LOCUS       CP067715                4991 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-4850 plasmid pC346_5, complete
            sequence.
ACCESSION   CP067715
VERSION     CP067715.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15869025
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C346
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 190x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:24:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,479
            CDSs (total)                      :: 5,352
            Genes (coding)                    :: 5,231
            CDSs (with protein)               :: 5,231
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 52 of 121
            Pseudo Genes (incomplete)         :: 66 of 121
            Pseudo Genes (internal stop)      :: 25 of 121
            Pseudo Genes (multiple problems)  :: 20 of 121
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4850"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC346_5"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            join(4858..4991,1..298)
                     /locus_tag="JJ502_27320"
     CDS             join(4858..4991,1..298)
                     /locus_tag="JJ502_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMG43901.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="hypothetical protein"
     gene            complement(392..796)
                     /locus_tag="JJ502_27325"
     CDS             complement(392..796)
                     /locus_tag="JJ502_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="UMG43902.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(1591..1992)
                     /locus_tag="JJ502_27330"
     CDS             complement(1591..1992)
                     /locus_tag="JJ502_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UMG43903.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1992..2183)
                     /locus_tag="JJ502_27335"
     CDS             complement(1992..2183)
                     /locus_tag="JJ502_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMG43904.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3095..4153
                     /locus_tag="JJ502_27340"
     CDS             3095..4153
                     /locus_tag="JJ502_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="UMG43899.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(4183..4440)
                     /locus_tag="JJ502_27345"
     CDS             complement(4183..4440)
                     /locus_tag="JJ502_27345"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMG43900.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     Typing          2305..2707
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="7.72e-165"
ORIGIN
        1 atggactgag ctttcggcaa aggtttggcg aaacacagga gaaaaatggt caagacaatc
       61 agaagagctg cctcttcacc gggtaatcga ccttgcaact ctgatctgta tcaccatgga
      121 ctacagtaaa aatgggcgac tttcatccag tgatgatttt cccgtgactc gctcaactga
      181 taaccctgag ctagcacatc acactgagtt tatgaaaaaa gagttaaaac aaaataaaag
      241 caatcttgat gtaagcctca aacgcctctc gactgaattg aagagacttg gctattaaaa
      301 ctagctttag aaatgtctac ctacttcaca gacgaattta ccataacatc cgtttttcga
      361 accataatgg caggctatag cctgccgtaa ttcaccggaa aaaatcatcc acagagtcgc
      421 tccactcatg cccggaaaga gaccacacct tgtttatagg gggaggataa ccactcctgc
      481 agtacccgtt ttttcccacg aaatcgaatg ccccgtgcgg gtatccattg atcaggagta
      541 cgcacttctg actgtcttct gaccagccaa ttttaacatc agaaggaatg tgaccgtctg
      601 aaacgtcttc ggcattgtag atgtgtactg cgtccataat cgggtttccc tcgacggact
      661 catcgagcgc atagaaatac ccggtccggc catcgtcttc gaacacggcg gccagcatgc
      721 cctcaggagc gaggctcttt ataacctgtt cctccccgac aacgagctgg gcctgtatcg
      781 tcactaaaat ggtcatcagg tctgttctcc tttccttggt tgcttcggca gccagctttc
      841 agacaagcgg ataatttacc ataacatccg ttatcagcat cgtcagaaac gcgctgaggg
      901 cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg
      961 cgcaggctct gtttttatgg catgcatgag tatgcacgca agtgcatagc gcgaatattc
     1021 acgccattca gacagggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac
     1081 ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aaccggtttt
     1141 cagcacgttc aggaatcggg ttcgtcgacg gccggcaatc acgttatggc aagtcgccgg
     1201 acgactcacc acacgattca ccggcgactc tgcactagag tcgtacctag agtcgccgcg
     1261 actaaaaaac aaccaaacgg ggagtcgggt ctagagtcgg gtcgttatta actcattgtt
     1321 tttatagccc tgagtcgtta ccgactctat gacgactcgc cggggactct atgacgagtc
     1381 gtcatagagt cggatctata taaataaaat ttaaaaaacg aaatctcagg aaagtaaact
     1441 actgatatta aatacaaatc tgcaaaattt taattttgcg tggggtgtgg gtatttttag
     1501 tggtgaggag cgttcagaga tctgactatt tttgggaagg ttcaggaact ccccgggatg
     1561 gccggagagg aaaactgaag catgacaggg tcaggatttt ttccagaatt tccaccatgg
     1621 cgccggggat tcagttaatt ttgcctggtg attttcaaga aggagcatgg cctgtttcag
     1681 gctgtcaatg tgcgaatctt tggacaaaag taaagtgttc aggtgttcaa tctcctgttt
     1741 cagatgacga atcacctgtt cacactggtc aacagaggct gtatcgcgct gttcagagac
     1801 ctgtccagaa tggtgttcac ccttcagttg ttcaaaggga ccaaagacac gtaaaagctc
     1861 tgaagtatct accatttttg cattgcgctc accgctacag gtggacaggt cacctgcttt
     1921 aattaacctg tgcagtgttg ttctactctt acccgtcagt cgggctgcct cactaataga
     1981 cactctggcc atcagttacc atcctgtaat gcatccagtc tcgaggacag tgtacggaaa
     2041 agatgctccg catcatcgtg gagtttctca caaaggtcgg cctcgacatc aagatcaagc
     2101 tcgttaagct tctccagcag gagcagagat tgatgctgga tgaatttagc catgttcagc
     2161 gctgttttct gttgtttcgt cataagaggc ctcagagcgt cagtgctcag tgtagggatg
     2221 ttctcttttc cgcttcctcg ctcactcagt cgctacgctc ctgccgttcg gctgcggcga
     2281 gcggcaggag catgcgtatg aaagctcgag aaaaccgagc gatcacgtaa tttagaaaag
     2341 ggaaggccgc acagcggccg tttttccata ggctccgccc ccctgacaag catcacagaa
     2401 acggacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatat caggcgtttc
     2461 cccctgatgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     2521 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     2581 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgactgctg cgcctgttcc
     2641 ggtaactttc gacttgagtc caacccggaa ggcacgacaa aacgccactg gcagcagcca
     2701 ctggtaaggg gtgtacaaga attactggtg cagagttctt gaagttacag cctaactaca
     2761 gctaaactga aaggacagat ttggtggcag cggtcttgta caccctgtta ccacggttca
     2821 gaagttcccc aacttactga tccttcgaaa aaccacctcc ccaggtggtt ttttcgttta
     2881 cagagcaaaa gattacatcc cgcaggttag gatctcaaga agatcctttt aatcggaatt
     2941 cgacaactcc agataagtca tggtgagcca gccaacccct tcgactgaat atcgttccaa
     3001 aaaaaggcag tcagctgaaa gaaactaaca gtagaacaat aatctctagt accctgaatc
     3061 taagctaaca acaaaactag attaggagtt atagatggcg atctatgccg tatggaataa
     3121 caagggtggt gtagggaaaa gttaccttac ttttcagtta gcctctgaat atgccagaca
     3181 gaaccctact aaaaaggtcc tggtggttga cctgtgccca caagcgaact cgtcctctat
     3241 gttacttggt ggaatgataa atggcgaaaa cacactaaac cagattcatt cagcaataca
     3301 gagaaagaca atatctggat atattgagga taggatcaga agtccatata ttcctaccaa
     3361 cactggttct agctatgtaa taaatgtatc tacacacaat agtaaagttc caaataatct
     3421 atatcttgtc actggcgatg aacaattaga actccaagca tcaagagttg ttggggcatc
     3481 atttccaggt ccccatgatg catggcgtac tgttcacctc tggattaatg atttaatcag
     3541 tgacatttgc aactcatgga ataatgaaga tagctgcgta ttcattgact gcaaccctag
     3601 cttctctatt tatacagagt tagctttaac cgcagcagag cgccttttaa ttcctttctc
     3661 tgccgatggt tcatcaaaga gagcagttaa ggctgtactt tctttagttt atggtgagcg
     3721 tcgccatcag aatgaaccac aatcagattt ctatcttaat agcaatcgtt attctatgtc
     3781 gctaccacaa atatatatgt atgttggaaa tagattaacg caaatgaaca attcatctgc
     3841 cagtgcattt aagacggtag taaccgaaat tggtgatgaa atttggaaag tatggcaaac
     3901 aaaaccaagt ctgttttgta ttcacccaaa tgggagctca acgccaaata ataaaagatc
     3961 tttcaggaat atgtttcagt atgaagtaaa tgatgcaaac acagcatctg tcgtatctgg
     4021 agccttaggt attccaatcg cgacattaac tgcaggaaat aaaactgttt caggaaaaat
     4081 aataaaggtt aatcagacgc agctagataa acaagtcccc aacattgtag ctctcgctgg
     4141 tatgatcgaa taaaatagca aggcccttaa aagggccttt ttttatttgt ttttctttac
     4201 tgcttgcagc gccctgccag cactagaggc caactctttg attacattat ctttactttc
     4261 aacaacctca gtaagcaact cgttttcgtt ttccagttta ttcaaagcgt tctgagtatc
     4321 ttcaatagca tcatcatacc cttcggcata gccatccatc caaccaagag tatacgtttg
     4381 gtccaaaaga tcagcttgct tatcctgcag atctttattc tgattattgt tagagcccat
     4441 gttttttcct catttgttgg cgcatctgag ttttcaaacc aggaggaatt ccatttctaa
     4501 gtttctcact tagtctgact ggtggtgtgt ttcctgagac tggattacag tcagcatcca
     4561 ccccgacatc tttcttttta tagtcgccaa tattcctaac gttatctgta gtcatagaaa
     4621 accacgccca ttgctgtgaa taaccgaact ataaaccaga aaaaaaacgt acggcttatc
     4681 ataacacttg acacaaaaaa acaccgctac tctccaagca aaaaacataa aattggaaaa
     4741 acaaaatcac aagcacatga taacaataga tattaatatt tacttacatt tagtgagttt
     4801 tataattaga aaaacaatgt aatttaataa tcacaaacat tacctaagga cgaactgatg
     4861 aaaatacccg cgcatttgaa gcataaacca attattcaag tcgaaaatta cgaccgcatt
     4921 gacggcccac atgccgatga cactgatgct atggggctat ctgtaggtat tgctcaatgg
     4981 aacactcctg g
//
