LOCUS       CP067881                5714 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3206 plasmid pC260_4, complete
            sequence.
ACCESSION   CP067881
VERSION     CP067881.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868976
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C260
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 229x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 16:07:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,632
            CDSs (total)                      :: 5,508
            Genes (coding)                    :: 5,413
            CDSs (with protein)               :: 5,413
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 95
            CDSs (without protein)            :: 95
            Pseudo Genes (ambiguous residues) :: 0 of 95
            Pseudo Genes (frameshifted)       :: 42 of 95
            Pseudo Genes (incomplete)         :: 46 of 95
            Pseudo Genes (internal stop)      :: 17 of 95
            Pseudo Genes (multiple problems)  :: 10 of 95
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3206"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC260_4"
                     /geo_loc_name="USA: South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            1..192
                     /locus_tag="JJ453_28130"
     CDS             1..192
                     /locus_tag="JJ453_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMI66333.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLNASQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            192..593
                     /locus_tag="JJ453_28135"
     CDS             192..593
                     /locus_tag="JJ453_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="UMI66334.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHLSGQVSEQRDTASADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="DNA-binding protein"
     gene            complement(1333..1578)
                     /locus_tag="JJ453_28140"
     CDS             complement(1333..1578)
                     /locus_tag="JJ453_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI66335.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKSLPKAVKQQVRERV
                     LRAIQDERESRLAKKMRREKYEAAEDTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1905..3590
                     /locus_tag="JJ453_28145"
     CDS             1905..3590
                     /locus_tag="JJ453_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="UMI66336.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPNMMSK
                     IMTSLPADTVTETPVSTLPLEQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNGRITSTEKTIPGARTAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYHPAPKTEDIKGLGDLKKGTPKTPMQGGGGRRKRWVGDKGRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            3600..3857
                     /locus_tag="JJ453_28150"
     CDS             3600..3857
                     /locus_tag="JJ453_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="UMI66330.1"
                     /translation="MGLKLDLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGCFDVKNEWVSLLQPYFKHKINLSDSSYFVSFDYRDGNW"
                     /name="cloacin"
     gene            3942..4091
                     /locus_tag="JJ453_28155"
     CDS             3942..4091
                     /locus_tag="JJ453_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UMI66331.1"
                     /translation="MKKVKAIFLFILIVSGFLIVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5086..5235
                     /locus_tag="JJ453_28160"
     CDS             5086..5235
                     /locus_tag="JJ453_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI66332.1"
                     /translation="MPSEHDEQRDATDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     BGC             1..5714
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4878..5093
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="83.258"
                     /coverage="100.0"
                     /evalue="7.539999999999999e-48"
     Typing          4329..4514
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="6.160000000000001e-73"
ORIGIN
        1 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg
       61 ctcctgctgg agaagcttaa cgagcttgat ctcgatgttg aggcggacct ttgtgagaaa
      121 ctccacgatg atgcggaaca tcttttccgt acactgtcct cgagactgaa tgcatcacag
      181 gatggtaact gatggccaga gtatctatta gtgaggctgc cagactgacg ggtaagagta
      241 gaacaacgct gcacaggtta attaaagcag gtgacttgtc cacctgtagc ggtgaacgta
      301 atgcaaaaat ggtagatact tcagagcttt tgcgtgtctt cggtcccttt gagcaaccta
      361 agggtgaaca cctttctgga caggtctctg aacagcgtga tacagcctct gctgaccaga
      421 atgaacaggt gattcgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt
      481 ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg
      541 caaaattaac tgaatccccg gcgccatggt ggaaattctg gaagaaatcc tgatcctgcc
      601 atggttgagt tttcctctcc ggccattccg gggagttcct gaaccttccc agatatcgcc
      661 agatccggga acactcctca ccacgaaaaa tacccacaca ccacgcaaaa ttaaaatttt
      721 gcagataact ccttattatc agttggttag gttttattaa attacgctgt attaccttct
      781 gtaaggtaaa tccaagtcgt cggcgattcg cgtacgactt gccgacgact cgtcatagag
      841 tcatcgacga ctcactccat actatcagta acttatgacg acttgactct agagttgagt
      901 cgttcatttg gtgttttttt gaccttggtg actctagagt caagtcacga gtcgaatcgc
      961 cggtgaatcg tgtgctaagt cgtccggtga cttgccatca cgtcattgcc ggctgtcgct
     1021 gaaccggatt cctgaacgtg ctgaaaacgg gttttccggc tgcgccggca acgcctcacg
     1081 gccacgcaat aaattgcgtc cccgttcacc gttttcgggt aagaagaaca tcggaaacag
     1141 gacttacccc gactgaatgg cgtgaatttc cgcgctatgc acttgcgcgc atactcatgc
     1201 atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt
     1261 ttgccggttt tccgtcagaa acgcgctgag ggtgcctgag gcggtgcggg caacggatgt
     1321 tatggtaaat cgtcaacgcc ggcgctgcgg ctgccaggtg aaggtgtctt ccgccgcctc
     1381 atacttctcc cggcgcattt tcttcgccag cctggattca cgttcgtcct gaatggcgcg
     1441 aagcaccctt tcccgcacct gctgttttac cgcctttggc agtgatttat tccctttcag
     1501 gcccatggcc agcgcctggg cgacaaaggc gtccgccaga cgcttctctt cagcgggggt
     1561 gagtttcagg tgtgtcatgg tggcctccgg gtgtctctgg gttcctgaac agcttaattg
     1621 gtagtcctct cctcattcca gtccgctttc agatatgcat gtacttacat aaaagcaagt
     1681 gtgatgaata tcgcaaataa tgaaatttgt tgccggggga aactttcggg aaaagacgca
     1741 acctgctgac aggcggctct ggccgctttt ttgtgcctga agatgagaac taatctcata
     1801 tattgacacc tgaaaacagc aagggtaagg taataattat actgttcata tatacagtat
     1861 attggtttta atttattgtt ttaaatgtca aagaggataa cgatatgagt ggcggagacg
     1921 gacgaggtcc gggtaattca ggtctgggac acaatggtgg tcaggccagt ggaaatgtga
     1981 acggtacgtc aggtaaaggt ggcccttcat ctggtggtgg tacggatcca aacagcgggc
     2041 cgggctgggg tacgacgcat acacctaacg gggatattca taactataac ccgggtgagt
     2101 ttggtaacgg cgggagtaaa cccggtggta atggcggtaa cagcggcaat catagcggga
     2161 gctctggtgg cggacagtct tcagccaccg cgatggcctt cggtctgcca gcccttgcca
     2221 ctccgggggc tgagggactg gctttatccg tctctggcga tgcgttgtcg gccgctgttg
     2281 ctgatgtgct ggccgccctg aaaggtccgt ttaagtttgg tctgtggggg atagcgatct
     2341 acggtgtgct gccttctgag attgcaaaag atgatccgaa catgatgtca aaaattatga
     2401 cgtcattacc ggccgatacg gtgacggaga ctccggtaag tactttacca ctggagcagg
     2461 cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg
     2521 cggttgtcgc gggtcggcca atgagtgttc ctgtggtgga tgcgaaaccg acaaaacgtc
     2581 cgggggtatt cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc gtaccgaaag
     2641 gtgttccggc cgcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc
     2701 cggctggttt tacggccggt ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga
     2761 ccggacagaa gccggtttat gtgtcggtga cagatgttct taccccggca caggtaaagc
     2821 agcgtcagga ggaagaaaag cgtcgtcagc aggcatggga cgccgctcac ccggaagagg
     2881 ggctgaaaag agagtatgat aaagcgaaag ctgagctgga tgccgaagat aaaaatattg
     2941 cgaccttaaa cggtaggatt acatcgacag aaaaaaccat tcctggtgca agaactgctg
     3001 ttcaggaagc cgataaaaag gtgaaagaag ccgaggcaaa taaagatgat tttgtgactt
     3061 acaacccgcc tcatgaatat ggctccgggt ggcaggacca ggttcgctat cttgataagg
     3121 atattcagaa tcagaatgag aaattaaaag cagctcaggc atctttaaac gcaatgaatg
     3181 aatccttatc cagagataag gctgcgcttt ccggggcgat ggagagccgg aaacaaaagg
     3241 agaaaaaagc gaaggatgca gaaaataaat taaatgagga aaagaaaaaa cctcgcaagg
     3301 gaactaaaga ttacggccat gattatcatc cagccccgaa aactgaagac ataaaggggc
     3361 taggtgacct caaaaaaggt acacctaaaa caccaatgca gggaggtgga ggtaggcgta
     3421 aacgctgggt tggtgataaa ggtcgtaaga tttatgaatg ggattcccag cacggtgagc
     3481 ttgaagggta ccgcgccagt gatggcgaac acctcggcgc attcgatcca aaaacgggta
     3541 agcaggttaa agggccggat ccaaaaagaa acattaaaaa atatctttga gaggtaagta
     3601 tgggacttaa attagattta acctggtttg ataagaaaac tgaagagttt aaaggggaag
     3661 agtattctaa agactttggt gatgatggtt ctgtcattga aagtctgggg atgcctttaa
     3721 aggataatat taacaatggt tgttttgatg tgaaaaatga gtgggtttca ttattgcaac
     3781 cctactttaa acataaaatc aatctttctg atagttcata ttttgtttca tttgattatc
     3841 gggatggcaa ctggtaataa aaatttatca aggatgagtt gaaatacaga cttccggtat
     3901 tcagggatga atgctggagt ctttaaatac agggaaagta tatgaaaaag gtaaaggcta
     3961 tttttctttt tatattgata gtttctggct ttcttattgt cgcatgtcag gcaaactata
     4021 tcagggatgt tcagggcggg acggtggcac catcatcctc ctctgagctg acgggaattg
     4081 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4141 ctgcctgtaa acgaaaaaac cacccggcaa ggtggttttt cgaaggttca gcaaaccggc
     4201 aagtttctga accgtggtaa cagtcttgtg cgagacatgt caccaaatct gtccttccag
     4261 tgtagccgca gtcaggccac cacttcaaga actcttcgaa acatatctcg cacatttcag
     4321 ttaccagtgg ctgctgccag tggcgattta tcgtgtcttt ccgggttgga cccaagagaa
     4381 cagttaccgg aacaggcgca gcagtagggc tgaaaggggg gttcctgcat acagcccggc
     4441 ccggagcgaa ctgcctaagt tggtccgaga taccagaaga cgtgagctat gagaaagcgc
     4501 cacgtgtccc gtaagggaga aaggcggaaa ggtatccggt aaccgatatg gcgggaacgt
     4561 gggggcgcag gagggagcga ttcaccggaa acggtgatga tctttaagcc acgtcgggtt
     4621 tcgtcaccgc tggcggattc atggttgagc cttacggctc ccacagatgc acaaaaaaag
     4681 tgtctgttat gtgaactccg tcaggggggc ggagcctatg gaaaaagcgc ctgcggcgcg
     4741 gccttttcgt cctttctttt tttgcgcaca tgttctgtga tgcgttatcc cctgattctg
     4801 tggataaggt atgagtctgt tgtgtgagcg taggcaacgc ccgccgcagc cgaacggccg
     4861 gagcgtgagc gaccgagtga gcgaggaggc gtcatttccc cggaaccggc attcagcact
     4921 tacgcgttcc ggccacattt ttctcctgcc acatgaagca cttccctggt aaccacgctc
     4981 atgccaacat aggaagccag tacacactcc gctagcgcta cgtgactggt tcagggctgc
     5041 gccccgaaac ccgctaacaa cctctcagct actggatatc accccatgcc atctgaacat
     5101 gatgaacaac gggatgctac tgatacgcag cttcccggcg aatatgacct ttttgattta
     5161 atggcgcagg ttactgaaga gaacctgcat gcccctgtgg attctgggcc gcctgtcggc
     5221 cgggaagcgt tatagctttt tcgcttgccc gcgtacgtgg taatacgccg gtattacatt
     5281 ttatttcttt attttcatgt aattacaggt gattgtaata cgttgcgtat tacataaata
     5341 aacggagatt tcagttttta ccttttaatt catgcagttg cgatgaaaat cgcggctgcg
     5401 ttaggtgtat gaccatttaa ggggtaaaaa attatcattc tggccgcgtg atctgcgcag
     5461 gttaaggagc atcggcgacg aaaaagcgtc tttaactgct tcgttcatgc ttgcatgaac
     5521 ggatgatttt tccagactac aggtaaacta caggaagact acaaaagttg atgatttaat
     5581 actgattttt agtgcgatga ctacagataa actacaaaat aactacagga ttacctcaga
     5641 tccgttctct gaaccattcg tacatccgac ccccttaccg gtttacactg agcactgatg
     5701 ctctgagggc cctt
//
