LOCUS       CP067562                4167 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-4568 plasmid pC685_6, complete
            sequence.
ACCESSION   CP067562
VERSION     CP067562.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15869050
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C685
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 158x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:14:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,641
            Genes (coding)                    :: 5,497
            CDSs (with protein)               :: 5,497
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 59 of 144
            Pseudo Genes (incomplete)         :: 80 of 144
            Pseudo Genes (internal stop)      :: 28 of 144
            Pseudo Genes (multiple problems)  :: 20 of 144
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4568"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC685_6"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            1..192
                     /locus_tag="JJ527_28795"
     CDS             1..192
                     /locus_tag="JJ527_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMD86510.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            192..593
                     /locus_tag="JJ527_28800"
     CDS             192..593
                     /locus_tag="JJ527_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UMD86511.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(939..1181)
                     /locus_tag="JJ527_28805"
     CDS             complement(939..1181)
                     /locus_tag="JJ527_28805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMD86512.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(1547..2179)
                     /locus_tag="JJ527_28810"
     CDS             complement(1547..2179)
                     /locus_tag="JJ527_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMD86513.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="hypothetical protein"
     gene            complement(2179..3294)
                     /locus_tag="JJ527_28815"
     CDS             complement(2179..3294)
                     /locus_tag="JJ527_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="UMD86509.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="AAA family ATPase"
     gene            complement(3639..3752)
                     /locus_tag="JJ527_28820"
                     /pseudo=""
     CDS             complement(3639..3752)
                     /locus_tag="JJ527_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(3640..4047)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg
       61 ctcctgctgg agaagcttaa cgagcttgat ctcgatgtcg aggccgacct ttgtgagaaa
      121 ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga tgcattacag
      181 gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta
      241 gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgagcgca
      301 atgcaaaaat ggtagatact tcagagcttt tacgtgtctt tggccccttt gagcaaccga
      361 agggtgaaca ccattctgga caggtctctg gacagcgtga tacagcctct gctgaccaga
      421 gtgaacaggt gatccgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt
      481 ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg
      541 caaaattaac tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc tgaccccgtc
      601 atgcttcagt tttcctctcc ggccatccca gggagttcct gaaccttccc aaaaatcgtc
      661 agatctctga acgctcctca ccactaaaaa tacccacacc ccacgcaaaa ttaaaatttt
      721 gcagattcct tatttacaac aatgggttaa tgaaaaataa atgtgcggtg atgtgttaca
      781 aagtgcagat ccaagtcgtg gacgactcta tggcgaatcg ctgacgaatt gcccacgatt
      841 catatgtgaa tcacgcttta ttatcatatt gttatgatga atcgactctt gattcaattc
      901 atcactttgg gtgttttttg ctcatggtga ctcttgattc aattcacgat tcgaatcgcc
      961 ggtgaatcgt gtggtgagtc gtccggcgac ttgccataac gtgattgccg gccgtcgacg
     1021 aacccgattc ctgaaggtgc tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg
     1081 ccacgcaata aattgcgtcc ccgttcaccg ttttcggagg agaagaacgc cggaaacagg
     1141 acttcccctg gctgaatggt gtgaatttcc gcgctatgca cttgcgcgca tactcatgca
     1201 tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt
     1261 taccggtttc ccgtcagaaa cgtcctgagt ccgttttagc ggtgcgtaca attatgattt
     1321 tatgttaaat tgttttaagt gaatagagag aatataactt aagggagcat cttgtgctgg
     1381 gtcttttaca ttagatctgc acaatcattg ttttggcatc tggtgatgtc tttaggacgt
     1441 ttgagattgc tgataactaa agtgtatgat gctttctata cgttatccgg cgtttaataa
     1501 caccctggtg ataagccagc ccgtgaaggg ctggcttgat ttggatttat atttttgaaa
     1561 taaaatcttt tatttcatca aagcaattgt ctgctgtgtt tattattcca tttttcatgg
     1621 atattcctat tcttgagtcg gattctctat tgcagctaag atagacttgt accttagctt
     1681 ttttttcgtt tgttggataa gtgtactctt tgtcactcgc tgagatgtca tctttattat
     1741 ttttaatttt ttcgaaaaat tctattatgt attcatataa cgctcgagtg cttgcgtgtt
     1801 caattatcag atcttcaatg gcaccttgct tgtcttttcc tggcattata aagaaagaaa
     1861 tttttgtatt gccattcata gctatctcgt tatgcgagct gggggaggag atttgtagtt
     1921 ctcgcaatgt ggcaattatt gatctttctg tgtcagaaaa tgatgtgtca gcatctaata
     1981 taataaaaat actttctttt tcatcgaaag aatcaatgtt tttgaatgct ctaagtgctt
     2041 gttttaattt tgattttcca tttatttttt cgacaaaaac atcggtaatg ccttgtattt
     2101 caagtaggtt gcatagaaaa tgaaaatcat catcaccctc aacgaaaatg atctttttgc
     2161 tattatttat gtctttcatt atcttacctc ccagtcgttc tcaactgagt aggagaaatc
     2221 atccatattg aattgtacgg ctgttggttt tagggagttc ttttctcttc ctagtctaat
     2281 ataagcgata tcttcatttt tgctatctat ttcgtttatt gctcgtatca catcttgact
     2341 atgagttgtt gcaaatattt gattgttgtt ttgttttgca gatgaaatta atgattttat
     2401 tatgtcttta attactgagt aatgtattcc gttctcgatt tcatcaataa gaataattga
     2461 gttttcctgt accagcactg agctgataaa agataatgct cgactgatac cctcgccaag
     2521 cattgatatt tcatttagct cactaaatcc aatgtctaaa tatatttcct tattgttacc
     2581 tatcgcagat attgctatat ctacaattct gtcatctatt ttttttaagt tttctagtat
     2641 gtcttttttc cttttttgtg ttagtaggct agatacattt gtaattgttg cctctttgtt
     2701 tatctttctg gatgttgtta ttattgttgc tgtcttaaat ttataattaa tcccatccct
     2761 ttcgtttttt atttcggatt ttatttctac gccatcttct ttcaattgca tagagaaaag
     2821 cttattggaa accctgtttt tcgcattggg tgtctcggtc acatttattt ttaaagagtt
     2881 tactgtggcc tgagaaccga tagtttgacg aggtatgttt tggttagata gtacggtgtc
     2941 aacgtttaga gacaaacttg attctaatct ctgatttgtt tctgtttcat atgtttgtgt
     3001 tacttcagag cgttcatctc gaatgactat ggacatcttt tgtgatgtgt ccatatcatg
     3061 aaagtaacta acccaaaatg gcttgttttt gtttatttct gccatttctt ttctgaatgc
     3121 ttgaatgttt aacaaaacag ccgggttttt tacatcataa aaacaaaaaa tagcatccag
     3181 tatagatgtt ttcccgtagt tgtttttccc agctatgata tttaaagtct ttaattgctt
     3241 tagatatagc tcattgaagc atttgaagtt ctttaaactt atttccttta tcatgatagc
     3301 ctctgttcgt gtttttgtta tatgccatac gatttgttag taccttaata aaatgtacag
     3361 ctttcattgt gattagctag actttatgtc aaagattaac tttgattgct tgtgttttaa
     3421 tcgatttttt ttactttctt cttgagatta tctggcagca gccgtaagct tccggctgtg
     3481 aacgaaaaaa accgccttgg cgggcggttt tttcgaaagg ttcagtagag ttggcgctct
     3541 atgaaccgag gtaactggct taaagggagc gcagtcacca aaactgttcg tttagtttag
     3601 ctttaactgg cgtataactt caagactaac tccctaaatc agttaccagt ggctgctgcc
     3661 agtggcgctt tgtcgtgtct ttccgggttg gactcaagac gatagttacc ggattaggcg
     3721 cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
     3781 ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg gaatgacacc
     3841 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccatcagggg gaaacgcctg
     3901 gtatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga ttccgtgatg
     3961 ctcgtcaggg gggcggagcc tatggaaaaa cggccgctgt gcggcctttc cttctttcct
     4021 ccatgcgatt actcggtttt ctcgagcgtt tctacgcatg ctcctgccgc tcgccgcagc
     4081 cgaacggcag gagcgtagcg actgagtgag cgaggaagcg gaaaagagga gatccctaca
     4141 ctgaccactg acgctctgag gcctctt
//
