LOCUS       CP067975                5710 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3182 plasmid pC236_3, complete
            sequence.
ACCESSION   CP067975
VERSION     CP067975.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868956
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C236
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 59x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2021 12:18:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,329
            CDSs (total)                      :: 5,207
            Genes (coding)                    :: 5,111
            CDSs (with protein)               :: 5,111
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 44 of 96
            Pseudo Genes (incomplete)         :: 45 of 96
            Pseudo Genes (internal stop)      :: 16 of 96
            Pseudo Genes (multiple problems)  :: 9 of 96
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3182"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC236_3"
                     /geo_loc_name="USA: South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            1..192
                     /locus_tag="JJ433_26615"
     CDS             1..192
                     /locus_tag="JJ433_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMK08980.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLNASQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            192..593
                     /locus_tag="JJ433_26620"
     CDS             192..593
                     /locus_tag="JJ433_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="UMK08981.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHLSGQVSEQRDTASADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="DNA-binding protein"
     gene            complement(1331..1576)
                     /locus_tag="JJ433_26625"
     CDS             complement(1331..1576)
                     /locus_tag="JJ433_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMK08982.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKSLPKAVKQQVRERV
                     LRAIQDERESRLAKKMRREKYEAAEDTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1904..3590
                     /locus_tag="JJ433_26630"
                     /pseudo=""
     CDS             1904..3590
                     /locus_tag="JJ433_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /name="cloacin"
     gene            3600..3857
                     /locus_tag="JJ433_26635"
     CDS             3600..3857
                     /locus_tag="JJ433_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="UMK08978.1"
                     /translation="MGLKLDLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGCFDVKNEWVSLLQPYFKHKINLSDSSYFVSFDYRDGNW"
                     /name="cloacin"
     gene            3942..4091
                     /locus_tag="JJ433_26640"
     CDS             3942..4091
                     /locus_tag="JJ433_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UMK08979.1"
                     /translation="MKKVKAIFLFILIVSGFLIVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5081..5308
                     /locus_tag="JJ433_26645"
     CDS             5081..5308
                     /locus_tag="JJ433_26645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMK08983.1"
                     /translation="MPSEHDEQRDATDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     RGSVIAFSLARVRGNTPVLHFISLFSCNYR"
                     /name="hypothetical protein"
     BGC             1..5710
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4873..5088
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.258"
                     /coverage="100.0"
                     /evalue="7.539999999999999e-48"
     Typing          4327..4512
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="6.160000000000001e-73"
ORIGIN
        1 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg
       61 ctcctgctgg agaagcttaa cgagcttgat ctcgatgttg aggcggacct ttgtgagaaa
      121 ctccacgatg atgcggaaca tcttttccgt acactgtcct cgagactgaa tgcatcacag
      181 gatggtaact gatggccaga gtatctatta gtgaggctgc cagactgacg ggtaagagta
      241 gaacaacgct gcacaggtta attaaagcag gtgacttgtc cacctgtagc ggtgaacgta
      301 atgcaaaaat ggtagatact tcagagcttt tgcgtgtctt cggtcccttt gagcaaccta
      361 agggtgaaca cctttctgga caggtctctg aacagcgtga tacagcctct gctgaccaga
      421 atgaacaggt gattcgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt
      481 ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg
      541 caaaattaac tgaatccccg gcgccatggt ggaaattctg gaagaaatcc tgatcctgcc
      601 atggttgagt tttcctctcc ggccattccg ggagttcctg aaccttccca gatatcgcca
      661 gatccgggaa cactcctcac cacgaaaaat acccacacac cacgcaaaat taaaattttg
      721 cagataactc cttattatca gttggttagg ttttattaaa ttacgctgta ttaccttctg
      781 taaggtaaat ccaagtcgtc ggcgattcgc gtacgacttg ccgacgactc gtcatagagt
      841 catcgacgac tcactccata ctatcagtaa cttatgacga cttgactcta gagttgagtc
      901 gttcatttgg tgttttttga ccttggtgac tctagagtca agtcacgagt cgaatcgccg
      961 gtgaatcgtg tgctaagtcg tccggtgact tgccatcacg tcattgccgg ctgtcgctga
     1021 accggattcc tgaacgtgct gaaaacgggt tttccggctg cgccggcaac gcctcacggc
     1081 cacgcaataa attgcgtccc cgttcaccgt tttcgggtaa gaagaacatc ggaaacagga
     1141 cttaccccga ctgaatggcg tgaatttccg cgctatgcac ttgcgcgcat actcatgcat
     1201 gccgtaaaaa cagagcctgc gcgtttctgg cgggttttcg ggtggtttgt tgcctgtttt
     1261 gccggttttc cgtcagaaac gcgctgaggg tgcctgaggc ggtgcgggca acggatgtta
     1321 tggtaaatcg tcaacgccgg cgctgcggct gccaggtgaa ggtgtcttcc gccgcctcat
     1381 acttctcccg gcgcattttc ttcgccagcc tggattcacg ttcgtcctga atggcgcgaa
     1441 gcaccctttc ccgcacctgc tgttttaccg cctttggcag tgatttattc cctttcaggc
     1501 ccatggccag cgcctgggcg acaaaggcgt ccgccagacg cttctcttca gcgggggtga
     1561 gtttcaggtg tgtcatggtg gcctccgggt gtctctgggt tcctgaacag cttaattggt
     1621 agtccctctc ctcattccag tccgctttca gatatgcatg tacttacata aaagcaagtg
     1681 tgatgaatat cgcaaataat gaaatttgtt gccgggggaa actttcggga aaagacgcaa
     1741 cctgctgaca ggcggctctg gccgcttttt tgtgcctgaa gatgagaact aatctcatat
     1801 attgacacct gaaaacagca agggtaaggt aataattata ctgttcatat atacagtata
     1861 ttggttttaa tttattgttt taaatgtcaa agaggataac gatatgagtg gcggagacgg
     1921 acgaggtccg ggtaattcag gtctgggaca caatggtggt caggccagtg gaaatgtgaa
     1981 cggtacgtca ggtaaaggtg gcccttcatc tggtggtggt acggatccaa acagcgggcc
     2041 gggctggggt acgacgcata cacctaacgg ggatattcat aactataacc cgggtgagtt
     2101 tggtaacggc gggagtaaac ccggtggtaa tggcggtaac agcggcaatc atagcgggag
     2161 ctctggtggc ggacagtctt cagccaccgc gatggccttc ggtctgccag cccttgccac
     2221 tccgggggct gagggactgg ctttatccgt ctctggcgat gcgttgtcgg ccgctgttgc
     2281 tgatgtgctg gccgccctga aaggtccgtt taagtttggt ctgtgggggg atagcgatct
     2341 acggtgtgct gccttctgag attgcaaaag atgatccgaa catgatgtca aaaattatga
     2401 cgtcattacc ggccgatacg gtgacggaga ctccggtaag tactttacca ctggagcagg
     2461 cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg
     2521 cggttgtcgc gggtcggcca atgagtgttc ctgtggtgga tgcgaaaccg acaaaacgtc
     2581 cgggggtatt cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc gtaccgaaag
     2641 gtgttccggc cgcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc
     2701 cggctggttt tacggccggt ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga
     2761 ccggacagaa gccggtttat gtgtcggtga cagatgttct taccccggca caggtaaagc
     2821 agcgtcagga ggaagaaaag cgtcgtcagc aggcatggga cgccgctcac ccggaagagg
     2881 ggctgaaaga gagtatgata aagcgaaagc tgagctggat gccgaagata aaaatattgc
     2941 gaccttaaac ggtaggatta catcgacaga aaaaaccatt cctggtgcaa gaactgctgt
     3001 tcaggaagcc gataaaaagg tgaaagaagc cgaggcaaat aaagatgatt ttgtgactta
     3061 caacccgcct catgaatatg gctccgggtg gcaggaccag gttcgctatc ttgataagga
     3121 tattcagaat cagaatgaga aattaaaagc agctcaggca tctttaaacg caatgaatga
     3181 atccttatcc agagataagg ctgcgctttc cggggcgatg gagagccgga aacaaaagga
     3241 gaaaaaagcg aaggatgcag aaaataaatt aaatgaggaa aagaaaaaac ctcgcaaggg
     3301 aactaaagat tacggccatg attatcatcc agccccgaaa actgaagaca taaagggggc
     3361 taggtgacct caaaaaaggt acacctaaaa caccaatgca gggaggtgga ggtaggcgta
     3421 aacgctgggt tggtgataaa ggtcgtaaga tttatgaatg ggattcccag cacggtgagc
     3481 ttgaagggta ccgcgccagt gatggcgaac acctcggcgc attcgatcca aaaacgggta
     3541 agcaggttaa agggccggat ccaaaaagaa acattaaaaa atatctttga gaggtaagta
     3601 tgggacttaa attagattta acctggtttg ataagaaaac tgaagagttt aaaggggaag
     3661 agtattctaa agactttggt gatgatggtt ctgtcattga aagtctgggg atgcctttaa
     3721 aggataatat taacaatggt tgttttgatg tgaaaaatga gtgggtttca ttattgcaac
     3781 cctactttaa acataaaatc aatctttctg atagttcata ttttgtttca tttgattatc
     3841 gggatggcaa ctggtaataa aaatttatca aggatgagtt gaaatacaga cttccggtat
     3901 tcagggatga atgctggagt ctttaaatac agggaaagta tatgaaaaag gtaaaggcta
     3961 tttttctttt tatattgata gtttctggct ttcttattgt cgcatgtcag gcaaactata
     4021 tcagggatgt tcagggcggg acggtggcac catcatcctc ctctgagctg acgggaattg
     4081 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttctgcgc gtaatctgct
     4141 gcctgtaaac gaaaaaacca cccggcaagg tggtttttcg aaggttcagc aaaccggcaa
     4201 gtttctgaac cgtggtaaca gtcttgtgcg agacatgtca ccaaatctgt ccttccagtg
     4261 tagccgcagt caggccacca cttcaagaac tcttcgaaac atatctcgca catttcagtt
     4321 accagtggct gctgccagtg gcgatttatc gtgtctttcc gggttggacc caagagaaca
     4381 gttaccggaa caggcgcagc agtagggctg aaaggggggt tcctgcatac agcccggccc
     4441 ggagcgaact gcctaagttg gtccgagata ccagaagacg tgagctatga gaaagcgcca
     4501 cgtgtcccgt aagggagaaa ggcggaaagg tatccggtaa ccgatatggc gggaacgtgg
     4561 gggcgcagga gggagcgatt caccggaaac ggtgatgatc tttaagccac gtcgggtttc
     4621 gtcaccgctg gcggattcat ggttgagcct tacggctccc acagatgcac aaaaaagtgt
     4681 ctgttatgtg aactccgtca gggggcggag cctatggaaa aagcgcctgc ggcgcggcct
     4741 tttcgtcctt tcttttttgc gcacatgttc tgtgatgcgt tatcccctga ttctgtggat
     4801 aaggtatgag tctgttgtgt gagcgtaggc aacgcccgcc gcagccgaac ggccggagcg
     4861 tgagcgaccg agtgagcgag gaggcgtcat ttccccggaa ccggcattca gcacttacgc
     4921 gttccggcca catttttctc ctgccacatg aagcacttcc ctggtaacca cgctcatgcc
     4981 aacataggaa gccagtacac actccgctag cgctacgtga ctggttcagg gctgcgcccc
     5041 gaaacccgct aacaacctct cagctactgg atatcacccc atgccatctg aacatgatga
     5101 acaacgggat gctactgata cgcagcttcc cggcgaatat gacctttttg atttaatggc
     5161 gcaggttact gaagagaacc tgcatgcccc tgtggattct gggccgcctg tcggccgggg
     5221 aagcgttata gctttttcgc ttgcccgcgt acgtggtaat acgccggtat tacattttat
     5281 ttctttattt tcatgtaatt acaggtgatt gtaatacgtt gcgtattaca taaataaacg
     5341 gagatttcag tttttacctt ttaattcatg cagttgcgat gaaaatcgcg gctgcgttag
     5401 gtgtatgacc atttaagggg taaaaaatta tcattctggc cgcgtgatct gcgcaggtta
     5461 aggagcatcg gcgacgaaaa agcgtcttta actgcttcgt tcatgcttgc atgaacggat
     5521 gatttttcca gactacaggt aaactacagg aagactacaa aagttgatga tttaatactg
     5581 atttttagtg cgatgactac agataaacta caaaataact acaggattac ctcagatccg
     5641 ttctctgaac cattcgtaca tccgaccccc ttaccggttt acactgagca ctgatgctct
     5701 gagggccctt
//
