LOCUS       CP067514                4258 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-8045 plasmid pC708_5, complete
            sequence.
ACCESSION   CP067514
VERSION     CP067514.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN17131926
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C708
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 16:15:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,391
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 58 of 147
            Pseudo Genes (incomplete)         :: 90 of 147
            Pseudo Genes (internal stop)      :: 27 of 147
            Pseudo Genes (multiple problems)  :: 26 of 147
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-8045"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC708_5"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            1..192
                     /locus_tag="JJ671_27555"
     CDS             1..192
                     /locus_tag="JJ671_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMD37110.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            192..593
                     /locus_tag="JJ671_27560"
     CDS             192..593
                     /locus_tag="JJ671_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UMD37111.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            1455..2879
                     /locus_tag="JJ671_27565"
     CDS             1455..2879
                     /locus_tag="JJ671_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="UMD37112.1"
                     /translation="MEIEFENIGFIKSGKIKTNNITVIFGPNNVGKTYLSYSTYAAISR
                     YNDIFREKIKIPKSVSSNLVLNGTARYDITNVFTDSLVKSVNEEVSSSLPAFFKDSSGM
                     FSKSKISVIDSYIKDRVVNSEVNVNISVSENIILNIQKKKNNTCIDFTVTSSKKNNESG
                     SPVIDLKEKDASNYLNILFNFFFLNEVLDSAANNAFIITSERTGISLFQKEIDGNRSNI
                     VDSYMWSKLSTRKLLSFDDIMSQKVAKFSEPINHNINIIRDFADLKKMKHIMTANGVRR
                     ASKERTDVIKTLESLTGGRYSTSDGEAIFTVSCSDGDKVSMPLSLSSASNKALFLLDIY
                     VRYYMSNNSYLIIDEPELNLHPHNQIKMAELLVRLSNCGVKIIITTHSDYIVKEINNRI
                     MASQLKDKSRLTELGYKEADLIFSSEVNGFTIGNDGAISEVEGNRYGVNAFLFDNVIND
                     IENKADLLYTYLEEGL"
                     /name="AAA family ATPase"
     gene            2881..3513
                     /locus_tag="JJ671_27570"
     CDS             2881..3513
                     /locus_tag="JJ671_27570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMD37109.1"
                     /translation="MLERFIDVLNKDVLFDLSATPSPDSSVLVVREDGELSEIKELTIK
                     EIPDDSIAFTLDFSSKKNSKENGALFSQLSPYFDKSNGDGVNKSCDLVLITRDGDKFTV
                     LIFDQKSKKPDIESSFLQLENSRVFVSYVINIISLIYKDNIDEGKIVFKRVIGTTRVVK
                     TGVNAANHEQERSRREKLRALGMNEVSIKRTASIEKGWLDYRGIVSF"
                     /name="hypothetical protein"
     Typing          complement(3735..4136)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.611"
                     /coverage="99.0"
                     /evalue="3.5900000000000006e-163"
ORIGIN
        1 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg
       61 ctcctgctgg agaagcttaa cgagcttgat cttgatgctg aggccgacct ttgtgagaaa
      121 ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga tgcattacag
      181 gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta
      241 gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgaacgca
      301 atgcaaaaat ggtagatact tcagagcttt tacgtgtctt tggtcccttt gagcaaccga
      361 agggtgaaca ccattctgga caggtctctg aacagcgtga tacagcctct gctgaccaga
      421 gtgaacaggt gattcgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt
      481 ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg
      541 caaaattaac tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc tgatcctgcc
      601 atgcttgagt tttcctctcc ggccatcccg gggagttcct gaaccttccc aaaatcgcca
      661 gatctctgaa cgctcctcac cactaaaaat acccacacac tacgcaaaat taaaattttg
      721 cggatttgta tttaatatca gttagttact ttcctgaaat ttcgtttttt aaattttatt
      781 tacatagatc cgactctatg acgactcgtc atagagtccc cggcgagtcg tcatggagtc
      841 ggtaacgact caggactata aaaacaatga gttaataacg acccgactct agacccgact
      901 ccccgtttgg ttgtttttta gacgtggcga ctctgggtac gactctagtg aagagtcgcc
      961 ggtgaatcgt gtggtgagtc gtccggcgac ttgccacacg attcaccggc tgtcgatggg
     1021 cccgattcct gaaggtgttg aaaacggttt ttccggctgt gccggcaacg cctcacggcc
     1081 acgcaataaa ttgcgtcccc attcaccgtt ttcagtcggg tttggtgcgg gtttcgagtc
     1141 gtctggccta aattcaggct gaaatctgcc accagtcagt ctggtgcata acgattcatg
     1201 cccgtaaaac gccctggagc gattttgagg cgttttcagg gggtaagacg taggatttaa
     1261 tgggttacgc cccagaatcg ttctgaggcc gttttagcgg tgcgtgtaat gacgcgttat
     1321 ggtaaatcca gtagtttttt gctcattcag gagtggtttt taaatcatca tgtatgaata
     1381 tcttgtaatg gtaggtatag ttagcatgga tattgctaag gataaaagca taacatactt
     1441 agtgggtgat gcgaatggaa atagagtttg aaaatatcgg ctttataaaa agcgggaaaa
     1501 taaaaaccaa taacattact gttatttttg ggccaaataa tgtgggtaaa acatacctaa
     1561 gttatagcac atacgctgct attagtaggt acaatgacat atttagagag aaaataaaaa
     1621 taccaaaatc agtatctagc aacctggttt taaacggaac tgccaggtac gacatcacaa
     1681 acgtttttac tgattctctt gttaaatcag ttaatgaaga ggtttcatca agcttacctg
     1741 cattcttcaa agatagttca ggtatgtttt ctaaatcaaa aattagtgtg attgactctt
     1801 atattaaaga tagagttgtt aattctgaag ttaatgtaaa tatttctgtc agtgaaaata
     1861 taatattaaa tatacaaaag aaaaaaaaca atacatgtat tgattttaca gttacctcat
     1921 ccaaaaagaa taatgagtca gggagtcctg tcattgatct gaaggaaaaa gacgcttcaa
     1981 attatttaaa tattttattt aatttctttt tccttaatga ggttttagac tctgcagcta
     2041 ataacgcatt tataataaca tctgagcgta ctggaatatc cttgtttcaa aaggagattg
     2101 atggtaatcg tagtaatatt gttgattctt acatgtggag taagttgtct acgaggaagt
     2161 tactgtcttt tgatgacatt atgagccaga aagttgctaa gttctcggag ccaattaatc
     2221 acaatataaa tataataaga gactttgctg atctgaaaaa aatgaagcat ataatgacgg
     2281 caaatggtgt tagaagggca tctaaggagc gaactgatgt tataaaaaca ctagagtcac
     2341 ttactggtgg tagatactca acatctgacg gtgaggctat ttttacagtt tcttgcagtg
     2401 atggagataa agtgtcaatg ccactttctc tctcatcagc atctaacaaa gcactatttc
     2461 tattagatat atatgtaaga tactacatgt ctaataactc ttacttgata attgatgagc
     2521 cagagctgaa tctgcatcca cataatcaaa ttaaaatggc agagttgctt gtaagacttt
     2581 caaattgcgg agttaaaatt ataatcacaa cgcatagtga ttatatcgtt aaagagatta
     2641 ataatagaat aatggcttcg cagctgaaag ataaatcacg attaactgaa cttggataca
     2701 aagaagcaga tttgattttt tcgtcagagg ttaatggttt tactatcggt aatgatggtg
     2761 ccatctctga ggttgaaggg aataggtatg gggtgaatgc ttttcttttt gataatgtta
     2821 ttaatgatat agaaaacaag gctgatctct tgtatacata tttagaagag gggctctaat
     2881 atgctggaga ggtttattga tgttttaaat aaagatgttt tgtttgattt gtcggcaact
     2941 ccatctcctg actcttcggt gttagttgtt cgtgaagatg gtgagcttag tgaaataaaa
     3001 gagttaacta taaaagaaat acctgatgat tcgattgctt tcactttgga tttttcaagc
     3061 aagaaaaact ctaaagaaaa tggcgcttta ttttctcaat tgtcgccata ctttgacaag
     3121 tctaatggcg atggggtgaa taaatcttgt gatcttgtat tgattacaag agatggcgat
     3181 aagttcactg ttcttatttt tgaccaaaag tcaaaaaaac ctgatataga atcatcattt
     3241 ttgcagttgg aaaactcaag agtttttgtt tcctatgtaa ttaatattat ttctttaatt
     3301 tataaagaca atattgatga ggggaagatt gtttttaaac gagttattgg gactacaagg
     3361 gttgtaaaaa ctggagttaa tgctgctaat catgagcagg aacgatcgag aagagagaag
     3421 cttcgtgctc ttggcatgaa tgaagtctcg ataaagcgta cggcatcaat agaaaaggga
     3481 tggttggact atcgaggaat cgtttccttt tagtctactt ccagtagtct caagtgcttg
     3541 tgttgagaga ggaagaacgg tatctttcaa catataaaga aaaaccaccg ctaccaacgg
     3601 tggttttctt aaggtttatg gagctaccaa ctcattaaac ctaggtagct ggcttggagg
     3661 agcacagtca ccaaaacttg ttctttcagt ttagccttaa caggcgtcta acttcaagac
     3721 tatctcctct aaatcagtta ccagtggctg ctgccagtgg cgcttttgca tgtcttaccg
     3781 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt
     3841 cgtgcataca gtccagcttg gagcgaactg ccttcccgga accgagtgtc aggcgtggaa
     3901 tgagaaaccg cggccataac agcggaatga caccggtaaa ccgaatggca ggaacaggag
     3961 agcgcacgag ggagccacct atcagaaatg ataggtatct ttatagtcct gtcgggtttc
     4021 gccaccactg atttgagcgt ctttctgtga tgcttgtcag gggggcggag cctatggaaa
     4081 aacgacggcc gtgcggcctt tccttcttcc cgtcatgcgg ctgctcggtt ttctcggcgt
     4141 ttctacgcat gctcctgccg ctcgccgcag ccgaacggca ggagcgtagc gactgagtga
     4201 gcgaggaagc ggaaaagaga acatccttac actgagcact gacgctctga ggcctctt
//
