LOCUS       CP067477                6352 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-3304 plasmid pC763_4, complete
            sequence.
ACCESSION   CP067477
VERSION     CP067477.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15868676
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C763
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 15:35:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,723
            CDSs (total)                      :: 5,598
            Genes (coding)                    :: 5,500
            CDSs (with protein)               :: 5,500
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 44 of 98
            Pseudo Genes (incomplete)         :: 45 of 98
            Pseudo Genes (internal stop)      :: 23 of 98
            Pseudo Genes (multiple problems)  :: 13 of 98
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3304"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC763_4"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            complement(join(6279..6352,1..580))
                     /locus_tag="JJ153_28560"
     CDS             complement(join(6279..6352,1..580))
                     /locus_tag="JJ153_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="UMC94620.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="protein phosphatase 2C family protein"
     gene            complement(577..1443)
                     /locus_tag="JJ153_28565"
     CDS             complement(577..1443)
                     /locus_tag="JJ153_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="UMC94621.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            complement(1427..2383)
                     /locus_tag="JJ153_28570"
     CDS             complement(1427..2383)
                     /locus_tag="JJ153_28570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMC94622.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            complement(3248..3481)
                     /locus_tag="JJ153_28575"
     CDS             complement(3248..3481)
                     /locus_tag="JJ153_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UMC94616.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3488..4018)
                     /locus_tag="JJ153_28580"
     CDS             complement(3488..4018)
                     /locus_tag="JJ153_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UMC94617.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTAALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(3996..4706)
                     /locus_tag="JJ153_28585"
                     /pseudo=""
     CDS             complement(3996..4706)
                     /locus_tag="JJ153_28585"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4696..5019)
                     /locus_tag="JJ153_28590"
     CDS             complement(4696..5019)
                     /locus_tag="JJ153_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UMC94618.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLAEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELCSLRQVVREQGAR
                     NDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5084..5275
                     /locus_tag="JJ153_28595"
     CDS             5084..5275
                     /locus_tag="JJ153_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMC94619.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(5361..5443)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="3.99e-36"
     Typing          5459..6016
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.831"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3145..4706)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.585"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4703..5052)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.744"
                     /coverage="99.0"
                     /evalue="6.74e-62"
ORIGIN
        1 agttagattc tgggttatct ttcatgaggt caataacagt accatcatta aaatgctccc
       61 aaaacccatc agtacataaa ataacctctt tgttttcatc atttaaatca aaaactgaat
      121 gattgaaaat tatctcatca gagcagaaat caacaaatga tgttagataa tttctgtctg
      181 ggcaataaca aacgaggtga ggtatttttt tattatcgat tccattttcc tttaatcttt
      241 tccaggcaac cgagttatcc tctgttagaa ggactccact tttgttatat acacggcaat
      301 ctcctgcgtt atatatatgg aggtgcgtat tatttttaaa aaggaaacat actgcagcct
      361 taacaggaac tgacttgcat ggttcaataa tatctttatt cagatcacca gaaaacacgc
      421 acttatctaa cctaccagag agaattttca tattccactc agagcaggaa tacccatcca
      481 taataaccat tccaaacaca tcattttgac aatttatcgt agataataag tctaagtttt
      541 cacatctgtt accaatatca ctaaaactga acgctatcat ttgaatatat cccacggaag
      601 actttcatag aaacaggcaa ctttttcata agcatcttca aaaagaggta atgatttaac
      661 ggtggatgat aaatcctgat atcgctgact ggaacgctcg ataatttcta catcccttat
      721 acctttaacg tttaagagat tatccaattg tttaccttca ctctttttta aaagagactc
      781 cagtatttta tacttatctt gccgagaaaa ctcatattta cctaaaagat aattgatatc
      841 atatatatct tgctctcgat ttctctctct aactttttgt tgcaaaacgg atcgatattt
      901 ttcagccatc aaatcagtta agctgtaggt ctttacgcta tcattgtcat catcaccatc
      961 taatgataaa aagtcaaaat catacgtttt ttcgttaaag ctaaaatcga tatttatcac
     1021 tgaaggtgat tgattagatt caagcctttt cattgcagac gcattagaac gatcgcaaaa
     1081 ggctatattt atctgtacag ttggaaatgt tccttgtgca ttaggttgaa ttttatgaga
     1141 ttgaacttta catttaatac catatcccaa ttcagcctca gcaacatcca ttgagtcatt
     1201 aaacctattg aaaaaatccc ccaagtcaat ttcagaaagt tttttttcgg tagaaaaatc
     1261 aatatccttt gtgtatctat cactaccatg cttaacacca agaagagtcc ctcccttcat
     1321 aaccatcatt tcattcaaac tctcctctat agaaatagat ttaagtatta tatgtacagc
     1381 ctggcgcatc aacctgtact tcccaccaga ctcttcgatc caattattaa tttctttttc
     1441 cataagactt aaccatatca acattaatag aaataaacca ttcttcatca aagtaaggtg
     1501 agaatggact ctttggagat agcactttcg aactacctct gtatttaccc gcctcttgtt
     1561 tccattcgct tattttttta ttattaaccc caataattct atccaaaatg aatccaaccc
     1621 ttaccttgtc aatatgacgg ccaagcttat cgacttcctt tataatcaga ggtgcatatt
     1681 ttttaccgtg ctcaataaat gattccaata catgctcctc accaccacag taatcaggcc
     1741 gcctggtcat atccataaag gtcttaccga tagatgatac cctgacaggg gaattacgag
     1801 ccttcattgg ctcaacataa tttgtttctt gtgtaacgat aatttttatt ccattgacct
     1861 cagaatttct tggaaatcta ggcatgaatt gtttggcttc gtctatatca ggattatcct
     1921 tttgaaaatc atgatagaac ttattcttcc actgttcgga acgacatgtc gtaaagtgga
     1981 ttatcttggg tattttatct gtgatgttat gccaatccat agcggataaa taggataagt
     2041 atccatatgg atacgtagag cacactatat cattttccgg agaaccatcg ttacctttaa
     2101 caaaaaagac ctcatagaaa ccgccaatta aatgggggta agcatgatgc ttatcgttac
     2161 tttcaactct tgataaaacc cctgacttta ataattttaa tatatttttt tcaaattcat
     2221 taggtcctgc tttgttcttt ctaatttgat atatcctctc accattatat tcttttttta
     2281 aataaaaaag gtaaatctgc aaagagattt cttctttggt tatgactggt tttttatatt
     2341 cattcaacaa ccaagacgtt agcgcttctt cgataagata catattcaac cttttcggtt
     2401 tagtctctaa tagaatatac gtcctaagga cgtatattct attagagatt gattgtcaag
     2461 aaaaaaataa taacaaacaa gcatttgcca tttttatatt tttgatatcc tataaaataa
     2521 atccttagga tttattttat aggataataa gtaggaagtc aagaaaaagt ttacttatat
     2581 caccgttttc ataacacctc atgcgatcaa ttcagcggcg gttatagttc aaatccatga
     2641 ttaggacatc tgttgctcat aaacgatgag cgtaaaagat catttttcag cgtcatttac
     2701 cataacgtcg cattgtacgc accgccaaaa cgcgttcggt gcgatcacgg cagcagacag
     2761 gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc gcacccgata aattttaacc
     2821 acatgcatag ctatgcagcc atgtgaatca cgcaggaatt gccagccaga gactgctgaa
     2881 acgtattttt ttcatacttt cggaaaggca gaagacggaa ccggagcgga aaaacagaaa
     2941 ttcaggggaa tgagcgcgat ctggcaataa cggcaaaaca cagcaacaaa gacacaccag
     3001 aatcgcgccc gtatgcgttt taacgcgttc ctgtgttttc agcaagctct gactgaagcg
     3061 cgttcccggt gcccccgaac caaccattat cccaaaaaat gcaccggaaa cgcgctgcca
     3121 acattttagc ctgtctctgg ttaccatccc agcgaagggc catccagtgt ccgttcctga
     3181 atttccggct ggcgctcccg ttccagagat aactcatgtt cgcgttcctg catcagccgt
     3241 tcatccctta tctcctgttc tcgctgtata actggctcaa gcgttctgtc cgctcgctca
     3301 agtgctgcac ctgctgactc aactgcatga cccgcccgtt cagcatcgcg ttgtcccgtt
     3361 gcgtaagcga aaacattttc tgcaattcca cgaaggcgct ctcccattcg ttcagccgct
     3421 gcatatagtc ctgttgcagt tgctctaatg cgttcagcaa atgtctttcc agctccgtca
     3481 tgctctgtca ctcctgcacc agtctcactg cgttcagttt gccgttgtcc ttcgtccagt
     3541 tcgtttcggc tttcatcccc ttcggcaaca ccagaaaacg tccgttgctg tcctcgtgat
     3601 aacgcacgcc ccatgtcttc gcattcagct tctccagact ctctttctgc tcgcggagaa
     3661 tattcatatt cgcgtttatc ttcatgccct ggtaccacag cagactcccg gtcgtggcaa
     3721 acatcagtac tgagacgata atcagccaca gccagcgttt cacgctcatt ttcagcacgg
     3781 tttgccagtg tgtgtccatc gtgctgacgg tcgttttctg atacctctcc agaagttctt
     3841 tcagggctgc tgtatggtcg ttgatagcgt tcctgattaa agattcgttt gaggtcagtt
     3901 ccctgtttac agattcttcg agcttcctga actcgtcgtt cagcattttc tctgtagatt
     3961 cggctctctc tttcagcttt ttctcgaact ccgcgcccgt ctgcaaaaga ttgctcataa
     4021 aatgctcctt tcagcctgat attccttccg ccgttcgggt ctgcaatgct gatactgctt
     4081 cgcgtcaccc tgacaacctc aactcccaca tctgtgagtg cctgaatcac atcctgacgg
     4141 tcttttatct ctccggcacg gtaaagcaca tcaagccccc gtgtaacgct ctcagcaatc
     4201 gcctgtttcg tttccggcag gttatcaggg agtgtcagcg tcctgcggtt ctccggggca
     4261 ttcgggtcat gcagcccgta atggtgattc atcagcgtct gccaggcatc aattctcggc
     4321 ctgtctgcgc ggtcatagta cggctggagg cgttttccgc tctgtagctc catgttcgga
     4381 atgacaaaat tcagctcaag ccgtcccttg tcctggtgct ccacccacag gatgctgtac
     4441 tgattttttt cgagaccggg catcagtaca cgctcaaagc tcgccatcac tttttcacgc
     4501 cctcccggcg gcagctcctt ctccgcgaac gacagaacac ctgacgtgta tttcttcgca
     4561 aatagcgtgg catcgatgag ttcccggact tcttccggat taccctgaag caccgttgcc
     4621 ccttcccggt tacgctccct tcccagcagg taatcaaccg gaccactgcc accacctttc
     4681 cccctggcat gaaatttaac aatcattccg cgctccctgt tccctgacga cctgccgtaa
     4741 gctgcacaac tctctctcga tggccatcag tgcggccacc acctgaaccc ggtcaccgga
     4801 agaccactgc ccgctgttca ccttccttgc tgtctgattc aggttattcc cgatggcagc
     4861 cagctgacgc agtaacggcg gcgccagtgt cggcagcttc ccggaacggg caaccggctc
     4921 agccaggcag acccgccgca tccataccgc cagttgttta ccctcacagc gttccagtaa
     4981 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctcgcgtt tcatcggtat
     5041 cattatcccc atgaacagta atccccctta cacggaggca tcagtgacta aacaggaaaa
     5101 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgctgc tggagaagct
     5161 caacgaactg gacgcggatg aacaggctga tatctgtgaa tcgcttcacg accacgccga
     5221 tgagctttac cgcagctgcc tcgcacgttt cggggatgac ggtgaaaacc tctgacacat
     5281 gcagctcccg gagacggtca cagcttgcct gtgagcggat gccgggagca gacaagcccg
     5341 tcagggcgcg tcagcgggtt ttagcgggtg tcggggcgca gccatgaccc agtcacgtag
     5401 cgatagcgga gtgtatactg gcttaactat gcggcatcag tgcgaattgt atgaaaagtg
     5461 ccccatgccg ggtgtgaaat tccgcacaga tgcgtaagga gaaaatgcgc gtccaggcgc
     5521 ttttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta
     5581 ctgacttaca caaaaacggc agcacggtta tccacagaat caggggataa ggccggaaag
     5641 aacatgtgag caaaagacca agaacaggaa gaaggccgcg ccgcaggcgt ttttccatag
     5701 gctccgcccc cctgacgagc atcacagaaa tcgacgctca agtcagaggt ggcgaaaccc
     5761 gacaggactt aaagatacca gacgtttccc cctggtggct ccctcgtgcg ctctcctgtt
     5821 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5881 tctcatagct cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5941 tgtgtgcacg aaccccccgt tcagcccgac tgctgcgcct tatccggtaa ctatcgtctt
     6001 gagtccaacc cggtaagaca cgccttaacg ccactggcag cagccactgg taactgattt
     6061 agaggagtta gtcttgaagt catgcgccag ttaaggctaa actgaaagaa caggttttgg
     6121 tgactgcgct cctccaagcc agttaccttg gttcttcgag ccgcaaactc tttgaaccta
     6181 agagaaaacc gccccgcaaa ggcggttttt ttcttttaca gaaaagagat tatctatatg
     6241 acacgaagat ctcaatagct tcttcaaaaa acacgaaatc aagaagggaa tctaacgtag
     6301 caaaccgtat gattctctgc gttacccggg atatttttag taaatttagc ca
//
