LOCUS       CP067716                4863 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-4850 plasmid pC346_6, complete
            sequence.
ACCESSION   CP067716
VERSION     CP067716.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15869025
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C346
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 190x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:24:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,479
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,231
            CDSs (with protein)               :: 5,231
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 66 of 121
            Pseudo Genes (internal stop)      :: 25 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4850"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC346_6"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            13..381
                     /locus_tag="JJ502_27350"
     CDS             13..381
                     /locus_tag="JJ502_27350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMG43906.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(618..731)
                     /locus_tag="JJ502_27355"
     CDS             complement(618..731)
                     /locus_tag="JJ502_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UMG43907.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1147..1338
                     /locus_tag="JJ502_27360"
     CDS             1147..1338
                     /locus_tag="JJ502_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMG43908.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1338..1760
                     /locus_tag="JJ502_27365"
     CDS             1338..1760
                     /locus_tag="JJ502_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UMG43909.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            3505..4803
                     /locus_tag="JJ502_27370"
     CDS             3505..4803
                     /locus_tag="JJ502_27370"
                     /inference="COORDINATES: protein motif:HMM:NF024700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="UMG43905.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     Typing          complement(619..1026)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 tcaaagagca ttatgaaaac tacagataga tttatttctg ttagttttag atattttcat
       61 aacgtcgatg atattggtca gtctattgat tcttggaggg ttgacggcag tttgccagat
      121 ttaattgaaa agcttgttta tcttacagct aatgggataa caaaagtcag ctctgatggt
      181 atttttacta actacactcg atttccagat tcagcagtta atgattttaa atgccctgat
      241 gaaatatcag ttgatgagaa ctggggtgtt ataaaaaata taggaggcca gaaaagaagg
      301 gttgctggtt ttttaaagga taatgtgttt tatatagttt tttttgataa ggatcatcgg
      361 ttttggaaga gcaggaaata ggcttagtta cgattgtact gtatgttgag gcaagatctt
      421 cttgagatca ctgtgttctc gtcgtaatct ctcgctctgt aaacgaaaaa accgccttgg
      481 cgggcggttt tttcgaaggt tccaagagtt ggcgctctta gaaccaaggt aactggcttg
      541 gaggagcgca gtaaccaaaa cttgttcttt cagtttagcc ttaaccggcg cataacttca
      601 agactaactc ctctaaatca gttaccagtg gctgctgcca gtggcgcttt tgcatgtctt
      661 tccgggttgg actcaagacg atagttaccg gataaggcgc agcagtcgga ctgaacgggg
      721 ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag tgtctggcgt
      781 ggaatgagat aaacgcggcc ataacagcgg aatggcaccg gtaaaccgaa aggcaggaac
      841 aggagagcgc acgagggagc cgcctatcag aaatgatagg tatctttata gtcctgtcgg
      901 gtttcgccac cactgatttg agcgtccgtt tctgtgatgc ttgtcagggg ggcggagcct
      961 atggaaaaac ggccgctgcg cggcctcctc ttttcctcct accttgatcg ctcggttttc
     1021 tcgagctttt ataagaatgg tcttgccgct cgccgcagcc gaacgaccgg agcgtagcga
     1081 ctgagtgagc gaggaagcgg aaaagagact ggttttacac tgagcactga cgctctgagg
     1141 cttcttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat ccagaatcag
     1201 tctctgctcc tgctggagaa gcttaacgag cttgctctcg atgtcgaggc cgacctttgt
     1261 gagaaactcc acgatgatgc ggagcatctt ttccgtacac tgtcctcgag actggatgaa
     1321 ttacaggatg gtaactgatg gccagagtgt ctattagtga agcggcacgg cttgttaagg
     1381 ttagtcgccc cacaatttac aagatgatta acagtgggaa gttaagttac acctctgttg
     1441 taaagcacgg taaggctatt aaagttattg atacttcaga gcttattaga gtgtttggtt
     1501 ctcttgatgg tgtcatcgat actgtaaaat atgatgtaaa aagtgacgca gaatctacag
     1561 gtgttaacag cgttggttta catgatttac aacatagaat cgctttactt gaagctgaaa
     1621 acgatggtct taaaggggct gttaaggctc gagatgagca tattgattcc cttcgtcagg
     1681 ctatgcagct tctggagcac aagcacgaac cttcttcacc accccattcc ccgtggtgga
     1741 aattctggaa aaaatcctga ccccgtcatg cttccgtttt cctctccggc catcccgggg
     1801 agttcctgaa ccttcccaaa aatcgtcaga tctctgaacg ctcctcacca ctaaaaatac
     1861 ccacacccca cgcaaaatta acattttgca gatttgtatt taatatcagt gggttacttt
     1921 cctgaaattt cgttttttaa attttattta tatagatccg actctatgac gagtcgtcat
     1981 agagtccccg gcgagtcgtc atagagtcgg taacgactca ggactataaa aacaataagt
     2041 taataacgac ccgactctag acacgactcc ccgtttggtt gttttttaga ctcggcgact
     2101 ctgggtacga ctctagtgaa gattcgccgg tgaatcgtgt ggtgagtcgt ccggcgactt
     2161 gccataacgt gattgccggc cgtcgacgaa cccgatttct gaacgtgctg aaaaccggtt
     2221 ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt
     2281 ttcggatggg aagaacgccg gaaacaggac ttaccctggc tgaatggcgt gaattttcgc
     2341 gctatgcact tgcgtgcata ctcatgcatg ccgtaaaaac agagcctgcg cgtttctggc
     2401 gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg ccctgaggcc
     2461 gttttagcgg tgcgtacaat tagggggtta tgttaaatcc acaccagcta cagacgtaaa
     2521 aaaagccccg cagggcttta tatctatata tctgttctca tccaatccca tgtgtcttgg
     2581 gtgagccatc ctgcgtattc tttggttaca ccaatcacaa gcaaggtgtc attattgtcc
     2641 agatgcctac gcaaacgatc cgacagttgt tgcgctgttt ctccggttga tattagccat
     2701 gtggaatcta aagggtggca ccaccccggc gattttttta actcatcata tagtgcattg
     2761 tagttttgac ctgcttgatt caggtcataa gagacacagt aaactgacat agaaccatcc
     2821 tctttaatca aatgtggggc agcccgttag ggctgcagtc acaacttact tcttcttttc
     2881 acgattaatt ggaattttga acatcccacc gtttggacgg gtgatccgtt tgccctttac
     2941 tgtgatataa gggcgaaaaa tgtatgcctg agactcgtct gtctgcatgg tgcatttcct
     3001 atttttcgcc acatgagtgt tgatttttgc tatcaaaatt gtactgtagc aaccaacaaa
     3061 tcttgtggct ggctttatca agctcttgag gtggtactca agtgccgcat ttgtgtatgg
     3121 tttgttacgg gttagttctg gtggtacagg ctaacccact tacttcaaaa aacgtcgttt
     3181 ttctcctttc atgcgtcatt tcctagatgt ttcttcactg tatcagctcc gctgagcata
     3241 aatgctacaa agagaaacca tccccatcct gaaaccgagt gtaaagctag cactgctgag
     3301 ctgacaactg cggctaaagg aaccatttgg tatagaaaaa atctcataga ccccctgcat
     3361 ttagcttttt ttaacaagtg ttccataacg tgctatctat taaacgcacc tgttgtttta
     3421 gaacgcttgt atgctatcct taagtcctga ttttgtcaat atttactcat tttgttgttt
     3481 aaggatggcg taaggggtgt tcttatgatg gttaatttta gtctttctaa cgcattttcc
     3541 tttatggatg ctcaaaatat gagcatgttg gcggtgtcat ccgcgaaaga cgacataaac
     3601 ttggctaacg tggtttcagt gagtgatgtt cggatactga aatctgctct gatatttggg
     3661 gctaacgcta gcgggaagtc taatctcatg aaaacaattt ctttaatgaa agatattgtt
     3721 ttgcattccg tgcaagctat tgaaagtagc attacaaaga acgtaatacc gtttttgctt
     3781 gatgaggatg gaccaaataa accatctgaa atggaaatga cttttatttc agatggggtt
     3841 aaatatagat atggattgtc tatttttcag gggaaaataa aagaagaatg gttgtactac
     3901 acaccaaaat caagagaaac cattctcttt gaaagagatg gtctttctat tgaggtaaat
     3961 aaggcggctt ttagtgagtc aaaccttttt atcaaagaag gggttattca aaaaacaagg
     4021 gaggatgttc catttgtttc ggttgttgct ggttttaatg gtgagcattc aaaacgagta
     4081 gttaattggt ttaacaatat cgtattcctt tctggttcta atgaagggat gtttacagca
     4141 attaccatga agttaataaa tgagtctgag gattttaagg cttggctaaa aaaaatactt
     4201 ccagcattcc agattaatga tatttacctc gatgaggtcg cacctgattt tccatttgga
     4261 aatataaaaa cagatgatga taatttaaaa aatttattta gttcaataca aaattttgca
     4321 aaaaaccaga aggttttttc tcttagagta gtgaaaaaaa cagctagcgg tgagatttca
     4381 gtacctattg attttgagtc tgagggtact aaaaagatag tttatcttct tggtccaatc
     4441 tacaatgcca tgataaacgg taacttattg ttagttgatg agtttgattc taagtttcac
     4501 acccttttgt ctaagttttt gtttagaata ttccatgcgc aaagcgaggg gcttagccaa
     4561 ataatagctg ctgttcagga tgttaatcta atgaacactg attgttttag acgtgaccag
     4621 atttggtttg ttgataaaga tataaaatca ggaagtagtc atctctattc tttggttgag
     4681 tataaagaga agcagcgaac tttaaaatca tcgtatgggc atgattattt atctggtgca
     4741 tttgatgcta tcccattgtt tgataattta gatgaatttg aaaaacttat gcctgaggga
     4801 tgatttatgt caaagagagg ccgcggtctt cgtagctctt cgaggaaaga ggaaacaaaa
     4861 tcc
//
