LOCUS       CP067662                3991 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-4408 plasmid pC4698_2, complete
            sequence.
ACCESSION   CP067662
VERSION     CP067662.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868505
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C4698
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:16:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,541
            CDSs (total)                      :: 5,417
            Genes (coding)                    :: 5,319
            CDSs (with protein)               :: 5,319
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 43 of 98
            Pseudo Genes (incomplete)         :: 45 of 98
            Pseudo Genes (internal stop)      :: 21 of 98
            Pseudo Genes (multiple problems)  :: 10 of 98
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4408"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC4698_2"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            join(3878..3991,1..159)
                     /locus_tag="JJZ87_27660"
     CDS             join(3878..3991,1..159)
                     /locus_tag="JJZ87_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMF15753.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            740..1714
                     /locus_tag="JJZ87_27665"
     CDS             740..1714
                     /locus_tag="JJZ87_27665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMF15754.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            2461..2652
                     /locus_tag="JJZ87_27670"
     CDS             2461..2652
                     /locus_tag="JJZ87_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMF15751.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2652..3074
                     /locus_tag="JJZ87_27675"
     CDS             2652..3074
                     /locus_tag="JJZ87_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UMF15752.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1942..2413)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 gggtatgacc ggaaaggtaa aatacaggtc accacgggat gctgtgccgg aatgctgaca
       61 gagccggttc tgctgggtgt ctgtgggtgc tttgacccgg aagaacgtga cgagatcatg
      121 cagaatcacc ccatggcaaa gcagtttttc acagagtgat tccgtatctg ttgagtctga
      181 agcccgcctt gtgcgggctt tttcatatct gcgtgaaatg aggcgttatg gtaaatgcac
      241 gcattcattc ttttgaaaaa tccagaatga cgaatacatg gggcatgttt atatgactat
      301 tgagaggaag gatgttattg taaaaaaatc ctttaaattc aatcatttaa ttatggtagc
      361 caaaggggcg gggcttcgct tattccttag gaataagcga agccccgccc ctttggctac
      421 cagtgcatta tacttttttt ttcacccaat gtcaatacat gggttgattt tatcatttta
      481 agcatttttg ttgttgtgag tgctgggttt atgccctata ttcagcgcta agcaagcatg
      541 ggcttgcaaa ggaacaatta atgagaggtg ctctatgaaa aaaatcaaac ttcacgatga
      601 gcgtccagac ttgtaagtgt caagtcagca gaaaaaggca gccaaggctg ccttttttgt
      661 tattggttag tatatgattg agcttatctt aacttacctg aacaaagttt tattgtttgc
      721 tttgcgcaag gattctctca tggctttttt taacctattg tttgttgcat ctctaatttg
      781 ttttggtggt gttatgggga gctattccag aggttgccga gactcccaaa ataaattttc
      841 aaaagataag gatgagaata ataaaagagc tagtgtttat agatttggta tagcatccgc
      901 atttatttgt gttcctttca tatcatcatt tcttcatgtt gattactcaa gtataatatt
      961 tcctgtggct gatggtggtg gaactaagtt catagaacaa atattgcttc ttatttctgt
     1021 ttcggggatc tctgcgtatc ttgggtacgc attactggat ggtcttgcca acaaagtatt
     1081 aaaagagcaa gtagacggta tagataaaaa gcaacaagat ttggaggctg aacaagatga
     1141 gtttaaagat gagcttgata ggagcaagga gctgatagaa cagcttgaaa tggataagaa
     1201 aacaacaaaa ttcgagctag gatactttaa agccatttca gctgtggata aagcagaatc
     1261 aatgatgtcc attccggatg aggctctttc tgttaagaaa aaactaactg aggcattaga
     1321 tgctgtaacg gaatcattgt ctcttgttaa aagagaggat gtagctaagg atgactacga
     1381 caagctctta gtgcttaaag cttatatttt gaaaagactt gatcgtattg atgatgctct
     1441 gtcaattacg gatgagttac ttatgagtaa tgaggataac cctatactta tttacaataa
     1501 ggcttgctat caatatattt tgcgtcgttg ccaagctgat aactccgata ttaaggatat
     1561 gattcgcagg gctctgacta ttaaagttac agatcctgaa ttcattcgca ggcaagaaaa
     1621 aataagaacc aaggtcatag gaaataaaga taatgatctt gaagggttgt ttactgacgc
     1681 tgagctggag gaattaaagg tggctatcaa ataggcagtg gctagctcta tgaattccaa
     1741 aaagatcctt gacgatcctt tgaggtgcag cgtactctac ccctcaaacg aaaaaaccac
     1801 ccggcaaggt ggtttttcga aggttcagta accggcaagt ttctgaaccg aggtaactgg
     1861 cttagaggag cgcagtcacc aaaacagttc gtttagttta gccttaactg gcgcataact
     1921 tcaagactaa ctcctctaaa tcaattacca gtggctgctg ccaatggcga taagtcgtgt
     1981 ctttccgggt tggactcaag agatagttac tggaacgggc gcagcagtcg gactgaacgg
     2041 ggggttcgtg catacagtcc agcttggagc gaactgcctt cccggagccg agtatcaggc
     2101 atggaatgag aaaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     2161 caggagagcg cacgagggag ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg
     2221 gtttcgccac cactgatttg agcgtccgtt tccgcgatgc ttgtcagggg ggcggagcct
     2281 atggaaaaac agccgttcgg cggcctttgc ttttctgcct catatcttgg ttgcctacgt
     2341 ttttatgtac acgctcctgc cgctcgccgc agccgaacgg ctggagcgta gcgactgagt
     2401 gagcgaggaa gcggaaaaga gactagtttg acattgagca ctgacgctct gaggcttctt
     2461 atgacaaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg
     2521 ctcctgctgg ataagcttaa cgagcttgat ctcgatgtcg aggccgacct ttgtgagaaa
     2581 ctccatgatg atgcggagca tcttttccgt acactgtcct cgagactgga tgaattacag
     2641 gatggtaact gatggccata gtgtctatta gtgaagcggc acggcttgtt aaggttagtc
     2701 gccccacaat ttacaagatg attaacagtg ggaagttaag ttacacctct gtggtaaagc
     2761 acgggaaagc tattaaagtt attgatactt cagagctaat tagagtgttt ggttctcttg
     2821 atggtgtaat cgatactgta aaatatgatg taaaaagtga cgcagaatct acaggtatta
     2881 acagcgttgg tttacatgat ttacaacata gaatcgcttt acttgaagct gaaaacgatg
     2941 gtcttaaagg ggctgttaag gctcgagatg agcatattga ttcccttcgt caggctatgc
     3001 agcttctgga gcacaagcac gaaccttctt caccacccca ttccccgtgg tggaaattct
     3061 ggaaaaaatc ctgatcccgc catgcttcca ttttgctctc cggccattcc ggggagttcc
     3121 tgaaccttcc caaaaatcgt cagatccctg aacgctcctc accactaaaa atacccacac
     3181 actacgcaaa attaaaattt tgcagataga cctttatttt caatcagtta tctattttaa
     3241 attacgcaat tttatttttt gtaaggtgaa tccgagtcgt ggacgactct atggcgaatc
     3301 gccgatgact tgcccacgat tcatatgtga atcacgccat ataatcagta cgttatgatg
     3361 acttgactct agagtcgact tgccgggttg gtggcttttt gaccatggcg actctagagt
     3421 caagtcacga gtcgaatcgc cggtgaatcg tgtggtgagt cgtccggcga cttgccataa
     3481 tgtgattgct ggtctcgaca aacccgattc ctgaacgtgc tgaaaaccgg ttttccggct
     3541 gcgccggcaa cgcttcacgg ccacgcaata aattgcgcac ccgttcaccg ttttcagtct
     3601 ggtttggtgc gggttttgag ttgcctggcc tgaattcagg ctgaaatccc cagacagtca
     3661 gtcaagtgca taacgattca tgcccgtaaa acgccctgga gcgattttga ggcgttttca
     3721 gggggtaaga cgtaggattt aatgggttac gccccagaat cgttctgagg ccgttttagc
     3781 ggtgcgtgta atgggacgtt atggtaaatc aacggcggat gcctgagtct gtgttagagt
     3841 cagcggattg tgataatcag tgactcaggg agatgttttg gctactgacg ctcaggtaaa
     3901 agaaattaat gcactcatca aaaaataccc ggactcatgc tccatctgtc atgaagtcta
     3961 tgatgaggat gatgtgacct acaccgtttt t
//
