LOCUS       CP067513                9294 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-8045 plasmid pC708_4, complete
            sequence.
ACCESSION   CP067513
VERSION     CP067513.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN17131926
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C708
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 145x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 16:15:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,516
            CDSs (total)                      :: 5,391
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 147
            CDSs (without protein)            :: 147
            Pseudo Genes (ambiguous residues) :: 0 of 147
            Pseudo Genes (frameshifted)       :: 58 of 147
            Pseudo Genes (incomplete)         :: 90 of 147
            Pseudo Genes (internal stop)      :: 27 of 147
            Pseudo Genes (multiple problems)  :: 26 of 147
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-8045"
                     /isolation_source="other"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC708_4"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            1..180
                     /locus_tag="JJ671_27500"
     CDS             1..180
                     /locus_tag="JJ671_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMD37105.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(208..738)
                     /locus_tag="JJ671_27505"
     CDS             complement(208..738)
                     /locus_tag="JJ671_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMD37106.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(745..1476)
                     /locus_tag="JJ671_27510"
     CDS             complement(745..1476)
                     /locus_tag="JJ671_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="UMD37107.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            complement(1476..3440)
                     /locus_tag="JJ671_27515"
     CDS             complement(1476..3440)
                     /locus_tag="JJ671_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="UMD37108.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4921..5070)
                     /locus_tag="JJ671_27520"
     CDS             complement(4921..5070)
                     /locus_tag="JJ671_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UMD37098.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5155..5412)
                     /locus_tag="JJ671_27525"
     CDS             complement(5155..5412)
                     /locus_tag="JJ671_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="UMD37099.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(5422..7107)
                     /locus_tag="JJ671_27530"
     CDS             complement(5422..7107)
                     /locus_tag="JJ671_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin"
                     /protein_id="UMD37100.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="cloacin"
     gene            7510..7929
                     /locus_tag="JJ671_27535"
     CDS             7510..7929
                     /locus_tag="JJ671_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UMD37101.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8235..8498
                     /locus_tag="JJ671_27540"
     CDS             8235..8498
                     /locus_tag="JJ671_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="UMD37102.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            8488..8787
                     /locus_tag="JJ671_27545"
     CDS             8488..8787
                     /locus_tag="JJ671_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UMD37103.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8849..9226)
                     /locus_tag="JJ671_27550"
     CDS             complement(8849..9226)
                     /locus_tag="JJ671_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="UMD37104.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     BGC             422..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4240..4818
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(749..1476)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atgacaaaac aggaaaaaac agccctcaat atggctaaat ttattaagga gcagtcgctg
       61 ctattgctcg ataaactgaa tgagctggat atggataccc cggccgacat gtgcgagcgg
      121 ctacacgagg atgcggaaac actctggcag gtgatgcggg ataagctcgg ggaggaataa
      181 cagcaccggc atggccggtg ctggcattta ttttttgccg aacggccacc accggcgggc
      241 aggtttgtcc tccggcagtt caggtaccgg cacgttaaaa tagcggctgt tatattcccg
      301 catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cactcaccac
      361 gtcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc
      421 gttctcgatg gcgaaccgct ccaccttgcg atagaggtaa tcacacagct tcattcctgt
      481 gggtttgttc cgttcccatt gcggattttt accagtgaaa aagtacgtgg ctttttcgtc
      541 gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg
      601 gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg agcaccacca tatcttccag
      661 atgctcgctg gtcggcatga tgcagcgctc gccgttccgg tcctccgcgt cttcaataaa
      721 taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc acgcaaacaa
      781 ccgttcacga tccagctctg ccgttttgcg ggcctcgccg gtctgtgggt tcacgatttc
      841 atccaccgac aggattgccc gctgcaccct gacctgcgtc gcccggtccg ggcagatgta
      901 ccagatttca tcgatgccgt tagctttgcg gttgaacaga tacccggaca ccacttcctg
      961 ataccggcga cggctcttga cggtgcgctc cacctcaaac gccaccgttt taccgtccag
     1021 ctccgccagg gcgtccggca ctttcatgcc tttaagactc agacggtgca gaatgcgcca
     1081 gccggtggcc cctctggctt ccatatcaag gcgcagggat tgcacggcca gccggtgcgg
     1141 cagatgtgct gccgacaccc ttgacggctg aaacggaata acatccgcca gcggttcgtc
     1201 ctcgtcgaac gagaacgcca cgccatgcgg cgtcagcccc cacacgctga cctgacggcc
     1261 gtccaccgga tatttggtcc ggaccagcag ccctttcgcc accaggctgt cgaggaagcg
     1321 gtagaccggc tgcaccgccg caatccccag ccgccgcgca accagctccg ccgtggaaca
     1381 cagctcttcc cgcaaccagc gcagggtcag ccgggttttc tccgcgttcc gcgcctgtcg
     1441 tttcgcgtgg ctgacgttgt acagctccag cgccatcagt aaatccccgc aaaatcatca
     1501 tccgctttcg gcccgccgcc gtcggccggg ggccggaact ccgccagggg atcggttttc
     1561 gccagcaccg ggaaacgctg caacgggatt tcccggcgct caacccgcag cggactgctg
     1621 aacgccatcc gggccacacc cagcccggtc atgaccgcga acccgtccgg caggtgttgc
     1681 agggtgttca cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc cccgctgata
     1741 tgctccacgt tccccgcctc ccggctggca cggcggcgct ccacgtccac cctgatttcc
     1801 ccggtctgcc ccgccgccca ctgcgcggac tccaggctgg ccgcccggta aaaccagcgg
     1861 atcggcgtgt tatccagtac cgtggttttc acaaattcag cgggcagatc ctgcccgcac
     1921 tgcccgaaat cgcccagcga ctggtgcgcg agcaacagat tgcagtcccg gctgcgcagc
     1981 gtccccagtg cgttcagtac atacttcgac agcagatatt taatttcatc gagcatcacg
     2041 ctgacgtgtg gccactcccg gaactcgtcc cgcgcaatga tgatttgcgc gcagcgggca
     2101 aacagcattt tttgcacacg gatcaccgcc tcgtcgtcca tcgagccgat aacgtacagg
     2161 cagccgccgc cgttgataat ccccgccaca tcgatgccac tgtccgtctg caggacgctc
     2221 aggtcagcga cgttttccag cctagtgatc aaccccttca cgtccttttt cagggcctcc
     2281 ggcagcgcgt acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg aaactgctcc
     2341 gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc
     2401 tcgcccaggt taagcccggc aatcagcagg tttttcagcg cgtactggtc gagatcgcgg
     2461 aacagattca gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccggacag
     2521 tgcgcccgga agacgtgcgg cgcccatgcg tcccctttgg ggtcgaagta caccaccgcg
     2581 tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg ccatcacgct cttgccgctc
     2641 ccgggcaggc cgaggatctg aatgttggtc ttgcgccagg tatcacgcgg aaccagcacc
     2701 ggcttgccct gctcatcctt acccagaaac accgcctgct cctgccgggc ctgctccgag
     2761 gaaaaatcgg ccagcgcatt aaccggtacc ggctccccga aatgcaggtg gcgcacgtcg
     2821 gtcatctctc ctgtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc
     2881 gcctcgtcag cagccgcaaa atgaccgtca agcagcagga acgccagcag ccgcgccaac
     2941 acaatcccga cagccagccc gcccgcgcac caggcaatcc cgaacaggtt agccggctgc
     3001 agcaggtaat aatctgcgtt tgcgtagagg tccggacgca ggacggtttc tccccagctc
     3061 tcgataaaca cggcgacaac cagccccccc agaagggcca gaccggccgc gccggtcagc
     3121 cagtacgcgt agttccagac atgtcgacgt gccctgacca tccgcccggg cgaatccagc
     3181 gaggccagca ccgaaaatgg cgctggccgt ttattatcgc gcttatagcg atcggcatac
     3241 tcccgtcgct ccttatgact cagccccaca aaacgcatcc gtccggccca aagttcagcc
     3301 agaccgaaaa acaggccgat aactaggaaa gccccggcca gaccgtaata ctcctggtac
     3361 agccagctga cccacggata aatctgccgg gccagctccg gaatgtgcca cttccaggca
     3421 agcgaatccg tattaaacat gttctctctc cttcagacga tggtttgccg gcgtgatgtg
     3481 cagcccccgc tgcacaagcc gcggacatgc cccaaggcat ggcggcggcg gtcacgtccg
     3541 tggtgattgc gttcagcttt caccacggag gccagcctct ttccgcccca tgcggaaaag
     3601 gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag gcggcacccc
     3661 agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga gcggctgacc
     3721 acccgcaaaa tcaggagcag ctttgctgct tcgccaggtg cgtccagcac cgtggatttt
     3781 tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt ggccggcgtc
     3841 cagccggacg gagccagaaa gagggggaga gaccgtgcgt cggtggcgtc cagccagcgt
     3901 aagcagggga tatccccctg aggtcagcca cgccctcgcg gggatgacct gtatttctcc
     3961 gtaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa aaaccgatat
     4021 gattcaccta tcctggtaat ttattatcag ggaatataaa gacttaatca ggggacaata
     4081 tgccacatca gcgcgtccgg tgtccgtctt gtaggtgccg gggaaattgc gcctcctcgc
     4141 tcactcggtc gctacgctcc ggccgtgaga ctgcggcggg cgctgcggac acatgcaaag
     4201 ttacccacag attccgtgaa taagcagggg actaacatgt gaggcaaaac ggcaaggcca
     4261 cgcaggtggc gtttttccat aggctccgcc ctcctgactg agttcacata aacagacgct
     4321 ttttcggtgc atctgtggga gccggtggct caaccatgaa tcagtcagga gtggcgaaac
     4381 ccgacaggac ttaaagatca tcaccgtttc cgatgaatcg ctccctcttg cgctctcctg
     4441 ttccaaccct gccgtttccc ggatacctgt tccgcctttc tcccttgcgg gaagtgtggc
     4501 gctttctcat agctcacact ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
     4561 ggctgtaagc aagaaccccc cgttcagccc gactgctgcg ccttatccgg taactgttca
     4621 cttgagtcca acccggaaaa gcacgcaaaa cgccactggc agcagccatt ggtaactggg
     4681 agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc
     4741 cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt taccacggtt
     4801 aagcagttcc ccaactgact taaccttcga tcaaaccacc tccccaggtg gttttttcgt
     4861 ttacagggca aaagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4921 ctactgaacc gcgatccccg tcagttcaga ggaggacgat ggtgccaccg ttccaccctg
     4981 aacatcccgg atatagtttg cctgacatgc gaccagaaga aagcctgaaa aaatcaatat
     5041 aaaaagaaaa acaaccttta cctttttcat ataccttccc tgtatttaaa aaccccggta
     5101 ttcatccctg aacaccggaa gtctgtattt caaatcatcc ttgataactt ttgcttacca
     5161 gttaccatcc cggtaaacaa aggatacaaa gtaatcaaat ttactaatat cgattacatt
     5221 tttaaagtgt ggctgtaata tcgaaaccca tgatttttca acatcaaacc aaccattatt
     5281 aatattatcc tttaaaggca tccccagact ttcaatgaca gaaccatcat caccgaagtc
     5341 ttttgagtat tcaccacctt taaactcttc ggttttctta tcaaaccaat gaatatgtaa
     5401 tttaagcccc atatttacct cttaaagata ttttttaatg tttcgttttg gatccggccc
     5461 tttaacctgc ttacccgttt ttggatcgaa tgccccgagg tgttcgccat cactggcgcg
     5521 gtacccttca agctcaccgt gctgggaatc ccattcataa attttacgct ttttatcacc
     5581 ataccatcgg gcccgcttac caccaccacc ttgtttaggg gttttaggtt taccctcttt
     5641 caactctccc aaccccttaa tatcttcagt cttgggatca ggaaaataat catggccgta
     5701 atctttagtt cccttacgag gttttttctt ttcctcattt aatttatttt ctgcatcctt
     5761 cgcttttttc tccttttgtt tccggctctc catcgccccg gaaagcgcag ccttatccct
     5821 ggataaggat tcattcattt cgtttaaaga tgtctgggca gcttttaatt tctcattctg
     5881 attctgaata tccttatcaa gatagcgaac ctgatcctgc cacccggagc catattcatg
     5941 aggcgggtta taagtcacaa aatcatcttt atttgcctct gcctctttta cctttttatc
     6001 ggcttcctga acagcagccc ttgcaccggg gatcgccttc tctgtcgatg caatgcgact
     6061 gtttaaggtc gcaatatttt tatcttcagc atccagctca gctttcgctt tatcatactc
     6121 tcttttcagc ccctcttccg ggtgagcagc gtcccatgcc tgctggcgac gcttttcttc
     6181 ctccagacgc tgttttacct gtgccggagt aagaacatcc gtcaccgaca catagaccgg
     6241 tttctgtccg ctctctttcg ggaaacgaat aacggcttca cgggagttac caccggccgt
     6301 aaaaccagcc ggacgtgaat cacctttttc agcaataatg ccttttggcg gggctttcgc
     6361 ggccggaaca cctttaggta cgctcacctg cagagacggg agacccggaa tcgacacact
     6421 gaataccccc ggacgttttg tcggtttcgc atccaccaca ggaacactca ttggccggcc
     6481 ggtgacaacc gcaatatgct gccgctcgtc cttcacaata tccgccacac gtttagtgac
     6541 gctgaccgtc gcctggtcca gcggcaggga gctgaccggc gtctccgtta ccgtgtctgc
     6601 cggcaatgac gtcacgattt ttgacatcat tttcggatca tcttttgcta tctcagaagg
     6661 cagcactccg taaatcgcaa tcccccacag accaaactta aacggacctt tcagggcagc
     6721 cagcacatca gcaacagcgg ccgacaacgc atcgccggaa acggataaag ccagtccttc
     6781 agcccccgga gtggccaagg caggcagacc gaaggccatc gcggtggccg aagactgtcc
     6841 gccaccagag ctcccgctat gattgccgct gttaccgcca ttaccaccgg gtttactccc
     6901 gccgttacca aactcccccg ggttatagtt atgaatatcc ccgttaggtg tatgcgtcgt
     6961 accccagccc ggcccgctgt ttggatccgt accaccacct gatgaagggc cacctttacc
     7021 tgatgtaccg ttcacattcc cactggcctg accaccattg tgtcccagac ctgaattacc
     7081 cggacctcgt ccgtctccgc cactcatata cttttcctct ttgactttta aaacaataaa
     7141 ctaaaataaa atactgtaca tataaccact ggttttatat acagtataaa aattacgctg
     7201 ccttgttttt catgtcaagt ctgtaaattt gcgtttttgt gaaaatgatc aaaaaaacag
     7261 ccactcacac catgcgcaat cccctccaga tgcaacaaaa accagcacaa acggcgccgt
     7321 tctccggcct cagtatgttt tactgaaatc attatatgag ataccagaaa gccccctccg
     7381 ggacaggaaa agaaacgggg ggtctggttc gggttgttcg gtcactgaac acggtcagaa
     7441 actgatgata agatagcctg cggatatata actcaatgag aaaaaagaat taaaggtaag
     7501 gagacgctca tgagacgttc cggcagcaaa ctgtgtctta tcgccatgat aacagcgaca
     7561 gcaactctca ctgcctgtgc tccaaagggc agcgtggaac agcatacccg gcattacgta
     7621 tatgcttcag atgacggttt tgatcccaat ttttccaccc agaaagctga cacaacacga
     7681 atgatggtgc cttttttccg gcagttctgg gatatgggcg caaaggataa agcgaccgga
     7741 aaatcacgga gtgatgtgca gcaacgcatt cagcagtttc acagtcagga atttttaaac
     7801 tcactccaag gcacaacaca atttgcgggt accgattacc gcagcaaaga cctcacaccg
     7861 aaaaaatcaa ggctactggc tgacaccatt tcagctgttt atctcgatgg ctatgaaggc
     7921 agacagtaag tggatttacc ataacgcgtc attacgcgca ccgctcagac gggctcaggg
     7981 cgtttccggc ggctacggga taaaacaggc aacaaccccc ccggaaaact gccggaaacg
     8041 cgcccgataa attttatcct aatgcatgcc tatgcacgca actgcatggc atatgttttg
     8101 acgtaaaatg gatgccgtaa gccctttttt accgactttc tgcttcatca gcctgactga
     8161 cacgaccata ttctttcaga ccgccccctg ctaaacttcc ttcatattgc cgcattaacc
     8221 cgggaggctc cgcaatgagc aacactacac actacgaaaa cgccaacttt cttcgggaac
     8281 tggctgaaag cctgccccgc atactgccgg aaggtggccc ggacaaagct gcgctgttgc
     8341 agcgactggc taacgaagaa ctggcccagg cagagtatga agatcaggtt cgcgccaaag
     8401 tgaccgccgc ccgtgctgac acgcgtccgg gtatgaccac ggaacaactc cgccagcgcc
     8461 tgcatggccg ttatcaggaa ctgcgcgatg ccgtatgacg tggaatggaa gcagggagcc
     8521 atcgaggatg taacgacgct gtttgactat atcgcggaaa attcatccct gtgggatgcg
     8581 cagaacgtta ccgaccgggt tctcgctgcg gcggacaaac tggccgactt cccgcgactc
     8641 tatgaagtgg actcacgcta tggcgaggac gttcgccgga tcagtctgat gggacagcat
     8701 gtcctctatg atgttgatga ccagaccaga aaagtccggg ttctggccgt ggtcggccag
     8761 cgccagaatc cccatattgt tctctaataa ccggtgacgg ggacgcaatt cattgcgtgg
     8821 ccgtcgccgg ttggacaagc acagcgcgtc agcgttttcc ccacggccac caccggcgcg
     8881 gtgttccggc cggggtctct gctgtctccg gtttgtgttc cagcagtctc atggactgca
     8941 tttcctgccg tagcccggcc acctcctccc tgagtgcttt tatctccgcg atcaacgcgt
     9001 cggaggccat tttttcagtc tcacccgaca gtctcaactc gtcccgctcg tgagtctcat
     9061 cctgtcttaa ctcaccgtac acccggataa gctcgctggt atccaccacc cggccgccat
     9121 cagcttccgt acggtatgat acgcggcctt tcgccatatc ccgatacagg gaacttcggg
     9181 ctttacccgt cagttccgtc gcctgtctca ccgtcaccca cgccattgtc tcaccccgct
     9241 atttttgaga cagtatacct gacaggcgca caccatgccc ggaaggaaag aatc
//
