LOCUS       CP067621                5584 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-4861 plasmid pC592_5, complete
            sequence.
ACCESSION   CP067621
VERSION     CP067621.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15869036
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C592
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 165x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:09:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,902
            CDSs (total)                      :: 5,777
            Genes (coding)                    :: 5,658
            CDSs (with protein)               :: 5,658
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 45 of 119
            Pseudo Genes (incomplete)         :: 71 of 119
            Pseudo Genes (internal stop)      :: 18 of 119
            Pseudo Genes (multiple problems)  :: 15 of 119
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4861"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC592_5"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            79..324
                     /locus_tag="JJ513_29465"
     CDS             79..324
                     /locus_tag="JJ513_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UME67325.1"
                     /translation="MRETCLDTRPARSLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQ
                     WSGADRVQVVAALMAIDAGLERLRHAVLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            263..1027
                     /locus_tag="JJ513_29470"
                     /pseudo=""
     CDS             263..1027
                     /locus_tag="JJ513_29470"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1002..1511
                     /locus_tag="JJ513_29475"
     CDS             1002..1511
                     /locus_tag="JJ513_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UME67321.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1518..1730
                     /locus_tag="JJ513_29480"
     CDS             1518..1730
                     /locus_tag="JJ513_29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UME67322.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2397..2621
                     /locus_tag="JJ513_29485"
     CDS             2397..2621
                     /locus_tag="JJ513_29485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="UME67323.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="TraY domain-containing protein"
     gene            2605..2871
                     /locus_tag="JJ513_29490"
     CDS             2605..2871
                     /locus_tag="JJ513_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UME67324.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2941..3291)
                     /locus_tag="JJ513_29495"
     CDS             complement(2941..3291)
                     /locus_tag="JJ513_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UME67318.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3357..3902)
                     /locus_tag="JJ513_29500"
     CDS             complement(3357..3902)
                     /locus_tag="JJ513_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UME67319.1"
                     /translation="MFFKRYLTEKEIYKVALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3928..4309)
                     /locus_tag="JJ513_29505"
                     /pseudo=""
     CDS             complement(3928..4309)
                     /locus_tag="JJ513_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198370.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            5399..5575
                     /locus_tag="JJ513_29510"
     CDS             5399..5575
                     /locus_tag="JJ513_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UME67320.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSV
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          5137..5307
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.091"
                     /coverage="100.0"
                     /evalue="1.64e-38"
     Typing          complement(4666..5156)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.481"
                     /coverage="81.0"
                     /evalue="0.0"
     Typing          315..1846
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttaggta tccgggtgac cgatggggag taccagcagc tgctggagcg ctgcaatgga
       61 aggcaactgg cggtgtggat gcgggaaacc tgtctcgata cgcgcccggc gcggtcgttg
      121 cggctgccgt ccatcgatcc ggttttgctg cgccagcttg ccgggatggg gaacaacctc
      181 aaccagatcg cccggaaaat taacggcggc cagtggtcag gcgctgaccg ggttcaggtg
      241 gtggccgcgc tgatggccat cgatgccgga ctcgagcggc tgcggcatgc cgtgctggaa
      301 aagggggcag acgatgatcg ttaagtttca tccgcgaggg cgaggcggcg gtgccgggcc
      361 ggtggattat ctgctgggga aagaccgcca gcgcgacggg gccatcgtcc tgcagggaaa
      421 gccggaggag gtccgggagc ttatcgacgc ctcgccctac gccaaaaagt acacctccgg
      481 cgtcctgtct tttgctgaac aggatttacc gcccggccag cgtgaaaagc tgatggcgag
      541 cttcgagcgg gttctgatgc ccggactcga taaagaccag tacagcgtgc tgtgggttga
      601 acaccgggac aaggggcggc tggaactgaa ttttctggta ccgaacacgg aactgctgac
      661 cggcaggcgt ctccagccgt actacgaccg ggcagaccgt ccgcgcatcg atgcgtggca
      721 gaccgtggtg aacgggcgac tggggctgca cgacccgaac gcaccggaga accggcgcgc
      781 gctggtgacg ccatcttcgt tacccaaaac gaagcaggaa gccgcagagg ctattacgcg
      841 gggcttactg gccctcgcct cgtcagggga gcttaaaacg cgtcaggacg tcactgaggc
      901 gctggaaagc gcaggttttg aggtcgtgcg caccacaaag agcagcatca gcattgccga
      961 cccggacgga gggcgaaaca tccgacttaa gggagccatc tatgaacagt cttttaacgc
     1021 tggcgaagga cttagagcag aaatcgaaag cgcagcagca gagtaccggc gaaatgctga
     1081 aagccgcatt cagcgagcac gagaagtctg tcagagcgga actgagcgaa agcgggaaga
     1141 gaatcagcgc cgccatccgc gcccacgagc agggtatgag cgcggccatg cagtcgaacc
     1201 gcctgagcgt gatgcgtatg gtcgggcgga catggctgac catcacgatg gtgtcaggac
     1261 tgctgttcgc cagcctgagc ggggtgttgt ggtatcaggg gagcctgata gcgtcaaatc
     1321 tggccgaaat cgaccggcag aacgcggcgc tgtcgaagct gaacgcgaag acctggggcg
     1381 tgacgtacct ggaagacagc aacggacgtt ttctggtgct gccgaagggg acggccgtag
     1441 acaggacgca gagctggacg gtcgggaacg ggcggtcgaa gcagaacgcg ctgagactgg
     1501 tgaaggagta agtgccgatg accgagctgg aaaaacagtt gctgagcgca ttagagcagc
     1561 tacagcagga ctactcgaaa aggctggacg agtgggagag cgccttcggg gaatggcgga
     1621 aaatgtcagg gcttatgcaa cgggagaacg cggcgctgag cgagcgcgtg acgcgcttga
     1681 gtcagcaggt ggaacgcttg agcgggcaac tgcagcgttt gagccggtag tgcagcgtca
     1741 tgagatggcc gtggccgcag aacgggcgca cgagcaacgg cagcacgaaa aggaactgac
     1801 ggaagcgcgc tcccggcagc gaagctatga cggtccgtcg ctgggctgaa atgcaggaaa
     1861 acagggttct catcgggcat ttacgctgaa accggatgtc attcacctgg ctgcatggct
     1921 atgcagccag gtaaaattta tctgtcgcga acggggcggt ttatcgggtg gtttgttgcc
     1981 ggttttacct gactgccacc ccgttcgcgg cgaacccgtc cggggcggtg cgggcaacgg
     2041 gtgttatgtt taatcagcgt gaacggcaga tacaaaaaag ccccggaggg cttttaacta
     2101 ctgacttatt tttttgaaga tttgctgggt ttcaatttgt cagtatttcc cggcgttttt
     2161 ttattgtcac gctggcgttt atcaggttcc cctgttgaat cggcaattcg tttcggaccc
     2221 ggcttaagac ccataatcgt tatcctttca atagttaaga ggaaagccct caagctagat
     2281 atattgaccg cgttacggat tatcaagagg ggctaacaag attatttttt gttcttttag
     2341 gttgattgtg gtactgttga tatacatgta tagcaaatgg tgttcgggag gtttatatgc
     2401 ttgctatccg gttatctgat gagattgagt cccgtctgga ctcgctggcg aagcaaaccg
     2461 gcagaacaaa gacgttttat gcgcgggaag caatactggc gcatctggaa gacctggagg
     2521 attattatct ttcagcagaa actgctgcac gcgttcgccg tggtgatgaa gcagtgcatt
     2581 cgtctgaaga cgtgaggaag tcacttggtc tggacgatta actattccga tcgggcgctc
     2641 aaatcgttac gcaagatgga caaacagaac gcacgacgga ttgtggattt tatgagttta
     2701 cgcattgcag ttgctgccga tcctcgccag tcagggaagc cgctcaaagg tgagctgggc
     2761 gagttctggc gctatcgggt gggagattat cgcgttctgt gtgagatccg agatgacgag
     2821 cttgttatcc ttgccgccac gattggacat cgccgcgaag tttacgactg aagcccgcta
     2881 tgcgggcttt tttgatgccg acaacaggta ttatggtaaa tactcgacta actacgtgtt
     2941 ctactgtttg tcggttttag ttaaccagca ggttgtctta acgataatgt agccgttatt
     3001 tttaactaat aaggtcatat tgttaacgtc aggcataata aaagacatta tggcctcaga
     3061 gttatcatca gaagcagctc tctgcattac tacttcgatt cccttttctt tgaatctata
     3121 agtatccagt tgcctattaa acttagaaag acctacaacc atacttagtt tgttatcttt
     3181 aacagtaaga gatgcttcct gagtaccatt acatcggtag taagcggtgt aaccgtcagc
     3241 aaaagatcga ccggggaata taaatgctag gaacgcaatt aaccaataca tagttgagaa
     3301 tcttggtatt acacatttca ctatcctctc tttcattttt cgttcaccat actttgttat
     3361 ctgattttta atttttctat tttataaacc aagtccctac caaaaaggcg aaaattatcc
     3421 tcaaataata ataattgttt aagaacatcc atgaaagcag ctgcttcacc ctcagttatg
     3481 ggagctactc tatataacat ataacctgag ttaagggttt cttttatact ttcttcaatg
     3541 ctcattataa atattagtgg attatcattt ttcttccccc atgggtttct aattaatcct
     3601 tcgatgtact caggtccttt gcgtcctatt gcctgcgcaa tgttttttat ttcatctgcg
     3661 tgagggcttt gttgtacagc tttgtttatg cctgcttgag tgattttcag agactctaca
     3721 ccgtgtatgt tgtatgagat aaaatgtaat aaattaagat gtaatgcaat taatccattt
     3781 ttgtttacat ctgagtattt ggataaaaaa cgtaatgaag tgttaactct cccttgaagc
     3841 ctaacaattg ggagtgctac cttgtatatt tccttctccg ttaaataccg tttaaaaaac
     3901 ataattctcc gcctccgtat agcgtgccta ttttatttcg tcgtaaaact tagctctgaa
     3961 atattccttt agaaccatca tttcattaat aattgaatct atctcatcca ttttttcttg
     4021 gtattctttt aatggtgaag ggagcggtaa ctcaatattt gaaaagtgat tggcgagata
     4081 gggatcaagg tttctcatcc attcaggctt ttcgccaatg cgttcttcaa ctgaaagtga
     4141 acctgataat ctctggttaa taagatcact tagactcatt ttggttttat tcgaaagccg
     4201 ttgtaaggat tgctttaatc ctatatctag catgatattt gttctttctc tcattacccc
     4261 ccccctcgct attgcgtttt ttgttgcgca caaagctacc attgcgcatt aagaaacgca
     4321 ataaaaaagg agggagggag tggtttgtac atgatgtgca gtgcgctgag ggcatcagtg
     4381 gcgggtgttt ttccgtggga tggatggagc tccggggata acagaactga cgatcattta
     4441 aaggatcttc ttgagatcct ttcaggagcg tcgtaatctc ttgctctgaa aacgaaaaaa
     4501 ccaccctggc aggtggcttt ttcaaaggtt aaaaaatcct ggcagatctt ttaaccaagg
     4561 taactggctt tagtggagcg cagataccaa atctgtcctt tcagtgtagc cgcagtcagg
     4621 ccaccacttc aagactctcg atatctaaat ccactaattc ctggttacca gtggctaccg
     4681 ccaatggcga tttgtcgtgc ctttctgggt tggactcaag acgatagtta ccggaaagag
     4741 cgtggtagtc ggactgaacg gggggttcgt gcatacagtc cagcttggag cgaactgcct
     4801 acccggaact gagtgtcagg cgtggaatga gataaacgcg gccataacag cggaatgaca
     4861 ccggtaaacc gaatggcagg aacaggagag cgcacgaggg agctaccggg gggaaacgcc
     4921 tgggatcttt atagtcctgt cgggtttcgc cgccactgat ttgagtgtca tatttcgtga
     4981 tgcttgtcag gggggcggag cctatggaaa aacggcttcg ccgcggcctt ctcatttccc
     5041 tgttaagttc cttcctggca tttaccagga aacttccacc caattcgtaa gcccgcaccg
     5101 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaatatctcc
     5161 tgtattgcat cttctgctga cgcgccggtg ccaccttttt ctcctgccat atgaagcact
     5221 ttcataaagt gctactccgc cgtaacatat tgagccagta tacacagcgt aaacgctgtg
     5281 actggttcag ggctgcgccc cgaaacccgc taacaccact gacgcgctgg cgcttgtcca
     5341 acgccggcgc gtcccggaaa acatttccgt gccgtccggc gtttgcgaag gagggataat
     5401 gcagaagccg aagaagaagc cgctcgtcgt aaaaaaaaga tcctggcatc cggcagtgat
     5461 ggcaaatctg atgcgtcagg cacaggagga agattaccgc cgggagcaga tggcagagct
     5521 tgagcgttca gtccggtcac attttgcgga tatcggagga gaggatggcg gttaagcgta
     5581 cccg
//
