LOCUS       CP067452                6352 bp    DNA     circular BCT 01-MAR-2022
DEFINITION  Klebsiella pneumoniae strain ARLG-4929 plasmid pC957_4, complete
            sequence.
ACCESSION   CP067452
VERSION     CP067452.1
DBLINK      BioProject: PRJNA658369
            BioSample: SAMN15869162
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Shropshire,W.C.
  TITLE     Highly Conserved and Distinct Accessory Genomes Drive Independent
            and Parallel Endemic Spread of Carbapenem-Resistant Klebsiella
            pneumoniae Clonal Groups 258 and 307
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6352)
  AUTHORS   Shropshire,W.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2021) HMH-CDI, Hackensack-Meridian Health Center
            for Discovery and Innovation, 340 Kingsland Street, Building 102,
            Nutley, NJ 07110, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.7
            Assembly Name                     :: C957
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 191x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2021 16:26:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,746
            CDSs (total)                      :: 5,621
            Genes (coding)                    :: 5,514
            CDSs (with protein)               :: 5,514
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 44 of 107
            Pseudo Genes (incomplete)         :: 54 of 107
            Pseudo Genes (internal stop)      :: 23 of 107
            Pseudo Genes (multiple problems)  :: 13 of 107
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-4929"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC957_4"
                     /geo_loc_name="USA: Midwest"
                     /collection_date="2016"
                     /collected_by="MDRO network"
     gene            1..192
                     /locus_tag="JJ639_28675"
     CDS             1..192
                     /locus_tag="JJ639_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UMC59481.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1196..1849)
                     /locus_tag="JJ639_28680"
     CDS             complement(1196..1849)
                     /locus_tag="JJ639_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C family protein"
                     /protein_id="UMC59484.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="protein phosphatase 2C family protein"
     gene            complement(1846..2712)
                     /locus_tag="JJ639_28685"
     CDS             complement(1846..2712)
                     /locus_tag="JJ639_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="UMC59482.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            complement(2696..3652)
                     /locus_tag="JJ639_28690"
     CDS             complement(2696..3652)
                     /locus_tag="JJ639_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UMC59483.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            complement(4517..4750)
                     /locus_tag="JJ639_28695"
     CDS             complement(4517..4750)
                     /locus_tag="JJ639_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UMC59478.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4757..5287)
                     /locus_tag="JJ639_28700"
     CDS             complement(4757..5287)
                     /locus_tag="JJ639_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UMC59479.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTAALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(5265..5975)
                     /locus_tag="JJ639_28705"
                     /pseudo=""
     CDS             complement(5265..5975)
                     /locus_tag="JJ639_28705"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5965..6288)
                     /locus_tag="JJ639_28710"
     CDS             complement(5965..6288)
                     /locus_tag="JJ639_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UMC59480.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLAEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELCSLRQVVREQGAR
                     NDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(278..360)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="97.0"
                     /evalue="3.99e-36"
     Typing          376..933
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.831"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4414..5975)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.585"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5972..6321)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.744"
                     /coverage="99.0"
                     /evalue="6.74e-62"
ORIGIN
        1 gtgactaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
       61 acgctgctgg agaagctcaa cgaactggac gcggatgaac aggctgatat ctgtgaatcg
      121 cttcacgacc acgccgatga gctttaccgc agctgcctcg cacgtttcgg ggatgacggt
      181 gaaaacctct gacacatgca gctcccggag acggtcacag cttgcctgtg agcggatgcc
      241 gggagcagac aagcccgtca gggcgcgtca gcgggtttta gcgggtgtcg gggcgcagcc
      301 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagtgc
      361 gaattgtatg aaaagtgccc catgccgggt gtgaaattcc gcacagatgc gtaaggagaa
      421 aatgcgcgtc caggcgcttt tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
      481 ggctgcggcg agcggtactg acttacacaa aaacggcagc acggttatcc acagaatcag
      541 gggataaggc cggaaagaac atgtgagcaa aagaccaaga acaggaagaa ggccgcgccg
      601 caggcgtttt tccataggct ccgcccccct gacgagcatc acagaaatcg acgctcaagt
      661 cagaggtggc gaaacccgac aggacttaaa gataccagac gtttccccct ggtggctccc
      721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      781 cgggaagcgt ggcgctttct catagctcac gctgttggta tctcagttcg gtgtaggtcg
      841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgactgc tgcgccttat
      901 ccggtaacta tcgtcttgag tccaacccgg taagacacgc cttaacgcca ctggcagcag
      961 ccactggtaa ctgatttaga ggagttagtc ttgaagtcat gcgccagtta aggctaaact
     1021 gaaagaacag gttttggtga ctgcgctcct ccaagccagt taccttggtt cttcgagccg
     1081 caaactcttt gaacctaaga gaaaaccgcc ccgcaaaggc ggtttttttc ttttacagaa
     1141 aagagattat ctatatgaca cgaagatctc aatagcttct tcaaaaaaca cgaaatcaag
     1201 aagggaatct aacgtagcaa accgtatgat tctctgcgtt acccgggata tttttagtaa
     1261 atttagccaa gttagattct gggttatctt tcatgaggtc aataacagta ccatcattaa
     1321 aatgctccca aaacccatca gtacataaaa taacctcttt gttttcatca tttaaatcaa
     1381 aaactgaatg attgaaaatt atctcatcag agcagaaatc aacaaatgat gttagataat
     1441 ttctgtctgg gcaataacaa acgaggtgag gtattttttt attatcgatt ccattttcct
     1501 ttaatctttt ccaggcaacc gagttatcct ctgttagaag gactccactt ttgttatata
     1561 cacggcaatc tcctgcgtta tatatatgga ggtgcgtatt atttttaaaa aggaaacata
     1621 ctgcagcctt aacaggaact gacttgcatg gttcaataat atctttattc agatcaccag
     1681 aaaacacgca cttatctaac ctaccagaga gaattttcat attccactca gagcaggaat
     1741 acccatccat aataaccatt ccaaacacat cattttgaca atttatcgta gataataagt
     1801 ctaagttttc acatctgtta ccaatatcac taaaactgaa cgctatcatt tgaatatatc
     1861 ccacggaaga ctttcataga aacaggcaac tttttcataa gcatcttcaa aaagaggtaa
     1921 tgatttaacg gtggatgata aatcctgata tcgctgactg gaacgctcga taatttctac
     1981 atcccttata cctttaacgt ttaagagatt atccaattgt ttaccttcac tcttttttaa
     2041 aagagactcc agtattttat acttatcttg ccgagaaaac tcatatttac ctaaaagata
     2101 attgatatca tatatatctt gctctcgatt tctctctcta actttttgtt gcaaaacgga
     2161 tcgatatttt tcagccatca aatcagttaa gctgtaggtc tttacgctat cattgtcatc
     2221 atcaccatct aatgataaaa agtcaaaatc atacgttttt tcgttaaagc taaaatcgat
     2281 atttatcact gaaggtgatt gattagattc aagccttttc attgcagacg cattagaacg
     2341 atcgcaaaag gctatattta tctgtacagt tggaaatgtt ccttgtgcat taggttgaat
     2401 tttatgagat tgaactttac atttaatacc atatcccaat tcagcctcag caacatccat
     2461 tgagtcatta aacctattga aaaaatcccc caagtcaatt tcagaaagtt ttttttcggt
     2521 agaaaaatca atatcctttg tgtatctatc actaccatgc ttaacaccaa gaagagtccc
     2581 tcccttcata accatcattt cattcaaact ctcctctata gaaatagatt taagtattat
     2641 atgtacagcc tggcgcatca acctgtactt cccaccagac tcttcgatcc aattattaat
     2701 ttctttttcc ataagactta accatatcaa cattaataga aataaaccat tcttcatcaa
     2761 agtaaggtga gaatggactc tttggagata gcactttcga actacctctg tatttacccg
     2821 cctcttgttt ccattcgctt atttttttat tattaacccc aataattcta tccaaaatga
     2881 atccaaccct taccttgtca atatgacggc caagcttatc gacttccttt ataatcagag
     2941 gtgcatattt tttaccgtgc tcaataaatg attccaatac atgctcctca ccaccacagt
     3001 aatcaggccg cctggtcata tccataaagg tcttaccgat agatgatacc ctgacagggg
     3061 aattacgagc cttcattggc tcaacataat ttgtttcttg tgtaacgata atttttattc
     3121 cattgacctc agaatttctt ggaaatctag gcatgaattg tttggcttcg tctatatcag
     3181 gattatcctt ttgaaaatca tgatagaact tattcttcca ctgttcggaa cgacatgtcg
     3241 taaagtggat tatcttgggt attttatctg tgatgttatg ccaatccata gcggataaat
     3301 aggataagta tccatatgga tacgtagagc acactatatc attttccgga gaaccatcgt
     3361 tacctttaac aaaaaagacc tcatagaaac cgccaattaa atgggggtaa gcatgatgct
     3421 tatcgttact ttcaactctt gataaaaccc ctgactttaa taattttaat atattttttt
     3481 caaattcatt aggtcctgct ttgttctttc taatttgata tatcctctca ccattatatt
     3541 ctttttttaa ataaaaaagg taaatctgca aagagatttc ttctttggtt atgactggtt
     3601 ttttatattc attcaacaac caagacgtta gcgcttcttc gataagatac atattcaacc
     3661 ttttcggttt agtctctaat agaatatacg tcctaaggac gtatattcta ttagagattg
     3721 attgtcaaga aaaaaataat aacaaacaag catttgccat ttttatattt ttgatatcct
     3781 ataaaataaa tccttaggat ttattttata ggataataag taggaagtca agaaaaagtt
     3841 tacttatatc accgttttca taacacctca tgcgatcaat tcagcggcgg ttatagttca
     3901 aatccatgat taggacatct gttgctcata aacggtgagc gtaaaagatc atttttcagc
     3961 gtcatttacc ataacgtcgc attgtacgca ccgccaaaac gcgttcggtg cgatcacggc
     4021 agcagacagg taaaaatggc aacaaaccac ccgaaaaact gccgcgatcg cacccgataa
     4081 attttaacca catgcatagc tatgcagcca tgtgaatcac gcaggaattg ccagccagag
     4141 actgctgaaa cgtatttttt tcatactttc ggaaaggcag aagacggaac cggagcggaa
     4201 aaacagaaat tcaggggaat gagcgcgatc tggcaataac ggcaaaacac agcaacaaag
     4261 acacaccaga atcgcgcccg tatgcgtttt aacgcgttcc tgtgttttca gcaagctctg
     4321 actgaagcgc gttcccggtg cccccgaacc aaccattatc ccaaaaaatg caccggaaac
     4381 gcgctgccaa cattttagcc tgtctctggt taccatccca gcgaagggcc atccagtgtc
     4441 cgttcctgaa tttccggctg gcgctcccgt tccagagata actcatgttc gcgttcctgc
     4501 atcagccgtt catcccttat ctcctgttct cgctgtataa ctggctcaag cgttctgtcc
     4561 gctcgctcaa gtgctgcacc tgctgactca actgcatgac ccgcccgttc agcatcgcgt
     4621 tgtcccgttg cgtaagcgaa aacattttct gcaattccac gaaggcgctc tcccattcgt
     4681 tcagccgctg catatagtcc tgttgcagtt gctctaatgc gttcagcaaa tgtctttcca
     4741 gctccgtcat gctctgtcac tcctgcacca gtctcactgc gttcagtttg ccgttgtcct
     4801 tcgtccagtt cgtttcggct ttcatcccct tcggcaacac cagaaaacgt ccgttgctgt
     4861 cctcgtgata acgcacgccc catgtcttcg cattcagctt ctccagactc tctttctgct
     4921 cgcggagaat attcatattc gcgtttatct tcatgccctg gtaccacagc agactcccgg
     4981 tcgtggcaaa catcagtact gagacgataa tcagccacag ccagcgtttc acgctcattt
     5041 tcagcacggt ttgccagtgt gtgtccatcg tgctgacggt cgttttctga tacctctcca
     5101 gaagttcttt cagggctgct gtatggtcgt tgatagcgtt cctgattaaa gattcgtttg
     5161 aggtcagttc cctgtttaca gattcttcga gcttcctgaa ctcgtcgttc agcattttct
     5221 ctgtagattc ggctctctct ttcagctttt tctcgaactc cgcgcccgtc tgcaaaagat
     5281 tgctcataaa atgctccttt cagcctgata ttccttccgc cgttcgggtc tgcaatgctg
     5341 atactgcttc gcgtcaccct gacaacctca actcccacat ctgtgagtgc ctgaatcaca
     5401 tcctgacggt cttttatctc tccggcacgg taaagcacat caagcccccg tgtaacgctc
     5461 tcagcaatcg cctgtttcgt ttccggcagg ttatcaggga gtgtcagcgt cctgcggttc
     5521 tccggggcat tcgggtcatg cagcccgtaa tggtgattca tcagcgtctg ccaggcatca
     5581 attctcggcc tgtctgcgcg gtcatagtac ggctggaggc gttttccgct ctgtagctcc
     5641 atgttcggaa tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc cacccacagg
     5701 atgctgtact gatttttttc gagaccgggc atcagtacac gctcaaagct cgccatcact
     5761 ttttcacgcc ctcccggcgg cagctccttc tccgcgaacg acagaacacc tgacgtgtat
     5821 ttcttcgcaa atagcgtggc atcgatgagt tcccggactt cttccggatt accctgaagc
     5881 accgttgccc cttcccggtt acgctccctt cccagcaggt aatcaaccgg accactgcca
     5941 ccacctttcc ccctggcatg aaatttaaca atcattccgc gctccctgtt ccctgacgac
     6001 ctgccgtaag ctgcacaact ctctctcgat ggccatcagt gcggccacca cctgaacccg
     6061 gtcaccggaa gaccactgcc cgctgttcac cttccttgct gtctgattca ggttattccc
     6121 gatggcagcc agctgacgca gtaacggcgg cgccagtgtc ggcagcttcc cggaacgggc
     6181 aaccggctca gccaggcaga cccgccgcat ccataccgcc agttgtttac cctcacagcg
     6241 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctcgcgttt
     6301 catcggtatc attatcccca tgaacagtaa tcccccttac acggaggcat ca
//
