LOCUS       CP068406                3223 bp    DNA     circular BCT 25-FEB-2021
DEFINITION  Serratia marcescens strain MRSN696556 plasmid unnamed7, complete
            sequence.
ACCESSION   CP068406
VERSION     CP068406.1
DBLINK      BioProject: PRJNA692233
            BioSample: SAMN17317342
KEYWORDS    .
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Corey,B.W., Lebretron,F., McElheny,C.L., Lovleva,A., Ong,A.C.,
            Maybank,R., Margulieux,K., Cybulski,R.J., Kwak,Y.I., Mc Gann,P.,
            Doi,Y. and Bennett,J.W.
  TITLE     KPC-76 is a KPC-2 variant carried by a Tn4401 transposon and
            conferring resistance to ceftazidime/avibactam in Citrobacter koseri
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Corey,B.W., Lebretron,F., McElheny,C.L., Lovleva,A., Ong,A.C.,
            Maybank,R., Margulieux,K., Cybulski,R.J., Kwak,Y.I., Mc Gann,P.,
            Doi,Y. and Bennett,J.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2021) Multidrug-Resistant Organism Repository and
            Surveillance Network (MRSN), Walter Reed Army Institute of Research,
            503 Robert Grant Avenue, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-AUG-2020
            Assembly Method                   :: Unicycler v. V0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Oxford Nanopore Minion/Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/21/2021 13:26:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,633
            CDSs (total)                      :: 5,506
            Genes (coding)                    :: 5,342
            CDSs (with protein)               :: 5,342
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 47 of 164
            Pseudo Genes (incomplete)         :: 116 of 164
            Pseudo Genes (internal stop)      :: 12 of 164
            Pseudo Genes (multiple problems)  :: 10 of 164
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="MRSN696556"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: Texas"
                     /lat_lon="29.46 N 98.41 W"
                     /collection_date="17-Oct-2019"
                     /collected_by="Brooke Army Medical Center"
     gene            join(3197..3223,1..459)
                     /locus_tag="JMM80_28140"
     CDS             join(3197..3223,1..459)
                     /locus_tag="JMM80_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QSD90176.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSTMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            463..678
                     /locus_tag="JMM80_28145"
     CDS             463..678
                     /locus_tag="JMM80_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QSD90177.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            846..1178
                     /locus_tag="JMM80_28150"
     CDS             846..1178
                     /locus_tag="JMM80_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="QSD90178.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            2196..2519
                     /locus_tag="JMM80_28155"
     CDS             2196..2519
                     /locus_tag="JMM80_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QSD90179.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2458..3219
                     /locus_tag="JMM80_28160"
                     /pseudo=""
     CDS             2458..3219
                     /locus_tag="JMM80_28160"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     ARG             847..1178
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          1909..2100
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          complement(1446..1926)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.867"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          4..776
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.077"
                     /coverage="99.0"
                     /evalue="3.74e-142"
     Typing          2510..3222
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.798"
                     /coverage="99.0"
                     /evalue="2.98e-154"
ORIGIN
        1 gacttagagc agaaatcgaa agcgcagcag cagactaccg gcgagatgct gaaagccgca
       61 ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgagaa gagaatcagc
      121 gccgccatcc tcgaccacga ccggaagctg tcctcaacca tgagccagcg cacgaaaggg
      181 atgctgcgca tggtcagcca gacgtggctg accatcgttc tggtctccgc gctgctgata
      241 gcgtcgagcg cgggcattct gtggtggcag gggcagcaga tactcgagaa ctatacgacc
      301 atccgggagc agaagagcac gcaggccatg ctgtcagaga ggaacagcgg cgtacagctc
      361 tcgacctgcg gcgagcagag acgccgctgc gtgagggtga acccggaagc gggacagttc
      421 ggagaggact cgagctggat gatactggcg gggaaatagc acatgacgga gctggaaaaa
      481 cagttgctga gcgcattaga gcagctacag caggactact cgaaaaggct ggacgagtgg
      541 gagaacgcct tcgcggaatg gcggacgatg tctggtctta ttcaacggga gaacgcggcg
      601 ctgaacgagc gcgtcacggt cttgagcagg caggtgcaga gtttgagcga gcagctgcgc
      661 cggttgtcga aaggctgaac gcgatagaaa ctcaccggca gcaggagcgg gcggtgcagc
      721 accagaaagc gctggagctg gaacgatcgc agtggcagca tcatggtcct tcgctatgat
      781 aagccgggtt ctattaatta gttgttatgt aaagatattt aaatttttca acatgaggta
      841 tttttatgaa tacatatatt ttcctgggag gggctattgt ggctgaagtc ataggcacca
      901 cctttatgaa atattcagaa ggatttacac gcctgtggcc gtctgtagga actgcaataa
      961 gttattgcgc tgccttttat ctgctttccc aaacattgga aaatatacct accggtattg
     1021 cttacgcaat ttggtctggt gcgggaattg tactaataag cctagctggt tggttggtta
     1081 gcggtcaaaa acttgattgg cccgcaattg caggaatgtt ccttatttgc gttggagttt
     1141 tggttattaa tattttttca aaatcttctg cgcattaact ttttaccgaa gcatattggc
     1201 caaagcaaaa ggatttcctt gagatccttt ctttctgcgc gtaatctttt tccctgtaaa
     1261 caaaaaaacc accgctacca acggtggttc gtttgccgga attcagagct accaactctt
     1321 tgttccgatg gtaactggct ttcacggagc gcagatacct aaactgtcct ttcagtgtag
     1381 ccgcactcag gctttcactt caagaactct gtaggcattc tctacaatct ctgctccgtg
     1441 aacccagtta ccaatggcta ccgccactgg cgatttgtcg tgtctttccg ggttggactc
     1501 aagttgatag ttaccggaac gggcgtggta gtcggactga acggggggtt cgtgcataca
     1561 gtccatcctg gagcgaactg cctacccgga accgagtttc aggcgtggaa tgagaaaccg
     1621 cggccataac agcggaatga caccggtaaa ccgaaaggca ggaatgcggg ggagcacgag
     1681 ggagccacca gggggaaacg cctggtatct ttaagccgca tcgggtttcg ccaccgctga
     1741 tttgagcgtc attttcgtga tactcgtcag gggggcggag cctatggaaa aacggcaact
     1801 ccgcatcctt ttcttggcct ttcctgcttc gagcggcatt tgcttatccc gtaatctgtg
     1861 agctggtaac gcccgccgca gtctcacggc aggagcgtag cgaccgagtg agcgaggagg
     1921 cgtcatttca tcaccacctg cattttgcac ttacgcccgg cagccgcatt tttcccctgt
     1981 cacatgaagc actttcataa attgccactc cgtcataaca tgtttagcca gtatacactc
     2041 cgctagcgct acgtgactgg ttcagggctg cgccccgaaa cccgctaaaa ccactgccgc
     2101 gcctgcggct tgtccaacac cgtgccgacc gggaaaagat ttcccgtccg tcccggcgtt
     2161 atcaggaggc cggattggca gacaaacgca gcaaaatgct caccatgtgg gtgactgagg
     2221 acgagcaccg gcgtctgctg gagcgctgcg agggtaagca actcgcggcc tggatgcggc
     2281 agacgtgcct ggacgagaag cccgcacgcg ccggcaaact tccgtcgatc tcgccggcgc
     2341 tgcttcgtca gcttgccggc atggggaaca accttaacca gattgcccgc caggttaacg
     2401 ccggtggtgg aagcggacac gaccgcgtgc agattgtcgc cgcgctgatg gccatcgatg
     2461 ccggactcga gcggttgcgg catgccgtac tggaaaaggg tgctgatgat gatcgttaag
     2521 tttcatcctc gggggcgcgg cggcggcggc gggccggtcg attatctgct gggtaaagat
     2581 cgccagcgcg acggcgccag cgttctgcag gggaagccgg atgaagtccg ggagcttatc
     2641 gacgcctcgc cctacgccaa aaagtacact tccggcgttc tgtcttttgc cgaacaggat
     2701 ttaccgcccg gccagcgcga aaagctgatg gcgagtttcg agcgggttct gatgcccgga
     2761 ctcgataaag accagtacag cgtgctgtgg gttgagcacc gggacaaggg gcggctggag
     2821 ctgaacttcc tgatcccgaa cacggagctt ctgaccggca agcggctaca gccgtattac
     2881 gaccgcgccg accgtccgcg catcgatgcc tggcagacca tagtgaacgg caggctgggg
     2941 ctgcatgacc cgaacgcgcc ggagaaccgg cgggtgctgg tctcgccttc cgcgctgccg
     3001 gaagcgaagc aggaagccgc ccaggcgatt acgagcggcc tgcttgccct tgcctcatcc
     3061 ggggagctga aaacgcgtca ggacgtcact gaggcgctgg aaagcgcagg ttttgaggtg
     3121 gtgcgcacca caaaaagcag catcagcatt gccgacccgg acggggggcg aaacatccga
     3181 ctgaagggag ccatctatga acagtctttt aacgctggcg aag
//
