LOCUS       CP066211                4466 bp    DNA     circular BCT 26-MAY-2021
DEFINITION  Enterococcus faecium strain 18-204 plasmid p18-204_6, complete
            sequence.
ACCESSION   CP066211
VERSION     CP066211.1
DBLINK      BioProject: PRJNA682333
            BioSample: SAMN16988098
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Moreaux,J., Zouari,A., Potrel,S.,
            Collet,A., Lecourt,M., Auger,G. and Cattoir,V.
  TITLE     Genetic features of the poxtA linezolid resistance gene in human
            enterococci from France
  JOURNAL   J Antimicrob Chemother (2021) In press
   PUBMED   33895846
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Dejoies,L., Sassi,M., Schutz,S., Zouari,A., Potrel,S., Collet,A. and
            Cattoir,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2020) Rennes1 University, U1230, 2 avenue du
            Professeur Leon Bernard, Rennes 35043, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2020
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/16/2020 11:29:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 5.0
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,926
            CDSs (total)                      :: 2,836
            Genes (coding)                    :: 2,412
            CDSs (with protein)               :: 2,412
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 424
            CDSs (without protein)            :: 424
            Pseudo Genes (ambiguous residues) :: 0 of 424
            Pseudo Genes (frameshifted)       :: 369 of 424
            Pseudo Genes (incomplete)         :: 48 of 424
            Pseudo Genes (internal stop)      :: 24 of 424
            Pseudo Genes (multiple problems)  :: 16 of 424
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="18-204"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p18-204_6"
                     /geo_loc_name="France: Creteil"
                     /lat_lon="48.7989 N 2.4658 W"
                     /collection_date="2018-04"
                     /collected_by="NRC for Enterococci"
     gene            complement(join(4197..4466,1..165))
                     /locus_tag="I8F51_14615"
     CDS             complement(join(4197..4466,1..165))
                     /locus_tag="I8F51_14615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QVX03368.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     gene            complement(571..744)
                     /locus_tag="I8F51_14620"
     CDS             complement(571..744)
                     /locus_tag="I8F51_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="QVX03369.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1429..1968)
                     /locus_tag="I8F51_14625"
     CDS             complement(1429..1968)
                     /locus_tag="I8F51_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="QVX03370.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(1984..2907)
                     /locus_tag="I8F51_14630"
     CDS             complement(1984..2907)
                     /locus_tag="I8F51_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="QVX03371.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     Typing          complement(1985..2907)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttatagcaa gtaaatccac gatgattatg attatatcgt gaacttgcag cttccacttt
       61 tatacctcta gcactaaaag gaaacagatt taacggagta cttgctccaa caatcatacc
      121 gaacaaaaat agacaagtaa taattttaat aattttattt ttcattgtta cttcctcctt
      181 agatataaaa tcctaggaaa gcgctttctg tatatattta gaataatcag aaaattaaat
      241 attgtcaaga gggtttattt tataaaacgt gttattttaa aggttagtag acataatgtc
      301 gactagacca agttgcccct ctaccaatgg atagagacgc tcactttgtg atttttaatt
      361 ggtctaggta aaaagctctt tttaaggctt gattttctgt agttgcttct ttccgtttct
      421 ttgtgaattt ttaccagtca ttttttactg gattgttatt ttttataaaa tatgataaaa
      481 ttttggtagc gctttataag cgcgttgctt tttcaagaat aaactctcat tattttttta
      541 actggctgct cgaaagagca gtctttttta ttatttatcc gtctctttta caaacaattc
      601 ttcgttagga ctgttgtaat aatttgaatt taatttaagt tctccgttgg aattgttttt
      661 tccaattacg gataatagca tggggctaaa acctaatatt cctagagaaa tagaacccag
      721 taccaacaca gtttttttta acataggtta tacctccact ttttttaacg ctaccacttg
      781 tatacacatt ttaaatacta gccttcagtt tatcaaatgt ttaaattgaa cggatataaa
      841 tcaaaattaa attttcaaaa gttgttctaa aaaatttttc ttttttggaa gttactctaa
      901 ctttttttgc ttgtggtgct tggtatgttt tcttcgccga ccgtttaggt tcttgaccat
      961 tttctcttat aagtgggatt tttaccacgt tgagagaaaa tggtcaagaa cctgggaggc
     1021 ggagaatgcc cttaatacca agcaccgcaa gcctttccca tactttattc gatgatccca
     1081 cgatgatctc ataatgattc tacaatgacc ctacgatgat caaaattctc aaaaaaaata
     1141 aacgaaattt caaacgattt ttttgaaatt ttgttaaaat cacatttgtt tttttatgac
     1201 tttttcaaca aaatcgatta gaatttattt tgtttttcca ccaaaaattg agtgagattt
     1261 caacctattt taagcaataa gtcaccatat agtttgatag tttacaacta taaacattga
     1321 ttgtttacag ttgtaaacta ttttttcttt ctcataaaaa tttgagtgat ctccccatca
     1381 ggtagtacaa ctttttcctc tactatccga actaacttct agaaaatttt agagattggt
     1441 tctattagat ttccaaattt gccaccactt tttttcaata agtggttgtg tattgtctgc
     1501 ttttttttca gaaaaggcag ttgattcggt tgttggagtt tcttctgtat aagttaaatg
     1561 gagctgttct tgtaggttct caatttgttg gttcgcttgt agagtgagtt gttgttgctg
     1621 gtcgagtaat ttttgcattt gttttatttg attatctttt tcttgtagct gtatatcttt
     1681 tcgatttaac tgtttttcta ggaaagaaag gagttttaat tcggaaactt ggttggtgtt
     1741 tttatggttt ttggtttttg atttttcgcc aactagtttt cgatttaagt ttgcgttatc
     1801 gactaaaaac attgatttta tagctttttg accggtttcg ttaatcacaa acttgtttcc
     1861 gttttttgca aaccaaagtt ttcgattttt gtcagaaact tttttgttaa ttgctgtttt
     1921 actaactcct aattcttccg caagttcttt gattgtttta tctgccataa atactcctta
     1981 agcttaatcg aataagtttt gtaaatttcc tagtaaatct tgtttgagtt tgtttgaaag
     2041 ttctcgtttt tctgcctgtt tttcttcagt ttgttgttgt tgtttaaaat cagcttcatg
     2101 agtgcctaag ggaagattta gtattcgatc tttagcgcgc catttttctt ctcgtgttaa
     2161 ttctccattg tgatcaatat taaataattt tatacgtaaa tcttcttgtt tccctttgga
     2221 aaaatcgtca gcatggttaa tttcagcttt ccaagtaaaa cgataaccga taacaggttt
     2281 acctcgacct ttaccatatt ttttacgaat agttaatcct ttaaataagg gagtgagttc
     2341 ttctttaatt ggccttaaga taaatttgtc aatatttgac ggactattcc aataactttt
     2401 aggaatatct agtaaatcaa aaaatttttc tttggaaaat tcagtccagc cttgagttcg
     2461 atattgtttt aataatcgaa acatagtttt tgaatatgtg ctatccaaat tagtaaattg
     2521 ctctagatta aatcttgtcc agttctttaa ttgattaaaa agtcctttaa attctggatt
     2581 aactgaaatt ataacaagtt gtttatctcg tataatgcta tattttgtga aagcaacaaa
     2641 tttagttaaa gtatttccat catcattcca agcattaata cttaataatt tgtcatacgt
     2701 gctactaaga tcttttataa attgttcccc acgttctgta taaccgctta gttttttcaa
     2761 atcgtgaaat gaaaaaataa tctcatctac acctttttca cgtacacgtg aagcaattga
     2821 aaaaaataag ttcatttcac gactattaaa ttttctcaga ggaatatgat ttaatctatt
     2881 ttcatatttt acaatttcat ttgccataag cttctcctac ttttataaag tatgaaataa
     2941 aagtaacata ctttataaaa atactcaata atgtcggaaa acctacttga aaagcgtaga
     3001 aaacctactt gaaaagcgta gaaaacctac ttgaaaagcg tagaaaacct acttgaaaaa
     3061 actctgaatt gcttgggaga gtaaggacac agccgacata aaagagatat aaaaagagat
     3121 ataaaaagag atataaaaag aggggaggct aattttcccg cctaaaaaat cactcctttt
     3181 ttctctaaac aggttcgcga atttatcaaa actgccaatt ccaaaccttg ctccctacgg
     3241 ttcgctaaaa aggtgtgatc gctctgctct cacattatac tgcgcagtcg cctccggctc
     3301 ctttgctaaa agccgttcat aaggaaccct ttgagaagct ctgagcgttc gtttaagaca
     3361 ttttaagaca gtcctagtat aaatccctta tcttgttctt aaaacgcctt acaggcggtc
     3421 agggcaagcc ctaacaaccc taaaattgtc taaatgccct ctaaaattcg atttaagcac
     3481 tcttaaacct attcttatat atttatatac ttaggcgttt caaacgtctt aaaacgcaaa
     3541 ttagagcttg ataaagatat ttgctcgtgt gcctagactt ttaaaaaagt cggcacacgc
     3601 catgacaaag ctgaaataaa ctgcttcagc tttgtcatgg cggattcgct cacttgaaaa
     3661 agtgtttatt tcgctcgatt attagcatat attttttagc ttttttgcta gccaaatttt
     3721 gcattaattt gctcataagt cccacccttt cagtacgtcc tacggacgta tcgttcgtaa
     3781 tgtgaaagta cctttaaagg tactttctac aaaactcact aaaagggttt ggcaagctct
     3841 atatttgcta gccaaatacc tccgtatttg gtgcaaataa ccgcttgtca aaggggcgca
     3901 agctccccct tgaatccccc tcaaaaccca cgctccacaa tacaaaaagt ggctagaaaa
     3961 tgctagccac tttttgtaaa atttagtatg atttattttt agctatttag agtttttatt
     4021 tccgtaaata cttctaaaaa ttgctaagat aaatgctata ggaattagat aaagaatact
     4081 tttttgtaag cttatattga aaattagcct aaatatgaag aacatagtga ttaaaactat
     4141 tattgaaata attacagcta aaatcttttt attcatgaga tagtttcact ttctgattat
     4201 tttattacat aacgagcgta atcattgtat tgatatgatg ttgtattgct aaagtgagca
     4261 ttatatgtta attgtacacc ttttccttga tttgcagctc ttacaaagac attcattgca
     4321 tcaacagcac tactaaaagc atttgtaata acccatgttc caactccacc aataggaata
     4381 aaagatgcaa tttgagcagg agtctttagc ttattataat tttttttcaa atctctacac
     4441 attttattag taacataata gttcat
//
