LOCUS       CP067506                3510 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-8050 plasmid pC714_6, complete
            sequence.
ACCESSION   CP067506
VERSION     CP067506.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN17131928
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C714
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 162x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:15:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,953
            CDSs (total)                      :: 5,824
            Genes (coding)                    :: 5,705
            CDSs (with protein)               :: 5,705
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 55 of 119
            Pseudo Genes (incomplete)         :: 64 of 119
            Pseudo Genes (internal stop)      :: 22 of 119
            Pseudo Genes (multiple problems)  :: 21 of 119
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-8050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC714_6"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            1..321
                     /locus_tag="JJ672_29745"
     CDS             1..321
                     /locus_tag="JJ672_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UMD26794.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTRAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGPDD
                     DC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            260..1018
                     /locus_tag="JJ672_29750"
                     /pseudo=""
     CDS             260..1018
                     /locus_tag="JJ672_29750"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            999..1496
                     /locus_tag="JJ672_29755"
     CDS             999..1496
                     /locus_tag="JJ672_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UMD26795.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            1503..1718
                     /locus_tag="JJ672_29760"
     CDS             1503..1718
                     /locus_tag="JJ672_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UMD26796.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            2112..2294
                     /locus_tag="JJ672_29765"
     CDS             2112..2294
                     /locus_tag="JJ672_29765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMD26793.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          3232..3405
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2744..3265)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          312..1834
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgctcacga tgtgggtgac ggaagacgaa caccggcggt tgcttgagcg gtgcgacgga
       61 aaacaactgg cggcgtggat gcgccagacc tgccttgatg agaaaccgac acgcgccggc
      121 aggcttccct cgatttcgcc cgcgctgctg cgtcagcttg ccggcatggg caataacctg
      181 aaccagatcg cccggaaagt gaacgccggt ggtgccggtc atgaccgggt gcagatcgtg
      241 gcagcgctga tggcgatcga tgccggtctg gaacggttgc ggcatgccgt actggaaaaa
      301 gggccggacg atgattgtta aattccatcc ccggggtcgc ggcgggggcg ccggtcctgt
      361 ggattatctt ctgggtaaag accggcagcg tgaaggtgcc agcgtcctgc aggggaagcc
      421 ggaagaagtc cgggagctga ttgatgcgtc gccgtatgtg aaaaaatata cctccggggt
      481 gctgtcattc gcggaggcgg acctgccgcc gggtcagcgt gaaaaactga tggccagctt
      541 tgagcgggtt ctgatgcccg gactcgataa agaccagtac agcattctgt gggtggaaca
      601 cgcagacaag ggtcgtcttg agctgaattt tctgatcccc aataccgaat tactgacggg
      661 gaaacgcctc cagccgtatt acgatcgtgc cgaccggccg cgcatcgatg cgtggcagac
      721 agtggtcaac ggcaggctgg ggctacatga tccgaacgac ccggagaacc ggcgggcgct
      781 ggtcacgcct tcaggactgc cagaaacgaa gcaggaagcc gcagagacga ttacacgggg
      841 tttgctgatg ttagcctcat caggagagct gaaaacgcgt gatgacgtca caggggcgct
      901 cacagcggcc ggttttgagg tggtcagaac cacaaaaaac agtatcagca ttgccgaccc
      961 ggacgggggg cgaaatatcc gactgagggg agccatttat gagcagtctt ttaacggcct
     1021 cgagggactt cgaggagaaa tcgaaagcac agcagcagct taccgaagag aggctgaaag
     1081 ccgcattcag caagcacgag aacgttgtca aatcggaact gaacgcaagc gcgaagagaa
     1141 tcaacgacgc catcagcgcc cacgaacagg ggatgaacgc ggcgatgcag tcgaaccggc
     1201 tgagcgtgct gcgaatggtc gggcggacgt ggctgaccat caccatggtg tcagggctgc
     1261 tgttcgccag cctgagcggg gtgctgtggt accaggggaa ccggatagcg gcgaacctgg
     1321 cggaaatcag gcagcagaga gatacgctgt cgaaactgca catgcagacg tggggcgtga
     1381 cctatctgga gaaccgcaac ggacggtttc tggtgctgcc ggaggggatg aaagccgaaa
     1441 cgggccggac ggtggacaac aagacgcgga acgcggtgaa gctggtacgg gagtagagca
     1501 gaatgacgga actggaaaaa caattgctga gcgcgttaga gcagctacag caggactact
     1561 cgagaaggct ggacgagtgg gagagcgcct tcgaggaatg gcggaagatg tctggtctta
     1621 tgcaacggga gaacgcggcg ctgagcgagc gcgtgtcggg cttgagcagg caggtgcaga
     1681 gtttgagcga gcagctgcgc cggttatcga caggctgaat gagattgagc aacagcgata
     1741 cgggcaaaca acggaaaagg aaccagagaa gacagcagat ctggtaccgg aacggcaacg
     1801 cacattatct catggtgaca gggagtatgg agactgataa acgcccgtct caggtggcct
     1861 gagcgtgacg gggatcatgg ccattttctg cgatttcagc acacagagag tcttttttaa
     1921 ccgaaatcac cggctatgca gtgatgtgaa taagtgatgc atatgcgtaa aataaatcag
     1981 gtgcgtttct ggtggttttc ggtgtacttg ccaccacctt ttgcccgtca gccatcagaa
     2041 acgccctgag tgcctcggcg cggtgcgggt aagagatgtt atgtttaatg ttgataacag
     2101 gagtaagagt gatgcttaca tttattggtg tagtggtagt cgccgttatt gcatttaaat
     2161 tatttatgct tttttttagt tcatccgaat ctgccgtcga acgtgctacc cgtaaatata
     2221 tggagaaccc tacgagtgct aactataaaa taatggtggc tacacgcatg agaaatgaga
     2281 agaaaagagg gtgatatatg tttgagttta ttggtttttt atttttactt ttggtgtgtt
     2341 atgtctttat acaaatggtt gctgtcaggg tgtttcctga atatggagta cggaaagcgg
     2401 aggaacgata ccgtcgaaat ccatcagcag aaaatgagga tctgttatgg agagctagaa
     2461 gtcgtataac taaacgcaag taatgtttcc taagtgtgtt gataggcaaa catggtggga
     2521 gttactactc tcggagttaa tgatcttctt gtgatcctgt cgtttcagtc gtaatctctt
     2581 gctctgaaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa gtcagttggg
     2641 gaactgctta accaggtaac tgggtatacg agaccgcaga taccaaaact gttctttcag
     2701 tgtagccgca gtcaggccca tacttcaaga cctctgaatc tctctctcgt atatcccagt
     2761 taccagtggc tgctgccagt ggcgctttgt cgtgctttct cgggttggac tcaagatgat
     2821 agttaccggg taaggcgcag cagtcggact gaacgggggg tttgtgcata cagtccagct
     2881 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat
     2941 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
     3001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     3061 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg
     3121 gccctatcgc ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt
     3181 cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
     3241 aagcggaata tatcctgtat cacatattct gctgacgcgc cggtgccgcc ttttttctcc
     3301 tgccacatga agcacttcac tgacatcctc atccgtgcca acatagtaag ccagtataca
     3361 ctccgctagc gctacgtgac tggttcaggg ctgcgccccg aaaccccact gacgcgcctg
     3421 cggcttgtcc aaccccgcgc cgaccgggaa aggctttccc gtccgtcacg gcgttatcag
     3481 gaggcgattc tggcagataa acgcaacaag
//
