LOCUS       CP067825               14098 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3288 plasmid pC282_3, complete
            sequence.
ACCESSION   CP067825
VERSION     CP067825.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN17131911
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 14098)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14098)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C282
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 337x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 16:16:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,322
            CDSs (total)                      :: 5,198
            Genes (coding)                    :: 5,118
            CDSs (with protein)               :: 5,118
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 28 of 80
            Pseudo Genes (incomplete)         :: 43 of 80
            Pseudo Genes (internal stop)      :: 20 of 80
            Pseudo Genes (multiple problems)  :: 8 of 80
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14098
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3288"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC282_3"
                     /geo_loc_name="USA: South"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            1..708
                     /locus_tag="JJ676_26570"
     CDS             1..708
                     /locus_tag="JJ676_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UMI01434.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            1191..2510
                     /locus_tag="JJ676_26575"
     CDS             1191..2510
                     /locus_tag="JJ676_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISKpn6 family transposase"
                     /protein_id="UMI01435.1"
                     /translation="MAASYLPYRPDQSYLLPPSLGEWLPEGHLAYFISETVDTLDLSAF
                     HARYAGGGPGNQPFHPAMMVKVLIYGYASGVFSSRKLARKLYEDVALRVLAAGNFPAHR
                     TLSDFRALHLTELENLFVQVVQLARECGLVKLGTIAVDGTKVKANASRHKAMSYKRMKP
                     AEDELHCEIKALLDRAKATDDQERNEPELDIPAEISRREKRLEAIQAAKARLEARQREA
                     DQARGRSEDDGRRPRHPDGSDKGGGSYKREFGVPDDRDQESFTDPDSRIMKHAGGGSEQ
                     SYNGYTAVDAEHQIIVAAELTNCAADSQALLGMLAAVQANTGEMPAQTLADAGFRSEAV
                     LAKVADHHGDVIVALGREGREDAKVNAKTHPHTAAIAAKLKTEQGDAAYRRRKSIVEAP
                     NGWIKAVMGLRQFSMRGLDKVQAEWKLVCMALNLRRMAYL"
                     /name="IS1182-like element ISKpn6 family transposase"
     gene            complement(2760..3641)
                     /locus_tag="JJ676_26580"
     CDS             complement(2760..3641)
                     /locus_tag="JJ676_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /protein_id="UMI01436.1"
                     /translation="MSLYRRLVLLSCLSWPLAGFSATALTNLVAEPFAKLEQDFGGSIG
                     VYAMDTGSGATVSYRAEERFPLCSSFKGFLAAAVLARSQQQAGLLDTPIRYGKNALVPW
                     SPISEKYLTTGMTVAELSAAAVQYSDNAAANLLLKELGGPAGLTAFMRSIGDTTFRLDR
                     WELELNSAIPGDARDTSSPRAVTESLQKLTLGSALAAPQRQQFVDWLKGNTTGNHRIRA
                     AVPADWAVGDKTGTCGVYGTANDYAVVWPTGRAPIVLAVYTRAPNKDDKYSEAVIAAAA
                     RLALEGLGVNGQ"
                     /name="carbapenem-hydrolyzing class A beta-lactamase KPC-3"
     gene            complement(4028..4807)
                     /gene="istB"
                     /locus_tag="JJ676_26585"
     CDS             complement(4028..4807)
                     /gene="istB"
                     /locus_tag="JJ676_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISKpn7 family helper ATPase
                     IstB"
                     /protein_id="UMI01437.1"
                     /translation="MNLQHERIDALCQQLKLERVATHYPVLAQQAVREDFSFTDFLEHL
                     LRHEAGCRQQRSRELLTRMACFPGIKTLEDYDFSFNPGVPKALVQELGSLAFVERAENV
                     VLIGPSGIGKTHLAIALGYKAAQAGVKTRFITAADLMLQLSTAQRQGRLKEYFNRAILG
                     PRLLVIDEIGYLPFGREEANLFFQVVAKRYEKGAMILTSNLPFSQWADAFAGDTTLTAA
                     MLDRLLHHAHILTLSGESYRLKDKRKAGVVRKNSKPE"
                     /name="istB"
     gene            complement(4804..5829)
                     /gene="istA"
                     /locus_tag="JJ676_26590"
     CDS             complement(4804..5829)
                     /gene="istA"
                     /locus_tag="JJ676_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="UMI01438.1"
                     /translation="MLTQEQTVEIRVLARQGHSIRHIARTLGVSRNTVRRYLRDPSVAR
                     YQPREPRPTKLGPFESYLRQRVEQAHPIWLPATVLNREIRAQGYGGGLSLLRAFLATLK
                     PARREAGPAVRFETEPGRQLQADFVVLRRARSPMSAFVATLGYSRMTFVTFVPDESFES
                     VRDSLLLAFDYLGGVPREVLFDNMKTVVLERDAYGDGKHRFHPGLLQLADDLGFRIRLC
                     RPYRAQTKGKVERFNRYFRESFYNPLLTRMKGTGLLLDCAAANRRVRDWLADEANVRVH
                     ATLNERPIDRWRQEREHLQPLPSRVRRDEAPLLDNSLRPVPLESLQHPLSVYDAIGEAC
                     R"
                     /name="istA"
     gene            complement(5936..8965)
                     /gene="tnpA"
                     /locus_tag="JJ676_26595"
     CDS             complement(5936..8965)
                     /gene="tnpA"
                     /locus_tag="JJ676_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family transposase"
                     /protein_id="UMI01439.1"
                     /translation="MSKKNKLLSVLSDAEQEALYGLPEFDDAQQLEYLAVTETELALAN
                     SRPSLHAKVYCLLQIGYFKAKHAFFSFDWDEVEDDCAFVLSRYFHGEVFEPKAITKHER
                     YTQREQIAKLFGYWPWSAAFLPQLEQQAARTVRRDVTPGFVAAELIVWLNEHKIIRPGY
                     TTLQELVSETLSAERRRLGNLLEQALDESAKAALSQLLVRDDTLSQLAALKQDAKNFGW
                     RQMVREREKRATLEPLHAIAKTLLPKLDVSQQNLLYYASLANFYTVHDLRNLKADQTQL
                     YLLCYAWVRYRQLSDNLVDAMAYHMKQLEDESSAGAKQSFADEQVRRQQETPRVGRLLS
                     LYVDDSVADPTPFGEVRQRAYKIMPRDTLQTTAQRMSVKPASKLALQWQAVDGLADRMR
                     RHLRPLYVALDLSGSNPDSPWLAALAWAKGVFAKQQRLSQRPLDEWPAATLPKRLRPYL
                     LTFDADGKPTGLHADRYEFWLYRQIRKRLQSGELYLDDSLQHRHFSDELVEMDRKIDVL
                     AKMEIPFLQQPVHAQLDALTAELRTQWLAFNRELKQGKLTHLEYNKDTQKLTWRKPKSE
                     NEKAREKAFYEKLPFCDVADVFRFVNDECKFLSAFTPMQPRYAKKVADADSLMAVIIAQ
                     AMNHGNHVMARTSDIPYHVLDSGYQQYLRQATLHAANDCISNAIATLPIFPYYSFDLET
                     LYGAVDGQKFSVERPTVKARYSRKYFGRGKGVVAYTLLCNHIPLNGYLIGAHDYEAHHV
                     FDIWYRNTSDIMPTAITGDMHSVNKANFAILHWFGRRFEPRFTDLNAQLKELYCADDPA
                     QYQACLIRPVGKIDCDLIHREKPNIDRIVATLGLKEMTQGTLIRKLCTYTTTNPTRRAI
                     FEFDKLIRSIYTLRYLRDPQLERNVHRSQNRLESYHQLRSAIAQVGGKKELTGRTDIEI
                     EISNQCARLIANTIIFYNSAILSRLVTKYEAAGNSKALALITKISPAAWRHILLNGHYT
                     FQSSGKTIDLDAIVAGLELE"
                     /name="tnpA"
     gene            9075..10790
                     /gene="tnpR"
                     /locus_tag="JJ676_26600"
     CDS             9075..10790
                     /gene="tnpR"
                     /locus_tag="JJ676_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn4401 family resolvase TnpR"
                     /protein_id="UMI01431.1"
                     /translation="MKTGQTTASSSTPVGFPDADSLAALRGWYAGLSSRAAVEHYLAAR
                     KAAGQSARGMLGHIRRQLIVFANSRHRPDLADVFQHAPEERQGRAGAVERAIRLLPTLP
                     APKPQISDSIDQWLSPRTVRALQAYGIHTLSALTVRIPRRRGWWKAIEGLGMTGARQVE
                     AFFAAHPQLTERARALIPIIQPSGIVPWEQLRLPNEVDGSQGNFRAPRNACTLNATNDY
                     EAVQAWLALHETVATQRAYRKEAARLILWAIVERGRALSSLTTDDAIAYRAFVRHPTPR
                     ERWVGPPRPRDSVEWRPFSGGLSARSAAYALTVLSALFRWLIEQRYVLANPFAGVKVRG
                     HALRPALDTARGFTEGEWLLLRAIADGLEWSYGWSEPAAQRLRFMLDFGYATGLRASEL
                     VGAVLGNVRLDEHGDHWLHLVGKGGKPGKVALPPLAYTALDQYLAQRQLPVSRERWNPK
                     TPLVGSLGEDGNAGITGPHLWCVLRRFFTQAAEAIEADHPVAAEKLRRATPHWMRHSHA
                     SHALARGAQLTAVRDNLRHASIATTSMYLHGDELERARQMRQAFGARHVKRGVD"
                     /name="tnpR"
     gene            complement(10975..13134)
                     /locus_tag="JJ676_26605"
     CDS             complement(10975..13134)
                     /locus_tag="JJ676_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UMI01432.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTEYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLATL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            13313..13567
                     /locus_tag="JJ676_26610"
     CDS             13313..13567
                     /locus_tag="JJ676_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UMI01433.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobilization protein"
     ARG             complement(2764..3641)
                     /name="blaKPC-3"
                     /gene="blaKPC-3"
                     /gene_name="carbapenem-hydrolyzing class A beta-lactamase
                     KPC-3"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(12777..13135)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          13136..13312
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..708
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10979..13221)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.599"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgacatcaa accctctgat agcctataag tccaatgcgc tggttgaggc cagttacaag
       61 ctaactttac aagaacagcg tttcttattg ctctgcataa gccgacttaa ctccggtact
      121 gacgtagctt cacctgaatt gcaaaagaca atgaccatta cagctgcgga atattttgat
      181 tcattccctg atatggggcg gaaaaatgcc gaggttcagc tacaggaagc gatcgatcgt
      241 ttgtgggaca ggtcgatcat tctcaaggac gacgagaagc gcgaggagtt ccgctggatt
      301 cagtatcggg cacagtacgc gaggggagag gggaaagcac aaatcacttt ttctgacgcg
      361 gtaatgcctt atctgacaca gttgaagggg caattcacca gagtcgtcat taaaaatatt
      421 tctaatttat caagaagtta cagcataaga atatacgaaa ttctccagca attccgcagt
      481 actggggaac gtattatcgc cctcgatgat tttaaatcat cactgatgct tgatggtaaa
      541 tataaagatt tcaaaacgtt aaacagggat ctaataaaac cctgtgttga cgaactgaac
      601 aaaaaaagcg acctggctgt aacggttgag acgatcaaaa aggggcgcac ggttgtagcc
      661 ctgcattttc gcttcaagga agacaaacag atcaagatga cgatctagaa tcgattttaa
      721 ggcacctaaa acgctccctg ctgcgttttt tttgtgttag tagtgcgaag gtactattga
      781 agtgaaaaat tgcgtcaaaa gcgctctaat gcagcgaaaa aagaaccccc atttttgggg
      841 gggtaagcgg gaaccccaga aaattccgcc attccgcatt gtggaatttt ttgggggggt
      901 ggtccgcggc atgacgaccc gccaaaggct accgcatcta tctgaggcgc tgtggcgcga
      961 cgctgaaacc ccggggaatt ccgctactcc agttccaggt ccgcaatagt tcgtccaggt
     1021 cgggtggcgt acgccggaaa cgccgatttt tccgcaaccg tttctgcgtt gtcggcaagg
     1081 tggtctcaga gagcgctcaa gaagcggttt ttctcgtatg tttagctacg gtggcctagg
     1141 agcctgcgcg gcagaagcca aatcggcgga agatcgagga cgatacgggg atggctgcca
     1201 gttaccttcc ttaccgaccc gaccaatcct atctgctgcc cccttctctg ggagagtggc
     1261 tacctgaagg gcatcttgcc tacttcatca gcgagactgt cgatacgctg gacttgagcg
     1321 cattccacgc ccggtatgcc ggcggtggtc cgggcaatca gccgtttcat ccggcgatga
     1381 tggtcaaagt gctgatctat ggttacgcga gcggcgtctt ctcttcgcgc aaactagcca
     1441 ggaagctgta cgaggatgtc gcgttgcgtg tgctggccgc tggaaacttc ccggcccacc
     1501 gcacgctgag tgacttccgt gccctacacc tgaccgagct tgagaatttg ttcgttcagg
     1561 tggtgcaact ggcgcgcgaa tgtgggctgg tgaagcttgg cacgatcgcg gtcgacggca
     1621 ccaaggtaaa agccaacgcc agccgccaca aggcgatgag ctataagcgc atgaagccgg
     1681 ccgaggacga attgcattgc gagatcaagg cgctgcttga tcgcgccaag gctaccgacg
     1741 accaggagcg taacgagccg gagctggaca ttcctgccga gatttctcgc cgcgagaagc
     1801 gcctggaggc gatccaggcg gcaaaggcgc gcctggaagc gcgccagcgt gaagcggacc
     1861 aggcccgggg gcgcagcgaa gacgatggcc gccggcctcg ccatccggat ggctcggaca
     1921 agggcggtgg ctcgtacaaa cgcgagtttg gtgtgccgga tgaccgtgat caggaaagct
     1981 tcaccgatcc ggacagccgg atcatgaaac acgccggtgg tggctccgag cagagctaca
     2041 acgggtacac agcggtcgat gccgagcacc agatcatcgt ggcggcggag ttgaccaact
     2101 gcgccgcgga cagtcaggcg ctgctgggca tgctggcagc agttcaggcc aacaccggag
     2161 aaatgccggc ccagacgctg gcggatgcgg gattccgtag tgaggctgtt ctggcaaagg
     2221 tcgccgatca ccacggcgat gtcatcgttg ccctcggccg cgagggacgt gaagatgcca
     2281 aggtcaatgc caagacccat ccgcatacgg cggcgattgc ggcgaaattg aaaacggagc
     2341 aaggcgatgc agcttaccgc cggcgcaagt cgatcgtgga ggctccgaat ggttggatca
     2401 aggcggtgat gggattgcgt cagttcagca tgaggggcct ggacaaggtg caagccgagt
     2461 ggaagctcgt ctgcatggcg ctaaatctga ggcgaatggc gtatctgtga gggcgaaggt
     2521 taaatggggc ggctcaaatg caccccagtc gtcataacac gccgcgcgcc gcaagattgg
     2581 tatccttggc gccgacgcct tgccaattgc agagagcgcc gccgccatcg tcagtgctct
     2641 acagaaaacc ggtcacacgg ctctgccgcg cagactccta gcctaaatgt gacagtggtt
     2701 ggtaatccat gccgcccgca agggcggcgg tggtgggcca atagatgatt ttcagagcct
     2761 tactgcccgt tgacgcccaa tccctcgagc gcgagtctag ccgcagcggc gatgacggcc
     2821 tcgctgtact tgtcatcctt gttaggcgcc cgggtgtaga cggccaacac aataggtgcg
     2881 cgcccagtgg gccagacgac ggcatagtca tttgccgtgc catacactcc gcaggttccg
     2941 gttttgtctc cgactgccca gtctgccggc accgccgcgc ggatgcggtg gttgccggtc
     3001 gtgtttccct ttagccaatc aacaaactgc tgccgctgcg gcgcagccag tgcagagccc
     3061 agtgtcagtt tttgtaagct ttccgtcacg gcgcgcggcg atgaggtatc gcgcgcatcg
     3121 cctgggatgg cggagttcag ctccagctcc cagcggtcca gacggaacgt ggtatcgccg
     3181 atagagcgca tgaaggccgt cagcccggcc gggccgccca actccttcag caacaaattg
     3241 gcggcggcgt tatcactgta ttgcacggcg gccgcggaca gctccgccac cgtcatgcct
     3301 gttgtcagat atttttccga gatgggtgac cacggaacca gcgcattttt gccgtaacgg
     3361 atgggtgtgt ccagcaagcc ggcctgctgc tggctgcgag ccagcacagc ggcagcaaga
     3421 aagcccttga atgagctgca cagtgggaag cgctcctcag cgcggtaact tacagttgcg
     3481 cctgagccgg tatccatcgc gtacacaccg atggagccgc caaagtcctg ttcgagttta
     3541 gcgaatggtt ccgcgacgag gttggtcagc gcggtggcag aaaagccagc cagcggccat
     3601 gagagacaag acagcagaac tagacggcga tacagtgaca tcaacgatat tccttgtttg
     3661 aaggtggagc taggtgtagc ggccggatgt aatcaggccg ctacagctgg gattatcacc
     3721 gagcggcaag ccgctccctc tccagaggct gtaacggctg taactgcccc ggatgggcac
     3781 acatcgggtt ggtaagtccc tggcaccaca acgtccagcg ctattgaaaa acgaatcagg
     3841 tcggcgttca tgggtcggcc acctcgcctg acctcggcat ccacgttacg gacggcctca
     3901 ggaagtcctg gccaagcccc gactattggg gcgcgaagat agcaccgagg gtacgccggt
     3961 gtcaacacca actgaaaact gacccacttt acccctgaat caccaaagta atactgaccc
     4021 acctggatca ttcgggtttg gaatttttcc tgaccactcc cgccttcctc ttgtccttca
     4081 agcggtagct ttcgccgctc agggtcagga tatgggcatg gtgcagcagc cgatccagca
     4141 tcgcggctgt cagggtggta tcgccggcaa acgcgtcggc ccactggctg aacggcaggt
     4201 tcgaggtcag gatcatcgcc cctttctcat agcgcttcgc caccacctga aagaacaggt
     4261 tcgcttcctc gcgtccgaaa ggcaggtagc cgatctcatc gatcaccagc agccgtggac
     4321 cgaggatggc gcggttgaag tattccttca gccgcccttg tcgctgcgcc gtgctcagtt
     4381 gcagcatcag gtcggctgcc gtgatgaagc gtgtcttgac cccggcttgc gcggccttgt
     4441 aacccaaggc gatggcgagg tgggtcttgc cgatgccaga gggtccgatc agcaccacgt
     4501 tctcggcccg ttcgacaaac gccagactgc ccagctcctg caccagtgcc ttgggcacgc
     4561 cggggttgaa gctgaagtca tagtcctcca gggtcttgat gcccgggaag caagccatcc
     4621 gcgtcagcag ttcgcggctg cgctgctggc gacaccccgc ttcatgccgc aacaggtgtt
     4681 ccaggaagtc ggtgaagctg aagtcctcgc gcaccgcctg ctgcgccagg acggggtaat
     4741 gcgttgccac ccgttcgagt ttgagttgct ggcacagggc gtcgatccgc tcatgctgga
     4801 ggttcatcgg caggcctccc caattgcgtc atagacagac agtgggtgct ggagagattc
     4861 cagtggtacc gggcggagac tgttatccag caggggggcc tcgtcgcgcc gcacacggga
     4921 tggcaagggt tgcaggtgtt cccgctcctg ccgccaacgg tcgatggggc gctcgttcag
     4981 ggtggcgtgg acccgcacat tcgcctcgtc tgccaaccag tcacggacgc gtcggttggc
     5041 agcagcacaa tcgagcagca gccccgtgcc cttcatccgg gtcagcaacg ggttgtagaa
     5101 gctctcgcgg aagtagcgat tgaagcgctc gaccttgcct ttggtctggg cccgatacgg
     5161 gcgacacagc cggatccgga accccaggtc atcggccagt tgaagcaagc cggggtgaaa
     5221 acggtgcttg ccgtcaccgt aggcatcgcg ttccagcacc acggtcttca tgttgtcgaa
     5281 caacacctcg cggggcacac cgcccagata gtcaaaagcc agcaacaggg agtcccgcac
     5341 ggactcgaag gattcgtccg gtacgaaggt gacaaacgtc atgcggctgt atcccagcgt
     5401 ggcgacgaag gccgacatgg gggatctggc ccggcgcaag accacgaaat cagcctgcaa
     5461 ctgccgtccg ggctcggtct cgaaacggac ggccggacct gcttcgcggc gggcgggctt
     5521 gagcgtggcg aggaaggcgc gcaataacga gagaccgccg ccataccctt gagcgcggat
     5581 ttcccgattc aggacggtgg caggcagcca gatggggtgg gcctgctcga cccgttgccg
     5641 cagataagat tcaaatggcc ccagtttggt ggggcgtggt tcacggggct ggtatcgcgc
     5701 taccgacgga tcgcggaggt agcggcgcac ggtattgcgc gagaccccca acgtgcgggc
     5761 gatgtgccgg atggagtggc cctgtcgggc aagaactctg atttccacgg tttgctcctg
     5821 ggtcaacatc gacggcctcg aaaaggccgc caaactagcc caggtggatc agttttactt
     5881 cgctggggtg ggtcagtttt acactgctgc taacacggaa ccgctgaaaa ttccgtcact
     5941 ccagttccag gcctgcgacg atcgcatcga gatcgatcgt cttgccgctg ctttgaaagg
     6001 tgtagtgccc gttgagcagg atgtgccgcc atgccgcagg cgaaattttt gtgatgagtg
     6061 ccagcgcctt gctgttgccg gccgcctcgt acttggtcac cagccgcgac aagattgccg
     6121 agttgtagaa aatgatcgtg ttggcgatca gccgtgcgca ctggttgctg atctcgattt
     6181 caatgtcggt gcgaccggtc agttccttct taccgccgac ctgggcaatg gccgagcgta
     6241 gctgatggta ggactcgagg cgattttgcg agcggtgcac gttgcgttcc agttgcggat
     6301 cgcgcaggta gcgaagcgtg tagatgctgc ggatgagctt gtcgaactcg aagatcgcgc
     6361 gccgcgtcgg gttcgtcgtg gtgtaggtgc acagcttgcg gatcagcgtg ccctgcgtca
     6421 tttccttcag cccgagtgtg gcgacgatcc ggtcgatgtt cggcttctcg cgatggatga
     6481 gatcgcagtc gattttcccg actggccgaa tcaggcacgc ctgatactgt gctggatcgt
     6541 cagcgcagta gagttctttc aattgcgcgt tgaggtcggt gaagcgcggc tcgaaccgcc
     6601 ggccaaacca atgcaggatg gcgaagttgg ccttgttgac gctgtgcatg tcgccggtga
     6661 tcgcggtcgg catgatgtcc gacgtgttgc gataccagat gtcgaacacg tggtgggcct
     6721 cgtagtcgtg cgcgccgatc aggtagccgt tgagcgggat gtggttgcac agcagcgtgt
     6781 aggcgaccac gcccttgccg cgcccgaagt acttgcgcga atagcgcgcc ttcacggtcg
     6841 gccgttcgac gctgaatttc tgcccgtcga cggcaccgta cagcgtttcg aggtcgaacg
     6901 agtagtatgg gaagatcggt agcgtggcga tggcgttgct gatgcagtcg ttggccgcgt
     6961 gcagcgttgc ctggcgcagg tactgctggt agccgctatc cagcacgtgg tacgggatgt
     7021 cgctggtgcg cgccatgacg tggttgccgt ggttcatcgc ttgcgcgatg atgaccgcca
     7081 tcaggctgtc agcgtcggcg accttcttcg cgtagcgcgg ctgcatgggc gtgaacgccg
     7141 acagaaactt gcactcgtcg ttgacgaagc gaaacacgtc ggccacgtcg cagaacggaa
     7201 gcttctcgta gaatgccttt tcgcgcgctt tctcattctc gctcttgggc ttgcgccagg
     7261 tcagtttctg cgtgtccttg ttgtattcga ggtgcgtcag cttgccctgt ttcagctcgc
     7321 ggttgaacgc cagccactgc gtgcgcagtt cggcagtcag cgcatcgagt tgggcatgga
     7381 cgggttgctg caggaagggg atctccattt ttgcgagcac atcgatcttc ctgtccatct
     7441 caaccagttc atctgagaag tgccggtgtt gcaggctgtc gtcgaggtag agttcacccg
     7501 attggagacg cttcctgatc tggcggtaca gccagaactc gtagcggtca gcgtgcaggc
     7561 cggttggttt accgtcggcg tcgaaggtca gcaggtacgg ccgcaagcgt ttcggaagtg
     7621 tcgccgcggg ccactcgtcg agtggccgtt gcgacagccg ctgctgcttg gcaaaaacac
     7681 ctttggccca ggccagcgcg gcaagccatg gactgtctgg attggagccg gagaggtcga
     7741 gcgcaacgta cagtggacga agatggcggc gcatgcggtc ggccagcccg tccaccgcct
     7801 gccactgcaa ggccagcttg ctcgccggct tcacgctcat acgctgcgcc gtggtttgca
     7861 gcgtatcgcg aggcatgatc ttgtaggcgc gctgccgcac ctcgccgaag ggcgtgggat
     7921 cggccacgct atcatcaacg tagagtgaca gcaggcggcc tacccgcggc gtttcctgct
     7981 gacggcgcac ctgctcgtcg gcaaaggatt gtttggcacc agcgctgctt tcgtcctcca
     8041 actgcttcat gtggtacgcc atcgcatcga ccaggttgtc ggagagctgc cggtagcgta
     8101 cccaggcata gcacagcagg tagagttggg tctgatcggc cttcagattg cgcaggtcgt
     8161 ggacggtgta gaagtttgcc aggctcgcgt agtacagcag gttctgctgc gagacgtcga
     8221 gtttaggcag cagtgtcttg gcgattgcgt gcagcggctc cagcgtggcg cgcttttcgc
     8281 gctcgcggac catctggcgc cagccgaaat tcttggcgtc ctgcttgagc gccgccagtt
     8341 gcgacagggt gtcatcacgg accagaagct ggctcagcgc ggccttggca gattcgtcca
     8401 acgcttgctc cagcagattg cccaggcgcc ggcgctcggc ggatagcgtt tcgctgacca
     8461 actcttgcaa ggtcgtatag ccgggccgga taatcttgtg ctcattgagc cagacgatca
     8521 gctcggcggc gacgaacccc ggcgttacgt cacgccgcac ggtccgtgcg gcctgctgct
     8581 cgagctgcgg caggaaggcg gccgaccacg gccagtagcc gaatagtttg gcgatctgct
     8641 cgcgctgggt gtagcgttca tgcttggtga tcgccttggg ttcgaacacc tcgccgtgga
     8701 aatagcggct cagcacgaaa gcgcaatcgt cctcgacttc gtcccagtcg aaactgaaga
     8761 aggcatgctt ggccttgaag tagccgatct gcaagaggca atagaccttg gcatgaagac
     8821 tgggccggct gttggcaagc gccaactcgg tttctgtcac agccagatat tccagctgct
     8881 gcgcatcatc gaactccggc aggccataca aggcctcctg ttcggcgtcg gataggacgc
     8941 tgagtagctt gttcttcttg ctcatggccc gttcttgcta tcttgaggtt tcgtagaaac
     9001 atggggcatt ttcgattcta ctgaaataat cgttattatc gcgttactcg cttttcgaat
     9061 cgcaacctgt ccatatgaaa acgggccaga ctacggcttc gagttctacc ccagtcggct
     9121 ttcccgatgc ggattcgctg gctgccttgc gcggctggta cgcgggactc agctctcgcg
     9181 cggccgttga acactacctg gcggcacgca aggccgccgg tcaatcagcg cgagggatgc
     9241 tggggcatat ccgccgccag ctcatcgttt tcgccaacag ccgccaccgg ccggatctgg
     9301 ccgacgtgtt ccagcacgcc cccgaagagc gacaggggcg cgcgggtgcc gtcgaacgcg
     9361 cgatccggct cttgcccacg ctgccggcgc cgaagcccca gatcagcgac agcatcgacc
     9421 agtggctgtc tccgaggacc gtcagggcac tgcaggcgta cggtatccac accctctcgg
     9481 cgctgaccgt gcgcattccc cgccggcgtg gctggtggaa agccatcgag ggactcggca
     9541 tgacaggcgc ccggcaggtc gaggctttct ttgccgcaca tccgcagctc accgagcgcg
     9601 cccgggccct gatcccgatt attcagccca gcggcatcgt gccgtgggag cagttgcgtc
     9661 tgccgaacga agtcgacggc tcgcagggca atttccgggc cccgcgcaac gcttgcacgc
     9721 tgaatgcgac caacgactac gaggccgtgc aggcctggct ggcgctgcac gagactgtcg
     9781 ccacacagcg cgcctatcgc aaggaggctg cgcggctaat cctgtgggcc atcgtcgaac
     9841 gcggacgggc gctatcatcc ctgaccacgg acgatgcaat cgcgtaccgg gcctttgtcc
     9901 ggcacccgac cccgcgcgag cgttgggtgg ggccacctcg accacgcgat tcggtggaat
     9961 ggcgcccctt ctcgggcggc ttgtccgcac gctcggcagc gtatgcgctg acggtgctct
    10021 cggcgctgtt ccggtggctc atcgagcagc gctatgtgct ggccaaccct ttcgccggcg
    10081 tcaaggtacg cggtcacgcc ttgcggcccg cactggatac ggcgcgaggc tttaccgagg
    10141 gagaatggct actgctgcgc gccattgcag acggcctgga atggtcctac ggctggtccg
    10201 agccggcagc acaacgcctg cgcttcatgc tcgacttcgg ctatgccacg ggcctgcgcg
    10261 ctagcgaact ggtcggcgcc gtcctgggta atgtccgcct cgacgagcac ggtgaccact
    10321 ggctgcattt ggttggcaaa ggtggcaaac caggtaaggt ggccttgccg ccgctcgcgt
    10381 atacggcgct ggaccaatac ttggcacagc gtcaactgcc agtcagccgg gaacggtgga
    10441 atccgaaaac gcccctggtg ggcagtcttg gggaagacgg taatgccggc atcaccggcc
    10501 ctcacctttg gtgcgtgttg cgaagattct ttacgcaagc cgctgaggcc attgaggccg
    10561 atcatccggt cgccgccgaa aagctgcgcc gtgccacgcc tcattggatg cgtcacagtc
    10621 atgccagcca tgcgttggcc cggggagctc agctcaccgc ggtgcgcgac aacctacgcc
    10681 atgcgtcgat cgccacgacg tcgatgtatt tgcatggcga tgagctcgag cgcgccaggc
    10741 agatgcggca ggcttttggc gctcgtcacg tcaagcgagg agtcgactga attttcatca
    10801 tacggaatga cggaattttc tgggattccc gcttagaacc cctttttcgg gggcggaaaa
    10861 acaaaactat gcttgcatta tgtgtgtgct tcttgcattc atccctgtcg atgaacatcg
    10921 aagaacatcg acgaacataa cacgatgtta aacaaaacaa aatattgttc acattcactg
    10981 tgaccaaccg cgatcatgtc tcctctcggt ggcgtgtgtt cgtgcatctt cctcgcgccg
    11041 ccattcgtcc caggtacgat taaaacccag cgagcggaac agatgatccg gctgtttttc
    11101 tggtgtgttg acggcagcaa aaagcggtgc ctcacgggtt ttcagatgaa actccataag
    11161 ttccggcgca ggtgatatgt ggctgtctgc atcgtgattt acggcaacac cataccctgc
    11221 ataccgtaac cccttatcgg tcagtgcact gatgaccagc tggctgttat cggctgaggg
    11281 aacggcaaac gcgggataca gcgtaccatt aagccggaca acaccgccca gagagtcatc
    11341 cacgcccatg accagcaaaa gctgaagctg gcgggtttcc cggtcgacag aactcattac
    11401 cggcatcagc gtcgctagca gacgtccctg cgtaaaatcc accttccttg ctgccagtga
    11461 tggcatggct ggctggcggt cttctccaag acagaaagac tccggccatg tcttcggctg
    11521 gcggagattg cgggtatatt gcggccatgt ctggcaaacc tgcgtcagaa gttgctcaaa
    11581 ggtcgcagag gaataaacac gttgcagtgg tggatgttga ccgatttcgc ttcggtcaca
    11641 gatataacca ctcctgtcac gcattaaatc aacacgctca aagattctgg tgcggtattt
    11701 ctccagctcg ttcccccaga gcgtgtattc tccgttccgg ttgtgaagca gaaggtaaca
    11761 ggtcatttcc ccgttaccag gcatgggggc ctcgccatac tccgtaacga ctgcgctcag
    11821 tacccctgaa tccataacgg cattgtcatt tttcctgagc tgttccgctc gtcgctccgc
    11881 gcgactacgt tctgtatcct cctgctcctt ccggcgcaat tcctcttcac gtttaagcaa
    11941 ctggactctg agagctgcaa ggccggatgc cccccactgc tgccactgtt tttcaaatgc
    12001 cgtctgactc gcgggaatgt cctgtttctc caccttccgg taatgctgtc gcaatttttc
    12061 catttgctgg ctcagcgact ggatttttcc ctgttcgcta cggtattccg gtgccgccca
    12121 ttgcatcagg ggaatacggg aaaaaccgga cgaaaacggc gatgattttt tcaggctggc
    12181 agcatggctc atcgcttcct ttcgttcggt gttcagctct tcccagcgtc tgcgcgtcgc
    12241 ctgcatatct gccatcatcg acatggcatt actggtgagt ttccatgccg cattactgtc
    12301 cttcggcaac gcttcgatga tgtcacgtag tgtgtacgaa gttgcgttgt caggaagcac
    12361 gatattcaga aggtcagcca cacggcgggc aggctctgtc agcccgctgt ttctgagttc
    12421 ggtacgggaa atctgtattt ccaggttaag tctgttaatc agacgattct gttcgccgcg
    12481 ttctgtttcg ataccgcgtg tttccatcgc acaggcagac tttccgaggt gaataccagg
    12541 cgttttctca agcccctgtc gctcgtatga acgatggtct atctcttcct gataaccggc
    12601 gttcgccagt gccctgttcg catggtcagc ccatgatgca cgccacgtct cggcatgtgt
    12661 ccggtcgttc cagtccctga cctttccacc gaatccagca ggtccgacag ctctcgtggt
    12721 cagcatgatg tgcgcatggg gatttgtttt atccatgtga tggaacgcca catcagcaat
    12781 catgccctga ctgacaaagt tttctctgac aaaggcgaga acggtttccc gtgcggcatc
    12841 ctgcggtagt tcgcggggga tggcgagttc aatttcccgc gcaagttgtg agttcttccg
    12901 gcgttcggcc ttctcaacgg cattccagag ttcggcacgg ttcgtacacc aggcaggggc
    12961 tgtcgcggga gtcagaatca cagcttcagc cacaccgctt ttgcgggtgt agtcgtgagt
    13021 cagtccggta cgctcatcaa gcatcagtga accggaacga taggcagcag aagcaacggc
    13081 gctgtagccg ctgtttctgg agatgatttt catgctgaga tgataaatcg ccatttctgc
    13141 cgtaatgcct ttttgtggtt ttctttcagc cccgcagggc gcacacaatc gaaacggagt
    13201 ggagattgta taagtgcgca cttcgtgatt ttccgtgcat tatgccagct cagtttttaa
    13261 actaccagtg acgcgctaaa atgcgatttt tcatcgcgca cggagttaca tcatgagcag
    13321 aacactcgaa cagaagattg ctgatgctga ggccagatta cagcgcctga aggcgaagag
    13381 caggagtctg gacacagcgc agaaggtcat tgtgggtgca gcattgctgg caaaggtcag
    13441 aaagccggag gaggtgcagt tgcgtgcctg gttactccag ttcctgaagg cagaggtgac
    13501 acgacaggct gatgtgacgc gcatactgcc gctgattaac gagctggaag cgctgccagg
    13561 acagtgattg tatcgtccgc tcgccgcagt cccagcgacc gagcgttagc gagcgagtgg
    13621 gcgaggaagc ggaagtgaat ccgtcgcctg attgagcaca tacgcacggt ttttcgggag
    13681 gtgatatgcg ctgaacccgc atgaatactg gatttccaca gggttggagg gggtgtattc
    13741 ggaacttgca attctgcttt tttgcaacgc attgttttta ttggtttttt gttttcagca
    13801 ctccaagtta tccacaggct cgtcgttaag tttttagatc ttgtttttaa gatcttgttt
    13861 ttaatacttg ttttagcgcc aaaattcctt atagaaaaca aaatgttaca acgctatagg
    13921 tgatatgatt taaggtaaag gtgatataat ttaaggtgct tgggtgaggt aatttaaggt
    13981 aaaggtgagg agatttaagg taatattcac aggaaaatgg ggggtgaaaa tataattgct
    14041 caccaatgct aaaggtgata ggtttttatt tcacctcatt tttggcctgg tggttaaa
//
