LOCUS       CP067478                3991 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3304 plasmid pC763_5, complete
            sequence.
ACCESSION   CP067478
VERSION     CP067478.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868676
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C763
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:35:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,723
            CDSs (total)                      :: 5,598
            Genes (coding)                    :: 5,500
            CDSs (with protein)               :: 5,500
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 45 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 13 of 98
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3304"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC763_5"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            join(3824..3991,1..807)
                     /locus_tag="JJ153_28600"
     CDS             join(3824..3991,1..807)
                     /locus_tag="JJ153_28600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMC94624.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            1554..1745
                     /locus_tag="JJ153_28605"
     CDS             1554..1745
                     /locus_tag="JJ153_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMC94625.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1745..2167
                     /locus_tag="JJ153_28610"
     CDS             1745..2167
                     /locus_tag="JJ153_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UMC94626.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2971..3243
                     /locus_tag="JJ153_28615"
     CDS             2971..3243
                     /locus_tag="JJ153_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMC94623.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     Typing          complement(1035..1506)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.885"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 tgtgttcctt tcatatcatc atttcttcat gttgattact caagtataat atttcctgtg
       61 gctgatggtg gtggaactaa gttcatagaa caaatattgc ttcttatttc tgtttcgggg
      121 atctctgcgt atcttgggta cgcattactg gatggtcttg ccaacaaagt attaaaagag
      181 caagtagacg gtatagataa aaagcaacaa gatttggagg ctgaacaaga tgagtttaaa
      241 gatgagcttg ataggagcaa ggagctgata gaacagcttg aaatggataa gaaaacaaca
      301 aaattcgagc taggatactt taaagccatt tcagctgtgg ataaagcaga atcaatgatg
      361 tccattccgg atgaggctct ttctgttaag aaaaaactaa ctgaggcatt agatgctgta
      421 acggaatcat tgtctcttgt taaaagagag gatgtagcta aggatgacta cgacaagctc
      481 ttagtgctta aagcttatat tttgaaaaga cttgatcgta ttgatgatgc tctgtcaatt
      541 acggatgagt tacttatgag taatgaggat aaccctatac ttatttacaa taaggcttgc
      601 tatcaatata ttttgcgtcg ttgccaagct gataactccg atattaagga tatgattcgc
      661 agggctctga ctattaaagt tacagatcct gaattcattc gcaggcaaga aaaaataaga
      721 accaaggtca taggaaataa agataatgat cttgaagggt tgtttactga cgctgagctg
      781 gaggaattaa aggtggctat caaataggca gtggctagct ctatgaattc caaaaagatc
      841 cttgacgatc ctttgaggtg cagcgtactc tacccctcaa acgaaaaaac cacccggcaa
      901 ggtggttttt cgaaggttca gtaaccggca agtttctgaa ccgaggtaac tggcttagag
      961 gagcgcagtc accaaaacag ttcgtttagt ttagccttaa ctggcgcata acttcaagac
     1021 taactcctct aaatcaatta ccagtggctg ctgccaatgg cgataagtcg tgtctttccg
     1081 ggttggactc aagagatagt tactggaacg ggcgcagcag tcggactgaa cggggggttc
     1141 gtgcatacag tccagcttgg agcgaactgc cttcccggag ccgagtatca ggcatggaat
     1201 gagaaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     1261 gcgcacgagg gagccaccag ggggaaacgc ctggtatctt taagtcctgt cgggtttcgc
     1321 caccactgat ttgagcgtcc gtttccgcga tgcttgtcag gggggcggag cctatggaaa
     1381 aacagccgtt cggcggcctt tgcttttctg cctcatatct tggttgccta cgtttttatg
     1441 tacacgctcc agccgctcgc cgcagccgaa cggctggagc gtagcgactg agtgagcgag
     1501 gaagcggaaa agagactagt ttgacattga gcactgacgc tctgaggctt cttatgacaa
     1561 aacaacagaa aacagcgctg aacatggcta aattcatcca gaatcagtct ctgctcctgc
     1621 tggataagct taacgagctt gatctcgatg tcgaggccga cctttgtgag aaactccatg
     1681 atgatgcgga gcatcttttc cgtacactgt cctcgagact ggatgaatta caggatggta
     1741 actgatggcc atagtgtcta ttagtgaagc ggcacggctt gttaaggtta gtcgccccac
     1801 aatttacaag atgattaaca gtgggaagtt aagttacacc tctgtggtaa agcacgggaa
     1861 agctattaaa gttattgata cttcagagct aattagagtg tttggttctc ttgatggtgt
     1921 aatcgatact gtaaaatatg atgtaaaaag tgacgcagaa tctacaggta ttaacagcgt
     1981 tggtttacat gatttacaac atagaatcgc tttacttgaa gctgaaaacg atggtcttaa
     2041 aggggctgtt aaggctcgag atgagcatat tgattccctt cgtcaggcta tgcagcttct
     2101 ggagcacaag cacgaacctt cttcaccacc ccattccccg tggtggaaat tctggaaaaa
     2161 atcctgatcc cgccatgctt ccattttgct ctccggccat tccggggagt tcctgaacct
     2221 tcccaaaaat cgtcagatcc ctgaacgctc ctcaccacta aaaataccca cacactacgc
     2281 aaaattaaaa ttttgcagat agacctttat tttcaatcag ttatctattt taaattacgc
     2341 aattttattt tttgtaaggt gaatccgagt cgtggacgac tctatggcga atcgccgatg
     2401 acttgcccac gattcatatg tgaatcacgc catataatca gtacgttatg atgacttgac
     2461 tctagagtcg acttgccggg ttggtggctt tttgaccatg gcgactctag agtcaagtca
     2521 cgagtcgaat cgccggtgaa tcgtgtggtg agtcgtccgg cgacttgcca taatgtgatt
     2581 gctggtctcg acaaacccga ttcctgaacg tgctgaaaac cggttttccg gctgcgccgg
     2641 caacgcttca cggccacgca ataaattgcg cacccgttca ccgttttcag tctggtttgg
     2701 tgcgggtttt gagttgcctg gcctgaattc aggctgaaat ccccagacag tcagtcaagt
     2761 gcataacgat tcatgcccgt aaaacgccct ggagcgattt tgaggcgttt tcagggggta
     2821 agacgtagga tttaatgggt tacgccccag aatcgttctg aggccgtttt agcggtgcgt
     2881 gtaatgggac gttatggtaa atcaacggcg gatgcctgag tctgtgttag agtcagcgga
     2941 ttgtgataat cagtgactca gggagatgtt ttggctactg acgctcaggt aaaagaaatt
     3001 aatgcactca tcaaaaaata cccggactca tgctccatct gtcatgaagt ctatgatgag
     3061 gatgatgtga cctacaccgt ttttgggtat gaccggaaag gtaaaataca ggtcaccacg
     3121 ggatgctgtg ccggaatgct gacagagccg gttctgctgg gtgtctgtgg gtgctttgac
     3181 ccggaagaac gtgacgagat catgcagaat caccccatgg caaagcagtt tttcacagag
     3241 tgattccgta tctgttgagt ctgaagcccg ccttgtgcgg gctttttcat atctgcgtga
     3301 aatgaggcgt tatggtaaat gcacgcattc attcttttga aaaatccaga atgacgaata
     3361 catggggcat gtttatatga ctattgagag gaaggatgtt attgtaaaaa aatcctttaa
     3421 attcaatcat ttaattatgg tagccaaagg tgcggggctt cgcttattcc taaggaataa
     3481 gcgaagcccc gcacctttgg ctaccagtgc attatacttt ttttttcacc caatgtcaat
     3541 acatgggttg attttatcat tttaagcatt tttgttgttg tgagtgctgg gtttatgccc
     3601 tatattcagc gctaagcaag catgggcttg caaaggaaca attaatgaga ggtgctctat
     3661 gaaaaaaatc aaacttcacg atgagcgtcc agacttgtaa gtgtcaagtc agcagaaaaa
     3721 ggcagccaag gctgcctttt ttgttattgg ttagtatatg attgagctta tcttaactta
     3781 cctgaacaaa gttttattgt ttgctttgcg caaggattct ctcatggctt tttttaacct
     3841 attgtttgtt gcatctctaa tttgttttgg tggtgttatg gggagctatt ccagaggttg
     3901 ccgagactcc caaaataaat tttcaaaaga taaggatgag aataataaaa gagctagtgt
     3961 ttatagattt ggtatagcat ccgcatttat t
//
