LOCUS       CP067505                5366 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-8050 plasmid pC714_5, complete
            sequence.
ACCESSION   CP067505
VERSION     CP067505.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN17131928
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C714
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 162x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:15:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,953
            CDSs (total)                      :: 5,824
            Genes (coding)                    :: 5,705
            CDSs (with protein)               :: 5,705
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 55 of 119
            Pseudo Genes (incomplete)         :: 64 of 119
            Pseudo Genes (internal stop)      :: 22 of 119
            Pseudo Genes (multiple problems)  :: 21 of 119
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-8050"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC714_5"
                     /geo_loc_name="USA: South"
                     /collection_date="2017"
                     /collected_by="MDRO network"
     gene            1..192
                     /locus_tag="JJ672_29720"
     CDS             1..192
                     /locus_tag="JJ672_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMD26790.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLNALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1697..1906)
                     /locus_tag="JJ672_29725"
     CDS             complement(1697..1906)
                     /locus_tag="JJ672_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="UMD26791.1"
                     /translation="MKSIKNFVVVAALSMMSFGAFAQSISATASTLDRAEAKIAAQAAE
                     QGVSYKITSAQFNNRVHMTAELTK"
                     /name="DUF1471 domain-containing protein"
     gene            2165..3121
                     /locus_tag="JJ672_29730"
     CDS             2165..3121
                     /locus_tag="JJ672_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMD26792.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYIFYLKKEYNGEKIY
                     QIRKNKAGSNEFENNISKLLRSGVLSRVEDNDNYHVYPHLIGGFYEVFFVKGSDGAPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRPEQWKSKFYLDFQKENPDIDEA
                     KQFMPRFPRSSEINGMKIIVTQETNYIEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDRLGRHIDKARVGFILDRIIGIDNKKISEWKQESSKY
                     RGSSKVLSSKSPFSPYFDDEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            3105..3971
                     /locus_tag="JJ672_29735"
     CDS             3105..3971
                     /locus_tag="JJ672_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="UMD26788.1"
                     /translation="MEKEINTWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLDSFFNTFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNTSAMKRLESNQSPSVISIDFSFNEKTYDFDFLSLDG
                     DDNNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILD
                     SLLRKSEGKQLDGLLNVKGIRDVEIIERSSQRYRDLSSTVKSLPPFEDAYEKIACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            3968..4621
                     /locus_tag="JJ672_29740"
     CDS             3968..4621
                     /locus_tag="JJ672_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="UMD26789.1"
                     /translation="MIAFSFSDGGNRCENLDLFSTINCQNDMFGMVIMDGYSCSEGSME
                     FLSDRLGECVFSGDLDKNIIEPCKSVSVKAAVCFLFKDETHLHIYNAGDCRVYNKSGAL
                     LTEDNSVAWRTLKEKGVDNKKIPHLVCYCPDRNYLTSFVDFCSDEITFNHSVFDLNDEN
                     KEILLCTDGFWEHFDEGTVVDLMKDNPESNLAKFTKNIPDNAENHTVCYVRFPS"
                     /name="protein phosphatase 2C family protein"
     Typing          complement(4844..5217)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.756"
                     /coverage="91.0"
                     /evalue="2.13e-170"
ORIGIN
        1 atgacgaaac aacagaaaac agcgctgaat atggcaaaat tcatccagaa tcagtctcta
       61 ctcctgctgg agaagcttaa cgagcttgat ctcgatgttg aggccgacct ttgtgagaaa
      121 ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgaa tgcattacag
      181 gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta
      241 gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgaacgta
      301 atgcaaaaat ggtagatacc tcagagcttt tacgtgtctt tggtcccttt gagcaaccga
      361 agggtgaaca ccattctgga caggtctctg aacagcgtga taggggtcag tttggagatc
      421 ggaaattttt gtacgttaag cgcattattt aaccaatctt ggttcgtaag gggttgattt
      481 tgcgtcagtg gctatcgctt ttaagttgtc gctcttgcag tatcgcattt aggttatccg
      541 attaaggtca aacctctgaa aatgccgtat agcgcgggag tacaccctat tgctataggt
      601 aagtctgttc aaaaaacagg cttaccgtac aataattctc tatatccaaa ctgacccctg
      661 atacagcctc tgctgaccag agtgaacagg tgattcgtca tctgaaacag gagattgaac
      721 acctgaacac cttacttttg tccaaggatt cgcacattga cagcctgaaa caggccatgc
      781 tccttcttga aaatcaccag gcaaaattaa ctgaatcccc ggcgccatgg tggaaattct
      841 ggaaaaaatc ctgaccccgt catgcttcag ttttcctctc cggccatccc ggggagttcc
      901 tgaaccttcc caaaaatcgt cagttctctg aacgcacctc accacttaaa atacccacac
      961 accacgcaaa attaaaattt tgcagataac tcattaatat cagttagttg tgggttatta
     1021 aattacgcag tattactttc tgtaaggtga atccaagtcg tcggcgattc gtgtacgact
     1081 tgccgacgac tcgtcataga gtcatcgacg actcacttaa tactatcagt aacttatgac
     1141 gacttgactc tagagtcgaa tcgtcacttt ggtgtttttt tgaccttgac gactctagag
     1201 tcaagtcacg agtcgaatcg ccggtgaatc gtgtggtgag tcgtccggcg acgtgccata
     1261 acgtgattgc cggccgtcga cgaacccgat tcctgatcgt gctgaaaacc ggttttccgg
     1321 ctgcgccggc aacgcttcac ggccacgcaa taaattgcgt ccccgttcac cgttttcgga
     1381 tgggaagaac ggcagaaaca ggacttacca tggctgaatg aggtgaattt ccgcgctatg
     1441 cacttgcgcg catactcatg catgccgtga aaacggcctc agggcgtttc tggcgggttt
     1501 tcgggtggtt tgttgcctgt tttaccggtt tcccgtcgga aacgccctga ggccgtttta
     1561 gcggtgcgta caatcatgcc tttatgttaa atgacaggcg taaaaaagcc cccgaagggg
     1621 cttaagggtt ttcctgttga gggacgaacc gggccacctt ccggcgacct gttcggcagc
     1681 tccctctgcg accatcttat ttggtcagtt cagccgtcat gtggacacga ttattgaact
     1741 gagcgctagt gattttgtat gacacaccct gttctgcggc ctgtgcggca atttttgctt
     1801 cagcacgatc cagggttgaa gcggtagcgc tgatgctctg agcaaaggca ccgaaagaca
     1861 tcatagagag agcagcaact acaacgaaat ttttgatgga tttcatggtc gtattcctta
     1921 agtcattttg ttctttatgg cgctggatgt gaggagtaat gaaaacagcc acataagtaa
     1981 ttattcttga ctttctacat tagtcctata aaataaatcc ttaggattta ttttatagga
     2041 caatacaatc taacgcatgt caactcgttg tttagcattg atttttactt gacaaaccat
     2101 ctctaataga atatacgtcc ttaggacgta tattctatta gagactaaac cgaaaaggtt
     2161 gaaaatgtat cttattgaag aagcgctaac gtcttggttg ttgaacgaat ataaaaaacc
     2221 agtcataacc aaagaagaaa tttctttgca aatttatatc ttttatttaa aaaaagaata
     2281 taacggggag aaaatatatc aaatcaggaa aaataaagca ggatctaatg agtttgaaaa
     2341 taacatatca aaattattaa ggtctggagt tttatcaaga gttgaagata acgataatta
     2401 tcatgtttac ccacatttaa ttggcggttt ctatgaggtt ttttttgtta aaggtagtga
     2461 tggtgctcct gaaaacgata tagtgtgttc tacgtatcca tatggatatt tatcctattt
     2521 atccgctatg gattggcata atatcacaga taaaataccc aagataatcc actttacgac
     2581 atgccgtccc gagcagtgga agagtaagtt ctatctagat tttcaaaaag aaaatccaga
     2641 tatagatgaa gcaaaacaat tcatgcctag atttccaagg agttctgaaa ttaatggaat
     2701 gaaaattatc gttacacaag aaactaatta cattgagcca atgaaggcgc gtaattctcc
     2761 tgttagggtg tcctctatag ggaagacttt tatggatatg actagaagac ctgattactg
     2821 tggtggtgag gaacatgtat tggagtcatt tattgagcac ggtaaaaaat atgcgcctct
     2881 gattataaaa gaagttgata ggcttggtcg tcatattgac aaggcaaggg ttgggtttat
     2941 tttggataga attattggaa ttgataacaa aaaaataagc gaatggaagc aagagtcgag
     3001 taaatacaga ggtagttcga aagttctatc ttcaaaaagt ccgttctcac cttactttga
     3061 tgatgaatgg tttatttcta ttaatgttga tatggttaaa tcttatggaa aaagaaatta
     3121 atacttggat cgaagagtct ggcgggaagt acaggttgat gcggcaggct gtgcatataa
     3181 tactgaaatc tatttctata gaggagagtt tgaatgaaat gatggttatg aagggaggaa
     3241 cccttcttgg tgttaagcat ggtagtgata gatacacaaa agatattgat ttctctaccg
     3301 aaaaaaaact ttccgaaatc gatctagata gttttttcaa tacatttaat gactcaatgg
     3361 atgttgctga ggctgaattg ggatatggta ttaaatgtaa ggtacaatct cataaaatcc
     3421 agcctaatgc gcaaggaaca tttccaacgg tacagataaa tatagccttt tgcgatcgat
     3481 ctaatacgtc agcaatgaaa aggcttgaat ctaatcaatc accttcagtg ataagtatcg
     3541 attttagttt taacgagaaa acgtatgatt ttgatttttt gtcattagat ggcgacgata
     3601 acaacgatag tgtaaaaacc tacagcttaa ccgatttgat ggctgagaaa tatcgttctg
     3661 ttttacagca aaaagttaga gagcgaaatc gtgagcaaga tatatatgat attaattatc
     3721 ttttaggtaa gtatgaattt tctcggcaag ataagtataa aatactggat tcactcttaa
     3781 gaaagagtga aggtaaacaa ttagatgggc tcttaaatgt taaaggtata agggatgtgg
     3841 aaattattga acgttccagt caacgatatc gagatttatc atccaccgtt aaatcattgc
     3901 ctccttttga agatgcttat gaaaaaatcg cctgtttcta tgaaagtctt ccatgggata
     3961 tatttaaatg atagcattta gttttagtga tggtggtaat cgatgtgaaa acttagattt
     4021 attttctaca ataaattgtc aaaatgatat gtttgggatg gttatcatgg atggatattc
     4081 ctgctctgaa ggtagtatgg aattcctatc tgacaggcta ggtgagtgcg tgttttctgg
     4141 tgatcttgat aaaaatatta ttgaaccctg caagtcagtt tctgttaagg ctgcggtatg
     4201 tttccttttt aaagatgaga cgcacctcca tatctataat gcaggggatt gccgtgtata
     4261 taataaaagt ggggctcttc tgaccgagga taactcagtt gcttggagaa ccttaaagga
     4321 aaagggagtg gataataaaa aaatacctca cctcgtttgt tattgcccag acaggaatta
     4381 tctaacatca tttgtcgatt tctgttctga tgagataaca ttcaatcatt cagtttttga
     4441 tttaaatgat gaaaacaaag agattctttt atgtactgat ggcttttggg agcattttga
     4501 tgaaggtact gttgtcgatc ttatgaaaga taacccagaa tctaacttag ctaaattcac
     4561 caaaaacatc ccagataatg cagagaatca tacggtttgc tacgttagat ttccttcttg
     4621 atttcgtgtt ttttgaagaa gttattgaga tcttcgtgtc acatagataa tctctagtct
     4681 gtaaaagaaa aaaaccgcct ttgcggggcg gttttctctt aggttcaacg agtttgcggc
     4741 tcgaagaacc aaggtaactg gcttggagga gcacagtcac caaaacttgt tctttcagtt
     4801 tagccttaac aggcgcatga cttcaagact aactcctcta aatcagtaac cagtggctgc
     4861 tgccagtggc gcttttgcgt gtcttccggg ttggactcaa gacgatagtt accggataag
     4921 gcgcagcagt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     4981 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     5041 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag ggggaaacgc
     5101 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     5161 atgcttgtca ggggggcgga gcctatggaa aaacggccgt caggcggcct ttccttccat
     5221 catgctattg ctcaattttc tcgagcgttt ctacgtatgc tcctaccgct cgccgcagcc
     5281 gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg aaaagagaac atccctacac
     5341 tgagcactaa cgctctgagg cttctt
//
