LOCUS       CP067717                3998 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-4850 plasmid pC346_7, complete
            sequence.
ACCESSION   CP067717
VERSION     CP067717.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15869025
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C346
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 190x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:24:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,479
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,231
            CDSs (with protein)               :: 5,231
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 66 of 121
            Pseudo Genes (internal stop)      :: 25 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4850"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC346_7"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            complement(139..1332)
                     /locus_tag="JJ502_27375"
     CDS             complement(139..1332)
                     /locus_tag="JJ502_27375"
                     /inference="COORDINATES: protein motif:HMM:NF015354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UMG43912.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="MobA/MobL family protein"
     gene            1576..1959
                     /locus_tag="JJ502_27380"
     CDS             1576..1959
                     /locus_tag="JJ502_27380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMG43913.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     gene            1963..2247
                     /locus_tag="JJ502_27385"
     CDS             1963..2247
                     /locus_tag="JJ502_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="UMG43914.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(2486..2749)
                     /locus_tag="JJ502_27390"
     CDS             complement(2486..2749)
                     /locus_tag="JJ502_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="UMG43910.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="plasmid replication protein"
     gene            complement(2746..3600)
                     /locus_tag="JJ502_27395"
     CDS             complement(2746..3600)
                     /locus_tag="JJ502_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UMG43911.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     Typing          complement(323..1332)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 agctgtggca atacataaag gggaaataga accggcgagg atcacaactc taaaaatgta
       61 gtatcttata gtatagtatt aaataatatt aagttgtatt aagtgatact actatatagg
      121 aatatatacc actatatact acattccaaa tttcttcttc ctcttaggtt gctgatcttc
      181 gaattccggc attggttccg gctcaggctc ttcaattttt ggttgtggtt ggttcatagg
      241 agacattcca gagcctgaac ccatttttcc gcctccatta tttccaccgg ttccggttcc
      301 tgtgcctgtt ttaggttttg tttctaactc tgtctttaat atatgttcgc caaattcatt
      361 tattacataa ctactaacga aattttgagg atcttctttt tcttcttttt tatttttatt
      421 tacattaggt tttgatttaa atatatgctg ggtctttttg tataatgcct ttaaattatc
      481 aatgattgac cttgcaagag gctttttctc tattttcata gtttcctctg gctgaggtgg
      541 tttggtttgc tgtaagtatg ttgcattagc tatttttaat aataatgctt tttctttttg
      601 ctccatagct cttaattttt gaccttttcg ccattggtgc ctgtgtatcc tggtcattgg
      661 cgttctgtcc agttcaatag tttttgaaat ccacttggct ttctctttcg gagttttagc
      721 aaaaacagcc tttctttctg cctcttcttt ttggtctttt aatgaacggg agtcaatacg
      781 agatttatga ccatattttt ttaatcggtc atttgctatt atttcccagt taactcgcca
      841 ggcttcaatg ttttctctat tattccattc tctttgtttg ttcccaaatc catttgggct
      901 aacagttctt aaagttagca ttacatgagc atgagggtta tgactatcaa gatcatgaaa
      961 tgctatgtct gcaatcattc cataatccac gaaattttct tggcagtaat cgaggacgat
     1021 ctgcttcttc tggccgtcgt ccagctcctt ggggatggct atgtccagat aacggcataa
     1081 ttcggcatcc tttcgcttct cgccagcctc tacggcattc cagagggtgc ctgagtcctt
     1141 caccagttcg gcaggtgtgc cggcagggga gaggatagca gagccgaaaa atccctcttt
     1201 ttttgaataa tcccaggttg ccccggttcg attatctttt gtcttttctc ggttttgata
     1261 tgcagataaa taaaccgaag atttaccttc tgatcttcgt aattttttaa agctgatatg
     1321 aaatgtcgcc atagtctacc ccttactttt tttgtctttt gaattttggt ttttagcccc
     1381 gcagggccca tacgtcgcaa gcgacgtgta agtgggcatt actaatatta ttttttcggt
     1441 tgcacctcaa aataataagt aatgaacccc aggcttcgcc caatattaaa tattatttgt
     1501 tttattctaa aatatatttg actaaagtaa ataatggagg taataaataa caaaataaat
     1561 aattaggagg ttattatgaa taaacttata acagaggcag aaggtgcaaa aatcgaaagt
     1621 gtttttaaat acatattaga ggcatctgaa agcatattaa tagagcatgg gcatgttatt
     1681 acggggcaaa taataaaaaa caaaaagttc tttgctgata acgggcataa agattttgaa
     1741 aggttagagt attaccttag ctcggcaatt agttctcttg tcgaggataa ggtaacagaa
     1801 ggcattaaag acattaacac ggatgagaac cttgttttta aaaaaataaa tgaattgagt
     1861 gacaaggaaa aattaatttt agcagagtgc attattaaac attttgaata ttcaaatgtt
     1921 agaggaatgc aaaaggcatt taatattttt ggggggtaat ggatgagtaa aacgacagca
     1981 cagcagatcg cagatttgca aaataaacta caagcattaa gaacaaaaaa agcaagacag
     2041 gacagaaggg aagaaacaag acaaaaaata atcctgggtg cagaagtggc taaggtttta
     2101 ggtaaaaaac cggaggaaat taacaaggct ttaattcttg ggttgttaag taaaataaat
     2161 gaacttgacg aagaagaaaa acaagaattc acacgattag gattaaatat tttagaagag
     2221 tggaagaatg aaaatcgtaa accgtgaaca ataaaaaacc agccgatctt ggctggtttt
     2281 ttactgtgaa gggttgccag gcttgcctgg aggtcttaaa aaggctcaga gaggctctca
     2341 gggcttatgg gtgggtttgt tgattgttgg gtacctgagt gcctctcagt tcgagataca
     2401 gggcccggat acggggaaag gggaaaaaat ccccccaggg gtggagggtt tggttaggtt
     2461 agggggaaaa actgcttaga agtcgttaca ggctgttttg aggcatatct aaggcaacat
     2521 aacggcgata cagggcaacg gctccgtgat acgaacagcc cattttgtcg gctacttgtt
     2581 gccatttcag cccctggctt cgtagttcgt aagcctgacg acgacgatcc attgctgttt
     2641 tttcgtaatc gtcacgagat tgagaaaaat attttcttac agttctttca ctacgacctg
     2701 tcatttccat tatttctttt gcttttattt ttttaccatt aatgatcata aaaccccctt
     2761 gctcattaat atgtctaaca tttcattttt aacattggtt tttcgaggtt ttttggctct
     2821 gtttgattgg atttgtgaaa atgtatggct atcaaaattt ttccaagtcc aacgggctac
     2881 acttctaact gttgctttta tctctttgta ttgtaacggg ttttgtggat tgcaaaaagt
     2941 gtttaagccc tgtgccttta atagtagttc gttttcgaat ttctcgaaag tggatatttt
     3001 tttgaaattt aaaaccgttc tgtaagcata ttgccgtaga tcttcaaaca aattaacatt
     3061 gcgacctaaa ccataagcct cgcttttttt cttttgtatt tgaggctttt ctaagtccac
     3121 aaaatcagct aaataatcca ggtcataaag gtcagcacct gaccagaacg gcgaccagtg
     3181 atcattgagt gggttttttg taatcaatcc agcaaacccc agatctgctt ttagtttttt
     3241 cgccatagcc atttcaacgg ctgcggcata cttcaacggc tttaaccttg cttgttgtgt
     3301 cttacaaacc ccggctttca gcatatagcc gtagtgacaa cgacctgttt ttggtgtttt
     3361 tgtaataaag tgaggtatgg gtaagcccac ctgctcaaat gtatagaatg aatttttctt
     3421 atctagatca aagagtaagc aagtttgaat ggctggctga tttgcctgta aatacagcat
     3481 ttcaagggct tttgccttgt tccttatgct taatcccata tcgaggttat ttgtacaata
     3541 aggcttgtta ggcatttgat ttttaaaaaa atcgatgtta tgttcttctt gtattatcat
     3601 tttttcagtc ctttttatct ggccctactc tctgttttct gtcgccaaaa caaaatcgca
     3661 gagggtgggg tttttatttt tatagatttt tcaatcctgt accagttcta accgatctca
     3721 aaaatttgtc aagtgtgcgt ctaaccatat cagataacag cccaacgaag ttggggcagt
     3781 tctttacata cggcagcaaa gcattactat cagatctatg gtgcgaagca ttaccgaaga
     3841 tagaaaaaca ataaaaaaag aaaaaagagt attgttcgaa gaaaaattgg cgattcgatt
     3901 ttttttggtg aatatattaa aaaaaagaaa gtgcagaaaa aacggaaaag aaataagcat
     3961 agtaaatttt ttaaaatgct tatgaagtca gttaatga
//
