LOCUS       CP067702                9506 bp    DNA     circular BCT 02-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3520 plasmid pC4688_4, complete
            sequence.
ACCESSION   CP067702
VERSION     CP067702.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868198
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C4688
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 618x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 16:08:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,807
            CDSs (total)                      :: 5,679
            Genes (coding)                    :: 5,589
            CDSs (with protein)               :: 5,589
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 32 of 90
            Pseudo Genes (incomplete)         :: 55 of 90
            Pseudo Genes (internal stop)      :: 13 of 90
            Pseudo Genes (multiple problems)  :: 10 of 90
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3520"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC4688_4"
                     /geo_loc_name="USA: Northeast"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            1..180
                     /locus_tag="JJW80_28985"
     CDS             1..180
                     /locus_tag="JJW80_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMG33334.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(208..738)
                     /locus_tag="JJW80_28990"
     CDS             complement(208..738)
                     /locus_tag="JJW80_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMG33335.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(745..1476)
                     /locus_tag="JJW80_28995"
     CDS             complement(745..1476)
                     /locus_tag="JJW80_28995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="UMG33336.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(1476..3404)
                     /locus_tag="JJW80_29000"
     CDS             complement(1476..3404)
                     /locus_tag="JJW80_29000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="UMG33337.1"
                     /translation="MPELARQIYPWVSWLYQEYYGLAGAFLVIGLFFGLAELWAGRMRF
                     VGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNYAYWLTGAAGLAL
                     LGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLAVGIVLARLLAFL
                     LLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNALADFSSEQARQE
                     QAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRCVRDFGDAVVYFD
                     PKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLIAGLNLGETGDVA
                     DHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLITRLENVADLSVLQ
                     TDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFREWPHVSVML
                     DEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFVKTTVLDNTPIRW
                     FYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQKEARPLFDVNTLQ
                     HLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEFRPPADGGGPKAD
                     DDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5133..5282)
                     /locus_tag="JJW80_29005"
     CDS             complement(5133..5282)
                     /locus_tag="JJW80_29005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UMG33327.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5367..5624)
                     /locus_tag="JJW80_29010"
     CDS             complement(5367..5624)
                     /locus_tag="JJW80_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="UMG33328.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(5634..7319)
                     /locus_tag="JJW80_29015"
     CDS             complement(5634..7319)
                     /locus_tag="JJW80_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="UMG33329.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            7722..8141
                     /locus_tag="JJW80_29020"
     CDS             7722..8141
                     /locus_tag="JJW80_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UMG33330.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8447..8710
                     /locus_tag="JJW80_29025"
     CDS             8447..8710
                     /locus_tag="JJW80_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="UMG33331.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            8700..8999
                     /locus_tag="JJW80_29030"
     CDS             8700..8999
                     /locus_tag="JJW80_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UMG33332.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9061..9438)
                     /locus_tag="JJW80_29035"
     CDS             complement(9061..9438)
                     /locus_tag="JJW80_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="UMG33333.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     BGC             634..9506
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4452..5030
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(749..1476)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atgacaaaac aggaaaaaac agccctcaat atggctaaat ttattaagga gcagtcgctg
       61 ctattgctcg ataaactgaa tgagctggat atggataccc cggccgacat gtgcgagcgg
      121 ctacacgagg atgcggaaac actctggcag gtgatgcggg ataagctcgg ggaggaataa
      181 cagcaccggc atggccggtg ctggcattta ttttttgccg aacggccacc accggcgggc
      241 aggtttgtcc tccggcagtt caggtaccgg cacgttaaaa tagcggctgt tatattcccg
      301 catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cactcaccac
      361 gtcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc
      421 gttctcgatg gcgaaccgct ccaccttgcg atagaggtaa tcacacagct tcattcctgt
      481 gggtttgttc cgttcccatt gcggattttt accagtgaaa aagtacgtgg ctttttcgtc
      541 gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg
      601 gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca tatcttccag
      661 atgctcgctg gtcggcatga tgcagcgctc gccgttccgg tcctccgcgt cttcaataaa
      721 taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc acgcaaacaa
      781 ccgttcacga tccagctctg ccgttttgcg ggcctcgccg gtctgtgggt tcacgatttc
      841 atccaccgac aggattgccc gctgcaccct gacctgcgtc gcccggtccg ggcagatgta
      901 ccagatttca tcgatgccgt tagctttgcg gttgaacaga tacccggaca ccacttcctg
      961 ataccggcga cggctcttga cggtgcgctc cacctcaaac gccaccgttt taccgtccag
     1021 ctccgccagg gcgtccggca ctttcatgcc tttaagactc agacggtgca gaatgcgcca
     1081 gccggtggcc cctctggctt ccatatcaag gcgcagggat tgcacggcca gccggtgcgg
     1141 cagatgtgct gccgacaccc ttgacggctg aaacggaata acatccgcca gcggttcgtc
     1201 ctcgtcgaac gagaacgcca cgccatgcgg cgtcagcccc cacacgctga cctgacggcc
     1261 gtccaccgga tatttggtcc ggaccagcag ccctttcgcc accaggctgt cgaggaagcg
     1321 gtagaccggc tgcaccgccg caatccccag ccgccgcgca accagctccg ccgtggaaca
     1381 cagctcttcc cgcaaccagc gcagggtcag ccgggttttc tccgcgttcc gcgcctgtcg
     1441 tttcgcgtgg ctgacgttgt acagctccag cgccatcagt aaatccccgc aaaatcatca
     1501 tccgctttcg gcccgccgcc gtcggccggg ggccggaact ccgccagggg atcggttttc
     1561 gccagcaccg ggaaacgctg caacgggatt tcccggcgct caacccgcag cggactgctg
     1621 aacgccatcc gggccacacc cagcccggtc atgaccgcga acccgtccgg caggtgttgc
     1681 agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc cccgctgata
     1741 tgctccacgt tccccgcctc ccggctggca cggcggcgct ccacgtccac cctgatttcc
     1801 ccggtctgcc ccgccgccca ctgcgcggac tccaggctgg ccgcccggta aaaccagcgg
     1861 atcggcgtgt tatccagtac cgtggttttc acaaattcag cgggcagatc ctgcccgcac
     1921 tgcccgaaat cgcccagcga ctggtgcgcg agcaacagat tgcagtcccg gctgcgcagc
     1981 gtccccagtg cgttcagtac atacttcgac agcagatatt taatttcatc gagcatcacg
     2041 ctgacgtgtg gccactcccg gaactcgtcc cgcgcaatga tgatttgcgc gcagcgggca
     2101 aacagcattt tttgcacacg gatcaccgcc tcgtcgtcca tcgagccgat aacgtacagg
     2161 cagccgccgc cgttgataat ccccgccaca tcgatgccac tgtccgtctg caggacgctc
     2221 aggtcagcga cgttttccag cctagtgatc aaccccttca cgtccttttt cagggcctcc
     2281 ggcagcgcgt acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg aaactgctcc
     2341 gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc
     2401 tcgcccaggt taagcccggc aatcagcagg tttttcagcg cgtactggtc gagatcgcgg
     2461 aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccggacag
     2521 tgcgcccgga agacgtgcgg cgcccatgcg tcccctttgg ggtcgaagta caccaccgcg
     2581 tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcacgct cttgccgctc
     2641 ccgggcaggc cgaggatctg aatgttggtc ttgcgccagg tatcacgcgg aaccagcacc
     2701 ggcttgccct gctcatcctt acccagaaac accgcctgct cctgccgggc ctgctccgag
     2761 gaaaaatcgg ccagcgcatt aaccggtacc ggctccccga aatgcaggtg gcgcacgtcg
     2821 gtcatctctc ctgtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc
     2881 gcctcgtcag cagccgcaaa atgaccgtca agcagcagga acgccagcag ccgcgccaac
     2941 acaatcccga cagccagccc gcccgcgcac caggcaatcc cgaacaggtt agccggctgc
     3001 agcaggtaat aatctgcgtt tgcgtagagg tccggacgca ggacggtttc tccccagctc
     3061 tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggccgc gccggtcagc
     3121 cagtacgcgt agttccagac atgtcgacgt gccctgacca tccgcccggg cgaatccagc
     3181 gaggccagca ccgaaaatgg cgctggccgt ttattatcgc gcttatagcg atcggcatac
     3241 tcccgtcgct ccttatgact cagccccaca aaacgcatcc gtccggccca aagttcagcc
     3301 agaccgaaaa acaggccgat aactaggaaa gccccggcca gaccgtaata ctcctggtac
     3361 agccagctga cccacggata aatctgccgg gccagctccg gaatgtgcca cttccaggca
     3421 agcgaatccg tattaaacgg ggtttgacgc tcaacggaac ggtttcacac gttaaggact
     3481 ttttgtccga taaatcagaa tattgtggac agtgaaaaat gtgcctaaag cggtgtgttt
     3541 ttaatcggca aatggcactt aatggacacc attgtccatt aatcgatttt tcagacgact
     3601 tctctcgcct aacgtgagtt ttcgttcgac tgagcatcag ccccctaaac atgttctctc
     3661 tccttcagac gatggtttgc cggcgtgatg tgcagccccc gctgcacaag ccgcggacat
     3721 gccccaaggc atggcggcgg cggtcacgtc cgtggtgatt gcgttcagct ttcaccacgg
     3781 aggccagcct ctttccgccc catgcggaaa aggcgcagcc ccggaggaac aacgagcacg
     3841 ccccagcgtg tgagaggaaa aggcggcacc ccagtgcggt cttttccggt gttccggccc
     3901 ccgtcagagt tcgccatagc gagcggctga ccacccgcaa aatcaggagc agctttgctg
     3961 cttcgccagg tgcgtccagc accgtggatt tttgtgggtg gtcaggcgtg agcctgacgg
     4021 gggccgggtg aatttctggc gtggccggcg tccagccgga cggagccaga aagaggggga
     4081 gagaccgtgc gtcggtggcg tccagccagc gtaagcaggg gatatccccc tgaggtcagc
     4141 cacgccctcg cggggatgac ctgtatttct ccgtaaggag aaaaaagcaa cagagatagg
     4201 ttaacgtaaa atgttcagcg aaaaaccgat atgattcacc tatcctggta atttattatc
     4261 agggaatata aagacttaat caggggacaa tatgccacat cagcgcgtcc ggtgtccgtc
     4321 ttgtaggtgc cggggaaatt gcgcctcctc gctcactcgg tcgctacgct ccggccgtga
     4381 gactgcggcg ggcgctgcgg acacatgcaa agttacccac agattccgtg aataagcagg
     4441 ggactaacat gtgaggcaaa acggcaaggc cacgcaggtg gcgtttttcc ataggctccg
     4501 ccctcctgac tgagttcaca taaacagacg ctttttcggt gcatctgtgg gagccggtgg
     4561 ctcaaccatg aatcagtcag gagtggcgaa acccgacagg acttaaagat catcaccgtt
     4621 tccgatgaat cgctccctct tgcgctctcc tgttccaacc ctgccgtttc ccggatacct
     4681 gttccgcctt tctcccttgc gggaagtgtg gcgctttctc atagctcaca ctctggtatc
     4741 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaaccc cccgttcagc
     4801 ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacgcaa
     4861 aacgccactg gcagcagcca ttggtaactg ggagttcgca gaggatttgt ttagctaaac
     4921 acgcggttgc tcttgaagtg tgcgccaaag tccggctaca ctggaaggac agatttggtt
     4981 gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt ccccaactga cttaaccttc
     5041 gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa
     5101 aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc cgtcagttca
     5161 gaggaggacg atggtgccac cgttccaccc tgaacatccc ggatatagtt tgcctgacat
     5221 gcgaccagaa gaaagcctga aaaaatcaat ataaaaagaa aaacaacctt tacctttttc
     5281 atataccttc cctgtattta aaaaccccgg tattcatccc tgaacaccgg aagtctgtat
     5341 ttcaaatcat ccttgataac ttttgcttac cagttaccat cccggtaaac aaaggataca
     5401 aagtaatcaa atttactaat atcgattaca tttttaaagt gtggctgtaa tatcgaaacc
     5461 catgattttt caacatcaaa ccaaccatta ttaatattat cctttaaagg catccccaga
     5521 ctttcaatga cagaaccatc atcaccgaag tcttttgagt attcaccacc tttaaactct
     5581 tcggttttct tatcaaacca atgaatatgt aatttaagcc ccatatttac ctcttaaaga
     5641 tattttttaa tgtttcgttt tggatccggc cctttaacct gcttacccgt ttttggatcg
     5701 aatgccccga ggtgttcgcc atcactggcg cggtaccctt caagctcacc gtgctgggaa
     5761 tcccattcat aaattttacg ctttttatca ccataccatc gggcccgctt accaccacca
     5821 ccttgtttag gggttttagg tttaccctct ttcaactctc ccaacccctt aatatcttca
     5881 gtcttgggat caggaaaata atcatggccg taatctttag ttcccttacg aggttttttc
     5941 ttttcctcat ttaatttatt ttctgcatcc ttcgcttttt tctccttttg tttccggctc
     6001 tccatcgccc cggaaagcgc agccttatcc ctggataagg attcattcat ttcgtttaaa
     6061 gatgtctggg cagcttttaa tttctcattc tgattctgaa tatccttatc aagatagcga
     6121 acctgatcct gccacccgga gccatattca tgaggcgggt tataagtcac aaaatcatct
     6181 ttatttgcct ctgcctcttt taccttttta tcggcttcct gaacagcagc ccttgcaccg
     6241 gggatcgcct tctctgtcga tgcaatgcga ctgtttaagg tcgcaatatt tttatcttca
     6301 gcatccagct cagctttcgc tttatcatac tctcttttca gcccctcttc cgggtgagca
     6361 gcgtcccatg cctgctggcg acgcttttct tcctccagac gctgttttac ctgtgccgga
     6421 gtaagaacat ccgtcaccga cacatagacc ggtttctgtc cgctctcttt cgggaaacga
     6481 ataacggcct cacgggagtt accaccggcc gtaaaaccag ccggacgtga atcacctttt
     6541 tcagcaataa tgccttttgg cggggctttc gcggccggaa cacctttagg tacgctcacc
     6601 tgcagagacg ggagacccgg aatcgacaca ctgaataccc ccggacgttt tgtcggtttc
     6661 gcatccacca caggaacact cattggccgg ccggtgacaa ccgcaatatg ctgccgctcg
     6721 tccttcacaa tatccgccac acgtttagtg acgctgaccg tcgcctggtc cagcggcagg
     6781 gagctgaccg gcgtctccgt taccgtgtct gccggcaatg acgtcacgat ttttgacatc
     6841 attttcggat catcttttgc tatctcagaa ggcagcactc cgtaaatcgc aatcccccac
     6901 agaccaaact taaacggacc tttcagggca gccagcacat cagcaacagc ggccgacaac
     6961 gcatcgccgg aaacggataa agccagtcct tcagcccccg gagtggccag ggcaggcaga
     7021 ccgaaggcca tcgcggtggc cgaagactgt ccgccaccag agctcccgct atgattgccg
     7081 ctgttaccgc cattaccacc gggtttactc ccgccgttac caaactcccc cgggttatag
     7141 ttatgaatat ccccgttagg tgtatgcgtc gtaccccagc ccggcccgct gtttggatcc
     7201 gtaccaccac ctgatgaagg gccaccttta cctgatgtac cgttcacatt cccactggcc
     7261 tgaccaccat tgtgtcccag acctgaatta cccggacctc gtccgtctcc gccactcata
     7321 tacttttcct ctttgacttt taaaacaata aactaaaata aaatactgta catataacca
     7381 ctggttttat atacagtaca aaaattacgc tgccttgttt ttcatgtcaa gtctgtaaat
     7441 ttgcgttttt gtgaaaatga tcaaaaaaac agccactcac accatgcgca atcccctcca
     7501 gatgcaacaa aaaccagcac aaacggcgcc gttctccggc ctcagtatgt tttactgaaa
     7561 tcattatatg agataccaga aagccccctc cgggacagga aaagaaacgg ggggtctggt
     7621 tcgggttgtt cggtcactga acacggtcag aaactgatga taagatagcc tgcggatata
     7681 taactcaatg agaaaaaaga attaaaggta aggagacgct catgagacgt tccggcagca
     7741 aactgtgtct tatcgccatg ataacagcga cagcaactct cactgcctgt gctccaaagg
     7801 gcagcgtgga acagcatacc cggcattacg tatatgcttc agatgacggt tttgatccca
     7861 atttttccac ccagaaagct gacacaacac gaatgatggt gccttttttc cggcagttct
     7921 gggatatggg cgcaaaggat aaagcgaccg gaaaatcacg gagtgatgtg cagcaacgca
     7981 ttcagcagtt tcacagtcag gaatttttaa actcactcca aggcacaaca caatttgcgg
     8041 gtaccgatta ccgcagcaaa gacctcacac cgaaaaaatc aaggctactg gctgacacca
     8101 tttcagctgt ttatctcgat ggctatgaag gcagacagta agtggattta ccataacgcg
     8161 tcattacgcg caccgctcag acgggctcag ggcgtttccg gcggctacgg gataaaacag
     8221 gcaacaaccc ccccggaaaa ctgccggaaa cgcgcccgat aaattttatc ctaatgcatg
     8281 cctatgcacg caactgcatg gcatatgttt tgacgtaaaa tggatgccgt aagccctttt
     8341 ttaccgactt tctgcttcat cagcctgact gacacgacca tattctttca gaccgccccc
     8401 tgctaaactt ccttcatatt gccgcattaa cccgggaggc tccgcaatga gcaacactac
     8461 acactacgaa aacgccaact ttcttcggga actggctgaa agcctgcccc gcatactgcc
     8521 ggaaggtggc ccggacaaag ctgcgctgtt gcagcgactg gctaacgaag aactggccca
     8581 ggcagagtat gaagatcagg ttcgcgccaa agtgaccgcc gcccgtgctg acacgcgtcc
     8641 gggtatgacc acggaacaac tccgccagcg cctgcatggc cgttatcagg aactgcgcga
     8701 tgccgtatga cgtggaatgg aagcagggag ccatcgagga tgtaacgacg ctgtttgact
     8761 atatcgcgga aaattcatcc ctgtgggatg cgcagaacgt taccgaccgg gttctcgctg
     8821 cggcggacaa actggccgac ttcccgcgac tctatgaagt ggactcacgc tatggcgagg
     8881 acgttcgccg gatcagtctg atgggacagc atgtcctcta tgatgttgat gaccagacca
     8941 gaaaagtccg ggttctggcc gtggtcggcc agcgccagaa tccccatatt gttctctaat
     9001 aaccggtgac ggggacgcaa ttcattgcgt ggccgtcgcc ggttggacaa gcacagcgcg
     9061 tcagcgtttt ccccacggcc accaccggcg cggtgttccg gccggggtct ctgctgtctc
     9121 cggtttgtgt tccagcagtc tcatggactg catttcctgc cgtagcccgg ccacctcctc
     9181 cctgagtgct tttatctccg cgatcaacgc gtcggaggcc attttttcag tctcacccga
     9241 cagtctcaac tcgtcccgct cgtgagtctc atcctgtctt aactcaccgt acacccggat
     9301 aagctcgctg gtatccacca cccggccgcc atcagcttcc gtacggtatg atacgcggcc
     9361 tttcgccata tcccgataca gggaacttcg ggctttaccc gtcagttccg tcgcctgtct
     9421 caccgtcacc cacgccattg tctcaccccg ctatttttga gacagtatac ctgacaggcg
     9481 cacaccatgc ccggaaggaa agaatc
//
