     1261 tttcggcctc gcggccttcc cgcttctcaa ttgagcatat aatttttccg cagccaaaac
     1321 ctgctcaggt tcaggcttgg tccttacaga ctggtattca acaatcgaac cattcttagc
     1381 tattggcgtt acataagcac ttacccaata gtggtcgcca tttttacagc gattttttac
     1441 tagccccatc catgagcggc cagattttaa tgtactccac atatgctcaa atgcagcagg
     1501 cggcatatct gggtgtctta cgatgttgtg aggctggcct aatagttctt cctcagtgaa
     1561 accactgatt ttaatgaagt caggattaac gtacgtgata tggctttgag gggaggtagt
     1621 cgaaagggca ctgttgcaaa gttagcgatg aggcagcctt ttgtcttatt caaaggcctt
     1681 acatttcaaa aactctgctt accaggcgca tttcgcccag gggatcacca taataaaatg
     1741 ctgaggcctg gcctttgcgt agtgcacgca tcacctcaat acctttgatg gtggcgtaag
     1801 ccgtcttcat ggatttaaat cccagcgtgg cgccgattat ccgtttcagt ttgccatgat
     1861 cgcattcaat cacgttgttc cggtacttaa tctgtcggtg ttcaacgtca gacgggcacc
     1921 ggccttcgcg tttgagcaga gcaagcgcgc gaccataggc gggcgcttta tccgtgttga
     1981 tgaatcgcgg gatctgccac ttcttcacgt tgttgaggat tttacccaga aaccggtatg
     2041 cagctttgct gttacgacgg gaggagagat aaaaatcgac agtgcggccc cggctgtcga
     2101 cggcccggta cagatacgcc cagcggccat tgaccttcac gtaggtttca tccatgtgcc
     2161 acgggcaaag atcggaaggg ttacgccagt accagcgcag ccgtttttcc atttcaggcg
     2221 cataacgctg aacccagcgg taaatcgtgg agtgatcgac attcactccg cgttcagcca
     2281 gcatctcctg cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca
     2341 gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca
     2401 aaaggggatg ataagtttat caccaccgac tatttgcaac agtgccattc ggatttcata
     2461 taacctattt ttgttgttca agtttgattc cttggactct tcagaataca gacagcaaat
     2521 aaagaccttt cgtttgaagt atgtatttct tgcagcaaaa ataatcaaaa ccgcaagata
     2581 tgtaatcatg aagttgtcgg aaaactatcc gtacaaggga gtgtatgaaa aatgtctggt
     2641 ataataagaa tatcatcaat aaaattgagt gttgctctgt ggataacttg cagagtttat
     2701 taagtatcat tgcagcaaag atgaaatcaa tgatttatca aaaatgattg aaaggtggtt
     2761 gtaaataatg ttacaatgtg tgagaagcag tctaaattct tcgtgaaata gtgatttttg
     2821 aagctaataa aaaacacacg tggaatttag ggaatactga tgtaacacgg attgaccgta
     2881 ttgggagttt gagatggtga caaagagagt gcaacggatg atgttcgcgg cggcggcgtg
     2941 cattccgctg ctgctgggca gcgcgccgct ttatgcgcag acgagtgcgg tgcagcaaaa
     3001 gctggcggcg ctggagaaaa gcagcggagg gcggctgggc gtcgcgctca tcgataccgc
     3061 agataatacg caggtgcttt atcgcggtga tgaacgcttt ccaatgtgca gtaccagtaa
     3121 agttatggcg gtcgcggcgg tgcttaagca gagtgaaacg caaaagcagc tgcttaatca
     3181 gcctgtcgag atcaagcctg ccgatctggt taactacaat ccgattgccg aaaaacacgt
     3241 caacggcaca atgacgctgg cagaactgag cgcggccgcg ttgcagtaca gcgacaatac
     3301 cgccatgaac aaattgattg cccagctcgg tggcccggga ggcgtgacgg cttttgcccg
     3361 cgcgatcggc gatgagacgt ttcgtctgga tcgcactgaa cctacgctga ataccgccat
     3421 tcccggcgac ccgagagaca ccaccacgcc gcgggcgatg gcgcagacgt tgcgtcagct
     3481 tacgctgggt catgcgctgg gcgaaaccca gcgggcgcag ttggtgacgt ggctcaaagg
     3541 caatacgacc ggcgcagcca gcattcgggc cggcttaccg acgtcgtgga ctgtgggtga
     3601 taagaccggc agcggcgact acggcaccac caatgatatt gcggtgatct ggccgcaggg
     3661 tcgtgcgccg ctggttctgg tgacctattt tacccagccg caacagaacg cagagcgccg
     3721 ccgcgatgtg ctggcttcag cggcgagaat catcgccgaa gggctgtaac tggttttgtt
     3781 gaataaatcg aacttttgct gagttgaagg atcagatcac gtatcttccc gacaacgcag
     3841 accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gcccacctac aataaagccc
     3901 tcatcaaccg tggctccata actttctggc tggatgatga agctattcag gcctggtatg
     3961 agtcagcaac accttcttca cgaggcagac ctcagcgcta ttctgacctt gccatcacga
     4021 ctgtgctggt cattaaacgc gtattcaggc tgaccctgcg cgctgcgcag ggctttattg
     4081 attccatttt ttctctgatg aacgttccgc tacgctgccc ggattacagc tgtgtcagca
     4141 ggcgggcaaa gtcggttaat atcagtttca aaacgcccac ccggggtgaa atcgcacacc
     4201 tggtaattga ttccaccggg ctgaaggtct tcggtgaagg cgagtggaaa gtcaaaaagc
     4261 atggccagga acgccgtcgt atatggcgta agctgcatct ggcagttgac agtaaaacac
     4321 atgaaatcat ctgcgctgac ctgtcgctga acaacgttac ggactcagag gccttccccg
     4381 ggttaatccg gcaaacccac cggaaaatca ggtcagccgc cgccgatgga gcttacgata
     4441 cccggctctg tcacgatgaa ctgcggcgta agaaaatcag cgcgcttatc ccgccccgaa
     4501 aaggtgcggg ttactggccc ggtgaatatg cagaccgtaa ccgtgcagtg gctaatcagc
     4561 gaatgaccgg gagtaatgcg cggtggaaat ggacaacaga ttacaaccgt cgctcgatag
     4621 cggaaacggc gatgtaccgg gtaaaacagc tgttcggggg ttcactgacg ctgcgtgact
     4681 acgatggtca ggttgcggag gctatggccc tggtacgagc gctgaacaaa atgacgaaag
     4741 caggtatgcc tgaaagcgtg cgtattgcct gaaaacacaa cccgctacgg gggagactta
     4801 cccgaaatct gatttattca acaaagccac gcgctggaca cacc
//
