LOCUS       CP067842                2058 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3223 plasmid pC277_6, complete
            sequence.
ACCESSION   CP067842
VERSION     CP067842.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868986
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C277
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 186x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:48:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,476
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 52 of 120
            Pseudo Genes (incomplete)         :: 60 of 120
            Pseudo Genes (internal stop)      :: 29 of 120
            Pseudo Genes (multiple problems)  :: 19 of 120
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3223"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC277_6"
                     /geo_loc_name="USA: South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            join(1789..2058,1..720)
                     /locus_tag="JJ463_27375"
     CDS             join(1789..2058,1..720)
                     /locus_tag="JJ463_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UMI17348.1"
                     /translation="MQNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIEELGETLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPKWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVKKYRRSPTKDKTESD"
                     /name="protein rep"
     gene            1274..1465
                     /locus_tag="JJ463_27380"
     CDS             1274..1465
                     /locus_tag="JJ463_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17347.1"
                     /translation="MIVRLDNRLFEILDEMGAHVDREMFMAAYAQRKAEKENDPRHIRQ
                     RKLTRLAHDLMSESDPEL"
                     /name="hypothetical protein"
     Typing          complement(447..638)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 atgtggcaag cccgtttcta ccagtccctg ccgcgtatcg tggcggatta ccccgattcc
       61 cgctggatgt ttctgaccct gaccgtgcgc aactgcgcga tcgaggagct gggcgagacg
      121 ctgagccgga tgaacacggc tttccagcga ctgaaggacc gtaaagagtt ccgaccagtt
      181 cagggctgga tacgcaccac ggaagtcacc cgcggcagtg acggcagcgc acacccgcac
      241 ttccacaccc tgatgatggt tccgccgggc atgcttaacg ggaagagtta cgtcaggcat
      301 gaacgctggg ttgagctgtg gcgtgagtgc ctgcgggtca gttacgatcc gaatgtcgat
      361 gtccgggcag tgaaaccccg caagccgaag gacggcgaat cgctggcctg cgcgaccgcc
      421 gaactggtgc gcggcgcagt ggccgaaacc ctgaaatatt ccacgaagcc ggccgacatg
      481 gtggccgatc ctaaatggtt cctcgaactg acccgccaga cgcacaagcg ccgctttgtg
      541 gcgaccggcg gcgcactcaa agacgtgctg aaactcgatc aggaaaccga cgccgacatg
      601 gtgatcgggg atgatatttc cgagggcgat gatgacggtt cccgcatcgc gttcgagtgg
      661 aagaccgagg tcaaaaagta ccggcgttcg ccgacgaaag ataaaaccga atcagactaa
      721 cagggtgtcg gggctgcgcc ctgaccatga gggctttggg catccgtcac cttggtggcg
      781 gtgtaccaaa gtcacatctt gtccgattga ttttactccg atgcaggata gggctaacgc
      841 cgcaaagtga cgtcactcga ttactgagtt cggattccga acctgggttt ttcttccggt
      901 aattttccgg aaggcgcgct tagtagtttc tcatctggcg atgagaaacg cgcggtgggg
      961 caagcccacg aaaaacaaaa actgataacg gacaagatgt gtttttggag taccgccatc
     1021 acgatgccgg taccaaaaac gatccgtcag ggctgcgccc cgacaccctg ccaacacctc
     1081 acgccgccag attaaaatct gtcagcgttc gccgttcccg aaatcgtcaa acgctaaatc
     1141 gcggggtccc gctggcttgc cagtgggttc cgatttagtg attgacgaca gcacccgtaa
     1201 aatacttaca gggggtggtt gtgatcacgc agtgagcgca accacctgat aagcgttttt
     1261 tgagggggta ggcatgatcg tcagactgga taaccggctg tttgagattc tcgatgagat
     1321 gggtgcgcat gtggatcggg aaatgttcat ggcggcctat gcgcagcgga aggccgaaaa
     1381 agaaaatgat ccccggcata tccgtcaacg gaaactgacc aggttggctc atgatttgat
     1441 gtcagaatcg gaccctgaat tgtgagcctc gcgtgcgcgc gagggaaatc ggtcagaaaa
     1501 tggccggagt ctcgtgcgcg cgatccttgt atttatactt aagggataac tctggcgtca
     1561 aaaaacgccg ccagcccttg tgccgcaagg gtttgagtgt gatttgcctg aaaatggttt
     1621 tggaagtatt ctgtcgggag tggtcgcaga aaaaagcgac ccccgcaact gcgctaacag
     1681 ttcgggggtg tgaccacctg taggaattcg aatttggaca cgacaacaca ggtgatatgg
     1741 caatgataac acgtcaaatg agccatcgcc cagcatgggg cggtgtgcat gcaaaatcct
     1801 gaactccaga acctaactga ctactccccc tcagatgcgc cgtgggacgc tcacagaagc
     1861 gtttctgacg acgtgggagg gatttacctg cttgcggctg aatatgagcg ctacggggcg
     1921 cgtatggcct cctgtggcgg tttactgagg tttgggtggt ctaccctcaa agaaaccgga
     1981 gaaacgcgcc tgaggctgcg tgaggcgcat ttctgccgtg tccggcactg tcctgtctgc
     2041 cagtggaggc gctccctg
//
