LOCUS       CP067561                5591 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-4568 plasmid pC685_5, complete
            sequence.
ACCESSION   CP067561
VERSION     CP067561.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15869050
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C685
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 158x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:14:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,641
            Genes (coding)                    :: 5,497
            CDSs (with protein)               :: 5,497
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 144
            CDSs (without protein)            :: 144
            Pseudo Genes (ambiguous residues) :: 0 of 144
            Pseudo Genes (frameshifted)       :: 59 of 144
            Pseudo Genes (incomplete)         :: 80 of 144
            Pseudo Genes (internal stop)      :: 28 of 144
            Pseudo Genes (multiple problems)  :: 20 of 144
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4568"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC685_5"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            1..192
                     /locus_tag="JJ527_28760"
     CDS             1..192
                     /locus_tag="JJ527_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMD86504.1"
                     /translation="MTKQQKTALNMAKFLQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLTARLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            192..593
                     /locus_tag="JJ527_28765"
     CDS             192..593
                     /locus_tag="JJ527_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UMD86505.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1436..1645)
                     /locus_tag="JJ527_28770"
     CDS             complement(1436..1645)
                     /locus_tag="JJ527_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="UMD86506.1"
                     /translation="MKSIKNFVVVAALSMMSFGAFAQSISATASTLDRAEAKIAAQAAE
                     QGVSYKITSAQFNNRVHMTAELTK"
                     /name="DUF1471 domain-containing protein"
     gene            1972..2130
                     /locus_tag="JJ527_28775"
     CDS             1972..2130
                     /locus_tag="JJ527_28775"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMD86507.1"
                     /translation="MRKTTDALIRDLNHNRCLLDRMKREGMDKVSDTVIVERAMIAIIA
                     EVYIRGN"
                     /name="hypothetical protein"
     gene            2391..3347
                     /locus_tag="JJ527_28780"
     CDS             2391..3347
                     /locus_tag="JJ527_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMD86508.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYIFYLKKEYNGEKIY
                     QIRKNKAGSNEFENNISKLLRSGVLSRVEDNDNYHVYPHLIGGFYEVFFVKGSDGAPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRPEQWKSKFYLDFQKENPDIDEA
                     KQFMPRFPRSSEINGMKIIVTQETNYIEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDRLGRHIDKARVGFILDRIIGIDNKKISEWKQESSKY
                     RGSSKVLSSKSPFSPYFDDEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            3331..4197
                     /locus_tag="JJ527_28785"
     CDS             3331..4197
                     /locus_tag="JJ527_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="UMD86502.1"
                     /translation="MEKEINTWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLDSFFNTFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNTSAMKRLESNQSPSVISIDFSFNEKTYDFDFLSLDG
                     DDNNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILD
                     SLLRKSEGKQLDGLLNVKGIRDVEIIERSSQRYRDLSSTVKSLPPFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            4194..4847
                     /locus_tag="JJ527_28790"
     CDS             4194..4847
                     /locus_tag="JJ527_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="UMD86503.1"
                     /translation="MIAFSFSDGGNRCENLDLFSTINCQNDMFGMVIMDGYSCSEGSME
                     FLSDRLGECVFSGDLDKNIIEPCKSVSVKAAVCFLFKDETHLHIYNAGDCRVYNKSGAL
                     LTEDNSVAWRTLKEKGVDNKKIPHLVCYCPDRNYLTSFVDFCSDEITFNHSVFDLNDEN
                     KEILLCTDGFWEHFDEGTVVDLMKDNPESNLAKFTKNIPDNAENHTVCYVRFPS"
                     /name="protein phosphatase 2C family protein"
     Typing          complement(5070..5443)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.021"
                     /coverage="91.0"
                     /evalue="4.5799999999999997e-172"
ORIGIN
        1 atgacgaaac aacagaaaac agctctgaac atggctaaat tcctccagca tcagtctctg
       61 ctcctgctgg agaagcttaa cgagctcgat cttgatgcag aggccgacct ttgtgagaaa
      121 ctccacgatg atgcggagca tcttttccgt acactgactg cgagactgga tgcattacag
      181 gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta
      241 gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgaacgta
      301 atgcaaaaat ggtagatact tcagagcttt tacgtgtctt tggccccttt gaacaactga
      361 agggtgaaca ccattctgga caggtctctg aacagcgtga tacagcctct gctgaccaga
      421 gtgaacaggt gattcgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt
      481 ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg
      541 caaaattaac tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc tgaccccgtc
      601 atgcttcagt tttcctctcc ggccatcccg gggagttcct gaaccttccc aaaaatcgcc
      661 agatctctga acgctcctca ccactaaaaa tacccacacc ccacgcaaaa ttaacatttt
      721 gcggatttgt atttaatatc agtagtttaa gttggtgaaa tttcgttttt taaatttgat
      781 ttatatagat ccgactctat gacgactcgt catagagtcc ccggcgagtc gtcatagagt
      841 cggtaacgac tcaggactat aaaaacaatg agttaataac aacccgactc tagacccgac
      901 tccccgtttg gttgtttttt agacgtggcg actctgggta cgactctagt gcagagtcgc
      961 cggtgaatcg tgtggtgagt cgttcggtga cttgccataa cgtgattgcc ggccgtcgac
     1021 gaaccggatt cctgaacgtg ctgaaaaccg gttttccggc tgcgccggca acgcttcacg
     1081 gccacgcaat aaattgcgtc cccgttcacc gttttcggat gggaagtacg ccggaaacag
     1141 gacttaccct ggctgaatgg cgtgaatttt cgcgctatgc acttgcgtgc atactcatgc
     1201 atgccgtaaa aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt
     1261 ttaccggttt cccgtcagaa acgccctgag gccgttttag cggtgcgtac aatcatgcct
     1321 ttatgttaaa tgacaggcgt aaaaaagccc ccgaaggggc ttaagggttt tcctgttgag
     1381 ggacgaaccg ggccaccttc cggcgacctg ttcggcagct ccctctgcga ccatcttatt
     1441 tggtcagttc agccgtcatg tggacacgat tattgaactg agcgctagtg attttgtatg
     1501 acacaccctg ttctgcggcc tgtgcggcaa tttttgcttc agcacgatcc agggttgaag
     1561 cggtagcgct gatgctctga gcaaaggcac cgaaagacat catagagaga gcagcaacta
     1621 caacgaaatt tttgatggat ttcatggtcg tattccttaa gtcattttgt tctttatggg
     1681 gcgttttttc cctgatgtga taaatgatat gaccaccatg agctaattaa aatcgaaaca
     1741 atttgctgat gtcattcaaa aaaacagaac aaaatatgag catctaaaaa ccatgtgtaa
     1801 tcctctggtt tcagagagca ggatgaatag cctcgagggt tcgtacaact agggcgttat
     1861 ggtaaatagc gccgaaaact catcttttac gcccattatt tagggagggc gtaaaacaag
     1921 ggataaataa gaatgagcca tgagctcgta acaaaaagga gtggaaccct tatgagaaag
     1981 acaacagatg ccctaatcag ggatttaaac cataaccgct gcctacttga tcgcatgaag
     2041 cgtgagggaa tggataaggt tagcgatacg gtcattgtgg aacgagcgat gatagcaatc
     2101 attgccgaag tttatatccg aggaaattga gataagcagg tccgcagaca gcggacctgc
     2161 tttatggcgc tggatgtgag gagtaatgaa aacagccaca tagtaattat tcttgacttt
     2221 ctacattagt cctataaaat aaatccttag gatttatttt ataggacaat acaatctaac
     2281 gcatgtcaac tcgttgttta gcattgattt ttacttgaca aaccatctct aatagaatat
     2341 acgtccttag gacgtatatt ctattagaga ctaaaccgaa aaggttgaaa atgtatctta
     2401 ttgaagaagc gctaacgtct tggttgttga acgaatataa aaaaccagtc ataaccaaag
     2461 aagaaatttc tttgcaaatt tatatctttt atttaaaaaa agaatataac ggggagaaaa
     2521 tatatcaaat caggaaaaat aaagcaggat ctaatgagtt tgaaaataac atatcaaaat
     2581 tattaaggtc tggagtttta tcaagagttg aagataacga taattatcat gtttacccac
     2641 atttaattgg cggtttctat gaggtttttt ttgttaaagg tagtgatggt gctcctgaaa
     2701 acgatatagt gtgttctacg tatccatatg gatatttatc ctatttatcc gctatggatt
     2761 ggcataatat cacagataaa atacccaaga taatccactt tacgacatgc cgtcccgagc
     2821 agtggaagag taagttctat ctagattttc aaaaagaaaa tccagatata gatgaagcaa
     2881 aacaattcat gcctagattt ccaaggagtt ctgaaattaa tggaatgaaa attatcgtta
     2941 cacaagaaac taattacatt gagccaatga aggcgcgtaa ttctcctgtt agggtgtcct
     3001 ctatagggaa gacttttatg gatatgacta gaagacctga ttactgtggt ggtgaggaac
     3061 atgtattgga gtcatttatt gagcacggta aaaaatatgc gcctctgatt ataaaagaag
     3121 ttgataggct tggtcgtcat attgacaagg caagggttgg gtttattttg gatagaatta
     3181 ttggaattga taacaaaaaa ataagcgaat ggaagcaaga gtcgagtaaa tacagaggta
     3241 gttcgaaagt tctatcttca aaaagtccgt tctcacctta ctttgatgat gaatggttta
     3301 tttctattaa tgttgatatg gttaaatctt atggaaaaag aaattaatac ttggatcgaa
     3361 gagtctggcg ggaagtacag gttgatgcgg caggctgtgc atataatact gaaatctatt
     3421 tctatagagg agagtttgaa tgaaatgatg gttatgaagg gaggaaccct tcttggtgtt
     3481 aagcatggta gtgatagata cacaaaagat attgatttct ctaccgaaaa aaaactttcc
     3541 gaaatcgatc tagatagttt tttcaataca tttaatgact caatggatgt tgctgaggct
     3601 gaattgggat atggtattaa atgtaaggta caatctcata aaatccagcc taatgcgcaa
     3661 ggaacatttc caacggtaca gataaatata gccttttgcg atcgatctaa tacgtcagca
     3721 atgaaaaggc ttgaatctaa tcaatcacct tcagtgataa gtatcgattt tagttttaac
     3781 gagaaaacgt atgattttga ttttttgtca ttagatggcg acgataacaa cgatagtgta
     3841 aaaacctaca gcttaaccga tttgatggct gagaaatatc gttctgtttt acagcaaaaa
     3901 gttagagagc gaaatcgtga gcaagatata tatgatatta attatctttt aggtaagtat
     3961 gaattttctc ggcaagataa gtataaaata ctggattcac tcttaagaaa gagtgaaggt
     4021 aaacaattag atgggctctt aaatgttaaa ggtataaggg atgtggaaat tattgaacgt
     4081 tccagtcaac gatatcgaga tttatcatcc accgttaaat cattgcctcc ttttgaagat
     4141 gcttatgaaa aagtcgcctg tttctatgaa agtcttccat gggatatatt taaatgatag
     4201 catttagttt tagtgatggt ggtaatcgat gtgaaaactt agatttattt tctacaataa
     4261 attgtcaaaa tgatatgttt gggatggtta tcatggatgg atattcctgc tctgaaggta
     4321 gtatggaatt cctatctgac aggctaggtg agtgcgtgtt ttctggtgat cttgataaaa
     4381 atattattga accctgcaag tcagtttctg ttaaggctgc ggtatgtttc ctttttaaag
     4441 atgagacgca tctccatatc tataatgcag gggattgccg tgtatataat aaaagtgggg
     4501 ctcttctgac cgaggataac tcagttgctt ggagaacctt aaaggaaaag ggagtggata
     4561 ataaaaaaat acctcacctc gtttgttatt gcccagacag gaattatcta acatcatttg
     4621 tcgatttctg ttctgatgag ataacattca atcattcagt ttttgattta aatgatgaaa
     4681 acaaagagat tcttttatgt actgatggct tttgggagca ttttgatgaa ggtactgttg
     4741 tcgatcttat gaaagataac ccagaatcta acttagctaa attcaccaaa aacatcccag
     4801 ataatgcaga gaatcatacg gtttgctacg ttagatttcc ttcttgattt cgtgtttttt
     4861 gaagaagtta ttgagatctt cgtgtcacat agataatctc ttgtctgtaa aagaaaaaaa
     4921 ccgcctttgc ggggcggttt tctcttaggt tcaacgagtt tgcggctcga agaaccaagg
     4981 taactggctt ggaggagcac agtcaccaaa acttgttctt tcagtttagc cttaacaggc
     5041 gcatgacttc aagactaact cctctaaatc agtaaccagt ggctgctgcc agtggcgctt
     5101 ttgcgtgtct tccgggttgg actcaagacg atagttaccg gataaggcgc agcagtcgga
     5161 ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag
     5221 tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa
     5281 aggcaggaac aggagagcgc acgagggagc caccaggggg aaacgcctgg tatctttata
     5341 gtcctgtcgg gtttcgccac cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg
     5401 ggcggagcct atggaaaaac ggccgccagg cggcctttcc ttccatcatg ctattgctcc
     5461 attttctcga gcgtttctac gtatgctcct accgctcgcc gcagccgaac ggcaggagcg
     5521 tagcgactga gtgagcgagg aagcggaaaa gagaacaccc tacactgagc actgacgctc
     5581 tgaggcttct t
//
