LOCUS       CP067839                8712 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3223 plasmid pC277_3, complete
            sequence.
ACCESSION   CP067839
VERSION     CP067839.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868986
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C277
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 186x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:48:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,476
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 52 of 120
            Pseudo Genes (incomplete)         :: 60 of 120
            Pseudo Genes (internal stop)      :: 29 of 120
            Pseudo Genes (multiple problems)  :: 19 of 120
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3223"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC277_3"
                     /geo_loc_name="USA: South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            1..468
                     /locus_tag="JJ463_27250"
     CDS             1..468
                     /locus_tag="JJ463_27250"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17328.1"
                     /translation="MLKHLNFPDALCAEVDAIRGDESFTSWLAGAARMRLDSGKHDQLD
                     RIEAMLKVLTGGHNAPVTPQEPPKKIWGGRPTKGPNNAPKKRQQAPRGGISPAQQVIID
                     LCNAAEGSEGLDQAIADELNRRGMLSPSGVEWTHRTIMNTRVRLRKRGLIS"
                     /name="hypothetical protein"
     gene            complement(461..1120)
                     /locus_tag="JJ463_27255"
     CDS             complement(461..1120)
                     /locus_tag="JJ463_27255"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17329.1"
                     /translation="MAKRTYSASIDERYWPAIDRLSAQLGCVRSSIMRMIARGELLVSV
                     NPDYVRTAEDEYSEKMAARLNDPVRKQGTIRKMIVDAGLNPPLGLVGDEEMAIEMASQA
                     AVIAERNKPEVKHEPENRPQTSAEKAEFIRLDAQFGAGGWLSGKPGGKYSADLDDAIRF
                     IVGRYVELSRRGRVGFMPVIAGELNDMAMPSFGGAKWRPVDVSWMVTKLRDAGYIN"
                     /name="hypothetical protein"
     gene            complement(1123..1635)
                     /locus_tag="JJ463_27260"
     CDS             complement(1123..1635)
                     /locus_tag="JJ463_27260"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17330.1"
                     /translation="MAKPESVKRMARLKRQKEREGAKDRRTKYNDDRPRELLAYVISWC
                     EMAGQVKTKRLGWRNDYRKHVGLSTSTITKWRRTHPAFARAELEAYHVYRTWLCDLAAE
                     GDERLTLGKPDRLYQNRQIIIDQQITIPVLKYGYSYGALRYTATLDPRDLVDNSQYVTI
                     KNRSGDR"
                     /name="hypothetical protein"
     gene            complement(3071..4687)
                     /locus_tag="JJ463_27265"
     CDS             complement(3071..4687)
                     /locus_tag="JJ463_27265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17331.1"
                     /translation="MSHQYEDLPQYGDDETDYAALEEYRSNVVQMPQPGAPAMTPEQIE
                     AALDAMPDGRSRVSFSQPTPAHKRPEHGANASELRREVESRKYDPDVAERNKRHLDAIM
                     GLDAPECATPADANIDYMRKNFVLVGGRLVLSLVDPTFAMKIDDAANHFTSITTDGMPG
                     LEHTKRGADGVEKRAFAVWLAKPKKENIVQQMTFSPGKPKMFYHEKYGRVFNRYTPLSW
                     DFSAVEDVTWFIDHLNNLWGRKARLFLEWLAYGIQKPEATMELAFLHINETRGSGRSKA
                     FEIIRKVYGRYAAPRLNLAEMLETKHNTEVDGNIFFAFDEILIEGVKRRKLAEAVKEFI
                     DNGTKQVNPKGLAKYEVVVNSRVLLASNYINGLPIEKGDRRIVVHIGNLPRLDIATAQR
                     LIAAAERKSFINSFAKFLMEYDVSNFNPGLVDVDDEDHARVVGATMSPEDKMIAEIINS
                     GVSILTSDMLTQLAKNYGLRGMPKDTLSAMKDAGKVTAQRLRVDGKQTRCWVFRDHERW
                     RSADGREVTAQVVSDRGLIDI"
                     /name="hypothetical protein"
     gene            complement(5325..6218)
                     /locus_tag="JJ463_27270"
     CDS             complement(5325..6218)
                     /locus_tag="JJ463_27270"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="UMI17323.1"
                     /translation="MSILKEIAAKAAALRGDLRDVKSSKGTRTMTPVYDDNHVSALAKS
                     IRQNAPEWLQLWFEISIETGGRTADIAGLRFSDLSASGELRYVVAKQSKAAMSRATRKG
                     VELVREQRKAAAKSAGQWELYAQTDAATADELAETLTDAEEAMVLEQVSRAQVKIDSKQ
                     LSPGLCKRIAAYRARTIGADDDSALMFPAAATGSNRAAGVWHKPVTRQTIWRLIKPFML
                     AIEKAGDGLMRLSAYSTRKIAAFRWMKAGDKQQPGSGITLVMEMLGHSSLAMSKKYLGL
                     DKLAARLQRGVALGTL"
                     /name="tyrosine-type recombinase/integrase"
     gene            complement(6215..6352)
                     /locus_tag="JJ463_27275"
     CDS             complement(6215..6352)
                     /locus_tag="JJ463_27275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17324.1"
                     /translation="MSATRILAVCLALVQGEKVRIPAMTAGELGELLNCVAMLKGEIMG
                     "
                     /name="hypothetical protein"
     gene            complement(6428..6616)
                     /locus_tag="JJ463_27280"
     CDS             complement(6428..6616)
                     /locus_tag="JJ463_27280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17325.1"
                     /translation="MKFREVRAGDLKVGDKVLSRGPQVVARIEGCFFDKREILMTFESG
                     LAGVWDKRDFVTLIVTE"
                     /name="hypothetical protein"
     gene            complement(6871..7863)
                     /locus_tag="JJ463_27285"
     CDS             complement(6871..7863)
                     /locus_tag="JJ463_27285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17326.1"
                     /translation="MPTQISDVIVPEEFTKYLVQNTMVASALFQSGVLANNDVVLAQLA
                     AGADMFNVPYWNDLSDDEADIVNDNPSDIAAAGTLNAVKQAVRKSFLHKGWSTMNLASE
                     LSGSDAMARVRERATAYWTRQGQKRLISMLMGIKAHNIANDGGDMIHDASTETIGATQV
                     IDAAGTLGDAMGAFRAIAMHSDLYKSLLKQDLIEYIRDSDGSLVMTTYRGLAVTVDDGL
                     VPDSNGVYTSVLFAAGAIGYGSAEPRIAAGTEIENLPGAGNGGGQQILHTRVNMAFAPL
                     GYGWTEASVAKESPSIAELAIAENWKRTAAERKQVPIAFLLTKGEAAAE"
                     /name="hypothetical protein"
     gene            complement(8034..8540)
                     /locus_tag="JJ463_27290"
     CDS             complement(8034..8540)
                     /locus_tag="JJ463_27290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMI17327.1"
                     /translation="MSDHDNHEDHEDHGEEQNPGLVKKNRQLLSEAKQSRARADELQAQ
                     LDAANSELTAIKLTNPVNDMLSDLVHPALSESARLATVGEFIDFALRDDGSIGVFKKDG
                     SEAGVEFSVTELREYLSKVSGGRLDSLLLAPESRGTGMHGNGGYRSNVPKQPAPAILSN
                     RLGIK"
                     /name="hypothetical protein"
ORIGIN
        1 atgttaaaac acctcaactt cccggatgct ctgtgcgcag aggtagacgc catcaggggc
       61 gatgaatcat ttacatcatg gcttgctggc gcggcacgca tgcggctgga ttctggaaag
      121 catgaccagc tggacaggat cgaggcgatg cttaaagttc tgaccggcgg tcacaatgcc
      181 cctgtaacgc ctcaggagcc gccgaaaaag atttggggag gtagacccac taaagggcca
      241 aataacgccc ctaagaagcg ccagcaggct ccacggggcg gaatatcacc ggcgcagcag
      301 gtaatcattg atttgtgcaa cgcagcggag ggttcagagg gactcgatca ggctatcgcg
      361 gacgaactca accggcgcgg catgctgtca ccgtctggcg tggaatggac gcaccgcacg
      421 atcatgaata cacgggttcg cctgcgaaag cgtggcctga ttagttgatg tatccggcgt
      481 cccggagttt tgtcaccatc caggagacat cgacgggccg ccacttcgcc ccaccaaagg
      541 agggcatcgc catgtcattc agctcgccag caataaccgg catgaatccg actcgcccac
      601 gtctggacag ttcgacgtat cgcccgacaa tgaaccggat ggcgtcatcg agatcggcgc
      661 tgtacttgcc gcctggtttc cctgatagcc agcctcccgc cccgaattga gcatccaggc
      721 gaataaactc cgccttctca gcactggttt gcggcctgtt ctccggttca tgcttcacct
      781 ctggtttgtt tcgctcagct atgacggcgg cctgagaggc catctcgatc gccatctctt
      841 cgtcaccgac aagccccaat ggcgggttta gtccagcgtc aacaatcatt tttctgatag
      901 tgccctgctt gcgcaccgga tcattaagcc ttgccgccat cttttcgctg tattcgtcct
      961 ccgccgtccg cacatagtca gggttcacac tcaccagtag ctcgccccgg gcaatcatcc
     1021 gcatgatgct tgagcgcaca cagcccagct gtgcggacag gcgatcaatc gccggccagt
     1081 acctttcgtc gatggatgcc gaataagtcc tctttgccaa agttaccgat cccccgatct
     1141 gtttttaatc gtcacatact gcgagttatc gaccagatca cgcgggtcta gcgtcgccgt
     1201 gtatctcagc gccccgtagc tgtagccgta cttgagcacc gggatggtga tctgctggtc
     1261 aatgattatc tgcctgtttt ggtagagcct gtccggcttg cccagcgtta accgctcgtc
     1321 gccctcggcc gccaggtcgc acagccacgt tcggtatacg tggtatgcct ccagctctgc
     1381 ccgggcaaag gccgggtgag tcctgcgcca cttggtgatt gttgacgtgg acagtccgac
     1441 gtgtttccgg tagtcattgc gccacccaag acgcttggtc ttgacctgcc cggccatctc
     1501 gcaccacgaa atgacgtagg cgagcaactc ccggggccgg tcgtcgttgt atttcgtgcg
     1561 cctgtccttc gcgccctctc gctccttctg gcgctttaat cgcgccatgc gctttacgct
     1621 ctcaggcttc gccatgtcct aagctcctca tctgcgccgg gaaccataac cagggagtgg
     1681 gcctcaatca aaagaatctc cctcacgatc ggcatctccg acaccgaccc gctgagtcgc
     1741 ccgggcgatc gtgcccgcga tgttaaaaac acgtctccag tgaagggggt ggcctgctct
     1801 ctgtgatgcc ctgctgtttc ccggcggtga tgcccctcag gctctctgta cgctcctgtg
     1861 cgccgccttc catgcctgcg gcataactac cgcaccgaag tggagatcgg ctcctgtggg
     1921 ctggctgcgg cggctgtgtg ttgatgcccg gtagggcatg gcgggctaat cggcgacttc
     1981 tggccgggta ggtggagtga cggggccaga ccgccaccct gcaaagcggg ttttcctgtg
     2041 acgctggccg gggatctgca ggtgtaataa cgcgcagtga tagtaacgca ctgaaatata
     2101 cgcctcgcgt gcgcgcgcga taggcggcgg tcggtcttat cctccccttc ctcaccactt
     2161 cgccagcatc gagacagggg tgagacagag cgagacagga gcgagacaga tgataaaaat
     2221 ctagtgtctc gggctgatag ccgcgccacg tctgggctga gcctgttttg cccccctatt
     2281 tttgagacag attaaaaaat gtactgtctt agctacaggc ctcgccacgc ctgggtttac
     2341 ctaccctgtg agacagtaag acactacttt tatgaatctt ttggaaaaaa taataaaaaa
     2401 ataaatatat aggaatttta aaaacaagtg tcttagtgtc tcgtttgctc ccagcccaga
     2461 gctggcgcgg cctacaggcg agacagaagg caagacagca tctgtctcag tgtctcgttt
     2521 tagcatgtat tttaaccagt ggtatgatga ctgtattatt ttcacccact gaatatagtg
     2581 gggggggact atattgggtt tccggcgatc cggtcatgac cgttggtgct ttcggtcgct
     2641 tagttggaaa atggacacaa aaaagccagc tctgcggctg gcctgtcgtg gtgggttgca
     2701 ggttttcctg cacccgtcgg cttggttacc ctcgcccagg ttgcggggga gcattcgatc
     2761 atggcctctc tcccgacgta caccggcacc tcccggccag ctacccatac ctcgaccagc
     2821 tcgccgtcga tcgcgactct ggtgacgccc ctcagtgtgg gcaatccgct tacccttact
     2881 ctgtcgcctt tccgtaggtt cttcgcccta acttgtttca tcgttttttg tgtgtcgtgc
     2941 tgtgtgtgag tcacgttttt aacatccata ttacggcctc gcaatagcta cacaggatct
     3001 atgtatttaa gagcacaaaa agcaaaagcc gccccgaaga gcggccctaa gttactgttt
     3061 cactggtctg ttagatgtcg attaatccgc ggtcgcttac cacttgcgct gtgacttcgc
     3121 ggccatctgc gcttctccat cgttcatggt cgcggaacac ccagcaccgg gtctgctttc
     3181 cgtcgactct caggcgctgc gccgtcacct tcccggcgtc cttcatggcg cttaacgtgt
     3241 ccttaggcat acctcgcaag ccgtagtttt tggcaagctg ggtgagcatg tcagaggtca
     3301 ggatagacac gcctgagttg atgatctccg ctatcatctt gtcttctggt gacattgtgg
     3361 cacccacaac gcgggcgtgg tcttcgtcgt cgacgtcaac gaggccggga ttgaagttgc
     3421 tcacgtcata ctccatcagg aactttgcaa atgagttgat gaagctctta cgctctgctg
     3481 cggcgatcag cctttgagcg gtggcaatgt ccaggcgtgg aaggttgcca atgtgaacca
     3541 ctatccgccg gtcgcccttc tcaataggta ggccgttgat gtagttggag gccaataaca
     3601 cccggctatt caccaccacc tcatatttcg ccagtccctt cgggttaacc tgcttcgtgc
     3661 cgttgtcgat gaactcttta acagcctccg ccagcttgcg gcgcttgacg ccctcgatca
     3721 gaatctcatc aaaggcgaag aaaatatttc cgtccacttc cgtgttgtgc ttcgtttcca
     3781 gcatttcagc cagattgagg cgcggcgctg cgtaccggcc gtaaacctta cgaatgatct
     3841 cgaatgcctt tgagcgtcca gatcctcgcg tctcgttgat gtgcagaaat gccagctcca
     3901 tcgtggcctc gggcttctgg atgccatacg ccagccactc gaggaacaat cgcgccttcc
     3961 ggccccaaag gttattcagg tgatcgataa accaggtcac gtcctcaact gcggagaagt
     4021 cccacgatag cggcgtgtag cggttgaata ctcgcccgta cttctcgtga tagaacatct
     4081 tcggcttgcc gggggaaaag gtcatttgct ggacaatatt ttccttcttg ggcttagcca
     4141 gccagacggc aaacgccctc ttttcgacgc catccgcccc gcgcttagtg tgctcaagcc
     4201 ccggcattcc gtcggtcgtg atgctggtaa agtggttggc cgcatcgtca atcttcattg
     4261 cgaacgtggg atcgacaagg ctcaagacca ggcgcccgcc cactaacaca aagtttttgc
     4321 gcatgtagtc gatattggca tctgctggcg tggcgcactc tggcgcatca aggcccataa
     4381 tggcgtctaa gtgacgcttg ttcctttctg caacgtcggg atcatacttt cgggattcca
     4441 cttccctgcg cagctctgag gcattcgcgc catgctctgg ccgcttgtgc gccggtgtgg
     4501 gctgtgaaaa tgaaacgcgc gaacgaccgt ccggcatggc atcaagtgcg gcctcgatct
     4561 gctccggcgt cattgctggc gcgcccggct gcggcatctg cactacgttg cttctgtact
     4621 cttcgagcgc ggcgtagtcg gtttcgtcgt cgccgtactg cggcaggtct tcgtattggt
     4681 gactcatgtc tggtgctccg tttttcgttg tagaaaaagg cgctggcccc acatgaaaac
     4741 ttgaaaggga tattcctatg tcgtaatatt cgctctgagt acctgcgcgg cctcacggct
     4801 cgctgtggtt ctcgcgattg ttgcggttaa tgcaatgaaa aggctatttt cgttgcgatt
     4861 cgcgcaccaa ttggcgataa ttacaagtgt tcaggttttc gttggggctg tatatcgtga
     4921 tcatcttgcc ggagtcacac gatatacggc ctttgtttta tctgcggttc ggtgaaccat
     4981 ccagatagcc tctcagcgtt cccgccttga tagctcgttg atattactcg atccacttct
     5041 tacgatccag atcttcccag gctcctcgcg gcctcagcgc ctctctgcgc gattttctca
     5101 cctcacccca tgcaatcccc ttaccagcgt tcacagcgcg cctaagtgac cgtttgaggc
     5161 gtctgtgaca taccccgggg aaaatatttg aggcgagtga aatcctcccc ttccatctga
     5221 ggaactccgg ttgataacat cacccccgct ggaatttcca cggctacctc gtctgtcaac
     5281 ccagcaaggc ccgggagcgc tgttttctac gttgcgcgac gtccttacag cgttcccagg
     5341 gctacccctc tttgcagtct cgccgccagc ttatcaagac ctaaatactt cttactcata
     5401 gccaggctgc tatggcctaa catctccatt accagcgtga tcccgctccc cggctgctgc
     5461 ttatcaccgg ctttcatcca ccggaacgcg gctattttgc gcgtgctgta ggcactgagg
     5521 cgcataagac catcgccagc cttctcgatc gccagcatga acggcttaat cagtcgccat
     5581 atggtctggc gtgtcactgg cttatgccac acaccggcgg cgcggttgct gccggtagcg
     5641 gcagcgggga acatcagcgc gctgtcatcg tctgcgccaa tggtgcgggc acgatacgcg
     5701 gcaatacgct tgcagagtcc cggggaaagc tgtttgctgt cgatcttcac ctgcgcccgg
     5761 ctcacctgtt ccagtaccat cgcctcctct gcatcggtca acgtctccgc cagttcgtca
     5821 gctgtggcgg cgtcggtctg cgcgtacagc tcccactggc ccgctgattt ggctgctgcc
     5881 ttgcgctgct ctcgtaccag ctccacgccc ttgcgtgtgg cgcggctcat ggctgctttt
     5941 gactgtttag ccaccacata gcgcagttca ccggatgcgc tcaggtcgga aaatcgaagg
     6001 cccgcaatgt ccgcggttcg cccgccagtc tcgatcgata tctcgaacca caattgcagc
     6061 cactccggcg cgttctggcg gatggacttt gccagcgcac taacgtggtt gtcgtcatac
     6121 accggcgtca tggtgcgcgt gcccttgctg ctcttaacgt ccctcaggtc gccgcgcagt
     6181 gccgccgcct ttgctgctat ctcttttaaa atgctcatcc catgatctcc cctttcagca
     6241 ttgccacgca gttcagcaat tcgcccagct cgcccgcggt cattgccggg atccttacct
     6301 tttcgccctg gactagtgcc aggcacaccg ctaatattct cgttgcagac attttgatta
     6361 tcctcccctt cggattagtc ttaatgcagc tccggggtta ggttgcagcc ttccccggag
     6421 cgtctcgtta ctcagtgacg atcagcgtca caaaatcccg cttatcccat acgccagcaa
     6481 gcccgctctc aaacgtcatc agtatctcgc gcttatcgaa aaaacacccc tcgatccttg
     6541 ccaccacctg cggcccacgg ctcaaaacct tatcgcccac tttcaaatcg cccgccctca
     6601 cttctctgaa tttcatagct acctcgtatc tattgtcgtg tgaggatatt acatcgggct
     6661 aatagtctat tcaatggcct taataggttt tgtcgatcga gttgataggc attgatcggc
     6721 taaatctcag ttgtggcgcg ggttggcggc ctctaagtta ccgaagaaat tttctgatat
     6781 ttaggcgctg aggctggatt ctggcgggct gggggtggat tgcaggcaca aaaaaccccg
     6841 cgcagtggcg gggtctgtcg tcgttgcggg ttattccgct gcggcttcgc ccttggtcag
     6901 caggaacgcg atcgggactt gcttacgctc tgcggcggta cgcttccagt tttcggcgat
     6961 cgccagctct gcgatggaag gggattcctt cgcaacgctc gcctcagtcc agccatagcc
     7021 cagcggggca aatgccatat taacgcgggt gtgcaggatc tgctggccgc caccgttacc
     7081 agcgcccggc aggttctcga tctcagtgcc tgcggcgatg cgcggctctg cactgccgta
     7141 gccaatagca ccggcagcaa acagcacgga ggtgtagacg ccgttgctgt ccggcaccag
     7201 tccgtcatcg acggtcacgg ccaggccgcg gtaagtggtc atcaccagcg agccgtcgga
     7261 gtcgcggatg tactcgatca ggtcttgctt gagcagggac ttgtacaggt cggaatgcat
     7321 cgcgatagcg cggaaagcac ccatagcgtc gcccagcgta ccggctgcgt cgataacctg
     7381 agtagcgccg atggtttcag tgctggcgtc gtgaatcatg tcgccgccgt cgttggcgat
     7441 gttgtgcgcc ttgataccca tcagcataga gatcaggcgt ttctggccct ggcgtgtcca
     7501 gtatgcggta gcacgttcgc gtactcgcgc catggcgtcg ctaccagaca gttcgctggc
     7561 gaggttcatg gtcgaccatc ccttatgcag gaaggactta cgcacggcct gttttaccgc
     7621 gttcagcgta ccggctgcgg cgatgtcgga cgggttgtcg ttgacaatgt ccgcctcgtc
     7681 gtctgacagg tcattccaat aagggacatt gaacatgtca gcaccggcgg caagctgtgc
     7741 cagtaccacg tcgttgtttg ccagaacgcc gctctggaat agggcgctgg ctaccatggt
     7801 gttctgcacc aggtatttag tgaattcttc ggggacgatt acgtccgaaa tctgggtcgg
     7861 catggcctat gctccaaagt agttattttg agggttattg cactgcttcg ggcggtctgc
     7921 actgcgtcgc gctcggtggc ctctggccgg gttttctgct gtcttggggc gctgccccgg
     7981 gttggctccc cgccgtcgtc tcagagtctg gcgggtcgcc ggtcgatcgg ctgttactta
     8041 atcccaagtc ggttacttag aatcgctggc gcgggttgct tcggcacgtt gctgcgatag
     8101 ccaccgttgc cgtgcatgcc agtaccgcga gactcaggag cgagcagtag ggagtcgaga
     8161 cgcccgcctg acactttgct caggtattcg cgcagctctg tgacgctgaa ttccacgcca
     8221 gcctcggaac cgtccttttt gaagacgccg atcgatccat catcgcgcag ggcaaagtcg
     8281 atgaactcgc ccacggtagc cagtctggca gactcactaa gtgccggatg caccaggtca
     8341 gacagcatgt cgttaaccgg attggtcagc ttaatggctg tcagctcgct attggccgca
     8401 tcaagttgag cctgtagctc atcagcacgc gcacggctct gctttgcttc actcagtagc
     8461 tggcggttct tcttcaccag tcccgggttc tgctcttcgc cgtggtcttc gtggtcttcg
     8521 tggttgtcgt ggtcgctcat ctcggatgcc ctcagggtcg cggtggatag tgggccaatt
     8581 atgtcaatcg ttcttctctc ccgtaagatt cagccggggt aatcacctat gtcatagcta
     8641 cccatttttg atgcccgata gctatgctgt tactatattt gaaccaagga atatagctgc
     8701 ggagtaccta ca
//
