LOCUS       CP067971                5631 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3183 plasmid pC237_3, complete
            sequence.
ACCESSION   CP067971
VERSION     CP067971.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868957
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C237
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 221x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2021 12:23:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,328
            CDSs (total)                      :: 5,203
            Genes (coding)                    :: 5,114
            CDSs (with protein)               :: 5,114
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 36 of 89
            Pseudo Genes (incomplete)         :: 44 of 89
            Pseudo Genes (internal stop)      :: 16 of 89
            Pseudo Genes (multiple problems)  :: 7 of 89
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3183"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC237_3"
                     /geo_loc_name="USA: South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            1..192
                     /locus_tag="JJ434_26610"
     CDS             1..192
                     /locus_tag="JJ434_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMK03870.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1395..2261)
                     /locus_tag="JJ434_26615"
     CDS             complement(1395..2261)
                     /locus_tag="JJ434_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="UMK03871.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(2263..3315)
                     /locus_tag="JJ434_26620"
     CDS             complement(2263..3315)
                     /locus_tag="JJ434_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMK03872.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            complement(3796..4029)
                     /locus_tag="JJ434_26625"
     CDS             complement(3796..4029)
                     /locus_tag="JJ434_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UMK03867.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4036..4566)
                     /locus_tag="JJ434_26630"
     CDS             complement(4036..4566)
                     /locus_tag="JJ434_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UMK03868.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(4544..5302)
                     /locus_tag="JJ434_26635"
                     /pseudo=""
     CDS             complement(4544..5302)
                     /locus_tag="JJ434_26635"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5244..5567)
                     /locus_tag="JJ434_26640"
     CDS             complement(5244..5567)
                     /locus_tag="JJ434_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UMK03869.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(287..368)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          complement(825..1012)
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          complement(3693..5254)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaccaaac aggaaaaaac cgcccttaac atggcccgct tcatcagagg ccagacatta
       61 acgctgctgg agaagctcaa cgaactggac gcggatgaac aggcagacat ctgtgaatcg
      121 cttcacgacc acgccgatga gctttaccgc agttgcctcg cacgcttcgg ggatgacggt
      181 gaaaaccgct gacacatgca gctcccggag acggtcacag cttgtctgtg agcggatgcc
      241 gggagcagac aagcccgcca gggcgcgtca ggccgttgac ctttggggtg tcggggcgaa
      301 gccctgaccc agtcacgtag cgatagcgga gtgtatactg gcttaaccat tcggcatcag
      361 tgcggattgt atgaaaagtg cgccatgtcc ggtgtgaaat gccgcacaga tgcgtaagga
      421 gaaaatgcca gttcaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg
      481 ttcggctgcg gcgagcggac tctcctttct caaaaacgct aaccctgtta tccacagaac
      541 ccggggataa accaggaaag aacatgtgcg caaaaaacga aaccccggga aaggccgcgc
      601 cggaggcgct ttttccatag gctccgcccc cctgacggac ttcacaaaac agacactttt
      661 tcggtgcatc tgtgggagcc ttgcggctca accatgaaat ccgccagcgg tggcgaaacc
      721 cgacgtggct taaagatcat caccgtttcc ggtgaatcgc tccctcctgc gcccccacgt
      781 tcccgccata tcgattaccg gatacctttt ccgcctttct cccttacggg acacgtggcg
      841 ctttctcata gctcacgtct tctggtatct cggggcgact taggcagttc gctccgggct
      901 gggctgtatg caggaacccc ccgttcagcc cgactgctgc gccttctccg gtaactgttc
      961 tcttgggtcc aacccggaaa agacaccaca aagcgccact ggcagcagcc attggtaaaa
     1021 gtcatgtgcg agatatttag agagtacttg aagtggctgc cggagtgcgg ctacactaga
     1081 agaacaattt tggtgactcg tctcgcataa gacagttacc tggttaagca gtctgcaagc
     1141 tgacttaacc ttcgatcaaa ccgccctgca aggcggtttt ttcgtttaca ggcagcagat
     1201 tacgcgcaga aaaaaaggat ctcaagagga tcctttgatc tttttgttac aaacgtctct
     1261 atttgactcc cctgagagca ttttagcgcg cctctgaccc ccttggttac attccgacac
     1321 cagaaacgct cccgtttttt acgtgcattt ggcacggtat cgatggaggc cggggggcaa
     1381 tcctgaacag aatgttactc ctctgagtat tttctgttaa gaaaatatac acttagcaca
     1441 gttacaataa atcctacccc ctgtaagaat gtgaattgtt catccaaaaa caaccatgac
     1501 aaaaataatg caactagcgg aataaagtac aacattatcc ctgccaaatg aactgggagc
     1561 caacgcaagg agttcaagta aaaacaaaaa gctaaaaaat attgtaatat tccagatgaa
     1621 aaaacaataa tcacggattc caacggaaga taaattgatg atttagatat aaaaataaag
     1681 agcgatgaca gtataaggga gaatgtcaac tgtccgctca ataatgtgat aggctcaaac
     1741 gatttaataa ggcgagacga tataacaaca taaaatgctg ccgataacac accggcaaca
     1801 acaagacaat atccaacaat attattcgat ccattactga tgtctggcaa ggctaccaaa
     1861 atactgccaa caattgcacc tataaggaac aatataaaat attgcgggga attaatttta
     1921 actgagcaaa taagaacaga caacaccaat ataaacactg attcaagtga aaaaataatt
     1981 gatgcgcttc ctgctgaaat aaaataaagt ccgtagagtg taaaagtata cgctaagaat
     2041 ggttcaaaaa cgcctgtcgc ataagcaagt atcgcttttg agtttaatgg gagttttttt
     2101 ctttttataa aaagaactat ccaagatagt attactgcac tacaaatctg ccagaaaaac
     2161 acccctccgg catctagttc ttcaactcct tttttgctca ttacgatagc tgccccccat
     2221 gagaacgctg ctaaaaaagc aaaaagcatt cctttaatca tattaatcat cctctttctc
     2281 ataaaggggt ttgttttcaa atttccttgg ttgaaaggag ttaaaaaaca tagatgcatc
     2341 atctacagga ataccaataa actctccata ataaggatca tctataggaa actcttctaa
     2401 tgaaacataa aacaatacat tccagttgaa gatcattgga tcactatttt ctatttcatg
     2461 gagcaatctt tcatccttag ttaattctcc tggaattaat acatcagtat gataaaaacc
     2521 gctgtaataa gaactattac cggtgtgcac tgggcatcca aaacgatcca aattagattc
     2581 tgaatgccat gttgtagaaa tcccttccgg atgtgaatac ccacccttac caacagactg
     2641 aatagtcatg ttacgaatat ccgctaacgc ttgttcttga tctttataga aacctttttc
     2701 tatcgccagc ctcataaaat gccccggggc atttacagaa ataagtattc cccctggtat
     2761 tcttctatca tgatttcttg ttctatgagc agaagagtaa aaaacattaa caccagcctt
     2821 atatattttt agcccatctt tatcctgaaa tggtacgctt tcagtataaa taacatcctc
     2881 ttttattgga tagcactctg gcatatgcag cgaacaaata tatttttgta tgtccactaa
     2941 ctcttctgat ggacgagcaa aggcaagttg tggatgaata aaataaatga gatgcgcact
     3001 gacctctcct cttcttgcgc gttctttaaa acgaactctt tcattacgca aaaaatcaaa
     3061 caaatgacca tcatcattat atagttgggg gctatttaca atagataaat ggaaatcaag
     3121 ccctttaatt ttcttgcttg ccaatttacc aaatacacag ggctgattgc ctgacaccca
     3181 ttttcttagt aaatcttcaa ttttatcttt agaagtttcg ttaaagataa atgaatgaat
     3241 attatcaata tcttttccaa atgaaaaact ggatgataat tgtggtagtt tatttatacg
     3301 ttgttgaaga ttcatgaaag ctcccacaag acacctcata tattttttta tatactgaaa
     3361 ctacattata tttattaccg gcgtaaaacg aaataattta ttatcgttac cgatatttta
     3421 aaaaaatcat tatttgcaca gtgttcgagt tgttagtatc actccttcct gaaaatccgc
     3481 tccatttacc ataacgtcgc attgtacgca ccgccaaaac gcgttcggcg cgatcacggc
     3541 agcagacagg taaaaatggc aacaaaccac ccgaaaaact gccgcgatcg cacctgataa
     3601 attttaaccg catgaatacc tatgcaacca gatgctacag gccacattag cccgccttaa
     3661 tccactgaac acgtcatttt tcatggtttc accatcccag cgaagggcca tccagtgtcc
     3721 gctcctggat ttccggctga cgctcccgct ccagagataa ctcatgttcg cgttcctgca
     3781 tcagccgttc atcccttatc tcctgttctc gctgtataac tggctcaagc gttctgtccg
     3841 ctcgctcaag tgctgcacct gctgactcaa ctgcatgact cgcccgttca gcatcgcgtt
     3901 gtcccgttgc gtaagcgaaa acattttctg caattccacg aaggcgttct cccattcgtt
     3961 cagccgctgc atatagtcct gttgcagttg ctctaatgcg ttcagcaaat gtctttccag
     4021 ctccgtcatg ctctgtcact cctgcaccag tctcactgcg ttcagtttgc cgttgtcctt
     4081 cgtccagttc gtttcggctt tcatcccctt cggcaacacc agaaaacgtc cgttgctgtc
     4141 ctcgtgataa cgcacgcccc atgtcttcgc gttcagcttc tccagactct ctttctgctc
     4201 gcggagaata ttcatattcg cgtttatctt catgccctgg taccacagca gactcccggt
     4261 cgtggcaaac atcagtactg agacgataat cagccacagc cagcgtttca cgctcatttt
     4321 cagtacggtt ttccagtgcg cgtccatcgt atcgacggtc gttttctgat acctctccag
     4381 cagttctttc agggctgttg tatggtcgtt gatagcgttc ctgattaaag attcgtttga
     4441 ggtcagttcc ctgtttacag attcttcgag cttcctgaac tcgtcgttca gcattttctc
     4501 tgtagattcg gctctctctt tcagcttttt ctcgaactcc gcgcccgtct gtaaaagatt
     4561 gctcataaaa cgctcctttc agtctgatat tcctcccgcc gttcgggtct gcaatgctga
     4621 tactgcttcg cgtcaccctg accacttcaa gccccgcctc tgtgagcgcc tgaatcacat
     4681 cctgacggcc ttttatctct ccggcatggt aaagcgcgtc tatcccgcgt gtgacgccct
     4741 cagcaagcgc ctgtttcgtt tccggcaggt tatcaggaag ggtcagcgtc cggcggttct
     4801 ccggggcgtt ggggtcatgc agaccgtaat ggtgattcac cagcgtctgc caggcatcaa
     4861 ttctcggcct gtctgcccgg tcgtagtacg gctggaggcg ttttccggtc tgtagctcca
     4921 tgttcggaat gacaaaattc agctcaagcc gtcccttgtc ctggtgctcc acccacagga
     4981 tgctgtactg atttttctcg agaccgggca tcagtacacg ctcaaagctc gccatcactt
     5041 tttcacgccc tccaggcggc agctccttct ccgcgaacga cagaacacct gacgtgtatt
     5101 tcttcgcaaa tggcgtggca tcgatgagtt cccggacctc ttccggattc ccctgaagca
     5161 ccgttgcccc ttcccggtta cgctcccttc ccagcaggta atcaaccgga ccactgccac
     5221 caccttttcc cctggcatga aacttaacta tcatcccgcg ccccctgttc cctgaccgcc
     5281 agacgcagcc ggcgcagctc atccccgatg gccatcagtg cggccaccac ctgaacccgg
     5341 tcaccggaag accactgccc gctgttcacc ttcctcgctg tctggttaag gttattcccg
     5401 atggcggcca gctgacgcag taacggcggt gccagtgtcg gcagcttccc ggaacgggca
     5461 accggctcac ccaggcagac ccgccgcatc cataccgcca gttgtttacc ctcacagcgt
     5521 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctcgcgtttc
     5581 atcggtatca ttacccccat gaacagaacc cccccttaca cggaggcatc a
//
