LOCUS       CP068405                5089 bp    DNA     circular BCT 25-FEB-2021
DEFINITION  Serratia marcescens strain MRSN696556 plasmid unnamed6, complete
            sequence.
ACCESSION   CP068405
VERSION     CP068405.1
DBLINK      BioProject: PRJNA692233
            BioSample: SAMN17317342
KEYWORDS    .
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Corey,B.W., Lebretron,F., McElheny,C.L., Lovleva,A., Ong,A.C.,
            Maybank,R., Margulieux,K., Cybulski,R.J., Kwak,Y.I., Mc Gann,P.,
            Doi,Y. and Bennett,J.W.
  TITLE     KPC-76 is a KPC-2 variant carried by a Tn4401 transposon and
            conferring resistance to ceftazidime/avibactam in Citrobacter koseri
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Corey,B.W., Lebretron,F., McElheny,C.L., Lovleva,A., Ong,A.C.,
            Maybank,R., Margulieux,K., Cybulski,R.J., Kwak,Y.I., Mc Gann,P.,
            Doi,Y. and Bennett,J.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2021) Multidrug-Resistant Organism Repository and
            Surveillance Network (MRSN), Walter Reed Army Institute of Research,
            503 Robert Grant Avenue, Silver Spring, MD 20910, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 19-AUG-2020
            Assembly Method                   :: Unicycler v. V0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100X
            Sequencing Technology :: Oxford Nanopore Minion/Illumina Miseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/21/2021 13:26:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,633
            CDSs (total)                      :: 5,506
            Genes (coding)                    :: 5,342
            CDSs (with protein)               :: 5,342
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 47 of 164
            Pseudo Genes (incomplete)         :: 116 of 164
            Pseudo Genes (internal stop)      :: 12 of 164
            Pseudo Genes (multiple problems)  :: 10 of 164
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="MRSN696556"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: Texas"
                     /lat_lon="29.46 N 98.41 W"
                     /collection_date="17-Oct-2019"
                     /collected_by="Brooke Army Medical Center"
     gene            complement(join(4083..5089,1..64))
                     /locus_tag="JMM80_28110"
     CDS             complement(join(4083..5089,1..64))
                     /locus_tag="JMM80_28110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="QSD90174.1"
                     /translation="MKAMVFYGENDVRFEERKKPEIINPGDAIIRMIKTTICGTDLGIL
                     KGKNPEIEKNAKEKTGSFNGRILGHEGVGIIEEIGSAVKNFKIGDKVIISCVSRCGTCD
                     NCQKQLYAHCRNDGGWIMGYMIDGTQAEFVRTPYADNSLYLFPDELNDDAGVLLSDALP
                     TAHEIGIQNGMVKPGDTVAIVGAGPVGMSCLLTGMLYSPSQLIMIDMDENRLAMAKDMG
                     ATHTINSAKENAINRVMELTNQRGVDCAIEAVGIEPTWNICQNIIKEGGHLANVGVHGN
                     PVSFAIEKLWIKNLTITTGLVNANTTGMLMKTCCSGKLATEKLATHHFNFNDIEKAYDV
                     FKHAADEKAMKVMINF"
                     /name="zinc-dependent alcohol dehydrogenase family protein"
     gene            163..860
                     /locus_tag="JMM80_28115"
                     /pseudo=""
     CDS             163..860
                     /locus_tag="JMM80_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            2034..2357
                     /locus_tag="JMM80_28120"
     CDS             2034..2357
                     /locus_tag="JMM80_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="QSD90175.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARACKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSAHDRVQIVSALMAINAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2296..3060
                     /locus_tag="JMM80_28125"
                     /pseudo=""
     CDS             2296..3060
                     /locus_tag="JMM80_28125"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3035..3520
                     /locus_tag="JMM80_28130"
     CDS             3035..3520
                     /locus_tag="JMM80_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="QSD90172.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3524..3733
                     /locus_tag="JMM80_28135"
     CDS             3524..3733
                     /locus_tag="JMM80_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="QSD90173.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1844..1926
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          complement(1282..1759)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.683"
                     /coverage="92.0"
                     /evalue="4.61e-163"
     Typing          2348..3843
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.387"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctctggttt ttttcgttcc tcaaaacgaa catcattttc accataaaaa accatagctt
       61 tcatttgtat atcaccttat gcatccgttt actttgtttg tatattgggt gatgctgcca
      121 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
      181 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
      241 catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc
      301 gcttctcaac ccggtacaca ccagaaaatc attgatatgg ccatgaatgg tgttggatgc
      361 cgggcaaccg cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac
      421 tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgatgtgatt gtctgcgctg
      481 aaatggacga acagtggggc tacgtcggcg ctaaatcacg ccagcgctgg ctgttttacg
      541 cgtatgacag gatacggagg acggttgttg cgcacgtatt cggtgaacgc actatggcga
      601 cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg atgacggatg
      661 gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc aagcgatata
      721 cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg ctgggacgga
      781 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
      841 acataaaaca ctatcaataa gttggagtca ttacctgtat attgtatcat ttgtttgttt
      901 tttgttgttt tttttgtgct ttttgtgctt tttgtgcttt ttgtgctttt tgtgcttttt
      961 gtgctttttg tgctttttgt gctttttgtg ctttttgtgc tttttgtgct ttttgtgctt
     1021 atgtcgttgg ggctgggcgg gggcgaaaat ctggaaagcg aatcagatct ttgtgagaac
     1081 cttcttgaga tccttttttt ctcgtcgtaa tcttatggtc agtaaacgaa aaaaccgccc
     1141 agcaaggcgg ttttttcgaa ggttcttaga gcagcaactc tttgaaccgc ggtaactggc
     1201 ttggaggagc gcagtcacca aaacatgtcc tttcagttta gccttaactg gctcataact
     1261 tcaagactaa ctcctctaaa tcagttacca gtggctgctg ccagtggtgc ttttgcatgt
     1321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcagtc ggactgaacg
     1381 gggggttcgt gcatacagtc cagcttggag cgaactgcct tcccggaacc gagtgtcagg
     1441 cgtggaatga gaaaccgcgg ccacaacagc ggaatgacac cggcaaaccg gatggcagga
     1501 acaggagagc gcacgaggga gccaccaggg ggaaacgcct ggtatcttta agtcctgtcg
     1561 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     1621 tatggaaaaa cggcaacgcc gcatcctttt tccggccctt actgatccct cgctattccg
     1681 tcatcctgtt ctccgtgagt cggtaacgcc cgccgcagcc tcatgaccga acgtagtgag
     1741 cgaatgagcg aggaggcgta atttcatatg tccgtgcatg ctattgcgga cgctcctgaa
     1801 ctacatatca cacctgtcac atgaagcact ttcctgatta cagcactcat gccaacatat
     1861 taagccagta tacactccgc tagcgctacg tgactggttc agggctgcgc cccgaaaccc
     1921 gccaaaagcc actgacgcgc ctgcggcttg tccaacaccg cgccgatcgg gaaagtattt
     1981 cccgcccgtc ccggcgttat caggaggcct gattggcaga taaacgcagc aaaatgctta
     2041 cgatgtgggt gactgaggat gagcaccggc gtctgctgga gcgctgcgac ggtaagcagc
     2101 tcgcagcctg gatgcggcag acgtgcctgg acgagaagcc ggcacgcgcc tgcaaacttc
     2161 cgtcgatctc gccggcgctg cttcggcagc tcgccggtat gggtaacaac ctcaaccaga
     2221 ttgcccgcca agttaacgcc ggtggcggca gcgcacacga ccgcgtgcag atagtctccg
     2281 cgctgatggc cattaatgcc ggactcgagc ggctgcggca tgccgtactg gaaaagggag
     2341 ctgacgatga tcgttaagtt tcatccccgg gggcgcggcg gcggggccgg gccggtggat
     2401 tatctgctgg gaaaagaccg ccagcgcgaa ggtgccagcg ttctgcaggg taaaccggag
     2461 gaagtccggg agcttatcga cgcctcgccc tacgccaaaa agtacacctc cggcgtcctg
     2521 tcgtttgccg aacaggactt actgcccggc cagcgcgaaa agctgatggc gagcttcgag
     2581 cgggttctga tgcccggact cgataaagac cagtacagcg tgctgtgggt tgagcaccgg
     2641 gacaaggggc ggctggagct gaacttcctg atcccgaaca cggagctgct gaccggcagg
     2701 cggctccagc cgtattacga ccgcgccgac cgtccgcgca ttgacgcctg gcagaccata
     2761 gtgaacggca ggctggggct gcacgacccg aacgcgccgg aaaaccggcg ggcgctggtc
     2821 acgccttccg cgctgccgga agcgaagcag gaagccgctc aggcgattac gcggggctta
     2881 ctggccttag cctcgtccgg ggagctgaaa acccgtcagg acgtcactga ggcgctggaa
     2941 agcgcaggtt ttgaggtggt gcgtaccacg aaaagcagca tcagtattgc cgacccggag
     3001 ggggggcgaa acatccgact gaagggagcc atctatgaac agtcttttaa cgctggcgaa
     3061 ggacttagag cagaaatcga aagcgcagca gcagagtacc ggcgagatgc tgaaagccgc
     3121 attcagcgag cacgagaagt ctgtcagagc ggaactgagc gaaagcgaga agagaatcag
     3181 cgccgccatc ctcgaccacg accggaagct gtcctcagcc atgagccagc gtacgaaagg
     3241 gatgctgcgc atggtcagcc agacgtggct gactatcgtc ctggtgtccg cgctgctgat
     3301 agcctcgagc gcgggcattc tgtggtggca gagtcagcag atactcgaca attacacgac
     3361 catccgggag cagaagagca cgcaggcaat gctgtcagag aggaacagcg gcgtgcagct
     3421 ctcgacctgc ggcgagcaga gacgccgctg cgtgaaggtg aacccggaag cgggacggtt
     3481 cggagaggat tcgagctgga tgatactggc ggggaaatag cacatgacgg agctggaaaa
     3541 acagttgctg agcgcattag agcagctaca gcaggactac tcgaaaaggc tggacgagtg
     3601 ggagagcgcc ttcgcggaat ggcggacgat gtctggtctt atgcaacggg agaacgcggc
     3661 gctgagcgag cgcgtcacgg gcttgagcag gcaggtgcag agtttgagcg agcagctgcg
     3721 ccggttgtcg taatgctgaa cgggatagag gcacaccggg agcaggagcg ggcggcgcag
     3781 catcagaaag cgctggagct ggagcgatct cagcgacagc ggacgtataa cggcccttca
     3841 ctgtgataac cggataaaat ggggctttcg gcggacatct gcgctgaaag ccggagccat
     3901 tcacccggct gcatggctat gcagtccggg gaaatttatc ggacgcaaaa aggcggtttc
     3961 ccgggtgctt tgctgcgggt tttacccggc aggctccgta ttcgcgccca atccgcccgg
     4021 agcggtgcgg gcaagggatg ttatgtttaa ttaaacactc atcatgagag ctagctaatt
     4081 ttttaaaaat taatcataac tttcattgct ttttcatcag cggcatgttt aaaaacatca
     4141 taggcttttt ctatatcgtt aaaattaaaa tgatgagtgg cgagtttttc tgttgctagt
     4201 tttcctgagc agcaggtttt cattaacatt cctgttgtat ttgcattaac gagacctgtt
     4261 gttattgtca ggttttttat ccacaacttc tcgatagcaa aactcacagg gttcccatga
     4321 acaccaacgt ttgctaaatg cccgccttct tttataatat tttgacaaat attccatgtt
     4381 ggttcgattc caactgcttc gattgcacaa tcgacaccac gctggtttgt caattccata
     4441 accctattaa tagcgttttc ttttgcagag ttaatagtat gagttgcccc catatcttta
     4501 gccattgcca aacggttttc atccatatcg atcatgatca actgcgatgg ggaataaagc
     4561 atgccggtga gtaggcaact cattccaaca ggacccgccc caacaatagc aacagtgtca
     4621 ccaggcttta ccattccatt ctggatccct atctcgtggg ctgtaggtag tgcatcagaa
     4681 agtaaaactc cggcatcgtc atttaattca tcagggaata agtaaagaga gttgtctgca
     4741 tatggtgttc tgacgaactc agcttgagtt ccatcaatca tataacccat aatccaacca
     4801 ccatcatttc tacaatgcgc atataattgt ttttgacaat tatcgcaggt gccgcaacga
     4861 ctaacgcaag aaataattac cttatcacct attttaaaat ttttaactgc tgagccaatt
     4921 tcctctatta taccaactcc ctcatgtccg agtattctcc cattaaaaga tcctgttttt
     4981 tcttttgcat ttttctctat ttctgggttc tttcctttta atattccaag atctgttcca
     5041 caaattgtgg ttttaatcat gcgaataata gcgtcgcctg gattgatta
//
