LOCUS       CP067841                5234 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3223 plasmid pC277_5, complete
            sequence.
ACCESSION   CP067841
VERSION     CP067841.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868986
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C277
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 186x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:48:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,476
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 52 of 120
            Pseudo Genes (incomplete)         :: 60 of 120
            Pseudo Genes (internal stop)      :: 29 of 120
            Pseudo Genes (multiple problems)  :: 19 of 120
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3223"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC277_5"
                     /geo_loc_name="USA: South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            join(4919..5234,1..11)
                     /gene="mobC"
                     /locus_tag="JJ463_27330"
     CDS             join(4919..5234,1..11)
                     /gene="mobC"
                     /locus_tag="JJ463_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="UMI17341.1"
                     /translation="MEKRTKEIKIRLTETEHQRLLDRCDRTHLAEWLRQLGLGEHTSRK
                     RRVPDVAPELLRQVSGIGNNLNQIARRLNQTDSLTPSERASLLVVLTSLDRQLGDLLEQ
                     NRDR"
                     /name="mobC"
     gene            join(5199..5234,1..702)
                     /locus_tag="JJ463_27335"
                     /pseudo=""
     CDS             join(5199..5234,1..702)
                     /locus_tag="JJ463_27335"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1174
                     /locus_tag="JJ463_27340"
     CDS             689..1174
                     /locus_tag="JJ463_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UMI17342.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDTELSVKREFARLETSISAELQSSS
                     QSISNAIREQNGRLTGMIRTSVSAAMAWIFLCFALLIGILGGIIWFQGTIITSRLSEMD
                     SYSQQLESLKAKSGAGVTIYSDDKNRNTYLVVLPKQAHGVETYTSTTDNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            1235..1468
                     /locus_tag="JJ463_27345"
     CDS             1235..1468
                     /locus_tag="JJ463_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UMI17343.1"
                     /translation="MTEMEKQLLSALESLQTSYEQQQQAWQDSYSSLQHMFETTSQALT
                     HSDRVCQHLSSQVESLRAQVESLSRNVSRLMR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1624..1956
                     /locus_tag="JJ463_27350"
     CDS             1624..1956
                     /locus_tag="JJ463_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UMI17344.1"
                     /translation="MSHAIEFIETPMFTRQIKQIATDEELRELQKTLIESPDKGDLIQK
                     TGGLRKVRMATGNQGKSGSARVIYFLATTEVIYLVMAYPKSTKDSLTDAEKAALKMLTQ
                     QLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1958..2242
                     /locus_tag="JJ463_27355"
     CDS             1958..2242
                     /locus_tag="JJ463_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UMI17345.1"
                     /translation="MSFFDELKASLEEAVEIKNGVREPARVTRYELADVKAIRAQLNVS
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFQELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            2436..3341
                     /locus_tag="JJ463_27360"
     CDS             2436..3341
                     /locus_tag="JJ463_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UMI17346.1"
                     /translation="MHNAALELFNDRLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPENGHAHLLYALKTSIRTAPDGKMK
                     PLCYAAAVENALRKKLEADTGYSGLICKNPNHGHWKVAVWQPELYTLDWLADFLDLNAA
                     NEKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERASAYNLQFAVPLD
                     ENEVSGIAKSIAKWTSSKFSEESFSDFVKKTHTKKIQSKRGVLSGKSRRIGSLEDTRPW
                     DDLGISRRKYFYMKKNGEGK"
                     /name="replication initiation protein"
     gene            3338..4210
                     /locus_tag="JJ463_27365"
     CDS             3338..4210
                     /locus_tag="JJ463_27365"
                     /inference="COORDINATES: protein motif:HMM:NF033610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="UMI17339.1"
                     /translation="MKYPSLYDVTNKRSASYQKRYFFFLRLEYCILILNAVNAFLTYEK
                     KHIVALVLFSMLMLMMVLKIKKTSEQEWYKYRAITESIKTVSWKFSMRAEPFNDEDEVK
                     NINSLSEYLEDIVGGSEFISKTIDPKSVDESTVTDEMKGLRTLSFEKRRDFYVDGRIND
                     QRDWYVSKAQANKDAGKKWNICMFVIYVLAFLCSLYNAYYSVPGSIIPISILTTIASSL
                     VGWIQVKRYSELSASYILTAHEIGVIKEQASYVSSESDFSSFIRDAETAFSREHTQWIA
                     RRVANRKPK"
                     /name="DUF4231 domain-containing protein"
     gene            complement(4228..4632)
                     /locus_tag="JJ463_27370"
     CDS             complement(4228..4632)
                     /locus_tag="JJ463_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017097459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="UMI17340.1"
                     /translation="MAREHKVFISHAWAHHDELESLRRLIDARPYFNAEYSEVSRNEPI
                     NSTNAPYIKSVLKRKILDSNILIGLAGIYASHSSWMEWELDTALANGIPVIGVVPRGAQ
                     RISTMVSTRAVEVVRWNTESIIAAIRLHAR"
                     /name="TIR domain-containing protein"
     Typing          2..1560
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.678"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgatcgtta aaattcactc acggggtgcg ggttcgggca gtgggcccgt ggattacctg
       61 ctgggtaaag accggcagcg cgaacaggcc cgcgtactgc gcggcaaccc tgaacatgtg
      121 cgcgagctaa tcgacggctg cgactttgcc cgcgcctaca catctggcgt gctgtcattt
      181 caggaacctg acattgcaga cgcggagaaa tcgcgcctga tggacgaatg ggagcacacg
      241 ctgctgaccg gcctcgattg tgaccagtac gcctgtctgt gggtagagca tcgcgacaag
      301 ggacggcttg agctgaattt tgtcatcccg aacatcgaat tgcagagcgg caaacgcctg
      361 caaccctact ttgaccgcgc tgaccgcccc cgtgtcaacg cctggcagac cctgaccaac
      421 gaccggctgg ggctgcgcga cccgaacgat ccgacttacc gccgcccgct gacgcaggcc
      481 agcgatcttc cccgcgacaa acagcaggcg gcagagaaaa ttaccgccgg cctgatgaac
      541 ctgatgcagc agggcatgat ccgcagccgg caggacgtgg tgacgcagct ggaaagctac
      601 ggcctgaccg tggcacggga aacgaaaagc agcatcagca ttgccgatcc ggacggcggc
      661 cgaaatatcc ggctgaaagg aatgatatat gagcgagatt ttaaatttgg cgaagggctt
      721 cgaggagaaa tcgaagcagc aggcgcagga taccgagctg agcgtgaagc gcgagttcgc
      781 gaggctggag acgtctatca gcgcggaact gcaatcaagc tcgcagagca tcagcaacgc
      841 tatccgcgag cagaacggca ggctgacggg catgatcaga acctccgtct cagcggcgat
      901 ggcgtggata ttcctctgct ttgccctgct gatcggtatc ctgggcggga taatctggtt
      961 tcagggcacg atcatcacct cgcgcctgag cgagatggac agctacagcc aacagctgga
     1021 gagcctgaaa gcgaaaagcg gcgcgggcgt gacgatttac agcgacgaca agaaccggaa
     1081 cacttacctg gtggtgctgc cgaagcaggc gcacggcgtg gaaacctaca catcgacgac
     1141 cgacaacacc gtggtgaaat acacggcgaa ataaccccgt cagaacgccc tgaatcgcga
     1201 ctgagcggtt ttaagaccga ggatgagcaa aaccatgacc gagatggaaa aacagcttct
     1261 gagcgcctta gaaagcttac agacaagtta cgagcaacag cagcaggcgt ggcaggacag
     1321 ctacagcagc ttgcagcaca tgttcgagac tacctcacag gcgctgacgc acagcgacag
     1381 ggtgtgtcag cacttgagca gtcaggtaga gagcttgcgc gcgcaggtcg agagcttgag
     1441 caggaacgtc agccgcttga tgcgctgatt gcacagcatg accgcgcccg tgaggagaag
     1501 ctgcgcgagc agcagccgga gccgcagaaa aaaccgaccc gctactatgg cccctcgatg
     1561 tgatcgcttt tccgaattca gattttggtg tactatgtac gccattaaaa acaggagaca
     1621 gccgtgagtc atgcgattga gtttatcgag accccgatgt ttacccgtca gataaaacag
     1681 atcgccacgg atgaagagct cagggagctg caaaagacgc tgattgagtc gccggacaaa
     1741 ggcgatctca tccagaaaac cggcgggctg cgaaaagtca gaatggcgac gggaaaccag
     1801 ggaaaaagcg gcagtgcaag ggtgatttac tttctggcca ccaccgaggt gatttatctg
     1861 gtgatggcct acccgaaaag caccaaagac agcctgacgg acgcggagaa agccgcgctg
     1921 aagatgctga cccaacaact gaaagacgag gtgtgacatg agtttttttg acgagctgaa
     1981 agcatcgctg gaagaagccg ttgaaatcaa aaacggcgtc agagagcctg cccgcgtcac
     2041 ccgttacgag ctggcagacg tgaaggccat cagggcacag ctgaacgttt cacagagtga
     2101 gatggcgaaa gtgctgggaa ccagtctcga caccatcaag agctgggaaa ccggacgccg
     2161 taacccgact ggactggcgg cgaaggtgct ggcaaccatc caggcgaatc caaaattctt
     2221 ccaggagctg gcggcgcact aagccgttgt gacagaagga ttcgacaggc gaggccgctc
     2281 acgtgtaatt taacggcaac agaaaaaaca aagccccgga aatcatgagc tatcttggcg
     2341 gatgagcatg actaccaggg ccaggtacga aaccttgaaa ggatattagc acatgaaccg
     2401 tgccagacaa cagcccgcca taagcgcagg gcacattgca taacgccgca ctcgaacttt
     2461 ttaacgaccg cttaccgcac aagccgtact tttcggacga tctgcacttt ggtgtacgca
     2521 ttgccggtaa ggaacgcgcc attctggcga aatacattca gttcaatcag cctcacgcta
     2581 tgttctggct gggattcgac gtcgatcgcg ccggtgccgc catcgactgg agtgaccgca
     2641 acgcgcccgc gcccacgctg accatcacta accctgaaaa tggacacgcg cacctgcttt
     2701 atgcgctgaa aacctcgata cgtaccgcgc cggacggaaa aatgaagccg ctgtgctacg
     2761 ccgccgccgt ggagaacgcg ctacgtaaaa aattagaggc tgacacgggg tattcgggac
     2821 taatctgcaa aaatccgaac cacggccact ggaaagttgc cgtatggcag ccggaactct
     2881 acacgctgga ctggttggct gattttcttg atttgaacgc cgcgaacgag aaagaaatcg
     2941 ttgccgacta cggattaggc cgtaactgca ccctattcga taaaacccgt aagtgggcct
     3001 atcgcgctat ccgtcagggc tggcctgagt atgagcagtg gctgcaagcc tgctacgaac
     3061 gcgccagcgc ctataatctg caattcgcag taccgctgga tgaaaatgag gttagtggaa
     3121 tagctaaaag tattgctaag tggacttcat caaaatttag tgaggaatca ttcagcgatt
     3181 ttgttaaaaa gacacataca aaaaaaattc aatcaaagag aggtgtttta tcaggaaaat
     3241 caagacgaat aggtagtcta gaggatactc gtccgtggga tgatttgggc ataagtagaa
     3301 ggaagtattt ttatatgaaa aagaatggtg aaggaaaatg aaatacccaa gtctttatga
     3361 tgtaacaaat aaaagatctg cttcatatca gaaaaggtat ttcttctttt taaggcttga
     3421 atattgcatt ttgatactta atgcggtcaa tgctttctta acttatgaaa aaaaacacat
     3481 agtcgcatta gtactgttct ccatgcttat gttaatgatg gttttaaaaa ttaaaaaaac
     3541 atcagaacaa gagtggtaca aatacagggc gattactgaa tcgataaaaa ctgtctcatg
     3601 gaagttttcc atgagagctg aaccatttaa tgatgaagat gaggttaaaa atataaactc
     3661 attatcagag tatctggagg atattgttgg tggtagtgag ttcatatcta aaactattga
     3721 tcctaagtca gtggatgaaa gcacggtgac tgatgagatg aaagggcttc gaaccctatc
     3781 atttgaaaag cgcagagact tttatgtaga tggaaggatt aatgatcaaa gagattggta
     3841 tgtaagtaag gctcaagcaa acaaagatgc agggaaaaaa tggaacattt gtatgttcgt
     3901 tatctatgta ttagcgttct tatgttcact ttataatgca tattattcag ttccaggtag
     3961 cataattcca ataagcattt tgacaacaat agcatcgtca ttagtaggtt ggattcaagt
     4021 taaaaggtat agcgaactgt ccgcttcgta tattcttacc gcgcatgaaa taggggtaat
     4081 aaaagaacag gcgtcatatg tttcaagtga gagtgatttc tcctcattta ttagagatgc
     4141 ggaaactgca ttttcgagag agcataccca atggattgct agacgagtgg ccaacagaaa
     4201 accgaagtaa ggggcaccgc ccctttttta tcgcgcatga aggcggattg cagcaataat
     4261 actttcagtg ttccatctaa caacttctac tgctcttgtt gaaaccatgg tagatatccg
     4321 ctgcgcacct cttggaacaa cacctataac aggtattccg ttagctagcg cagtatctaa
     4381 ctcccactcc atccaactac tatgagatgc atagatgcct gctaaaccaa tcaagatatt
     4441 agaatctagt atctttcttt tgagaacgga ttttatgtac ggtgcgttgg ttgaatttat
     4501 tggttcattc ctgctgactt cggaatactc agcattaaag tagggtctag cgtcaatcaa
     4561 tcttcttaat gattccagtt catcgtgatg cgcccatgca tgactaataa aaaccttatg
     4621 ttctcttgcc actgattgct ccttttactc aatcccctat gaacgcgcat tatacctctg
     4681 cgccgatccg ttttgcactc gaacctatat cagataacag cctctcttag aggggccgtt
     4741 ttttttgcag ccagcacaga atctgccata cttcacctta ctttaagcca gtacacacag
     4801 cgtcccctct aacgaggtgc caccgtgact ggttcagggg gctcgccgcc ccccgaaacc
     4861 cctggcatcc actgcgaaaa attctcacag tggtgcgaat tttttctcag cggagcctat
     4921 ggaaaagcga acaaaagaga tcaaaatccg gctcaccgaa actgagcatc agcgactgct
     4981 tgatcgctgt gaccggactc atcttgcaga gtggctgcgt cagcttggtc tgggagaaca
     5041 cacctcacgc aagcgccgtg tgcctgatgt ggctcccgaa cttctgcgac aggtcagcgg
     5101 gattggtaac aacctgaacc agatcgcccg tcgcctgaac cagactgatt ccctgacgcc
     5161 ttctgaacgt gcctcgctgc tcgtcgtact gaccagcctt gaccgtcagc ttggcgacct
     5221 gctggagcag aacc
//
