LOCUS       CP070397                8910 bp    DNA     circular BCT 24-FEB-2021
DEFINITION  Escherichia coli strain CFSAN083829 plasmid pCFSAN083829_4, complete
            sequence.
ACCESSION   CP070397
VERSION     CP070397.1
DBLINK      BioProject: PRJNA230969
            BioSample: SAMN17587898
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Hoffmann,M., Balkey,M., Ronco,T. and Hendriksen,R.S.
  TITLE     Co-localization of colistin and carbapenem -resistance genes on a
            novel transferable IncHI2 plasmid in Escherichia coli from
            chicken-origin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Hoffmann,M., Balkey,M., Ronco,T. and Hendriksen,R.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2021) CFSAN, US FDA, 5001 Campus Drive, College
            Park, MD 20740, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 924x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/17/2021 12:58:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 6,169
            CDSs (total)                      :: 6,045
            Genes (coding)                    :: 5,684
            CDSs (with protein)               :: 5,684
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 149 of 361
            Pseudo Genes (incomplete)         :: 209 of 361
            Pseudo Genes (internal stop)      :: 75 of 361
            Pseudo Genes (multiple problems)  :: 63 of 361
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CFSAN083829"
                     /isolation_source="chicken meat inner strip"
                     /db_xref="taxon:562"
                     /plasmid="pCFSAN083829_4"
                     /geo_loc_name="Romania"
                     /collection_date="2016"
                     /collected_by="DTU, Institute for Hygiene and Veterinary
                     Public Health"
     gene            complement(371..604)
                     /locus_tag="JNP96_30790"
     CDS             complement(371..604)
                     /locus_tag="JNP96_30790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QSA00744.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(611..1129)
                     /locus_tag="JNP96_30795"
     CDS             complement(611..1129)
                     /locus_tag="JNP96_30795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QSA00745.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(1107..1865)
                     /locus_tag="JNP96_30800"
                     /pseudo=""
     CDS             complement(1107..1865)
                     /locus_tag="JNP96_30800"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1807..2130)
                     /locus_tag="JNP96_30805"
     CDS             complement(1807..2130)
                     /locus_tag="JNP96_30805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QSA00751.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2195..2386
                     /locus_tag="JNP96_30810"
     CDS             2195..2386
                     /locus_tag="JNP96_30810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="QSA00746.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3056..3214
                     /locus_tag="JNP96_30815"
     CDS             3056..3214
                     /locus_tag="JNP96_30815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QSA00747.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="replication initiation protein"
     gene            complement(3411..3548)
                     /locus_tag="JNP96_30820"
     CDS             complement(3411..3548)
                     /locus_tag="JNP96_30820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="QSA00748.1"
                     /translation="MRKRFFVGILAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            3596..3937
                     /locus_tag="JNP96_30825"
     CDS             3596..3937
                     /locus_tag="JNP96_30825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="QSA00749.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLP
                     GLFISIKNN"
                     /name="colicin transporter"
     gene            complement(3934..5499)
                     /locus_tag="JNP96_30830"
     CDS             complement(3934..5499)
                     /locus_tag="JNP96_30830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-10"
                     /protein_id="QSA00750.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-10"
     gene            5777..6196
                     /locus_tag="JNP96_30835"
     CDS             5777..6196
                     /locus_tag="JNP96_30835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="QSA00742.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="entry exclusion protein 1"
     gene            6280..6699
                     /locus_tag="JNP96_30840"
     CDS             6280..6699
                     /locus_tag="JNP96_30840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="QSA00743.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSNVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7225..7401
                     /locus_tag="JNP96_30845"
                     /pseudo=""
     CDS             7225..7401
                     /locus_tag="JNP96_30845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          complement(2472..2559)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.753"
                     /coverage="100.0"
                     /evalue="1.48e-35"
     Typing          2582..3128
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(268..1817)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.656"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctgatacca tcagaagaag cattaaacgc attctggcgt cgattacaga agaagcggaa
       61 cgcgttccag gctcctgttg ctatacacac tacccacact aaccccatgg tttttgagcg
      121 ccagaaacgc gtccgtatgc gttttaacgc gttcctgtgt tttcagcaag gcctgactga
      181 agctagttcc tagtgccctg aaccaaccat tatccaaaaa aatgcaccgg aaacgcgctg
      241 acaacacttt agcctgtctt tggttaccat cccagcgacg ggccatccag tgtccgttcc
      301 tgtatttcca gatggcgctc acgctctagg aatagctcat gttcacgttc ctgtatcagt
      361 agttcgtccc ttatctcctg ctctcgctgt ataactggct caagtgttct gtctgctcgc
      421 tcaagtgctg cacctgctga ctcaactgca tgacccgctc gttcagcatc gcgttgtccc
      481 gttgcgtaag cgaaaacatc ttctgcaatt ccacgaaggc gctctcccat tcgttcagcc
      541 gctgcatata gtcctgttgc agctgctcta aggcgttcag caaatgtgtt tccagctctg
      601 tcactctgtg tcactccttc agatgtaccc actctttccc ctgaaaggga atcacctctg
      661 ctgatttccc gtacggaagg accagaaatt tcctgttccc gtcctgcacg aactccacgc
      721 cccatgtctt cgcgttcagt ttctgcaatg tctcttcctg cttcctgata tcttccaggt
      781 tcgcctgtat cctcccgcca agataccaga gcgtcccgcc actcgcggta aacaggagaa
      841 agactatccc cagtaacatc atgcccgtat tccctgccag ctttaacacg tccttcctgt
      901 gctgcctcat cgcctctttc accccttccc ggtgtttctc cagcgattcc tctgtcgagg
      961 ctgtgaacag ggctatagcg tctctgattt tcgtctcgtt tgatgtcaca gcctcgctta
     1021 cagattcgcc gagcctcctg aactcgttgt tcagcacttt ctctgtagat tcggctctct
     1081 ctttcagctt tttctcgaac tccgcgcccg tctgtaaaag attgctcata aaacgctcct
     1141 ttcagcctga tattcttccc gccgttcggg tctgcaatgc tgatgctgct tcgcgtcacc
     1201 ctgaccactt caagccccgc ctctgtgagc gcctgaatca catcctgacg gccttttatc
     1261 tcccctacat gataaagagc gtctatcccg cgtgtgacgc tctcagcaag cgcctgtttc
     1321 gtttccggca ggttatcaga gagagtcagt atccggcggt tctccggggc gttggggtca
     1381 tgcagcccgt aatggtgatt caccagcgtc tgccaggcat caattctcgg cctgtctgcc
     1441 cggtcgtagt acggctggag acgttttccg gtctgtagct ccatgttcgg aatgacaaaa
     1501 ttcagctcaa gccgtccctt gtcctggtgc tccacccaca ggatgctgta ctgattcttt
     1561 tcgagacctg gcatcagtac gcgctcaaag ctcgccatca ctttttcacg tcctcccggc
     1621 ggcagctcct tctccgcgaa cgacagaacg cctgacgtgt atttcttcgc aaatggcgtg
     1681 gcatcgatga gttcccggac ttcttccgga ttaccctgaa gcaccgttgc gccttcgcgg
     1741 ttacgctccc tccccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca
     1801 tgaaatttaa ctatcatccc gcgccccctg ttccctgacg gccagacgca gccggcgcag
     1861 ctcatccccg atggccatca gtgcggccac cacctgaacc cgatcaccgg aagaccactg
     1921 cccgctgttc accttcctcg cggtctgatt caggttattc ccgatggcgg ccagctgacg
     1981 cagtaacggc ggtgccagtg tcggcagctt cccggaacgg gcaaccggct cccccaggca
     2041 gacccgccgc atccataccg ccagttgttt accctcacag cgttccagta accgggcatg
     2101 ttcatcatca gtaacccgta tcgtgagcat cctctcgcgt ttcatcggta tcattacccc
     2161 catgaacaga aatccccctt acacggaggc atcagtgact aaacaggaaa aaaccgccct
     2221 taacatggcc cgcttcatca gaggccagac attaacgctg ctggagaaac tcaacgaact
     2281 ggacgcggat gaacaggcag atatctgtga atcgcttcac gaccacgccg atgagcttta
     2341 ccgcagctgc ctcgcacgtt tcggggatga cggtgaaaac ctctgacaca tgcagctccc
     2401 ggagacggtc acagcttgtc tgtgagcgga tgccgggagc cgacaagccc gtcagggcgc
     2461 gtcagcaggt tttagcgggt gtcggggcgc agccctgacc cagtcacgta gcgatagcgg
     2521 agtgtatact ggcttagtca tgcggcatcg gtgcggattg tatgaaaagt gcaccatgta
     2581 cggtgtgaaa tgccgcacag atgcgtaagg agaacatgca gatgccgatg ctcttccgct
     2641 tcctcgctca ctgactcgct gcgctcggtc gttcggctgt ggcgagcggt gtctgctcac
     2701 tcaaaagcgg tgatactgtt atccacacaa tcaggggata acgccggaaa gaacatgtga
     2761 gcaaaaaacg aagaccccag aaaaggccgc gccggaggcg ctttttccat aggctccgcc
     2821 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2881 ttaaagatac caggcgtttc cccccggaag ctccctcgtg cgctctcctg ttccgaccct
     2941 gccgcttacc ggatacctct ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3001 ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3121 cccggtaaga cacgacaaag cgccactggc agcagccgtt ggtaacttgg ttagcagagc
     3181 gatgatggcg caaatggtgc aacagagttc ttgaagtagt ggcccgacta cggctacact
     3241 ggaaggacag tatttggtat ctgcgctctg ctgaagccag ttacctcggt taagcagttc
     3301 cccaactgac ttaaccttcg atcaaaccgc ctccccaggc ggttttttcg tttacaggca
     3361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctactgaaca
     3421 gagatccccg tcagtttaga agaagatgat ggagccacag tccctccctg aacatcacgt
     3481 atatagttag cctgacatcc aacaaggagg tttatagcta atattcccac aaaaaatctt
     3541 ttcctcataa ctcgatcctt ataaaatgaa aagaatatat aacgaggttt aatttatgag
     3601 tttaagatac tacataaaaa acattttgtt tggcctatat tgcacactta tatatatata
     3661 ccttataaca aaaaacaacg aagggtatta tttcctagcg tcagataaga tgctatacgc
     3721 aatcgtgata agcactattc tatgcccata ttcaaaatat gctattgaac acatattttt
     3781 taagttcata aagaaagatt ttttcagaaa aagaaaaaat ctaaataatg cccccgtggc
     3841 aaaattaaac ctatttatgc tatacaatct actttgtttg gtcctagcaa tcccatttgg
     3901 attgccagga ctgtttatat caataaagaa taattaaata cccaacacat catttatagt
     3961 attaagttta ttcttgtcaa taaaggcaca tagaatgcct gtaacaatag caatccccca
     4021 gatacctaat gtagttccag caagcacact aaaaagtaaa acaacaatat aactaactcc
     4081 agcatctaca gttttcttct ctaatgtaag aaatagtggc ttccagtcac ctgtatcctt
     4141 aatttttagg atatcagata ccacatcata tccaaaagaa acatgtcccg taatcttcaa
     4201 gtacttagca aactgatcta aatgcttagc ccagtcttca tacttaaccg attccagtgc
     4261 attgaaaatc gcatcacggt ctgctttgct gaatttctta tttaaaacat ccttgtattt
     4321 ttcaaaagca gctagagctt cattcacatt gctgattttc ttacctttag acttatcagc
     4381 aagttcctgt gccatttttg aatatttttc accatatttt tcagatagcg tttgataaaa
     4441 gctaactgtt gcatccacag catccttaat ctgcgagttc aggagattag tctgcgctgt
     4501 tttcaaattt tcttcagatt catgaacacg taaaacagct gtattacgat tattattagc
     4561 ctgagacata gccccctgaa ttccatttat ctcagcatta agttggttaa tacgcgtttc
     4621 tgatgctgtt acctgttttt gtttttcctc cataatctta ccggctcccg tccgtcgact
     4681 ggtggcatag aaaaaaggac gactctgaag cggatcatta gctcttggtg acaatttttt
     4741 gaccagttca tcaagttcct tatatttagc agatgcctga gccagctcat ttcgttttcc
     4801 ggacagcgta ttcatttctg catcacgggc atgaatactg gagcttaaac gggtattgag
     4861 agtcttaatc tcttcatcca ctttcgccag ctcagattgt gcagcagcaa gatttttttg
     4921 tgccacctcc actgcccggg cctcttcatt caatgctgcc agccgtttct cttcagcttc
     4981 agccagtttc aactggcgtt ctgtttcagc ctgctccttc tctatctctt tacgtcgttg
     5041 ttctgcctcc tgaaaagcct tttccgcagc ttccgcttct ttacgggctt tttcttctgc
     5101 ttttgcaagg cgcaaccgct ctgcttctgc ctgcatcgct gcattattgg catgagccag
     5161 gtcaataacc tccggatgag tggaattatg acgaagcgcc tcattcacaa tatccttcag
     5221 atgttgagtc agcgcatccc ggtttgcttt tgctttagcc tgtgcttctg cggcagcttt
     5281 tgcccgggca gcctgttctg cctgcgtttt cttcaattga gcagtagacc atttagctgt
     5341 ggcatgaatg gctgcagaac tttcactttt gctacctcca gttccaccac cgccgccaga
     5401 gccactcccg tctggattac cattcaaaag agtaatgatt acctgcccct tatcatcata
     5461 aggaacacca tctttatagt acgctacagc tgtttccatt ataaaatcct ctttgacatt
     5521 aaaaacaaat agttaaaagc aatatactgt atatacaacc actggtttta tatacagctt
     5581 aaaattacgc cgctgcattt tccctgtcaa ccctgtggat tttcattttt gtgaaaacga
     5641 tcaaaaaaac agccacccac accacgtgct attcccaccc gatgccacaa aaactagcac
     5701 aaacattacc gttctccggc cccagtatgt tttactgaaa ccatgatatg agacattaat
     5761 gggacactgt cacttcatgg catggcacac actccagaac gcactgaaaa tgacaggcag
     5821 atcgcgttca cagttttacc gtgatatgcg cgcaggcctt gtcagttatc gtacaggcca
     5881 ggacggacgg cgggagtttg aaaccagtga actgatccgg gcatacggcg aattaaagca
     5941 ggatgagaca ccagaaaggc acagtgcggg acaggcagaa aatccacatg atcagcagac
     6001 agaacgcatt ctccgggagc tgaatgagct gagacaatgc ctgacactga tgcttgagga
     6061 caaacaggca caggctacgg aacgccggcg ccaggaagcc gaacgggaac agctacataa
     6121 agagatagag cagctcaggc aggcactgga gcaggaaaaa aaacggggat tctgggccag
     6181 actgttcagc cggtgagcac cgactgcaag tgatgataag atagtcagcg gttaaatata
     6241 agtcactgag tacaaagatt taaaggtaag gagacactca tgagacgttc cggcggcaca
     6301 ctgtatctta tcgccctgct gacagcggca acggtcctga cagcctgcac gccaaagggc
     6361 agtgtggaac aacatacccg gcattacgtt tatgcatcag acgatggttt tgatcctaac
     6421 ttttccaccc agaaggccga cacaacacga atgatggtgc cttttttccg gcagttctgg
     6481 gatatgggag caaaggataa agcgacagga aaatcacgga gtaatgtgca gcaacgcatt
     6541 cagcagtttc acagccagga atttttaaac tcactccggg gcacaactca atttgcgggt
     6601 actgattacc gcagcaaaga ccttaccccg aaaaaatcca ggctgctggc tgagacgatt
     6661 tctgcggttt atctcgatgg ctacgaggga agacagtaag tggatttacc ataatccctt
     6721 aattgtacgc accgctgaaa tgcattcagc gcaatcacgg cagcagacag gtaaaaatcg
     6781 caacaacccc cccgaaaaac taccgcgatc gcgcctgata aattttaacc ttatgcatac
     6841 ctatgcatcc aggtgaatca caagcgaatt tccttactga gacgactgaa acggtttttt
     6901 ttcacaattt tcagctgttt tcgattccac aaaatagaag ttacaggata gggattaatc
     6961 cccttcgccg ccaaggggag caggatgagt caggggaatg tctattcacc tgcgctgtac
     7021 aggctgccag gtaaaagtat tcgctgcggc acgatactct tttgccctca tagcctttgc
     7081 gcttcttact tttcgttctt ccagaatcat tcttaagaca ttttcccgca cttttctcta
     7141 acttcccgtg gcaatttatc tctgccggac tgaaccgccc cggaaatcct ggagactaaa
     7201 cttcctgaga aagatgtaaa caggatgact aaaaatactc gtttttcccc cgaagtccgt
     7261 caacgggcag ttcgtatggt tctggaaagc cagggcgaat atgactcaca atgggcggca
     7321 atttgttcca ttgctccaaa gattggctgt acgccagaga ccctgcgtgt ctgggttcgc
     7381 cagcatgagc gggataccgg gtattacctt tcgaagaaat ttttgacatc ctctacgccc
     7441 tgaaggaccg ggttttacgg cgtaccagat aaaaaaaccc ctctactgaa gaggggtagt
     7501 gtaaggaact tgctgatttt cttgatatta ttaaccatca ataactgagc atacaaaaaa
     7561 taacaaaact ctgacacaga tacaaccatc cctctttgta taagaccgat gtgcatctca
     7621 aaaaagaagc gtgtttctgg aacacagaac aacaatgcac attgtagcca acaaattcac
     7681 accagaaaca cgctctgttt tttaatttac acaagaatat atgtctggaa aatcaggaag
     7741 aagaaattaa tgggcattct gtatacgaag ggagagcgcg tttccggtgc cccatattag
     7801 atattatccc tgcaacaaac accagagacg cgctcataat tcttctagcg taaatgatcc
     7861 tttcaggcaa gaaaaaagcc gtgattctgt atcacggcct taattttatc gaaaacacgg
     7921 attttagtat gcacatgact gtactcctgt ggagtacaag gaaatcataa tcagcgacca
     7981 aaccatttat caacagaaaa ccaacatatt gatgttattt ttgtgacaaa tgttacaaat
     8041 tcgaaaccac tgtattttta ctgcaacaaa atctggttga tctacaagtt acactggggt
     8101 aaaaagttgt ccggaatgtt tctgcgtagt aaagcctcta gccggattgc ctttgcaaaa
     8161 acgcaggaac gttcccccct tcatcagtac tcctgacgct gtaaggagct gactgatgca
     8221 gacagggctg atgatatcta ataaaagcta tctcattttt gcgcgtacat aaaataaatc
     8281 aggcacttcc ctacactccc cctccggaat tgagggggtt ttctttacaa tgtaaataaa
     8341 ttgcttgcgt aaagttcaaa aaaactgcaa atttttactg tagtgtcgag cttcatgtag
     8401 ctcaccaggg catggactaa aaagaagtaa acccatcttt taccccccct tatacggggg
     8461 gttctttata gcacaaagaa cacttataat ttatcatctc acagctataa atataaaaga
     8521 ataatattct aaactacagt tatttatgca gaacataaca tcaaactcac aataaatata
     8581 aaacaattat tttataactg ttcacttatt atattcagac atatcagaaa cacgactcaa
     8641 ttctttttta tttaacaaaa tccataaaaa acttatggtt gaaatcacat ttaaactgtg
     8701 aaggatatca tccggagaaa tttttcaatc cgacactcca tagaatggac ctttaagact
     8761 ttatcctcat catgtatgag gatttttttt ctggcaaaca gtatctttca gtaatccttg
     8821 ctcagttccg attctgacgt atccccgtgt ggatctgaaa aggagataat tacgctctgt
     8881 ggccaacagg aataactcaa gcagctcctc
//
